CELL COUNT AND DIFFERENTIAL, CSF

LAB212

CSFCTDIFF

Collect

At least 2 mL CSF in sterile tube, container or syringe.

Remove needle prior to transportation when submitting sample in a syringe. 

Unacceptable Conditions

Container with needle attached; frozen sample; sample not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab immediately at 2-8 degrees C.

Stability (from collection to initiation)

Ambient: 1 hour; Refrigerated: 1 hour; Frozen: Unacceptable.

Performed by

PCL Clinical Laboratory - Core Hematology

Performed

Upon receipt

Methodology

Microscopy

Reported

Same day

Synonyms

  • LAB212
  • CSFCTDIFF

Reference Interval

 
Component From Age To Age Normal Low Normal High Units
Appearance CSF 0 150 Clear Clear  
Color CSF 0 150 Colorless Colorless  
Nucleated Cell Count CSF


 
0 1m 0 30 10^6/L
1m 1 0 20 10^6/L
1 12 0 10 10^6/L
12 150 0 5 10^6/L
Red Blood Cell Count CSF 0 150 0 0 10^6/L
Bands CSF 0 150 0 0 %
Blasts CSF 0 150 0 0 %
Eosinophils CSF 0 150 0 0 %
Lymphocytes CSF
 
0 1m 5 35 %
1m 150 40 80 %
Monocytes/Macrophages CSF
 
0 1m 50 90 %
1m 150 15 45 %
Other Mononuclear Cells CSF 0 150 0 0 %
Segmented Neutrophils CSF
 
0 1m 0 8 %
1m 150 0 6 %

Performed by

PCL Clinical Laboratory - Core Hematology

CPT Codes

89051

Collection

LAB212

CSFCTDIFF

Collect

At least 2 mL CSF in sterile tube, container or syringe.

Remove needle prior to transportation when submitting sample in a syringe. 

Unacceptable Conditions

Container with needle attached; frozen sample; sample not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab immediately at 2-8 degrees C.

Stability (from collection to initiation)

Ambient: 1 hour; Refrigerated: 1 hour; Frozen: Unacceptable.

Performed by

PCL Clinical Laboratory - Core Hematology

Ordering

Performed

Upon receipt

Methodology

Microscopy

Reported

Same day

Synonyms

  • LAB212
  • CSFCTDIFF
Result Interpretation

Reference Interval

 
Component From Age To Age Normal Low Normal High Units
Appearance CSF 0 150 Clear Clear  
Color CSF 0 150 Colorless Colorless  
Nucleated Cell Count CSF


 
0 1m 0 30 10^6/L
1m 1 0 20 10^6/L
1 12 0 10 10^6/L
12 150 0 5 10^6/L
Red Blood Cell Count CSF 0 150 0 0 10^6/L
Bands CSF 0 150 0 0 %
Blasts CSF 0 150 0 0 %
Eosinophils CSF 0 150 0 0 %
Lymphocytes CSF
 
0 1m 5 35 %
1m 150 40 80 %
Monocytes/Macrophages CSF
 
0 1m 50 90 %
1m 150 15 45 %
Other Mononuclear Cells CSF 0 150 0 0 %
Segmented Neutrophils CSF
 
0 1m 0 8 %
1m 150 0 6 %

Performed by

PCL Clinical Laboratory - Core Hematology

Administrative

CPT Codes

89051

CELL COUNT, BODY FLUID

LAB209

BFCT

Collect

At least 2 mL of fluid in 4.0 mL purple top tube (EDTA) or other sterile tube, container or syringe.

Indicate body fluid source on requisition.

Remove needle prior to transportation when submitting sample in a syringe.

Unacceptable Conditions

Container with needle attached; incorrect container; frozen sample; sample not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal:  Deliver to lab immediately.
Offsite:  Transport to lab as soon as possible at 2-8 degrees C.

 

Stability (from collection to initiation)

Ambient: 1 hour;  Refrigerated: 24 hours;  Frozen: Unacceptable..

Remarks

Indicate body fluid SOURCE on requisition.

For synovial fluid, if crystals are suspected under light microscopy and their presence is confirmed using polarization, test LAB213 CRYSTAL ANALYSIS, SYNOVIAL FLUID will also be reported and billed.

