Tests Catalogs

TRYPTASE TOTAL & MATURE

LAB827

Collect

7 mL red top or 6 mL gold with gel

Unacceptable Conditions

Non-frozen samples.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 3 ml Red Top Centrifuge specimen and pour-off serum, refrigerate or freeze the serum and deliver to the lab. Minimum volume = 1.0 mL serum.

Stability (from collection to initiation)

Refrigerated: 8 hours. Frozen:  3-4 weeks

Remarks

Result includes mature [a & B] tryptase level and total UniCap tryptase level.

Performed by

Virginia Commonwealth University

Performed

Thurs

Methodology

FEIA Enzyme Immunoassay w/Fluorescent substrate

Reported

Varies

Synonyms

  • LAB827

Performed by

Virginia Commonwealth University

CPT Codes

83520

Collection

LAB827

Collect

7 mL red top or 6 mL gold with gel

Unacceptable Conditions

Non-frozen samples.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 3 ml Red Top Centrifuge specimen and pour-off serum, refrigerate or freeze the serum and deliver to the lab. Minimum volume = 1.0 mL serum.

Stability (from collection to initiation)

Refrigerated: 8 hours. Frozen:  3-4 weeks

Remarks

Result includes mature [a & B] tryptase level and total UniCap tryptase level.

Performed by

Virginia Commonwealth University

Ordering

Performed

Thurs

Methodology

FEIA Enzyme Immunoassay w/Fluorescent substrate

Reported

Varies

Synonyms

  • LAB827
Result Interpretation

Performed by

Virginia Commonwealth University

Administrative

CPT Codes

83520

TSH RECEPTOR ANTIBODY [TRAB]

LAB574

Collect

Serum separator tube.

Specimen Preparation

Allow serum separator to sit for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Plasma. Grossly hemolyzed or lipemic specimens.

Storage/Transport Temperature

Frozen. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 72 hours; Frozen: 1 month

Performed

Sun-Sat

Methodology

Quantitative Electrochemiluminescent Immunoassay

Reported

1-2 days

Synonyms

  • TRAB 3rd Generation
  • Third Generation TRAb
  • Thyroid Stimulation Hormone Receptor Antibody (TRAb) Ultrasensitive
  • LAB574

Additional Technical Information

Ordering Recommendations

Acceptable testing for autoimmune thyroid disease.

Reference Interval

Less than or equal to 1.75 IU/L

Interpretive Data

Autoimmune thyroid disease may be confirmed when TRAb testing is positive.

CPT Codes

83520
Collection

LAB574

Collect

Serum separator tube.

Specimen Preparation

Allow serum separator to sit for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Plasma. Grossly hemolyzed or lipemic specimens.

Storage/Transport Temperature

Frozen. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 72 hours; Frozen: 1 month
Ordering

Performed

Sun-Sat

Methodology

Quantitative Electrochemiluminescent Immunoassay

Reported

1-2 days

Synonyms

  • TRAB 3rd Generation
  • Third Generation TRAb
  • Thyroid Stimulation Hormone Receptor Antibody (TRAb) Ultrasensitive
  • LAB574

Additional Technical Information

Ordering Recommendations

Acceptable testing for autoimmune thyroid disease.
Result Interpretation

Reference Interval

Less than or equal to 1.75 IU/L

Interpretive Data

Autoimmune thyroid disease may be confirmed when TRAb testing is positive.

Administrative

CPT Codes

83520

TSH ULTRASENSITIVE

LAB129

TSH

Collect

One 4.5 mL green top with gel (PST), (lithium heparin plasma).

Pediatric Collection

At least 0.5 mL whole blood in green top microtainer with gel, (lithium heparin plasma) or other heparin tube.

Unacceptable Conditions

Grossly hemolyzed samples; EDTA anticoagulant; sample not properly identified; insufficient sample volume; improperly stored.

