Cystic Fibrosis Respiratory Culture (includes Gram Stain)

Collect

2-5 mL of expectorated sputum, tracheal aspirate, induced sputum, BAL, or bronch brush in saline, in a sterile container.

Source must be indicated. Indicate any unusual suspected organisms or if patient has lung transplant.

For non-cystic fibrosis, non-lung transplant patients, order C RESP.

Pediatric Collection

2 mL minimum volume.

Unacceptable Conditions

Poor quality sputum; subsequent samples during the same 24-hour period; sample not properly identified; non-sterile or leaking container.

Storage/Transport Temperature

Internal: Remove suction tubing top from sterile container and replace with cap included in package. Place each specimen in a sealed individual bag. Deliver to lab immediately at ambient temperature.

Offsite: Package as above  and send at 2-8 degrees C within 24 hours of collection. Do not freeze.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 24 hours; Frozen: Unacceptable.

Remarks

Care provider will be notified of unacceptable specimens.

Identification and susceptibility testing will be performed according to evidence-based practices.

Cultures will be held for 7 days.

Performed by

PCL Clinical Laboratory - Microbiology

Notes

Gram stain, identification, and susceptibility tests are billed separately from culture.

Routine bacterial culture only. For other requests, order specific culture (e.g. C AFB for Mycobacterial culture).

Performed

Daily

Methodology

Standard microbiology procedures for bacterial stain, aerobic culture and identification.

Reported

Preliminary: 1 day

Final: Negative at 2 days

Synonyms

  • LAB3696

Interpretive Data

Usual respiratory flora

Performed by

PCL Clinical Laboratory - Microbiology

CPT Codes

87070 Routine Bacterial Culture

Collection

Collect

2-5 mL of expectorated sputum, tracheal aspirate, induced sputum, BAL, or bronch brush in saline, in a sterile container.

Source must be indicated. Indicate any unusual suspected organisms or if patient has lung transplant.

For non-cystic fibrosis, non-lung transplant patients, order C RESP.

Pediatric Collection

2 mL minimum volume.

Unacceptable Conditions

Poor quality sputum; subsequent samples during the same 24-hour period; sample not properly identified; non-sterile or leaking container.

Storage/Transport Temperature

Internal: Remove suction tubing top from sterile container and replace with cap included in package. Place each specimen in a sealed individual bag. Deliver to lab immediately at ambient temperature.

Offsite: Package as above  and send at 2-8 degrees C within 24 hours of collection. Do not freeze.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 24 hours; Frozen: Unacceptable.

Remarks

Care provider will be notified of unacceptable specimens.

Identification and susceptibility testing will be performed according to evidence-based practices.

Cultures will be held for 7 days.

Performed by

PCL Clinical Laboratory - Microbiology

Notes

Gram stain, identification, and susceptibility tests are billed separately from culture.

Routine bacterial culture only. For other requests, order specific culture (e.g. C AFB for Mycobacterial culture).

Ordering

Performed

Daily

Methodology

Standard microbiology procedures for bacterial stain, aerobic culture and identification.

Reported

Preliminary: 1 day

Final: Negative at 2 days

Synonyms

  • LAB3696
Result Interpretation

Interpretive Data

Usual respiratory flora

Performed by

PCL Clinical Laboratory - Microbiology

Administrative

CPT Codes

87070 Routine Bacterial Culture

CYSTICERCOSIS AB IGG - CSF

LAB1083

Collect

CSF.

Specimen Preparation

Transfer 1 mL CSF to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Unacceptable Conditions

Serum. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Mon

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-8 days

Synonyms

  • Taenia solium Antibody IgG (CSF) by ELISA
  • LAB1083

Ordering Recommendations

Detect the presence of CSF IgG antibodies to T. solium, if clinical suspicion of cysticercosis exists. Confirm positive results with IgG Western blot antibody test.

Reference Interval

0.34 O.D. or less:  Negative - No significant level of cysticercosis IgG antibody detected.
0.35-0.50 O.D.: Equivocal - Questionable presence of cysticercosis IgG.  Repeat testing in 10-14 days may be helpful.
0.51 O.D. or greater: Positive - IgG antibody to cysticercosis detected, which may suggest current or past infection.