Performed by

PCL Clinical Laboratory - Core Hematology

 

Performed

Upon receipt

Methodology

Automated and microscopic examination

Reported

Same Day

Synonyms

  • LAB209
  • CELL CT BF
  • BFCT

Reference Interval

The reference range an other method performance specifications have not been established for this test.  Results must be integrated into the clinical context for interpretation.

Performed by

PCL Clinical Laboratory - Core Hematology

 

CPT Codes

89050
If crystals are seen and confirmed, 89060 will also be billed.

Collection

LAB209

BFCT

Collect

At least 2 mL of fluid in 4.0 mL purple top tube (EDTA) or other sterile tube, container or syringe.

Indicate body fluid source on requisition.

Remove needle prior to transportation when submitting sample in a syringe.

Unacceptable Conditions

Container with needle attached; incorrect container; frozen sample; sample not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal:  Deliver to lab immediately.
Offsite:  Transport to lab as soon as possible at 2-8 degrees C.

 

Stability (from collection to initiation)

Ambient: 1 hour;  Refrigerated: 24 hours;  Frozen: Unacceptable..

Remarks

Indicate body fluid SOURCE on requisition.

For synovial fluid, if crystals are suspected under light microscopy and their presence is confirmed using polarization, test LAB213 CRYSTAL ANALYSIS, SYNOVIAL FLUID will also be reported and billed.

Performed by

PCL Clinical Laboratory - Core Hematology

 

Ordering

Performed

Upon receipt

Methodology

Automated and microscopic examination

Reported

Same Day

Synonyms

  • LAB209
  • CELL CT BF
  • BFCT
Result Interpretation

Reference Interval

The reference range an other method performance specifications have not been established for this test.  Results must be integrated into the clinical context for interpretation.

Performed by

PCL Clinical Laboratory - Core Hematology

 

Administrative

CPT Codes

89050
If crystals are seen and confirmed, 89060 will also be billed.

CELL COUNT, CSF

LAB3330

CSFCT

Collect

At least 2 mL CSF in sterile tube, container or syringe. Minimum volume: 0.5 mL.
Remove needle prior to transportation when submitting sample in a syringe.

Unacceptable Conditions

Container with needle attached; sample not properly identified; insufficient sample volume; incorrect container; frozen sample.

Storage/Transport Temperature

Internal: Deliver to laboratory immediately.
Offsite: Transport to lab immediately at 2-8 degrees C.

Stability (from collection to initiation)

Ambient: 1 hour;  Refrigerated: 1 hour;   Frozen: Unacceptable

Performed by

PCL Clinical Laboratory - Core Hematology

Performed

Upon receipt

Methodology

Microscopy

Reported

Same day

Synonyms

  • LAB3330
  • CSFCT
  • Cerebrospinal Fluid Cell Count

Reference Interval

Component From Age To Age Normal Low Normal High Units
Appearance 0 150 Clear Clear  
Color 0 150 Colorless Colorless  
Nucleated Cell Count 0 1m 0 30 10^6/L
  1m 1 0 20 10^6/L
  1 12 0 10 10^6/L
  12 150 0 5 10^6/L
Red Blood Cell Count 0 150 0 0 10^6/L

Performed by

PCL Clinical Laboratory - Core Hematology

CPT Codes

89050

Collection

LAB3330

CSFCT

Collect

At least 2 mL CSF in sterile tube, container or syringe. Minimum volume: 0.5 mL.
Remove needle prior to transportation when submitting sample in a syringe.

Unacceptable Conditions

Container with needle attached; sample not properly identified; insufficient sample volume; incorrect container; frozen sample.

Storage/Transport Temperature

Internal: Deliver to laboratory immediately.
Offsite: Transport to lab immediately at 2-8 degrees C.