 

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection.  If delivered to lab within 4 hours, transport to lab at 2-8 degrees C. If delivered to lab in more than 4 hours, place at least 1.0 mL plasma in aliquot container and transport to the lab frozen.

Stability (from collection to initiation)

Before centrifugation: 1 hour.

After centrifugation: Ambient, 2 hours; Refrigerated, 4 hours.

 

Plasma aliquot: Ambient, 8 hours; Refrigerated, 48 hours; Frozen (-20 degrees C), 1 month.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Chemiluminescent EIA

Reported

Daily

Synonyms

  • LAB129
  • THYROID STIMULATING HORMONE

Reference Interval

0.34 - 5.60 mIU/L

Interpretive Data

Testing is performed by Access HYPERsensitive hTSH (3rd generation).  NHANES III data suggests that TSH levels for thyroid disease-free adults are generally between 0.45 and 4.12 mIU/L and are age-dependent.  Reference intervals for pregnant patients and neonates have not been validated.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84443

Collection

LAB129

TSH

Collect

One 4.5 mL green top with gel (PST), (lithium heparin plasma).

Pediatric Collection

At least 0.5 mL whole blood in green top microtainer with gel, (lithium heparin plasma) or other heparin tube.

Unacceptable Conditions

Grossly hemolyzed samples; EDTA anticoagulant; sample not properly identified; insufficient sample volume; improperly stored.

 

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection.  If delivered to lab within 4 hours, transport to lab at 2-8 degrees C. If delivered to lab in more than 4 hours, place at least 1.0 mL plasma in aliquot container and transport to the lab frozen.

Stability (from collection to initiation)

Before centrifugation: 1 hour.

After centrifugation: Ambient, 2 hours; Refrigerated, 4 hours.

 

Plasma aliquot: Ambient, 8 hours; Refrigerated, 48 hours; Frozen (-20 degrees C), 1 month.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Chemiluminescent EIA

Reported

Daily

Synonyms

  • LAB129
  • THYROID STIMULATING HORMONE
Result Interpretation

Reference Interval

0.34 - 5.60 mIU/L

Interpretive Data

Testing is performed by Access HYPERsensitive hTSH (3rd generation).  NHANES III data suggests that TSH levels for thyroid disease-free adults are generally between 0.45 and 4.12 mIU/L and are age-dependent.  Reference intervals for pregnant patients and neonates have not been validated.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84443

TSPOT

LAB4081

Collect

10 mL whole blood green top tube (sodium heparin). Samples must arrive within 24 hours of draw and are accepted Monday - Noon on Thursday. **SAMPLES ARE NOT ACCEPTED ON FRIDAY**

Pediatric Collection

6 mL whole blood Green top tube (sodium heparin).

Unacceptable Conditions

Frozen, clotted, hemolyzed, spun down, citrate anticoagulant (blue top).

Storage/Transport Temperature

Room temperature.

Stability (from collection to initiation)

24 hours at room temperature.

Remarks

Quantiferon must be an indeterminate result.

Performed by

National Jewish

Synonyms

  • TUBERCULOSIS INTERFERON GAMMA RELEASE ASSAY

Performed by

National Jewish

Collection

LAB4081

Collect

10 mL whole blood green top tube (sodium heparin). Samples must arrive within 24 hours of draw and are accepted Monday - Noon on Thursday. **SAMPLES ARE NOT ACCEPTED ON FRIDAY**

Pediatric Collection

6 mL whole blood Green top tube (sodium heparin).

Unacceptable Conditions

Frozen, clotted, hemolyzed, spun down, citrate anticoagulant (blue top).

Storage/Transport Temperature

Room temperature.

Stability (from collection to initiation)

24 hours at room temperature.

Remarks

Quantiferon must be an indeterminate result.

