Tests Catalogs

PAIN MANAGEMENT URINE TOX SCREEN

LAB3726

This test is only for use by the Pain Managment clinics.

Effective 3/2/15: This testing will be done by PCL Clinical Laboratory.

Collect

Random urine.

Specimen Preparation

Transfer 4 mL each into two (2) ARUP Standard Transport Tubes urine with no additives or preservatives. (Min: 2 mL each)

Unacceptable Conditions

Specimens exposed to repeated freeze/thaw cycles.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years

Performed by

PCL Clinical Laboratory in Salt Lake City,UT.

Notes

Creatinine concentration is also provided. The carisoprodol immunoassay has cross-reactivity to carisoprodol and meprobamate.

Performed

Sun-Sat

Methodology

Qualitative Liquid Chromatography/Time of Flight Mass Spectrometry/Enzyme Immunoassay/Quantitative Spectrophotometry

Reported

1-3 days

Synonyms

  • LAB3726
  • PAIN HYB U
  • URPAINTOX

Reference Interval

Effective November 17, 2014
 

Drugs covered and range of cutoff concentrations. Note that some drugs are identified based on the presence of unique drug metabolites not listed below.
 
Drugs/Drug Classes Range of Cutoff Concentrations
Barbiturates 200 ng/mL
Benzodiazepine-​like:
alprazolam, clonazepam, diazepam, lorazepam, midazolam, nordiazepam, oxazepam, temazepam, zolpidem 		20 -​ 60 ng/mL
Cannabinoids (11-​nor-​9-​carboxy-​THC) 20 ng/mL
Ethyl Glucuronide 500 ng/mL
Muscle Relaxant(s):
carisoprodol, meprobamate 		100 ng/mL
Opiates/Opioids:
buprenorphine, codeine, fentanyl, heroin, hydrocodone, hydromorphone, meperidine, methadone, morphine, oxycodone, oxymorphone, propoxyphene, tapentadol, tramadol 		2-​300 ng/mL
Phencyclidine (PCP) 25 ng/mL
Stimulants:
amphetamine, cocaine, methamphetamine, methylphenidate, MDMA (Ecstasy), MDEA (Eve), MDA, phentermine 		100-​400 ng/mL

Interpretive Data

Methodology: Qualitative Enzyme Immunoassay and Qualitative Liquid Chromatography-Time-of-Flight-Mass Spectrometry, Quantitative Spectrophotometry

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration must be greater than or equal to the cutoff concentration to be reported as present. If specific drug concentrations are required, contact the laboratory within two weeks of specimen collection to request confirmation and quantification by a second analytical technique. Interpretive questions should be directed to the laboratory.

Results based on immunoassay detection that do not match clinical expectations should be interpreted with caution. Confirmatory testing by mass spectrometry for immunoassay-based results is available, if ordered within two weeks of specimen collection. Additional charges apply.

For medical purposes only; not valid for forensic use.

Performed by

PCL Clinical Laboratory in Salt Lake City,UT.

CPT Codes

80304; 80301; 80302 x3; (Alt code: G0431)
Collection

LAB3726

This test is only for use by the Pain Managment clinics.

Effective 3/2/15: This testing will be done by PCL Clinical Laboratory.

Collect

Random urine.

Specimen Preparation

Transfer 4 mL each into two (2) ARUP Standard Transport Tubes urine with no additives or preservatives. (Min: 2 mL each)

Unacceptable Conditions

Specimens exposed to repeated freeze/thaw cycles.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years

Performed by

PCL Clinical Laboratory in Salt Lake City,UT.

Notes

Creatinine concentration is also provided. The carisoprodol immunoassay has cross-reactivity to carisoprodol and meprobamate.
Ordering

Performed

Sun-Sat

Methodology

Qualitative Liquid Chromatography/Time of Flight Mass Spectrometry/Enzyme Immunoassay/Quantitative Spectrophotometry

Reported

1-3 days

Synonyms

  • LAB3726
  • PAIN HYB U
  • URPAINTOX
Result Interpretation

Reference Interval

Effective November 17, 2014
 

Drugs covered and range of cutoff concentrations. Note that some drugs are identified based on the presence of unique drug metabolites not listed below.
 