Stability (from collection to initiation)

Ambient: 1 hour;  Refrigerated: 1 hour;   Frozen: Unacceptable

Performed by

PCL Clinical Laboratory - Core Hematology

Ordering

Performed

Upon receipt

Methodology

Microscopy

Reported

Same day

Synonyms

  • LAB3330
  • CSFCT
  • Cerebrospinal Fluid Cell Count
Result Interpretation

Reference Interval

Component From Age To Age Normal Low Normal High Units
Appearance 0 150 Clear Clear  
Color 0 150 Colorless Colorless  
Nucleated Cell Count 0 1m 0 30 10^6/L
  1m 1 0 20 10^6/L
  1 12 0 10 10^6/L
  12 150 0 5 10^6/L
Red Blood Cell Count 0 150 0 0 10^6/L

Performed by

PCL Clinical Laboratory - Core Hematology

Administrative

CPT Codes

89050

CERULOPLASMIN

LAB703

CERULOPLASMI

Collect

One 5.0 mL gold or red top with gel (SST), (clot activator serum).

Pediatric Collection

At least 1.0 mL of whole blood collected in gold or red top; or 0.5 mL serum in aliquot tube.

Unacceptable Conditions

Gross lipemia; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Allow to clot for 30 minutes, then deliver to lab immediately at ambient temperature.
Offsite: Allow to clot 30 minutes, immediately centrifuge, place at least 1.0 mL of serum in an aliquot container and transport to lab at 2-8 degrees C.

Stability (from collection to initiation)

Before centrifugation: 1 hour

After centrifugation: Ambient, 1 hour.

Serum aliquot: Ambient, 1 hour; Refrigerated, 7 days; Frozen (-20 degrees C), 3 months.

Performed by

PCL Clinical Laboratory - Special Chemistry

Performed

Thursday

Methodology

Immunonephelometry

Reported

Same day

Reference Interval

20.0-60.0 mg/dL

Performed by

PCL Clinical Laboratory - Special Chemistry

CPT Codes

82390

Collection

LAB703

CERULOPLASMI

Collect

One 5.0 mL gold or red top with gel (SST), (clot activator serum).

Pediatric Collection

At least 1.0 mL of whole blood collected in gold or red top; or 0.5 mL serum in aliquot tube.

Unacceptable Conditions

Gross lipemia; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Allow to clot for 30 minutes, then deliver to lab immediately at ambient temperature.
Offsite: Allow to clot 30 minutes, immediately centrifuge, place at least 1.0 mL of serum in an aliquot container and transport to lab at 2-8 degrees C.

Stability (from collection to initiation)

Before centrifugation: 1 hour

After centrifugation: Ambient, 1 hour.

Serum aliquot: Ambient, 1 hour; Refrigerated, 7 days; Frozen (-20 degrees C), 3 months.

Performed by

PCL Clinical Laboratory - Special Chemistry

Ordering

Performed

Thursday

Methodology

Immunonephelometry

Reported

Same day

Result Interpretation

Reference Interval

20.0-60.0 mg/dL

Performed by

PCL Clinical Laboratory - Special Chemistry

Administrative

CPT Codes

82390

CHC MOLECULAR E/H - RNA/DNA-SO

LAB4381

Collect

4.0 mL EDTA whole blood (or bone marrow), purple or pink

Specimen Preparation

1.0 mL minimum volume

Storage/Transport Temperature

Ambient or refrigerated.

 

Remarks

This test is for Bone Marrow Transplant doctors to save DNA and/or RNA for potential future testing when it is not clear what molecular testing is needed at the time of collection. Most often ordered on bone marrow biopsies.

Performed by

PCL Clinical Laboratory

Performed

As needed

Synonyms

  • LAB4381
  • Extract and Hold -DNA/RNA

Performed by

PCL Clinical Laboratory

Collection

LAB4381

Collect

4.0 mL EDTA whole blood (or bone marrow), purple or pink

Specimen Preparation

1.0 mL minimum volume

Storage/Transport Temperature

Ambient or refrigerated.

 

Remarks

This test is for Bone Marrow Transplant doctors to save DNA and/or RNA for potential future testing when it is not clear what molecular testing is needed at the time of collection. Most often ordered on bone marrow biopsies.

Performed by

PCL Clinical Laboratory

Ordering

Performed

As needed

Synonyms

  • LAB4381
  • Extract and Hold -DNA/RNA
Result Interpretation

Performed by

PCL Clinical Laboratory

Administrative

CHIMERISM BY SHORT TANDEM REPS

LAB3090

Collect

EDTA Whole blood (Pre-transplant);Bone marrow; EDTA Whole blood for CD3, CD33, CD565 enriched fractions (Post-transplant)

Whole Blood for Post-transplant "sorted" chimersim testing should not be submitted between Fridays @ 1200 thru Sunday @1200. 