Performed by

National Jewish

Ordering

Synonyms

  • TUBERCULOSIS INTERFERON GAMMA RELEASE ASSAY
Result Interpretation

Performed by

National Jewish

Administrative

TSPOT for INDETERMINATE QFG PATIENTS

LAB4081

Collect

2 Dark Green top tubes (10 mL preferred)

Minimum volume:  6 mL

Samples must arrive at the testing laboratory within 24 hours of collection and should be collected Monday thru NOON on Thursday. Samples are NOT acceptable on Fridays.

This test should only be ordered on immunocompromised patients with previously INDETERMINATE Qunatiferon Gold test results.

 

Specimen Preparation

Do NOT Centrifuge. Store/transport at room temperature.

Unacceptable Conditions

Samples received at the testing laboratory after 1200 on Thursdays or on Fridays.

Frozen, clotted, hemolyzed, spun down, citrated anticoagulant.

Storage/Transport Temperature

Maintain and transport specimens at room temperature (18-22 C).

 

Stability (from collection to initiation)

24 hours at room temperature.

Performed by

National Jewish Health Advanced Diagnostic Laboratories

Performed

Monday-Thursday

Methodology

Elispot

Reported

In 48 hours.

Ordering Recommendations

This test should only be ordered on immunocompromised patients with previously INDETERMINATE Qunatiferon Gold test results.

Reference Interval

Negative

Interpretive Data

The T-SPOT.TB test is based on the measurement of a cell mediated response to Mycobacterium tuberculosis-specific antigens (ESAT-6 and CFP-10). Peripheral blood mononuclear cells (PBMC) are separated from whole blood and incubated in plates coated with antiinterferongamma (IFNg) antibodies with these antigens for 16-24 hours. Following incubation, IFNg-producing cells are detected usinga second anti- IFNg antibody, an enzyme linked conjugate, and substrate. Controls include unstimulated cells (Nil control) andphytohemagglutinin stimulated cells (Mitogen control). A test is considered positive or borderline when IFNg- spot forming units (SFU) produced in response to either of the TB antigens is significantly greater than the Nil control (TB-Nil; positive = > 8 SFU, borderline = 5, 6 or 7 SFU), and the Mitogen control has a robust IFNg response. The T-Spot.TB result is indeterminate if the Nil control is high (>10 SFU) or the mitogen control is low (< 20 SFU). Although the test is reported qualitatively, IFNg SFU for the individual test c

1.The T-Spot.TB detects infections due to M. tuberculosis complex (M. tuberculosis, M. bovis and M. africanum). BCG strains and

nontuberculous mycobacteria with the exception of M. marinum, M. kansasii, M. szulgai and M. gordonae do not express ESAT-6 and CFP-10 proteins. Therefore, patients either vaccinated with BCG or infected with most nontuberculous mycobacterial strains should test negative.

T-Spot.TB results should always be interpreted in conjunction with clinical and other relevant laboratory findings.

1 Guidelines for Using the QuantiFERON®-TB Gold Test for Detecting Mycobacterium tuberculosis Infection, United States. Centers for Disease Control and Prevention. Morbidity and Mortality Weekly Report (MMWR). December 16, 2005 / 54(RR15);49-55

onditions are also reported as recommended by the Centers for Disease Control and Prevention.

 

Performed by

National Jewish Health Advanced Diagnostic Laboratories

CPT Codes

86481

Collection

LAB4081

Collect

2 Dark Green top tubes (10 mL preferred)

Minimum volume:  6 mL

Samples must arrive at the testing laboratory within 24 hours of collection and should be collected Monday thru NOON on Thursday. Samples are NOT acceptable on Fridays.

This test should only be ordered on immunocompromised patients with previously INDETERMINATE Qunatiferon Gold test results.

 

Specimen Preparation

Do NOT Centrifuge. Store/transport at room temperature.

Unacceptable Conditions

Samples received at the testing laboratory after 1200 on Thursdays or on Fridays.

Frozen, clotted, hemolyzed, spun down, citrated anticoagulant.

Storage/Transport Temperature

Maintain and transport specimens at room temperature (18-22 C).

