TYPE AND SCREEN - AUTOMATED

Collect

One 6.0 mL Pink top (EDTA) labeled with  patient's last name, first name, medical record number, date drawn, phlebotomist's initials, and second person verifier's initials.

Utilizing READBACK VERIFICATION, the phlebotomist and a second hospital personnel verify that the patient's identifiers on his/her hospital identification bracelet match those on the blood specimen. Both persons must initial the pink top tube and, if used, the pink Transfusion Service request form.

In the outpatient setting, it is permissible for the patient to verify his/her identification.

Also acceptable: One 4 mL Purple top (EDTA) labeled as above.

Tube must be filled to at least 80% capacity.

Pediatric Collection

3 mL minimum volume.

Unacceptable Conditions

Specimen not properly identified/labeled; incorrect container; insufficient sample volume; specimen grossly hemolyzed.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: Transport at ambient temperature within 8 hours, or refrigerated within 24 hours.

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 72 hours.

Remarks

Test ordered when anticipated blood usage is unlikely. Order Type & Crossmatch if transfusion is needed. If blood should be needed for transfusion subsequent to performance of type & screen, call the Blood Bank. The crossmatch will then be performed.

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

Notes

Forms requesting blood/components and forms accompanying blood samples from the recipient must contain sufficient information for positive identification of the recipient.

Joint Commission requires that the upper half of the Transfusion Service form be completed. This area requires the person requesting the blood or blood product transfusion and type and screen to have a justification of why the blood and/or products are being requested (check mark on the appropriate box on upper left side of the form).

This request form is considered a prescription for blood and/or blood products; to comply with the FDA, the name of both the ordering physician and the attending physician must be written on the form.

Before a specimen is used for blood grouping, typing, or compatibility testing, a qualified person in the Transfusion Service confirms that all identifying information on the request form is in agreement with that on the specimen label. If the specimen is mislabeled or unlabeled, a new specimen must be drawn.

Performed

Daily

Methodology

Galileo Echo

Reported

Daily

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

CPT Codes

86850; 86900; 86901
Collection

Collect

One 6.0 mL Pink top (EDTA) labeled with  patient's last name, first name, medical record number, date drawn, phlebotomist's initials, and second person verifier's initials.

Utilizing READBACK VERIFICATION, the phlebotomist and a second hospital personnel verify that the patient's identifiers on his/her hospital identification bracelet match those on the blood specimen. Both persons must initial the pink top tube and, if used, the pink Transfusion Service request form.

In the outpatient setting, it is permissible for the patient to verify his/her identification.

Also acceptable: One 4 mL Purple top (EDTA) labeled as above.

Tube must be filled to at least 80% capacity.

Pediatric Collection

3 mL minimum volume.

Unacceptable Conditions

Specimen not properly identified/labeled; incorrect container; insufficient sample volume; specimen grossly hemolyzed.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: Transport at ambient temperature within 8 hours, or refrigerated within 24 hours.

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 72 hours.

Remarks

Test ordered when anticipated blood usage is unlikely. Order Type & Crossmatch if transfusion is needed. If blood should be needed for transfusion subsequent to performance of type & screen, call the Blood Bank. The crossmatch will then be performed.

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

Notes

Forms requesting blood/components and forms accompanying blood samples from the recipient must contain sufficient information for positive identification of the recipient.

Joint Commission requires that the upper half of the Transfusion Service form be completed. This area requires the person requesting the blood or blood product transfusion and type and screen to have a justification of why the blood and/or products are being requested (check mark on the appropriate box on upper left side of the form).

This request form is considered a prescription for blood and/or blood products; to comply with the FDA, the name of both the ordering physician and the attending physician must be written on the form.

Before a specimen is used for blood grouping, typing, or compatibility testing, a qualified person in the Transfusion Service confirms that all identifying information on the request form is in agreement with that on the specimen label. If the specimen is mislabeled or unlabeled, a new specimen must be drawn.

Ordering

Performed

Daily

Methodology

Galileo Echo

Reported

Daily
Result Interpretation

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)
Administrative

CPT Codes

86850; 86900; 86901

TYPHUS FEVER ANTIBODY - IGG

LAB3186

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.05 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Tue, Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-5 days

Synonyms

  • Typhus Fever Antibody, IgG
  • LAB3186
  • TYPHUS IGG

Ordering Recommendations

Confirm presence of Rickettsia typhi. Panel test (IgG and IgM) is preferred. Requires comparison of acute- to convalescent-phase serology.

Reference Interval

< 1:64  Negative - No significant level of Rickettsia typhi IgG antibody detected.
1:64-1:128  Equivocal - Questionable presence of Rickettsia typhi IgG antibody detected.  Repeat testing in 10-14 days may be helpful.
≥ 1:256  Positive - Presence of IgG antibody to Rickettsia typhi detected, suggestive of current or past infection.

Interpretive Data

This test is for antibodies to Rickettsia typhi.  Any antibody reactivity to Rickettsia typhi antigen should, however, also be considered group-reactive for the Typhus Fever group (Rickettsia prowazekii). Seroconversion between acute and convalescent sera is considered strong evidence of recent infection.  The best evidence for infection is a significant change (fourfold difference in titer) on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

CPT Codes

86757
Collection

LAB3186

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.05 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Tue, Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-5 days

Synonyms

  • Typhus Fever Antibody, IgG
  • LAB3186
  • TYPHUS IGG

Ordering Recommendations

Confirm presence of Rickettsia typhi. Panel test (IgG and IgM) is preferred. Requires comparison of acute- to convalescent-phase serology.
Result Interpretation

Reference Interval

< 1:64  Negative - No significant level of Rickettsia typhi IgG antibody detected.
1:64-1:128  Equivocal - Questionable presence of Rickettsia typhi IgG antibody detected.  Repeat testing in 10-14 days may be helpful.
≥ 1:256  Positive - Presence of IgG antibody to Rickettsia typhi detected, suggestive of current or past infection.