Interpretive Data

Diagnosis of central nervous system infections can be accomplished by demonstrating the presence of intrathecally-produced specific antibody. Interpretation of results may be complicated by low antibody levels found in CSF, passive transfer of antibody from blood, and contamination via bloody taps.

CPT Codes

86682
Collection

LAB1083

Collect

CSF.

Specimen Preparation

Transfer 1 mL CSF to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Unacceptable Conditions

Serum. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Mon

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-8 days

Synonyms

  • Taenia solium Antibody IgG (CSF) by ELISA
  • LAB1083

Ordering Recommendations

Detect the presence of CSF IgG antibodies to T. solium, if clinical suspicion of cysticercosis exists. Confirm positive results with IgG Western blot antibody test.
Result Interpretation

Reference Interval

0.34 O.D. or less:  Negative - No significant level of cysticercosis IgG antibody detected.
0.35-0.50 O.D.: Equivocal - Questionable presence of cysticercosis IgG.  Repeat testing in 10-14 days may be helpful.
0.51 O.D. or greater: Positive - IgG antibody to cysticercosis detected, which may suggest current or past infection.

Interpretive Data

Diagnosis of central nervous system infections can be accomplished by demonstrating the presence of intrathecally-produced specific antibody. Interpretation of results may be complicated by low antibody levels found in CSF, passive transfer of antibody from blood, and contamination via bloody taps.

Administrative

CPT Codes

86682

CYSTICERCOSIS AB IGG -SERUM

LAB1082

Collect

Serum separator tube.

Specimen Preparation

Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

CSF. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Mon

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-8 days

Synonyms

  • Taenia solium Antibody IgG by ELISA
  • LAB1082
  • CYST S

Ordering Recommendations

Detect presence of IgG antibodies to T. solium in serum if clinical suspicion of cysticercosis exists.

Reference Interval

0.34 O.D. or less: Negative - No significant level of cysticercosis IgG antibody detected.
0.35-0.50 O.D.: Equivocal - Questionable presence of cysticercosis IgG antibody detected.  Repeat testing in 10-14 days may be helpful.
0.51 O.D. or greater: Positive -  IgG antibody to cysticercosis detected suggestive of current or past infection.

Interpretive Data

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.

Patients with collagen vascular diseases, hepatic cirrhosis, schistosomiasis, and other parasitic infections can produce false-positive results. There is a strong cross-reaction between cysticercosis and echinococcosis positive sera. Confirmation of positive ELISA results by the cysticercosis antibody, IgG by Western blot is recommended.

CPT Codes

86682
Collection

LAB1082

Collect

Serum separator tube.

Specimen Preparation

Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

CSF. Contaminated, heat-inactivated, hemolyzed, icteric, or lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Mon

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-8 days

Synonyms

  • Taenia solium Antibody IgG by ELISA
  • LAB1082
  • CYST S

Ordering Recommendations

Detect presence of IgG antibodies to T. solium in serum if clinical suspicion of cysticercosis exists.
Result Interpretation

Reference Interval

0.34 O.D. or less: Negative - No significant level of cysticercosis IgG antibody detected.
0.35-0.50 O.D.: Equivocal - Questionable presence of cysticercosis IgG antibody detected.  Repeat testing in 10-14 days may be helpful.
0.51 O.D. or greater: Positive -  IgG antibody to cysticercosis detected suggestive of current or past infection.

Interpretive Data

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.

Patients with collagen vascular diseases, hepatic cirrhosis, schistosomiasis, and other parasitic infections can produce false-positive results. There is a strong cross-reaction between cysticercosis and echinococcosis positive sera. Confirmation of positive ELISA results by the cysticercosis antibody, IgG by Western blot is recommended.

Administrative

CPT Codes

86682

CYTOMEGALOVIRUS CULTURE

Collect

Whole blood: Collect one 5.0 mL purple top, EDTA whole blood.

Respiratory, urine, tissue, body fluid, stool: Collect at least 1 mL in M6 Transport Media* or a sterile container.