Unacceptable Conditions

Frozen sample.

 

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 5.0 mL blood/1.0 mL bone marrow/20 mL blood for CD3, CD33,CD56 fractions

Deliver to lab at ambient temperature.

Stability (from collection to initiation)

Refrigerated: 3 days

Remarks

Testing to determine extent of bone marrow donor engraftment.

Performed by

The Children's Hospital - Aurora

Performed

Varies

Methodology

Polymerase chain reaction [PCR].

Reported

Varies

Synonyms

  • LAB3090

Performed by

The Children's Hospital - Aurora

CPT Codes

81265 (pre-transplant/recip&donor#1), 81267 (post-transplant), 81268 (post-transplant sorted fractions), 81266 (pre-transplant/donor#2)

Collection

LAB3090

Collect

EDTA Whole blood (Pre-transplant);Bone marrow; EDTA Whole blood for CD3, CD33, CD565 enriched fractions (Post-transplant)

Whole Blood for Post-transplant "sorted" chimersim testing should not be submitted between Fridays @ 1200 thru Sunday @1200. 

Unacceptable Conditions

Frozen sample.

 

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 5.0 mL blood/1.0 mL bone marrow/20 mL blood for CD3, CD33,CD56 fractions

Deliver to lab at ambient temperature.

Stability (from collection to initiation)

Refrigerated: 3 days

Remarks

Testing to determine extent of bone marrow donor engraftment.

Performed by

The Children's Hospital - Aurora

Ordering

Performed

Varies

Methodology

Polymerase chain reaction [PCR].

Reported

Varies

Synonyms

  • LAB3090
Result Interpretation

Performed by

The Children's Hospital - Aurora

Administrative

CPT Codes

81265 (pre-transplant/recip&donor#1), 81267 (post-transplant), 81268 (post-transplant sorted fractions), 81266 (pre-transplant/donor#2)

CHITOTRIOSIDASE

LAB3488

Collect

5.0 mL gold or red with gel (SST), (clot activated serum)

Storage/Transport Temperature

Ambient/Refrigerated:  Unacceptable

 Store Frozen

Performed by

Emory Genetics Lab

Methodology

Flurometric enzyme activity assay using artifical 4-MU substrate for Chitotriosidase.

Reported

7-10 days

Synonyms

  • GAPCLER BIOMARKER
  • LAB3488

Reference Interval

Normal range:  0-78.5 nmol/hr/mL (32 +/-46, n=23), Median 28 nmol/hr/mL

Performed by

Emory Genetics Lab

CPT Codes

82657

Collection

LAB3488

Collect

5.0 mL gold or red with gel (SST), (clot activated serum)

Storage/Transport Temperature

Ambient/Refrigerated:  Unacceptable

 Store Frozen

Performed by

Emory Genetics Lab

Ordering

Methodology

Flurometric enzyme activity assay using artifical 4-MU substrate for Chitotriosidase.

Reported

7-10 days

Synonyms

  • GAPCLER BIOMARKER
  • LAB3488
Result Interpretation

Reference Interval

Normal range:  0-78.5 nmol/hr/mL (32 +/-46, n=23), Median 28 nmol/hr/mL

Performed by

Emory Genetics Lab

Administrative

CPT Codes

82657

CHLAMYDIA AB PANEL - IGM

LAB1208

Collect

Plain red or serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, hemolyzed, or hyperlipemic sera.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Mon-Sat

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-4 days

Synonyms

  • LAB1208

Ordering Recommendations

A combined IgG and IgM antibody panel is available (refer to 0065100). Differentiate between Chlamydophila species (C. psittaci, C. pneumoniae). Differentiate early IgM response to infection from persistent low-level titer. Because of cross-reactivity, a C. pneumoniae-specific reaction will exhibit titers two-fold or greater than C. trachomatis or C. psittaci serology. Limited value in the diagnosis of most oculogenital (eg, eyes, genitalia) chlamydial infections.

Reference Interval

< 1:20 C. trachomatis IgM.
< 1:20 C. pneumoniae IgM.
< 1:20 C. psittaci IgM.

