TOXOCARA ANTIBODIES - IGG

LAB1232

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens.  Please mark specimen plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated or heat-inactivated specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Tue, Fri

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-5 days

Synonyms

  • LAB1232
  • TOXOCARA

Ordering Recommendations

Aid in the diagnosis of toxocariasis.

Reference Interval

0.299 OD or less: Negative - No significant level of Toxocara IgG antibody detected.
0.300-0.500 OD: Equivocal - Questionable presence of Toxocara IgG antibody detected. Repeat testing in 10-14 days may be helpful.
0.501 OD or greater: Positive - Presence of IgG antibody to Toxocara detected, suggestive of current or past infection.

CPT Codes

86682
Collection

LAB1232

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens.  Please mark specimen plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated or heat-inactivated specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Tue, Fri

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-5 days

Synonyms

  • LAB1232
  • TOXOCARA

Ordering Recommendations

Aid in the diagnosis of toxocariasis.
Result Interpretation

Reference Interval

0.299 OD or less: Negative - No significant level of Toxocara IgG antibody detected.
0.300-0.500 OD: Equivocal - Questionable presence of Toxocara IgG antibody detected. Repeat testing in 10-14 days may be helpful.
0.501 OD or greater: Positive - Presence of IgG antibody to Toxocara detected, suggestive of current or past infection.
Administrative

CPT Codes

86682

TOXOPLASMA ANTIBODIES IGG

Collect

Serum separator tube. Also acceptable: Serum from umbilical cord blood.

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Plasma or urine. Contaminated, heat-inactivated, or grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Sun-Sat

Methodology

Semi-quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Ordering Recommendations

Detect prior exposure to T. gondii.

Reference Interval

Effective March 3, 2014
7.1 IU/mL or less:Not Detected.
7.2-8.7 IU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
8.8 IU/mL or greater:Detected.

Interpretive Data

The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

The magnitude of the measured result is not indicative of the amount of antibody present.

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

CPT Codes

86777
Collection

Collect

Serum separator tube. Also acceptable: Serum from umbilical cord blood.

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Plasma or urine. Contaminated, heat-inactivated, or grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Sun-Sat

Methodology

Semi-quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Ordering Recommendations

Detect prior exposure to T. gondii.
Result Interpretation

Reference Interval

Effective March 3, 2014
7.1 IU/mL or less:Not Detected.
7.2-8.7 IU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
8.8 IU/mL or greater:Detected.

Interpretive Data

The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

The magnitude of the measured result is not indicative of the amount of antibody present.

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

Administrative

CPT Codes

86777

TOXOPLASMA ANTIBODIES IGM

LAB659

Collect

Serum separator tube. Also acceptable: Serum from umbilical cord blood.

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute" or "convalescent."

Unacceptable Conditions

Plasma or urine. Contaminated, heat-inactivated, or grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Sun-Sat

Methodology

Semi-Quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • LAB659

Ordering Recommendations

Detect presence of IgM antibodies to T. gondii. For male patients or nonpregnant female patients with equivocal or positive results, Toxoplasma gondii by PCR (0055591) may also be useful if a specimen can be collected from an affected body site.

Reference Interval

7.9 AU/mL or less:Not Detected.
8.0-9.9 AU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
10.0 AU/mL or greater:Detected - Significant level of Toxoplasma gondii IgM antibody detected and may indicate a current or recent infection. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Interpretive Data

This test is performed using the DiaSorin LIAISON. As suggested by the CDC, any indeterminate or detected Toxoplasma gondii IgM result should be retested in parallel with a specimen collected 1-3 weeks later. Further confirmation may be necessary using a different test from another reference laboratory specializing in toxoplasmosis testing where an IgM ELISA should be ordered. Caution should be exercised in the use of IgM antibody levels in prenatal screening. Any Toxoplasma gondii IgM in pregnant patients that have also been confirmed by a second reference laboratory should be evaluated by amniocentesis and PCR testing for Toxoplasma gondii.

For male and non-pregnant female patients with indeterminate or detected Toxoplasma gondii IgM results, PCR may also be useful if a specimen can be collected from an affected body site.

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

For additional information, refer to the CDC website: www.cdc.gov/parasites/toxoplasmosis/health_professionals/index.html.

The magnitude of the measured result is not indicative of the amount of antibody present.

CPT Codes

86778
Collection

LAB659

Collect

Serum separator tube. Also acceptable: Serum from umbilical cord blood.

Specimen Preparation

Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute" or "convalescent."

