TOBRAMYCIN RANDOM

LAB37

TOB R

Collect

One 4.5 mL Green top tube with gel separator (PST).

Pediatric Collection

1.5 mL whole blood or 20 full capillary tubes.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection. Send at 2-8 degrees C if tested within 24 hours. Place plasma in aliquot container and send frozen if not tested within 24 hours.

Stability (from collection to initiation)

Refrigerated: 24 hours; Frozen (-20 degrees C): Greater than 24 hours.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Enzymatic

Reported

Same day

Synonyms

  • LAB37
  • TOB R

Interpretive Data

Effective 05/20/2014:

Random levels cannot be interpreted.  Contact the Infectious Disease Fellow for assistance.   Results from samples collected 7–14 hours after dosing can be plotted on a published nomogram to determine the proper dosing interval.

 

Before 05/20/2014:

Random levels cannot be interpreted. Call Infectious Disease Fellow for
assistance.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

80200

Collection

LAB37

TOB R

Collect

One 4.5 mL Green top tube with gel separator (PST).

Pediatric Collection

1.5 mL whole blood or 20 full capillary tubes.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection. Send at 2-8 degrees C if tested within 24 hours. Place plasma in aliquot container and send frozen if not tested within 24 hours.

Stability (from collection to initiation)

Refrigerated: 24 hours; Frozen (-20 degrees C): Greater than 24 hours.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Enzymatic

Reported

Same day

Synonyms

  • LAB37
  • TOB R
Result Interpretation

Interpretive Data

Effective 05/20/2014:

Random levels cannot be interpreted.  Contact the Infectious Disease Fellow for assistance.   Results from samples collected 7–14 hours after dosing can be plotted on a published nomogram to determine the proper dosing interval.

 

Before 05/20/2014:

Random levels cannot be interpreted. Call Infectious Disease Fellow for
assistance.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

80200

TOBRAMYCIN TROUGH

LAB38

TOB T

Collect

One 4.5 mL Green top tube with gel separator (PST).

Pediatric Collection

1.5 mL whole blood or 20 full capillary tubes.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection. Send at 2-8 degrees C if tested within 24 hours. Place plasma in aliquot container and send frozen if not tested within 24 hours.

Stability (from collection to initiation)

Refrigerated: 24 hours; Frozen (-20 degreees C): Greater than 24 hours.

Remarks

Draw trough level immediately before the next dose.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Enzymatic

Reported

Same day

Synonyms

  • LAB38

Reference Interval

Effective 05/20/2014:

0 - 2 ug/mL

Interpretive Data

Effective 05/20/2014:

Reports show that in patients on divided daily dosage regimens, trough concentrations of  1–2  μg/mL  usually  ensure  that  the  concentration  is  above  the  MIC of most tobramycin-sensitive pathogens and that the drug elimination is adequate.  When patients are on a QD regimen, reports show that trough concentrations less than 1 μg/mL allow adequate clearance before the next dose.  Prolonged  trough  concentrations  above  2  μg/mL  are  often  associated  with  renal  impairment and ototoxicity.  For effective treatment, some patients may require serum or plasma levels outside these ranges. Therefore, the expected ranges are provided only as general guidance, and individual patient results should be interpreted in light of clinical signs and symptoms.  Troughs should be drawn immediately before the next dose.

 

Before 05/20/2014:

For Q8 dosing, peak levels should be 5-10 ug/mL and trough levels should be less than 2 ug/mL.
For QD dosingpeak levels are not necessary the majority of time, but should be greater than 8 ug/mL. Trough levels should be less than 1 ug/mL, with the level indicating a potential adjustment in dose if greater than 2 ug/mL

Trough should be drawn immediately before the nest dose. Peaks should be drawn 30 minutes after the end of a 30-60 minute aminoglycosdie infusion.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

80200

Collection

LAB38

TOB T

Collect

One 4.5 mL Green top tube with gel separator (PST).