 

Stability (from collection to initiation)

24 hours at room temperature.

Performed by

National Jewish Health Advanced Diagnostic Laboratories

Ordering

Performed

Monday-Thursday

Methodology

Elispot

Reported

In 48 hours.

Ordering Recommendations

This test should only be ordered on immunocompromised patients with previously INDETERMINATE Qunatiferon Gold test results.

Result Interpretation

Reference Interval

Negative

Interpretive Data

The T-SPOT.TB test is based on the measurement of a cell mediated response to Mycobacterium tuberculosis-specific antigens (ESAT-6 and CFP-10). Peripheral blood mononuclear cells (PBMC) are separated from whole blood and incubated in plates coated with antiinterferongamma (IFNg) antibodies with these antigens for 16-24 hours. Following incubation, IFNg-producing cells are detected usinga second anti- IFNg antibody, an enzyme linked conjugate, and substrate. Controls include unstimulated cells (Nil control) andphytohemagglutinin stimulated cells (Mitogen control). A test is considered positive or borderline when IFNg- spot forming units (SFU) produced in response to either of the TB antigens is significantly greater than the Nil control (TB-Nil; positive = > 8 SFU, borderline = 5, 6 or 7 SFU), and the Mitogen control has a robust IFNg response. The T-Spot.TB result is indeterminate if the Nil control is high (>10 SFU) or the mitogen control is low (< 20 SFU). Although the test is reported qualitatively, IFNg SFU for the individual test c

1.The T-Spot.TB detects infections due to M. tuberculosis complex (M. tuberculosis, M. bovis and M. africanum). BCG strains and

nontuberculous mycobacteria with the exception of M. marinum, M. kansasii, M. szulgai and M. gordonae do not express ESAT-6 and CFP-10 proteins. Therefore, patients either vaccinated with BCG or infected with most nontuberculous mycobacterial strains should test negative.

T-Spot.TB results should always be interpreted in conjunction with clinical and other relevant laboratory findings.

1 Guidelines for Using the QuantiFERON®-TB Gold Test for Detecting Mycobacterium tuberculosis Infection, United States. Centers for Disease Control and Prevention. Morbidity and Mortality Weekly Report (MMWR). December 16, 2005 / 54(RR15);49-55

onditions are also reported as recommended by the Centers for Disease Control and Prevention.

 

Performed by

National Jewish Health Advanced Diagnostic Laboratories

Administrative

CPT Codes

86481

TULAREMIA AB - FRANCISELLA TULARENSIS ANTIBODIES IGG/IGM

Collect

Serum separator tube (SST), lavender (EDTA), Lt. blue (sodium citrate) or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Unacceptable Conditions

Contaminated, heat-inactivated, or turbid specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Performed

Mon, Wed, Fri

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-5 days

Synonyms

  • Febrile Antigens (Francisella tularensis Antibodies, IgG and IgM)

Ordering Recommendations

Preferred test for detecting antibodies during acute or convalescent phase. Convalescent sera may be required for diagnosis.

Reference Interval

Components
Reference Interval
Francisella tularensis Antibody, IgGEffective May 9, 2011
9 U/mL or lessNegative - No significant level of IgG antibody to Francisella tularensis detected.
10-15 U/mLEquivocal - Questionable presence of IgG antibody to Francisella tularensis. Repeat testing in 10-14 days may be helpful.
16 U/mL or greaterPositive - Presence of IgG antibody to Francisella tularensis detected, suggestive of current or past exposure/immunization.

Francisella tularensis Antibody, IgMEffective May 9, 2011
9 U/mL or lessNegative - No significant level of IgM antibody to Francisella tularensis detected.
10-15 U/mLEquivocal - Questionable presence of IgM antibody to Francisella tularensis. Repeat testing in 10-14 days may be helpful.
16 U/mL or greaterPositive - Presence of IgM antibody to Francisella tularensis detected, suggestive of current or recent exposure/immunization.