Drugs/Drug Classes Range of Cutoff Concentrations
Barbiturates 200 ng/mL
Benzodiazepine-​like:
alprazolam, clonazepam, diazepam, lorazepam, midazolam, nordiazepam, oxazepam, temazepam, zolpidem 		20 -​ 60 ng/mL
Cannabinoids (11-​nor-​9-​carboxy-​THC) 20 ng/mL
Ethyl Glucuronide 500 ng/mL
Muscle Relaxant(s):
carisoprodol, meprobamate 		100 ng/mL
Opiates/Opioids:
buprenorphine, codeine, fentanyl, heroin, hydrocodone, hydromorphone, meperidine, methadone, morphine, oxycodone, oxymorphone, propoxyphene, tapentadol, tramadol 		2-​300 ng/mL
Phencyclidine (PCP) 25 ng/mL
Stimulants:
amphetamine, cocaine, methamphetamine, methylphenidate, MDMA (Ecstasy), MDEA (Eve), MDA, phentermine 		100-​400 ng/mL

Interpretive Data

Methodology: Qualitative Enzyme Immunoassay and Qualitative Liquid Chromatography-Time-of-Flight-Mass Spectrometry, Quantitative Spectrophotometry

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration must be greater than or equal to the cutoff concentration to be reported as present. If specific drug concentrations are required, contact the laboratory within two weeks of specimen collection to request confirmation and quantification by a second analytical technique. Interpretive questions should be directed to the laboratory.

Results based on immunoassay detection that do not match clinical expectations should be interpreted with caution. Confirmatory testing by mass spectrometry for immunoassay-based results is available, if ordered within two weeks of specimen collection. Additional charges apply.

For medical purposes only; not valid for forensic use.

Performed by

PCL Clinical Laboratory in Salt Lake City,UT.

Administrative

CPT Codes

80304; 80301; 80302 x3; (Alt code: G0431)

PANCREATIC POLYPEPTIDE

LAB3108

 

Collect

Serum separator tube or plain red.

Patient Preparation

Patient should be fasting for 10 hours prior to collection of specimen.

Specimen Preparation

Allow specimen to sit in collection tube for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Plasma. Severely hemolyzed or lipemic specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: 24 hours; Refrigerated: 24 hours; Frozen: 2 months

Performed

Wed

Methodology

Quantitative Radioimmunoassay

Reported

3-11 days

Synonyms

  • LAB3108
  • PAN POLY
  • PANPP
  • PANCREATIC

Ordering Recommendations

Aids in the diagnosis and monitoring of pancreatic neuroendocrine tumors.

Reference Interval

0-435 pg/mL

CPT Codes

83519
Collection

LAB3108

 

Collect

Serum separator tube or plain red.

Patient Preparation

Patient should be fasting for 10 hours prior to collection of specimen.

Specimen Preparation

Allow specimen to sit in collection tube for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Plasma. Severely hemolyzed or lipemic specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: 24 hours; Refrigerated: 24 hours; Frozen: 2 months
Ordering

Performed

Wed

Methodology

Quantitative Radioimmunoassay

Reported

3-11 days

Synonyms

  • LAB3108
  • PAN POLY
  • PANPP
  • PANCREATIC

Ordering Recommendations

Aids in the diagnosis and monitoring of pancreatic neuroendocrine tumors.
Result Interpretation

Reference Interval

0-435 pg/mL
Administrative

CPT Codes

83519

PARALDEHYDE + ACETALDEHYDE

LAB3224

 

Collect

Gray (potassium oxalate/sodium fluoride) or plain red.

Specimen Preparation

Separate plasma or serum from cells ASAP. Transfer 2 mL plasma or serum to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.7 mL).

Unacceptable Conditions

Thawed specimens. Separator tubes.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Undetermined

Performed

Varies

Methodology

Quantitative Gas Chromatography

Reported

3-6 days

Synonyms

  • Paral{R}
  • LAB3224
  • PARALDEHYDE
  • PARALD/ACE

Reference Interval

By report

CPT Codes

80375 (Alt code: G0480)
Collection

LAB3224

 

Collect

Gray (potassium oxalate/sodium fluoride) or plain red.

Specimen Preparation

Separate plasma or serum from cells ASAP. Transfer 2 mL plasma or serum to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.7 mL).

Unacceptable Conditions

Thawed specimens. Separator tubes.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Undetermined
Ordering

Performed

Varies

Methodology

Quantitative Gas Chromatography

Reported

3-6 days

Synonyms

  • Paral{R}
  • LAB3224
  • PARALDEHYDE
  • PARALD/ACE
Result Interpretation

Reference Interval

By report
Administrative

CPT Codes

80375 (Alt code: G0480)

PARANEOPLASTIC AB PANEL-CSF

LAB3336

 

Collect

3.0 mL CSF

Minimum volume = 2.0 mL

Unacceptable Conditions

.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 3 mL CSF Deliver to lab at ambient temperature.

Stability (from collection to initiation)

Stability:  Ambient < 3 days; Refrigerated < 14 days: Frozen OK

Remarks

Paraneoplastic antibodies tested:

Anti-Neuronal Nuclear Ab Type 1
Anti-Neuronal Nuclear Ab Type 2
Anti-Neuronal Nuclear Ab Type 3
Anti-Glial Nuclear Ab Type 1
Purkinje Cell Cytoplasmic Ab Type 1
Purkinje Cell Cytoplasmic Ab Type 2

Purkinje Cell Cytoplasmic Ab Type Tr

Amphiphysin Ab

Test Algorithm:

If indirect immunofluorescence assay (IFA) (ANN1C, ANN2C, ANN3C, PCA1C, PCA2C, PCTRC, AMPHC, CRMC, AGN1C) is indeterminate, paraneoplastic autoantibody Western blot is performed at an additional charge.