Pediatric Collection

1.5 mL whole blood or 20 full capillary tubes.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection. Send at 2-8 degrees C if tested within 24 hours. Place plasma in aliquot container and send frozen if not tested within 24 hours.

Stability (from collection to initiation)

Refrigerated: 24 hours; Frozen (-20 degreees C): Greater than 24 hours.

Remarks

Draw trough level immediately before the next dose.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Enzymatic

Reported

Same day

Synonyms

  • LAB38
Result Interpretation

Reference Interval

Effective 05/20/2014:

0 - 2 ug/mL

Interpretive Data

Effective 05/20/2014:

Reports show that in patients on divided daily dosage regimens, trough concentrations of  1–2  μg/mL  usually  ensure  that  the  concentration  is  above  the  MIC of most tobramycin-sensitive pathogens and that the drug elimination is adequate.  When patients are on a QD regimen, reports show that trough concentrations less than 1 μg/mL allow adequate clearance before the next dose.  Prolonged  trough  concentrations  above  2  μg/mL  are  often  associated  with  renal  impairment and ototoxicity.  For effective treatment, some patients may require serum or plasma levels outside these ranges. Therefore, the expected ranges are provided only as general guidance, and individual patient results should be interpreted in light of clinical signs and symptoms.  Troughs should be drawn immediately before the next dose.

 

Before 05/20/2014:

For Q8 dosing, peak levels should be 5-10 ug/mL and trough levels should be less than 2 ug/mL.
For QD dosingpeak levels are not necessary the majority of time, but should be greater than 8 ug/mL. Trough levels should be less than 1 ug/mL, with the level indicating a potential adjustment in dose if greater than 2 ug/mL

Trough should be drawn immediately before the nest dose. Peaks should be drawn 30 minutes after the end of a 30-60 minute aminoglycosdie infusion.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

80200

TOCAINIDE

LAB3096

Collect

red, gray (sodium fluoride/potassium oxalate), green (sodium heparin), or lavender (EDTA).

Specimen Preparation

Separate serum or plasma from cells ASAP. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)

Unacceptable Conditions

Separator tubes.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 4 months

Performed

Varies

Methodology

Gas Chromatography

Reported

3-10 days

Synonyms

  • Tonocard{R}
  • LAB3096
  • TOCAINIDE

Reference Interval

By report

CPT Codes

80375 (Alt code: G0480)
Collection

LAB3096

Collect

red, gray (sodium fluoride/potassium oxalate), green (sodium heparin), or lavender (EDTA).

Specimen Preparation

Separate serum or plasma from cells ASAP. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)

Unacceptable Conditions

Separator tubes.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 4 months
Ordering

Performed

Varies

Methodology

Gas Chromatography

Reported

3-10 days

Synonyms

  • Tonocard{R}
  • LAB3096
  • TOCAINIDE
Result Interpretation

Reference Interval

By report
Administrative

CPT Codes

80375 (Alt code: G0480)

TOPIRAMATE

LAB498

Collect

Plain red. Also acceptable: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Patient Preparation

Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration.

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Serum or plasma separator tubes.  Grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 6 days; Refrigerated: 1 week; Frozen: 4 weeks

Performed

Sun-Sat

Methodology

Quantitative Enzyme Immunoassay

Reported

Within 24 hours

Synonyms

  • Topamax
  • LAB498

Ordering Recommendations

Optimize drug therapy and monitor patient adherence.

Reference Interval

Effective November 18, 2013

Therapeutic range:  5.0-20.0 µg/mL
Toxic:                Not well established

Interpretive Data

Pharmacokinetics varies widely, particularly with co-medications, age, and/or compromised renal function. Adverse effects may include somnolence, fatigue, and dizziness.

CPT Codes

80201
Collection

LAB498

Collect

Plain red. Also acceptable: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Patient Preparation

Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration.