*Specimens collected on swabs must be transported in M6 Transport Media.

Unacceptable Conditions

Specimens in transport media at 2-8 degrees C for over 48 hours; specimens at ambient temperature over 2 hours; dry swabs, swabs not in transport media; calcium alginate swabs; wood-shafted swabs; specimens not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport same day refrigerated or on ice. If delivery is not expected within 24 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated (sterile container): 24 hours; Refrigerated (transport media): 48 hours; Frozen (-70 degrees C): Greater than 48 hours.

Temperature labile organism.

Remarks

Specimens should be cultured within 24hrs for best recovery.

Performed by

PCL Clinical Laboratory - Virology

Performed

Daily

Methodology

Spin enhanced tissue culture and staining with fluorescein-labeled monoclonal antibodies.

Reported

In 2 days

Performed by

PCL Clinical Laboratory - Virology

CPT Codes

87254
Collection

Collect

Whole blood: Collect one 5.0 mL purple top, EDTA whole blood.

Respiratory, urine, tissue, body fluid, stool: Collect at least 1 mL in M6 Transport Media* or a sterile container.

*Specimens collected on swabs must be transported in M6 Transport Media.

Unacceptable Conditions

Specimens in transport media at 2-8 degrees C for over 48 hours; specimens at ambient temperature over 2 hours; dry swabs, swabs not in transport media; calcium alginate swabs; wood-shafted swabs; specimens not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport same day refrigerated or on ice. If delivery is not expected within 24 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated (sterile container): 24 hours; Refrigerated (transport media): 48 hours; Frozen (-70 degrees C): Greater than 48 hours.

Temperature labile organism.

Remarks

Specimens should be cultured within 24hrs for best recovery.

Performed by

PCL Clinical Laboratory - Virology
Ordering

Performed

Daily

Methodology

Spin enhanced tissue culture and staining with fluorescein-labeled monoclonal antibodies.

Reported

In 2 days
Result Interpretation

Performed by

PCL Clinical Laboratory - Virology
Administrative

CPT Codes

87254

CYTOMEGALOVIRUS IGG ANTIBODY

LAB467

CMV IgG

Collect

One 4.5 mL gold or red top with gel (SST), (clot activator serum).

Also acceptable: One 6.0 mL red top no gel, serum (clot activator).

Pediatric Collection

At least 1.0 mL blood in a 5.0 mL gold or red top with gel (SST), (clot activator serum) or other serum tube.

Unacceptable Conditions

Grossly hemolyzed, icteric or lipemic samples; plasma samples; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Allow to clot for 30 minutes, then deliver to lab at ambient temperature.
Offsite: Allow to clot for 30 minutes, immediately centrifuge, place at least 2 mL of serum in an aliquot container. If tested within 2 days of collection, transport at 2-8 degrees C. If tested more than 2 days after collection, transport frozen.

Stability (from collection to initiation)

Before centrifugation: 1 hour

 

After centrifugation: Ambient, 1 hour; Refrigerated, 1 hour

Serum aliquot: Ambient, 1 hour; Refrigerated, 2 days; Frozen (-20 degrees C), 1 month.

Remarks

Serum with results interpreted as equivocal may contain very low levels of IgG. Submit a second specimen for verification.

Performed by

PCL Clinical Laboratory - Special Chemistry

Performed

Tuesday

 

 

Methodology

Chemiluminescent immunoassay (CLIA)

Reported

Tuesday

Synonyms

  • LAB467
  • CMV IgG

Reference Interval

Negative

Performed by

PCL Clinical Laboratory - Special Chemistry

CPT Codes

86644

Collection

LAB467

CMV IgG

Collect

One 4.5 mL gold or red top with gel (SST), (clot activator serum).

Also acceptable: One 6.0 mL red top no gel, serum (clot activator).

Pediatric Collection

At least 1.0 mL blood in a 5.0 mL gold or red top with gel (SST), (clot activator serum) or other serum tube.