Unacceptable Conditions

Plasma or urine. Contaminated, heat-inactivated, or grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Sun-Sat

Methodology

Semi-Quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • LAB659

Ordering Recommendations

Detect presence of IgM antibodies to T. gondii. For male patients or nonpregnant female patients with equivocal or positive results, Toxoplasma gondii by PCR (0055591) may also be useful if a specimen can be collected from an affected body site.
Result Interpretation

Reference Interval

7.9 AU/mL or less:Not Detected.
8.0-9.9 AU/mL:Indeterminate - Repeat testing in 10-14 days may be helpful.
10.0 AU/mL or greater:Detected - Significant level of Toxoplasma gondii IgM antibody detected and may indicate a current or recent infection. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Interpretive Data

This test is performed using the DiaSorin LIAISON. As suggested by the CDC, any indeterminate or detected Toxoplasma gondii IgM result should be retested in parallel with a specimen collected 1-3 weeks later. Further confirmation may be necessary using a different test from another reference laboratory specializing in toxoplasmosis testing where an IgM ELISA should be ordered. Caution should be exercised in the use of IgM antibody levels in prenatal screening. Any Toxoplasma gondii IgM in pregnant patients that have also been confirmed by a second reference laboratory should be evaluated by amniocentesis and PCR testing for Toxoplasma gondii.

For male and non-pregnant female patients with indeterminate or detected Toxoplasma gondii IgM results, PCR may also be useful if a specimen can be collected from an affected body site.

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

For additional information, refer to the CDC website: www.cdc.gov/parasites/toxoplasmosis/health_professionals/index.html.

The magnitude of the measured result is not indicative of the amount of antibody present.

Administrative

CPT Codes

86778

TOXOPLASMA GONDII BY PCR

LAB3188

Collect

Lavender (EDTA), pink (K2EDTA) or serum separator tube. OR Amniotic fluid, CSF, ocular fluid or tissue.

Specimen Preparation

Separate serum or plasma from cells. Transfer 1 mL serum, plasma, amniotic fluid, CSF or ocular fluid to a sterile container. (Min: 0.5 mL) OR Tissue: Transfer to a sterile container and freeze immediately.

Unacceptable Conditions

Heparinized specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 3 months
All Others: Ambient: 8 hours; Refrigerated: 5 days;  Frozen: 3 months

Remarks

Specimen source required.

Performed

Tue, Fri

Methodology

Qualitative Polymerase Chain Reaction

Reported

1-5 days

Synonyms

  • LAB3188
  • TOXO PCR

Ordering Recommendations

Confirm toxoplasmosis infection in immunocompromised hosts as well as fetuses and newborns. May be used to confirm equivocal antibody testing.

CPT Codes

87798
Collection

LAB3188

Collect

Lavender (EDTA), pink (K2EDTA) or serum separator tube. OR Amniotic fluid, CSF, ocular fluid or tissue.

Specimen Preparation

Separate serum or plasma from cells. Transfer 1 mL serum, plasma, amniotic fluid, CSF or ocular fluid to a sterile container. (Min: 0.5 mL) OR Tissue: Transfer to a sterile container and freeze immediately.

Unacceptable Conditions

Heparinized specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 3 months
All Others: Ambient: 8 hours; Refrigerated: 5 days;  Frozen: 3 months

Remarks

Specimen source required.
Ordering

Performed

Tue, Fri

Methodology

Qualitative Polymerase Chain Reaction

Reported

1-5 days

Synonyms

  • LAB3188
  • TOXO PCR

Ordering Recommendations

Confirm toxoplasmosis infection in immunocompromised hosts as well as fetuses and newborns. May be used to confirm equivocal antibody testing.
Result Interpretation
Administrative

CPT Codes

87798

TOXOPLASMA IGG ANTIBODIES

LAB501

TOXO IGG

Collect

One 4.5 mL Red or Gold top tube with gel separator (SST).

Pediatric Collection

3 mL minimum volume.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Allow to clot for 30 minutes before centrifuging. Centrifuge within 2 hours of collection. Separate serum or plasma from cells as soon as possible. Send at 2-8 degrees C.

Stability (from collection to initiation)

Ambient: 7 days; Refrigerated:14 days.

Performed by

PCL Clinical Laboratory - Core

Performed

Upon receipt

Methodology

Chemilumenescent immunoassay

Reported

Same day

Synonyms

  • LAB501
  • TOXO IGG

Reference Interval

Negative

Interpretive Data

An equivocal result may be due to very low levels of circulating IgG during the acute stage of infection. Obtain a new specimen for repeat testing within 3 weeks, if clinically indicated.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

86777

Collection

LAB501

TOXO IGG

Collect

One 4.5 mL Red or Gold top tube with gel separator (SST).