Interpretive Data

Cross reactivity with Brucella and Yersinia antibodies may occur. Therefore, results should be interpreted with caution and correlated with clinical information. The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are performed in the same laboratory at the same time.

CPT Codes

86668 x2
Collection

Collect

Serum separator tube (SST), lavender (EDTA), Lt. blue (sodium citrate) or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Unacceptable Conditions

Contaminated, heat-inactivated, or turbid specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
Ordering

Performed

Mon, Wed, Fri

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-5 days

Synonyms

  • Febrile Antigens (Francisella tularensis Antibodies, IgG and IgM)

Ordering Recommendations

Preferred test for detecting antibodies during acute or convalescent phase. Convalescent sera may be required for diagnosis.
Result Interpretation

Reference Interval

Components
Reference Interval
Francisella tularensis Antibody, IgGEffective May 9, 2011
9 U/mL or lessNegative - No significant level of IgG antibody to Francisella tularensis detected.
10-15 U/mLEquivocal - Questionable presence of IgG antibody to Francisella tularensis. Repeat testing in 10-14 days may be helpful.
16 U/mL or greaterPositive - Presence of IgG antibody to Francisella tularensis detected, suggestive of current or past exposure/immunization.

Francisella tularensis Antibody, IgMEffective May 9, 2011
9 U/mL or lessNegative - No significant level of IgM antibody to Francisella tularensis detected.
10-15 U/mLEquivocal - Questionable presence of IgM antibody to Francisella tularensis. Repeat testing in 10-14 days may be helpful.
16 U/mL or greaterPositive - Presence of IgM antibody to Francisella tularensis detected, suggestive of current or recent exposure/immunization.


Interpretive Data

Cross reactivity with Brucella and Yersinia antibodies may occur. Therefore, results should be interpreted with caution and correlated with clinical information. The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are performed in the same laboratory at the same time.

Administrative

CPT Codes

86668 x2

TUMOR MARKER - BHCG

LAB3958

Collect

Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.4 mL)

Unacceptable Conditions

CSF (refer to Beta-hCG, Quantitative (Tumor Marker) CSF, ARUP test code 0020730). Specimens left to clot at 2-8°C or specimens subjected to repeated freeze/thaw cycles.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 5 days; Refrigerated: 2 weeks; Frozen: 1 year

Performed

Sun-Sat

Methodology

Quantitative Electrochemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • LAB3958
  • BHCG TM

Reference Interval

Male: 0-3 IU/L
Female: 0-5 IU/L

Interpretive Data

Interpretive Data: Human chorionic gonadotropin (hCG) is a valuable aid in the management of patients with trophoblastic tumors, nonseminomatous testicular tumors, and seminomas when used in conjunction with information available from the clinical evaluation and other diagnostic procedures. Increased serum hCG concentrations have also been observed in melanoma, carcinomas of the breast, gastrointestinal tract, lung, and ovaries, and in benign conditions, including cirrhosis, duodenal ulcer, and inflammatory bowel disease. This result cannot be interpreted as absolute evidence of the presence or absence of malignant disease. This result is not interpretable as a tumor marker in pregnant females.

The combination of the specific monoclonal antibodies used in the Roche Beta HCG electrochemiluminescent immunoassay recognize the holo-hormone, "nicked" forms of hCG, the beta-core fragment, and the free beta-subunit. Results obtained with different test methods or kits cannot be used interchangeably. Although this assay is FDA cleared for use in the detection of pregnancy, it is not labeled for use as a tumor marker.