Unacceptable Conditions

Grossly hemolyzed, icteric or lipemic samples; plasma samples; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Allow to clot for 30 minutes, then deliver to lab at ambient temperature.
Offsite: Allow to clot for 30 minutes, immediately centrifuge, place at least 2 mL of serum in an aliquot container. If tested within 2 days of collection, transport at 2-8 degrees C. If tested more than 2 days after collection, transport frozen.

Stability (from collection to initiation)

Before centrifugation: 1 hour

 

After centrifugation: Ambient, 1 hour; Refrigerated, 1 hour

Serum aliquot: Ambient, 1 hour; Refrigerated, 2 days; Frozen (-20 degrees C), 1 month.

Remarks

Serum with results interpreted as equivocal may contain very low levels of IgG. Submit a second specimen for verification.

Performed by

PCL Clinical Laboratory - Special Chemistry

Ordering

Performed

Tuesday

 

 

Methodology

Chemiluminescent immunoassay (CLIA)

Reported

Tuesday

Synonyms

  • LAB467
  • CMV IgG
Result Interpretation

Reference Interval

Negative

Performed by

PCL Clinical Laboratory - Special Chemistry

Administrative

CPT Codes

86644

CYTOMEGALOVIRUS IGM ANTIBODY

LAB957

CMV IGM

 

Collect

One 4.5 mL gold or red top with gel (SST), (clot activator serum).

Also acceptable: One 6.0 mL red top no gel, serum (clot activator). 

Pediatric Collection

At least 1.0 mL blood collected in a 4.5 mL gold or red top with gel (SST), (clot activator serum) or other serum tube.

Unacceptable Conditions

Plasma samples; grossly contaminated, hemolyzed, lipemic or icteric samples; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Allow to clot for 30 minutes, then deliver to lab at ambient temperature.

Offsite: Allow to clot for 30 minutes, immediately centrifuge, place at least 2 mL of serum in an aliquot container. If tested within 24 hours of collection, transport at 2-8 degrees C. If tested more than 24 hours after collection, transport frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 1 hour

After centrifugation: Ambient, 1 hour; Refrigerated, 1 hour.

Serum aliquot: Ambient, 1 hour; Refrigerated, 2 days; Frozen (-20 degrees C), 1 month.

Remarks

Serum with results interpreted as equivocal may contain very low levels of IgM. Submit a second sample for verification.

Performed by

PCL Clinical Laboratory - Special Chemistry

Performed

Tuesday

Methodology

Chemiluminescent immunoassay (CLIA).

Reported

Tuesday

Synonyms

  • LAB957
  • CMV IGM

Reference Interval

Negative

Performed by

PCL Clinical Laboratory - Special Chemistry

CPT Codes

86645

Collection

LAB957

CMV IGM

 

Collect

One 4.5 mL gold or red top with gel (SST), (clot activator serum).

Also acceptable: One 6.0 mL red top no gel, serum (clot activator). 

Pediatric Collection

At least 1.0 mL blood collected in a 4.5 mL gold or red top with gel (SST), (clot activator serum) or other serum tube.

Unacceptable Conditions

Plasma samples; grossly contaminated, hemolyzed, lipemic or icteric samples; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Allow to clot for 30 minutes, then deliver to lab at ambient temperature.

Offsite: Allow to clot for 30 minutes, immediately centrifuge, place at least 2 mL of serum in an aliquot container. If tested within 24 hours of collection, transport at 2-8 degrees C. If tested more than 24 hours after collection, transport frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 1 hour

After centrifugation: Ambient, 1 hour; Refrigerated, 1 hour.

Serum aliquot: Ambient, 1 hour; Refrigerated, 2 days; Frozen (-20 degrees C), 1 month.

Remarks

Serum with results interpreted as equivocal may contain very low levels of IgM. Submit a second sample for verification.

Performed by

PCL Clinical Laboratory - Special Chemistry

Ordering

Performed

Tuesday

Methodology

Chemiluminescent immunoassay (CLIA).

Reported

Tuesday

Synonyms

  • LAB957
  • CMV IGM
Result Interpretation

Reference Interval

Negative

Performed by

PCL Clinical Laboratory - Special Chemistry

Administrative

CPT Codes

86645