Pediatric Collection

3 mL minimum volume.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Allow to clot for 30 minutes before centrifuging. Centrifuge within 2 hours of collection. Separate serum or plasma from cells as soon as possible. Send at 2-8 degrees C.

Stability (from collection to initiation)

Ambient: 7 days; Refrigerated:14 days.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Upon receipt

Methodology

Chemilumenescent immunoassay

Reported

Same day

Synonyms

  • LAB501
  • TOXO IGG
Result Interpretation

Reference Interval

Negative

Interpretive Data

An equivocal result may be due to very low levels of circulating IgG during the acute stage of infection. Obtain a new specimen for repeat testing within 3 weeks, if clinically indicated.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

86777

TRANS RXN IMMEDIATE INVESTIG

Collect

One 6 mL Pink top ltube (EDTA) labeled with last name, first name, MR#, date drawn, and phlebotomist's initials.

Also acceptable: One 4 mL Purple top tube (EDTA) labeled with last name, first name, MR#, date drawn, and phlebotomist's initials..

Pediatric Collection

3 mL minimum volume.

Unacceptable Conditions

Specimen not properly identified/inadequately labeled; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.

Offsite: Transport immediately at ambient temperature.

Stability (from collection to initiation)

Ambient: 1 hour.

Remarks

If patient develops chills, fever, hypotension, dyspnea, nausea, etc, stop the transfusion, keep I.V. open with saline and notify physician and Blood Bank immediately. Return blood bag and attatched administration set to Blood Bank.

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

Performed

Daily

Methodology

Hemagglutination.

Reported

Daily

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)

CPT Codes

86880
Collection

Collect

One 6 mL Pink top ltube (EDTA) labeled with last name, first name, MR#, date drawn, and phlebotomist's initials.

Also acceptable: One 4 mL Purple top tube (EDTA) labeled with last name, first name, MR#, date drawn, and phlebotomist's initials..

Pediatric Collection

3 mL minimum volume.

Unacceptable Conditions

Specimen not properly identified/inadequately labeled; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.

Offsite: Transport immediately at ambient temperature.

Stability (from collection to initiation)

Ambient: 1 hour.

Remarks

If patient develops chills, fever, hypotension, dyspnea, nausea, etc, stop the transfusion, keep I.V. open with saline and notify physician and Blood Bank immediately. Return blood bag and attatched administration set to Blood Bank.

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)
Ordering

Performed

Daily

Methodology

Hemagglutination.

Reported

Daily
Result Interpretation

Performed by

PCL Clinical Laboratory - Transfusion Services (Blood Bank)
Administrative

CPT Codes

86880

TRANSFERRIN

LAB133

TRANSFER

Collect

One 4.5 mL Green top tube with gel separator (PST).

Pediatric Collection

1.5 mL blood.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection. Send at 2-8 degrees C.

Stability (from collection to initiation)

Refrigerated: 8 days; Frozen (-20 degrees C): 1 year, if frozen within 24 hours of collection.

Performed by

PCL Clinical Laboratory - Core

Performed

Upon receipt

Methodology

Immunoturbidimetric

Reported

Daily

Synonyms

  • LAB133

Reference Interval

Effective 05/20/2014:

203 - 362 mg/dL

 

Before 05/20/2014:

Gender Low High Units
F 192 382 mg/dL
M 180 329 mg/dL

Interpretive Data

Adult reference range.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84466

Collection

LAB133

TRANSFER

Collect

One 4.5 mL Green top tube with gel separator (PST).

Pediatric Collection

1.5 mL blood.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection. Send at 2-8 degrees C.

Stability (from collection to initiation)

Refrigerated: 8 days; Frozen (-20 degrees C): 1 year, if frozen within 24 hours of collection.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Upon receipt

Methodology

Immunoturbidimetric

Reported

Daily

Synonyms

  • LAB133
Result Interpretation

Reference Interval

Effective 05/20/2014:

203 - 362 mg/dL

 

Before 05/20/2014:

Gender Low High Units
F 192 382 mg/dL
M 180 329 mg/dL

Interpretive Data

Adult reference range.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84466

TRANSPLANT ABUSE URINE TOXICOLOGY SCREEN (UTOX PANEL FOR TX)

LAB3291

DOA TRANS

Collect

20 mL random urine in a plastic urine container.

Larger volumes are encouraged to provide enough sample for confirmation testing if needed.

Pediatric Collection

Minimum volume: 15 mL urine

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; paper cups; adulterated specimens.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport at 2-8 C.

Stability (from collection to initiation)

Ambient: 2 days, Refrigerated: 7 days, Frozen: Indefinitely.