CPT Codes

84702
Collection

LAB3958

Collect

Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.4 mL)

Unacceptable Conditions

CSF (refer to Beta-hCG, Quantitative (Tumor Marker) CSF, ARUP test code 0020730). Specimens left to clot at 2-8°C or specimens subjected to repeated freeze/thaw cycles.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 5 days; Refrigerated: 2 weeks; Frozen: 1 year
Ordering

Performed

Sun-Sat

Methodology

Quantitative Electrochemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • LAB3958
  • BHCG TM
Result Interpretation

Reference Interval

Male: 0-3 IU/L
Female: 0-5 IU/L

Interpretive Data

Interpretive Data: Human chorionic gonadotropin (hCG) is a valuable aid in the management of patients with trophoblastic tumors, nonseminomatous testicular tumors, and seminomas when used in conjunction with information available from the clinical evaluation and other diagnostic procedures. Increased serum hCG concentrations have also been observed in melanoma, carcinomas of the breast, gastrointestinal tract, lung, and ovaries, and in benign conditions, including cirrhosis, duodenal ulcer, and inflammatory bowel disease. This result cannot be interpreted as absolute evidence of the presence or absence of malignant disease. This result is not interpretable as a tumor marker in pregnant females.

The combination of the specific monoclonal antibodies used in the Roche Beta HCG electrochemiluminescent immunoassay recognize the holo-hormone, "nicked" forms of hCG, the beta-core fragment, and the free beta-subunit. Results obtained with different test methods or kits cannot be used interchangeably. Although this assay is FDA cleared for use in the detection of pregnancy, it is not labeled for use as a tumor marker.

Administrative

CPT Codes

84702

TUMOR NECROSIS FACTOR ALPHA

LAB3196

Collect

Serum separator tube, plain red, or green (lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Refrigerated specimens. Contaminated or heat-inactivated specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Additional specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

After separation from cells: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: 1 year

Notes

Lower limit of detection is 5 pg/mL.

Performed

Mon, Wed, Fri

Methodology

Quantitative Multiplex Bead Assay

Reported

1-4 days

Synonyms

  • LAB3196
  • TNFA

Reference Interval

Effective May 19, 2014
22 pg/mL or less

Interpretive Data

Results are used to understand the pathophysiology of immune, infectious, or inflammatory disorders, or may be used for research purposes.

CPT Codes

83520
Collection

LAB3196

Collect

Serum separator tube, plain red, or green (lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Refrigerated specimens. Contaminated or heat-inactivated specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Additional specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

After separation from cells: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: 1 year

Notes

Lower limit of detection is 5 pg/mL.
Ordering

Performed

Mon, Wed, Fri

Methodology

Quantitative Multiplex Bead Assay

Reported

1-4 days

Synonyms

  • LAB3196
  • TNFA
Result Interpretation

Reference Interval

Effective May 19, 2014
22 pg/mL or less

Interpretive Data

Results are used to understand the pathophysiology of immune, infectious, or inflammatory disorders, or may be used for research purposes.

Administrative

CPT Codes

83520

TYPE AND CROSSMATCH

Collect

One 6.0 mL Pink top (EDTA) labeled with  patient's last name, first name, medical record number, date drawn, phlebotomist's initials, and second person verifier's initials.

Utilizing READBACK VERIFICATION, the phlebotomist and a second hospital personnel verify that the patient's identifiers on his/her hospital identification bracelet match those on the blood specimen. Both persons must initial the pink top tube and, if used, the pink Transfusion Service request form.

In the outpatient setting, it is permissible for the patient to verify his/her identification.

Tube must be filled to at least 80% capacity.

Pediatric Collection

Minimum volume: 3 mL whole blood.

Unacceptable Conditions

Specimen not properly identified/labeled; incorrect container; serum separator tubes; insufficient sample volume; specimen grossly hemolyzed.

Storage/Transport Temperature

Internal: Deliver to Blood Bank immediately at ambient temperature.
Offsite: Transport to laboratory on refrigerated cold pack within 24 hours. Do not allow to freeze.

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 72 hours, Frozen: Unacceptable..

Remarks

Blood is held for 3 days. The sample used for crossmatching must be no older than 3 days of age at the time of transfusion for patients who have been pregnant or transfused within the last 3 months or when the history is unknown.