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Serum or plasma separator tubes.  Grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 6 days; Refrigerated: 1 week; Frozen: 4 weeks
Ordering

Performed

Sun-Sat

Methodology

Quantitative Enzyme Immunoassay

Reported

Within 24 hours

Synonyms

  • Topamax
  • LAB498

Ordering Recommendations

Optimize drug therapy and monitor patient adherence.
Result Interpretation

Reference Interval

Effective November 18, 2013

Therapeutic range:  5.0-20.0 µg/mL
Toxic:                Not well established

Interpretive Data

Pharmacokinetics varies widely, particularly with co-medications, age, and/or compromised renal function. Adverse effects may include somnolence, fatigue, and dizziness.

Administrative

CPT Codes

80201

TORCH IGG ANTIBODIES

LAB3209

Collect

Serum separator tube. Also acceptable: Serum from umbilical cord blood.

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Refer to individual components.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

Performed

Sun-Sat

Methodology

Semi-Quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • LAB3209
  • TORCH IGG

Ordering Recommendations

Not recommended for diagnosing congenital infections in newborns; tests should be selected individually to target the most likely infectious agents. May be used in pregnant women to assess past exposure or immunization to Toxoplasma, Rubella, CMV, and HSV.

Reference Interval

Components
Reference Interval
Cytomegalovirus Antibody, IgG
0.59 U/mL or less:Not Detected.
0.60-0.69 U/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
0.70 U/mL or greater:Detected.

Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG
0.89 IV or less:Not Detected.
0.90-1.09 IV:Indeterminate - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater:Detected.

Rubella Antibody, IgG
Less than 9 IU/mL:Not Detected.
9-9.9 IU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
10 IU/mL or greater:Detected.

Toxoplasma gondii Antibody, IgGEffective March 3, 2014
7.1 IU/mL or less:Not Detected.
7.2-8.7 IU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
8.8 IU/mL or greater:Detected.



Interpretive Data

Refer to report.

CPT Codes

86644; 86694; 86762; 86777
Collection

LAB3209

Collect

Serum separator tube. Also acceptable: Serum from umbilical cord blood.

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Refer to individual components.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).
Ordering

Performed

Sun-Sat

Methodology

Semi-Quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • LAB3209
  • TORCH IGG

Ordering Recommendations

Not recommended for diagnosing congenital infections in newborns; tests should be selected individually to target the most likely infectious agents. May be used in pregnant women to assess past exposure or immunization to Toxoplasma, Rubella, CMV, and HSV.
Result Interpretation

Reference Interval

Components
Reference Interval
Cytomegalovirus Antibody, IgG
0.59 U/mL or less:Not Detected.
0.60-0.69 U/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
0.70 U/mL or greater:Detected.

Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgG
0.89 IV or less:Not Detected.
0.90-1.09 IV:Indeterminate - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater:Detected.

Rubella Antibody, IgG
Less than 9 IU/mL:Not Detected.
9-9.9 IU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
10 IU/mL or greater:Detected.

Toxoplasma gondii Antibody, IgGEffective March 3, 2014
7.1 IU/mL or less:Not Detected.
7.2-8.7 IU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
8.8 IU/mL or greater:Detected.



Interpretive Data

Refer to report.

Administrative

CPT Codes

86644; 86694; 86762; 86777

TORCH IGM ANTIBODIES

LAB3210

Collect

Serum separator tube. Also acceptable: Serum from umbilical cord blood.

Specimen Preparation

Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Refer to individual components.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

Performed

Sun-Sat

Methodology

Semi-Quantitative Chemiluminescent Immunoassay/Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-2 days

Synonyms

  • LAB3210
  • TORCH IGM AN

Ordering Recommendations

Not recommended for diagnosing congenital infections in newborns; tests should be selected individually to target the most likely infectious agents.