Remarks

Components with cut-offs include:
Amphetamine/Meth 1000 ng/mL
Barbiturate 200 ng/mL
Benzodiazipine 200 ng/mL
Cocaine 300 ng/mL
Opiates 300 ng/mL
Ethanol 10 mg/dL

Performed by

PCL Clinical Laboratory - Toxicology

Performed

Daily

Methodology

Homogenous enzyme immunoassay.

Reported

Same day

Synonyms

  • TRANSPLANT TOXICOLOGY SCREEN
  • LAB3291
  • DOA TRANS
  • TRANSPLANT DRUG SCREEN

Interpretive Data

Effective 05/20/2014:

The above results were obtained by immunoassay.  Definitive confirmatory testing will be performed and reported separately only for positive Barbiturates, cocaine, opiates, or ethanol results.  Unexpected negative screening results occur occasionally for Benzodiazepines.

SUBSTANCE (POSITIVE CUT-OFF VALUE)
Amphetamine/Methamph (1000 ng/mL)
Barbiturate (200 ng/mL)
Benzodiazepine (200 ng/mL)
Cocaine (300 ng/mL)
Opiates (300 ng/mL)
Ethanol (10 mg/dL)

Performed by

PCL Clinical Laboratory - Toxicology

CPT Codes

80101 (6)

Medicare patients: G0434 (1)

Collection

LAB3291

DOA TRANS

Collect

20 mL random urine in a plastic urine container.

Larger volumes are encouraged to provide enough sample for confirmation testing if needed.

Pediatric Collection

Minimum volume: 15 mL urine

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; paper cups; adulterated specimens.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport at 2-8 C.

Stability (from collection to initiation)

Ambient: 2 days, Refrigerated: 7 days, Frozen: Indefinitely.

Remarks

Components with cut-offs include:
Amphetamine/Meth 1000 ng/mL
Barbiturate 200 ng/mL
Benzodiazipine 200 ng/mL
Cocaine 300 ng/mL
Opiates 300 ng/mL
Ethanol 10 mg/dL

Performed by

PCL Clinical Laboratory - Toxicology

Ordering

Performed

Daily

Methodology

Homogenous enzyme immunoassay.

Reported

Same day

Synonyms

  • TRANSPLANT TOXICOLOGY SCREEN
  • LAB3291
  • DOA TRANS
  • TRANSPLANT DRUG SCREEN
Result Interpretation

Interpretive Data

Effective 05/20/2014:

The above results were obtained by immunoassay.  Definitive confirmatory testing will be performed and reported separately only for positive Barbiturates, cocaine, opiates, or ethanol results.  Unexpected negative screening results occur occasionally for Benzodiazepines.

SUBSTANCE (POSITIVE CUT-OFF VALUE)
Amphetamine/Methamph (1000 ng/mL)
Barbiturate (200 ng/mL)
Benzodiazepine (200 ng/mL)
Cocaine (300 ng/mL)
Opiates (300 ng/mL)
Ethanol (10 mg/dL)

Performed by

PCL Clinical Laboratory - Toxicology

Administrative

CPT Codes

80101 (6)

Medicare patients: G0434 (1)

TRANSPLANT CD3

LAB3054

Collect

One 4-7 mL dark green top (sodium heparin plasma) AND one 4 mL purple top (EDTA plasma).

Specimen Preparation

Please Note: On patients with no identified lymph population by CBC analysis (absolute lymphocyte count of 0) and a white count under 5,000 cells e9/L, the TXCD3 will be cancelled due to lack of T cells to phenotype.

Pediatric Collection

3 mL green top (sodium heparin) AND 0.5 mL blood in a microtainer, pink top, plasma (EDTA).

Unacceptable Conditions

Specimens older than 24 hours; clotted, frozen, or hemolyzed specimens; specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport at ambient temperature.

Stability (from collection to initiation)

Ambient: Green top tube for CD3 analysis 24 hours; Purple top tube for absolute lymphocyte count 8 hours.

 

Refrigerated: Purple top tube for absolute lymphocyte count 36 hours.

Remarks

Stat requests have a turn-around time of 24 hours.

 

TXCD3 requires an Absolute Lymphocyte Count. A Complete Blood Count (CBC) and a differential (DIFF) must be ordered and collected at the same time.

Performed

Daily; Sat/Sun must be received before 1pm

Methodology

Flow Cytometry

Reported

Same day

Synonyms

  • CD3 - TRANSPLANT CD3
  • LAB3054

CPT Codes

86356, 86359

Collection

LAB3054

Collect

One 4-7 mL dark green top (sodium heparin plasma) AND one 4 mL purple top (EDTA plasma).