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

Notes

Click herefor important information on the use of human blood and blood components.

Click here for PCL Policy/Procedure "Administration of Blood Components".

Forms requesting blood/components and forms accompanying blood samples from the recipient must contain sufficient information for positive identification of the recipient.

Joint Commission requires that the upper half of the Transfusion Service form be completed. This area requires the person requesting the blood or blood product transfusion and type and screen to have a justification of why the blood and/or products are being requested (check mark on the appropriate box on upper left side of the form).

This request form is considered a prescription for blood and/or blood products; to comply with the FDA, the name of both the ordering physician and the attending physician must be written on the form.

Before a specimen is used for blood grouping, typing, or compatibility testing, a qualified person in the Transfusion Service confirms that all identifying information on the request form is in agreement with that on the specimen label. If the specimen is mislabeled or unlabeled, a new specimen must be drawn.

Performed

Daily

Methodology

Hemagglutination.

Reported

Daily

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

CPT Codes

86850; 86900; 86901; 86920

Collection

Collect

One 6.0 mL Pink top (EDTA) labeled with  patient's last name, first name, medical record number, date drawn, phlebotomist's initials, and second person verifier's initials.

Utilizing READBACK VERIFICATION, the phlebotomist and a second hospital personnel verify that the patient's identifiers on his/her hospital identification bracelet match those on the blood specimen. Both persons must initial the pink top tube and, if used, the pink Transfusion Service request form.

In the outpatient setting, it is permissible for the patient to verify his/her identification.

Tube must be filled to at least 80% capacity.

Pediatric Collection

Minimum volume: 3 mL whole blood.

Unacceptable Conditions

Specimen not properly identified/labeled; incorrect container; serum separator tubes; insufficient sample volume; specimen grossly hemolyzed.

Storage/Transport Temperature

Internal: Deliver to Blood Bank immediately at ambient temperature.
Offsite: Transport to laboratory on refrigerated cold pack within 24 hours. Do not allow to freeze.

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 72 hours, Frozen: Unacceptable..

Remarks

Blood is held for 3 days. The sample used for crossmatching must be no older than 3 days of age at the time of transfusion for patients who have been pregnant or transfused within the last 3 months or when the history is unknown.

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

Notes

Click herefor important information on the use of human blood and blood components.

Click here for PCL Policy/Procedure "Administration of Blood Components".

Forms requesting blood/components and forms accompanying blood samples from the recipient must contain sufficient information for positive identification of the recipient.

Joint Commission requires that the upper half of the Transfusion Service form be completed. This area requires the person requesting the blood or blood product transfusion and type and screen to have a justification of why the blood and/or products are being requested (check mark on the appropriate box on upper left side of the form).

This request form is considered a prescription for blood and/or blood products; to comply with the FDA, the name of both the ordering physician and the attending physician must be written on the form.

Before a specimen is used for blood grouping, typing, or compatibility testing, a qualified person in the Transfusion Service confirms that all identifying information on the request form is in agreement with that on the specimen label. If the specimen is mislabeled or unlabeled, a new specimen must be drawn.

Ordering

Performed

Daily

Methodology

Hemagglutination.

Reported

Daily

Result Interpretation

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

Administrative

CPT Codes

86850; 86900; 86901; 86920

TYPE AND SCREEN

Collect

One 6.0 mL Pink top (EDTA) labeled with  patient's last name, first name, medical record number, date drawn, phlebotomist's initials, and second person verifier's initials.

Utilizing READBACK VERIFICATION, the phlebotomist and a second hospital personnel verify that the patient's identifiers on his/her hospital identification bracelet match those on the blood specimen. Both persons must initial the pink top tube and, if used, the pink Transfusion Service request form.

In the outpatient setting, it is permissible for the patient to verify his/her identification.

Also acceptable: One 4 mL Purple top (EDTA) labeled as above.