Reference Interval

Components
Reference Interval
Cytomegalovirus Antibody, IgM
29.9 AU/mL or less:Not Detected.
30.0-34.9 AU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
35.0 AU/mL or greater:Detected - IgM antibody to CMV detected, which may indicate a current or recent infection.  However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgM by ELISA
0.89 IV or less:Not Detected.
0.90-1.09 IV:Indeterminate - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater:Detected - IgM antibody to HSV detected, which may indicate a current or recent infection. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Rubella Antibody, IgM
19.9 AU/mL or less:Not Detected.
20.0 - 24.9 AU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
25.0 AU/mL or greater:Detected - IgM antibody to rubella detected, which may indicate a current or recent infection or immunization.

Toxoplasma gondii Antibody, IgM
7.9 AU/mL or less:Not Detected.
8.0-9.9 AU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
10.0 AU/mL or greater:Detected - Significant level of Toxoplasma gondii IgM antibody detected and may indicate a current or recent infection. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.



Interpretive Data

Refer to report.

CPT Codes

86645; 86694; 86762; 86778
Collection

LAB3210

Collect

Serum separator tube. Also acceptable: Serum from umbilical cord blood.

Specimen Preparation

Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Refer to individual components.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).
Ordering

Performed

Sun-Sat

Methodology

Semi-Quantitative Chemiluminescent Immunoassay/Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-2 days

Synonyms

  • LAB3210
  • TORCH IGM AN

Ordering Recommendations

Not recommended for diagnosing congenital infections in newborns; tests should be selected individually to target the most likely infectious agents.
Result Interpretation

Reference Interval

Components
Reference Interval
Cytomegalovirus Antibody, IgM
29.9 AU/mL or less:Not Detected.
30.0-34.9 AU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
35.0 AU/mL or greater:Detected - IgM antibody to CMV detected, which may indicate a current or recent infection.  However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgM by ELISA
0.89 IV or less:Not Detected.
0.90-1.09 IV:Indeterminate - Repeat testing in 10-14 days may be helpful.
1.10 IV or greater:Detected - IgM antibody to HSV detected, which may indicate a current or recent infection. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Rubella Antibody, IgM
19.9 AU/mL or less:Not Detected.
20.0 - 24.9 AU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
25.0 AU/mL or greater:Detected - IgM antibody to rubella detected, which may indicate a current or recent infection or immunization.

Toxoplasma gondii Antibody, IgM
7.9 AU/mL or less:Not Detected.
8.0-9.9 AU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
10.0 AU/mL or greater:Detected - Significant level of Toxoplasma gondii IgM antibody detected and may indicate a current or recent infection. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.



Interpretive Data

Refer to report.

Administrative

CPT Codes

86645; 86694; 86762; 86778

TOTAL BILIRUBIN BODY FLUID

LAB182

BILI T BF

Collect

1 mL fluid in sterile container.

Pediatric Collection

0.2 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport at ambient temperature.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: 7 days (protected from light).

Remarks

Identify fluid source on requisition. Protect from light.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Jendrassik-Grof

Reported

Same day

Synonyms

  • LAB182
  • BILI T BF

Interpretive Data

The reference range for bilirubin in body fluids is undefined.  The result must be integrated into the clinical context and/or compared to a synchronous serum or plasma measurement. 
This test was developed and its performance characteristics determined by University of Colorado Hospital.  It has not been cleared or approved by the FDA.  The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

82247

Collection

LAB182

BILI T BF

Collect

1 mL fluid in sterile container.

Pediatric Collection

0.2 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport at ambient temperature.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: 7 days (protected from light).

Remarks

Identify fluid source on requisition. Protect from light.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Jendrassik-Grof

Reported

Same day

Synonyms

  • LAB182
  • BILI T BF
Result Interpretation

Interpretive Data

The reference range for bilirubin in body fluids is undefined.  The result must be integrated into the clinical context and/or compared to a synchronous serum or plasma measurement. 
This test was developed and its performance characteristics determined by University of Colorado Hospital.  It has not been cleared or approved by the FDA.  The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

82247

TOTAL COMPLEMENT--CH50

LAB154

Collect

Plain red.