Specimen Preparation

Please Note: On patients with no identified lymph population by CBC analysis (absolute lymphocyte count of 0) and a white count under 5,000 cells e9/L, the TXCD3 will be cancelled due to lack of T cells to phenotype.

Pediatric Collection

3 mL green top (sodium heparin) AND 0.5 mL blood in a microtainer, pink top, plasma (EDTA).

Unacceptable Conditions

Specimens older than 24 hours; clotted, frozen, or hemolyzed specimens; specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport at ambient temperature.

Stability (from collection to initiation)

Ambient: Green top tube for CD3 analysis 24 hours; Purple top tube for absolute lymphocyte count 8 hours.

 

Refrigerated: Purple top tube for absolute lymphocyte count 36 hours.

Remarks

Stat requests have a turn-around time of 24 hours.

 

TXCD3 requires an Absolute Lymphocyte Count. A Complete Blood Count (CBC) and a differential (DIFF) must be ordered and collected at the same time.

Ordering

Performed

Daily; Sat/Sun must be received before 1pm

Methodology

Flow Cytometry

Reported

Same day

Synonyms

  • CD3 - TRANSPLANT CD3
  • LAB3054
Result Interpretation
Administrative

CPT Codes

86356, 86359

TREPONEMA ANTIBODIES

LAB1197
TREP AB

Collect

One 7.0 mL gold top tube with gel (SST), clot activator serum

Pediatric Collection

Minimum volume 1.0 mL serum

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; pooled samples; grossly  hemolyzed samples; obvious microbial contamination; heat-inactivated samples; cadaver specimens; other body fluids.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.

Offsite: Allow to clot for 30 minutes before centrifuging. Centrifuge within 2 hours of collection. Transport to lab at 2-8 degrees C.  If transport time exceeds 2 days, place serum in aliquot tube and transport frozen.

Stability (from collection to initiation)

Before centrifugation: Ambient, 2 hours.
After centrifugation: Ambient, 3 days; Referigerated, 7 days.
Serum aliquot tube: Ambient, 3 days; Refrigerated, 7 days; Frozen (-70 degrees C), 6 months.

Remarks

Samples that test reactive by the TREPAB assay will automatically be tested by the rapid plasma reagin (RPR) method to help determine the timing of the infection. If the RPR test is reactive, RPR titer will be performed.

Performed by

PCL Clinical Laboratory - Toxicology

Performed

Daily, Sunday through Saturday

Methodology

Chemiluminescent microparticle immunoassay (CMIA)

Reported

Same day

Synonyms

  • RPR Titer
  • Trep IgG/IgM AB
  • RPR
  • Syphilis
  • TREPAB
  • TREP AB
  • Treponema pallidum

Reference Interval

Non Reactive

Performed by

PCL Clinical Laboratory - Toxicology

CPT Codes

TREPAB 86780.  If reactive, RPR will be billed (86592). If RPR reactive, RPR Titer will be billed (86953).

Collection

LAB1197
TREP AB

Collect

One 7.0 mL gold top tube with gel (SST), clot activator serum

Pediatric Collection

Minimum volume 1.0 mL serum

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; pooled samples; grossly  hemolyzed samples; obvious microbial contamination; heat-inactivated samples; cadaver specimens; other body fluids.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.

Offsite: Allow to clot for 30 minutes before centrifuging. Centrifuge within 2 hours of collection. Transport to lab at 2-8 degrees C.  If transport time exceeds 2 days, place serum in aliquot tube and transport frozen.

Stability (from collection to initiation)

Before centrifugation: Ambient, 2 hours.
After centrifugation: Ambient, 3 days; Referigerated, 7 days.
Serum aliquot tube: Ambient, 3 days; Refrigerated, 7 days; Frozen (-70 degrees C), 6 months.

Remarks

Samples that test reactive by the TREPAB assay will automatically be tested by the rapid plasma reagin (RPR) method to help determine the timing of the infection. If the RPR test is reactive, RPR titer will be performed.

Performed by

PCL Clinical Laboratory - Toxicology

Ordering

Performed

Daily, Sunday through Saturday

Methodology

Chemiluminescent microparticle immunoassay (CMIA)

Reported

Same day

Synonyms

  • RPR Titer
  • Trep IgG/IgM AB
  • RPR
  • Syphilis
  • TREPAB
  • TREP AB
  • Treponema pallidum
Result Interpretation

Reference Interval

Non Reactive

Performed by

PCL Clinical Laboratory - Toxicology

Administrative

CPT Codes

TREPAB 86780.  If reactive, RPR will be billed (86592). If RPR reactive, RPR Titer will be billed (86953).

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