Interpretive Data

This test is for antibodies to Rickettsia typhi.  Any antibody reactivity to Rickettsia typhi antigen should, however, also be considered group-reactive for the Typhus Fever group (Rickettsia prowazekii). Seroconversion between acute and convalescent sera is considered strong evidence of recent infection.  The best evidence for infection is a significant change (fourfold difference in titer) on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

Administrative

CPT Codes

86757

TYPHUS FEVER ANTIBODY - IGM

LAB3187

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic, specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Tue, Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-5 days

Synonyms

  • Typhus Fever Antibody, IgM
  • LAB3187
  • TYPHUS IGM

Ordering Recommendations

Confirm presence of Rickettsia typhi. Panel test (IgG and IgM) is preferred. Requires comparison of acute- to convalescent-phase serology.

Reference Interval

< 1:64  Negative - No significant level of Rickettsia typhi IgM antibody detected.
≥1:64  Positive - Presence of IgM antibody to Rickettsia typhi detected, suggestive of recent infection.

Interpretive Data

This test is for antibodies to Rickettsia typhi.  Any antibody reactivity to Rickettsia typhi antigen should, however, also be considered group-reactive for the Typhus Fever group (Rickettsia prowazekii).  Seroconversions or an increase between acute and convalescent IgG and IgM titers of at least fourfold is considered strong evidence of recent infection.

While the presence of IgM antibodies suggests recent infection, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

CPT Codes

86757
Collection

LAB3187

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic, specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Tue, Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-5 days

Synonyms

  • Typhus Fever Antibody, IgM
  • LAB3187
  • TYPHUS IGM

Ordering Recommendations

Confirm presence of Rickettsia typhi. Panel test (IgG and IgM) is preferred. Requires comparison of acute- to convalescent-phase serology.
Result Interpretation

Reference Interval

< 1:64  Negative - No significant level of Rickettsia typhi IgM antibody detected.
≥1:64  Positive - Presence of IgM antibody to Rickettsia typhi detected, suggestive of recent infection.

Interpretive Data

This test is for antibodies to Rickettsia typhi.  Any antibody reactivity to Rickettsia typhi antigen should, however, also be considered group-reactive for the Typhus Fever group (Rickettsia prowazekii).  Seroconversions or an increase between acute and convalescent IgG and IgM titers of at least fourfold is considered strong evidence of recent infection.

While the presence of IgM antibodies suggests recent infection, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Administrative

CPT Codes

86757

TYROSINE

LAB1103

Collect

2 mL green top tube 2 mL red top tube with or without gel

Pediatric Collection

0.3 mL

Storage/Transport Temperature

Internal: Deliver to lab immediately at room temperature
Offsite: 0.5 mL plasma/serum. Ship heparinized plasma/serum frozen.

Performed by

UCD Biochemical Genetics Laboratory

Performed

Weekly (Dayshift), day varies

Reported

7 - 10 days

Synonyms

  • LAB1103

Performed by

UCD Biochemical Genetics Laboratory

CPT Codes

82136

Collection

LAB1103

Collect

2 mL green top tube 2 mL red top tube with or without gel

Pediatric Collection

0.3 mL

Storage/Transport Temperature

Internal: Deliver to lab immediately at room temperature
Offsite: 0.5 mL plasma/serum. Ship heparinized plasma/serum frozen.

Performed by

UCD Biochemical Genetics Laboratory

Ordering

Performed

Weekly (Dayshift), day varies

Reported

7 - 10 days

Synonyms

  • LAB1103
Result Interpretation

Performed by

UCD Biochemical Genetics Laboratory

Administrative

CPT Codes

82136

TYSABRI ANTIBODY TEST

LAB3124

Collect

Plain red. Also acceptable: Serum separator tube (SST).

Specimen Preparation

Allow blood to clot at room temperature for 30 minutes. Separate serum from cells within 1 hour. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Frozen. Also acceptable: Refrigerated

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 2 Weeks; Frozen: 2 Weeks

Performed by

Effective: 9/20/12 

Viracor-IBT via ARUP

Performed

Varies

Methodology

Qualitative Bridging Enzyme-Linked Immunosorbent Assay

Reported

5-10 days

Synonyms

  • LAB3124
  • NATAL ABS

Ordering Recommendations

Aids in management of individuals receiving natalizumab therapy.

Reference Interval

By report

Performed by

Effective: 9/20/12 

Viracor-IBT via ARUP

CPT Codes

83516
Collection

LAB3124

Collect

Plain red. Also acceptable: Serum separator tube (SST).

Specimen Preparation

Allow blood to clot at room temperature for 30 minutes. Separate serum from cells within 1 hour. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Frozen. Also acceptable: Refrigerated

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 2 Weeks; Frozen: 2 Weeks

Performed by

Effective: 9/20/12 

Viracor-IBT via ARUP

Ordering

Performed

Varies

Methodology

Qualitative Bridging Enzyme-Linked Immunosorbent Assay

Reported

5-10 days

Synonyms

  • LAB3124
  • NATAL ABS

Ordering Recommendations

Aids in management of individuals receiving natalizumab therapy.
Result Interpretation

Reference Interval

By report

Performed by

Effective: 9/20/12 

Viracor-IBT via ARUP

Administrative

CPT Codes

83516