Specimen Preparation

Allow specimen to clot for one hour at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Separator tubes. Specimens left to clot at 2-8°C. Specimens exposed to repeated freeze/thaw cycles. Non-frozen specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

After separation from cells: Ambient: 2 hours; Refrigerated: Unacceptable; Frozen: 2 weeks

Performed

Mon-Fri

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-2 days

Synonyms

  • CH50
  • Complement CH100, Total
  • Total Complement CH50
  • Total Complement CH100
  • CH100
  • LAB154

Additional Technical Information

Ordering Recommendations

Initial screening for suspected deficiency in the classical complement pathway.

Reference Interval

Effective May 16, 2011

60-144 CAE Units

CPT Codes

86162
Collection

LAB154

Collect

Plain red.

Specimen Preparation

Allow specimen to clot for one hour at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Separator tubes. Specimens left to clot at 2-8°C. Specimens exposed to repeated freeze/thaw cycles. Non-frozen specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

After separation from cells: Ambient: 2 hours; Refrigerated: Unacceptable; Frozen: 2 weeks
Ordering

Performed

Mon-Fri

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-2 days

Synonyms

  • CH50
  • Complement CH100, Total
  • Total Complement CH50
  • Total Complement CH100
  • CH100
  • LAB154

Additional Technical Information

Ordering Recommendations

Initial screening for suspected deficiency in the classical complement pathway.
Result Interpretation

Reference Interval

Effective May 16, 2011

60-144 CAE Units
Administrative

CPT Codes

86162

TOTAL NITROGEN URINE

LAB3562 - Order as Misc Test

 

Collect

24-hour urine.

Specimen Preparation

Transfer 10 mL aliquot from a well-mixed 24-hour urine collection to ARUP Standard Transport Tubes. (Min: 2 mL) Do not use preservatives.

Storage/Transport Temperature

Refrigerated. Also acceptable: Room temperature or frozen.

Stability (from collection to initiation)

Ambient: 1 week; Refrigerated: 1 week; Frozen: 3 years

Remarks

24 hour volume required.

Performed

Varies

Methodology

Quantitative Dumas Combustion

Reported

4-11 days

Synonyms

  • URINE TOTAL NITROGEN
  • NITROGEN, TOTAL, TIMED URINE
  • LAB449

Reference Interval

By report

CPT Codes

84999
Collection

LAB3562 - Order as Misc Test

 

Collect

24-hour urine.

Specimen Preparation

Transfer 10 mL aliquot from a well-mixed 24-hour urine collection to ARUP Standard Transport Tubes. (Min: 2 mL) Do not use preservatives.

Storage/Transport Temperature

Refrigerated. Also acceptable: Room temperature or frozen.

Stability (from collection to initiation)

Ambient: 1 week; Refrigerated: 1 week; Frozen: 3 years

Remarks

24 hour volume required.
Ordering

Performed

Varies

Methodology

Quantitative Dumas Combustion

Reported

4-11 days

Synonyms

  • URINE TOTAL NITROGEN
  • NITROGEN, TOTAL, TIMED URINE
  • LAB449
Result Interpretation

Reference Interval

By report
Administrative

CPT Codes

84999

TOTAL PROTEIN, DIALYSATE

LAB194

PROT DIASYL

Collect

1 mL fluid in sterile container.

Pediatric Collection

0.2 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport refrigerated.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: 3 days; Frozen: 6 months.

Remarks

Identify fluid source on requisition.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Spectrophotometric

Reported

Same day

Synonyms

  • LAB194

Performed by

PCL Clinical Laboratory - Core

Collection

LAB194

PROT DIASYL

Collect

1 mL fluid in sterile container.

Pediatric Collection

0.2 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport refrigerated.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: 3 days; Frozen: 6 months.

Remarks

Identify fluid source on requisition.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Spectrophotometric

Reported

Same day

Synonyms

  • LAB194
Result Interpretation

Performed by

PCL Clinical Laboratory - Core

Administrative

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