Tube must be filled to at least 80% capacity.

Pediatric Collection

Minimum volume: 3 mL whole blood.

Unacceptable Conditions

Specimen not properly identified/labeled; incorrect container; insufficient sample volume; specimen grossly hemolyzed.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Transport to Blood Bank at ambient temperature within 8 hours, or at 2-8 degrees C within 24 hours.

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 72 hours.

Pre-procedure specimens may be refrigerated up to 7 days for patients who have not been transfused and not been pregnant in the preceding three months.

Remarks

Test is ordered when anticipated blood usage is unlikely. Order Type and Crossmatch if transfusion is needed. If blood should be needed for transfusion subsequent to performance of type and screen, fax a requisition to the Blood Bank x84407. The crossmatch will then be performed.

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

Notes

Forms requesting blood/components and forms accompanying blood samples from the recipient must contain sufficient information for positive identification of the recipient.

Joint Commission requires that the upper half of the Transfusion Service form be completed. This area requires the person requesting the blood or blood product transfusion and type and screen to have a justification of why the blood and/or products are being requested (check mark on the appropriate box on upper left side of the form).

This request form is considered a prescription for blood and/or blood products; to comply with the FDA, the name of both the ordering physician and the attending physician must be written on the form.

Before a specimen is used for blood grouping, typing, or compatibility testing, a qualified person in the Transfusion Service confirms that all identifying information on the request form is in agreement with that on the specimen label. If the specimen is mislabeled or unlabeled, a new specimen must be drawn.

Performed

Daily

Methodology

Hemagglutination.

Reported

Daily

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

CPT Codes

86850; 86900; 86901
Collection

Collect

One 6.0 mL Pink top (EDTA) labeled with  patient's last name, first name, medical record number, date drawn, phlebotomist's initials, and second person verifier's initials.

Utilizing READBACK VERIFICATION, the phlebotomist and a second hospital personnel verify that the patient's identifiers on his/her hospital identification bracelet match those on the blood specimen. Both persons must initial the pink top tube and, if used, the pink Transfusion Service request form.

In the outpatient setting, it is permissible for the patient to verify his/her identification.

Also acceptable: One 4 mL Purple top (EDTA) labeled as above.

Tube must be filled to at least 80% capacity.

Pediatric Collection

Minimum volume: 3 mL whole blood.

Unacceptable Conditions

Specimen not properly identified/labeled; incorrect container; insufficient sample volume; specimen grossly hemolyzed.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Transport to Blood Bank at ambient temperature within 8 hours, or at 2-8 degrees C within 24 hours.

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 72 hours.

Pre-procedure specimens may be refrigerated up to 7 days for patients who have not been transfused and not been pregnant in the preceding three months.

Remarks

Test is ordered when anticipated blood usage is unlikely. Order Type and Crossmatch if transfusion is needed. If blood should be needed for transfusion subsequent to performance of type and screen, fax a requisition to the Blood Bank x84407. The crossmatch will then be performed.

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

Notes

Forms requesting blood/components and forms accompanying blood samples from the recipient must contain sufficient information for positive identification of the recipient.

Joint Commission requires that the upper half of the Transfusion Service form be completed. This area requires the person requesting the blood or blood product transfusion and type and screen to have a justification of why the blood and/or products are being requested (check mark on the appropriate box on upper left side of the form).

This request form is considered a prescription for blood and/or blood products; to comply with the FDA, the name of both the ordering physician and the attending physician must be written on the form.

Before a specimen is used for blood grouping, typing, or compatibility testing, a qualified person in the Transfusion Service confirms that all identifying information on the request form is in agreement with that on the specimen label. If the specimen is mislabeled or unlabeled, a new specimen must be drawn.

Ordering

Performed

Daily

Methodology

Hemagglutination.

Reported

Daily
Result Interpretation

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)
Administrative

CPT Codes

86850; 86900; 86901

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