Interpretive Data

The Chlamydia antibody test contains both species- and genus-specific antigens, and serological cross-reactions may be seen in both acute and convalescent samples (less than 1:128).  A C. pneumoniae-specific reaction will exhibit titers twofold or greater than titers observed with C. trachomatis or C. psittaci serology.  Any IgG titer may indicate past exposure to that particular species.  IgG titers in recently infected individuals are typically greater than or equal to 1:512. 

The Chlamydia microimmunofluorescent assay utilizes C. psittaci, C. pneumoniae, and nine serotypes of C. trachomatis. It does not include the LGV strains of C. trachomatis.

CPT Codes

86632 x3
Collection

LAB1208

Collect

Plain red or serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, hemolyzed, or hyperlipemic sera.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Mon-Sat

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-4 days

Synonyms

  • LAB1208

Ordering Recommendations

A combined IgG and IgM antibody panel is available (refer to 0065100). Differentiate between Chlamydophila species (C. psittaci, C. pneumoniae). Differentiate early IgM response to infection from persistent low-level titer. Because of cross-reactivity, a C. pneumoniae-specific reaction will exhibit titers two-fold or greater than C. trachomatis or C. psittaci serology. Limited value in the diagnosis of most oculogenital (eg, eyes, genitalia) chlamydial infections.
Result Interpretation

Reference Interval

< 1:20 C. trachomatis IgM.
< 1:20 C. pneumoniae IgM.
< 1:20 C. psittaci IgM.

Interpretive Data

The Chlamydia antibody test contains both species- and genus-specific antigens, and serological cross-reactions may be seen in both acute and convalescent samples (less than 1:128).  A C. pneumoniae-specific reaction will exhibit titers twofold or greater than titers observed with C. trachomatis or C. psittaci serology.  Any IgG titer may indicate past exposure to that particular species.  IgG titers in recently infected individuals are typically greater than or equal to 1:512. 

The Chlamydia microimmunofluorescent assay utilizes C. psittaci, C. pneumoniae, and nine serotypes of C. trachomatis. It does not include the LGV strains of C. trachomatis.

Administrative

CPT Codes

86632 x3

CHLAMYDIA ANTIBODIES IGG/IGM

LAB790

Collect

Plain red or serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, hemolyzed, or hyperlipemic sera.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Sun-Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-4 days

Synonyms

  • LAB790

Ordering Recommendations

Differentiate between Chlamydophila species (C. psittaci, C. pneumoniae). Differentiate early IgM response to infection from persistent low-level titer. Because of cross-reactivity, a C. pneumoniae-specific reaction will exhibit titers two-fold or greater than C. trachomatis or C. psittaci serology. Limited value in the diagnosis of most oculogenital (eg, eyes, genitalia) chlamydial infections.

Reference Interval

< 1:64 C. pneumoniae IgG.     
< 1:64 C. psittaci IgG.
< 1:64 C. trachomatis IgG.
< 1:20 C. pneumoniae IgM.     
< 1:20 C. psittaci IgM.
< 1:20 C. trachomatis IgM.

Interpretive Data

Refer to individual components.

CPT Codes

86631 x3; 86632 x3
Collection

LAB790

Collect

Plain red or serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, hemolyzed, or hyperlipemic sera.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Sun-Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-4 days

Synonyms

  • LAB790

Ordering Recommendations

Differentiate between Chlamydophila species (C. psittaci, C. pneumoniae). Differentiate early IgM response to infection from persistent low-level titer. Because of cross-reactivity, a C. pneumoniae-specific reaction will exhibit titers two-fold or greater than C. trachomatis or C. psittaci serology. Limited value in the diagnosis of most oculogenital (eg, eyes, genitalia) chlamydial infections.
Result Interpretation

Reference Interval

< 1:64 C. pneumoniae IgG.     
< 1:64 C. psittaci IgG.
< 1:64 C. trachomatis IgG.
< 1:20 C. pneumoniae IgM.     
< 1:20 C. psittaci IgM.
< 1:20 C. trachomatis IgM.

Interpretive Data

Refer to individual components.

Administrative

CPT Codes

86631 x3; 86632 x3

More Articles...