If IFA pattern suggest NMO/AQP4-IgG, NMO/AQP4-IgG CBA is performed at an additional charge.

If client requests or if IFA patterns suggest CRMP-5-IgG, CRMP-5-IgG Western blot is performed at an additional charge.

If IFA patterns suggest GAD65 antibody, GAD65 antibody radioimmunoprecipitation assay is performed at an additional charge.

If IFA patterns suggest amphiphysin antibody, amphiphysin Western blot is performed at an additional charge.

 

Performed by

Mayo Medical Laboratories

Performed

Mon - Fri

Methodology

Indirect Immunofluorescence Assay (IFA)

Reported

In 6 days

Synonyms

  • LAB3336
  • PARANEOCSF

Performed by

Mayo Medical Laboratories

CPT Codes

86256 x8

Collection

LAB3336

 

Collect

3.0 mL CSF

Minimum volume = 2.0 mL

Unacceptable Conditions

.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 3 mL CSF Deliver to lab at ambient temperature.

Stability (from collection to initiation)

Stability:  Ambient < 3 days; Refrigerated < 14 days: Frozen OK

Remarks

Paraneoplastic antibodies tested:

Anti-Neuronal Nuclear Ab Type 1
Anti-Neuronal Nuclear Ab Type 2
Anti-Neuronal Nuclear Ab Type 3
Anti-Glial Nuclear Ab Type 1
Purkinje Cell Cytoplasmic Ab Type 1
Purkinje Cell Cytoplasmic Ab Type 2

Purkinje Cell Cytoplasmic Ab Type Tr

Amphiphysin Ab

Test Algorithm:

If indirect immunofluorescence assay (IFA) (ANN1C, ANN2C, ANN3C, PCA1C, PCA2C, PCTRC, AMPHC, CRMC, AGN1C) is indeterminate, paraneoplastic autoantibody Western blot is performed at an additional charge.

If IFA pattern suggest NMO/AQP4-IgG, NMO/AQP4-IgG CBA is performed at an additional charge.

If client requests or if IFA patterns suggest CRMP-5-IgG, CRMP-5-IgG Western blot is performed at an additional charge.

If IFA patterns suggest GAD65 antibody, GAD65 antibody radioimmunoprecipitation assay is performed at an additional charge.

If IFA patterns suggest amphiphysin antibody, amphiphysin Western blot is performed at an additional charge.

 

Performed by

Mayo Medical Laboratories

Ordering

Performed

Mon - Fri

Methodology

Indirect Immunofluorescence Assay (IFA)

Reported

In 6 days

Synonyms

  • LAB3336
  • PARANEOCSF
Result Interpretation

Performed by

Mayo Medical Laboratories

Administrative

CPT Codes

86256 x8

PARANEOPLASTIC AB PANEL-SERUM

LAB3099

Collect

10 mL gold top tube with gel (serum separator tube)or plain red top tube.

Unacceptable Conditions

Plasma.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 3 mL serum Spin, separate and deliver to lab on wet ice or at ambient temperature if to be received by lab within 24 hours.

Stability (from collection to initiation)

Stability:  Ambient < 3 days; Refrigerated < 14 days: Frozen OK

Remarks

Paraneoplastic antibodies tested:
Anti-Neuronal Nuclear Ab, Types 1, 2, 3
Anti-Glial Nuclear Ab, Type 1 (AGNA-1)
Purkinje Cell Cytoplasmic Ab, Types 1, 2, Tr
Amphyphysin Ab

Voltage-Gated Calcium Channel Ab, Types N, P/Q

Voltage-Gated Potassium Channel Ab

CRMP-5-IgG Ab

ACHR Ganglionic

ACHR Binding Ab

Striated Muscle Ab

Reflex Testing Algorithm:
If IFA (ANN1S, ANN2S, ANN3S, PCABP, PCAB2, PCATR, AMPHS, CRMS, AGN1S) patterns are indeterminate, paraneoplastic autoantibody Western blot is performed at an additional charge.

If IFA patterns suggest CRMP-5-IgG, CRMP-5-IgG Western blot is performed at an additional charge.

If IFA pattern suggest NMO/AQP4-IgG, NMO/AQP4-IgG FACS is performed at an additional charge.

If NMO/AQP4-IgG FACS screen assay is positive, then NMO/AQP4-IgG FACS titration assay is performed at an additional charge.

If IFA patterns suggest amphiphysin antibody, amphiphysin Western blot is performed at an additional charge.

If IFA patterns suggest GAD65 antibody, GAD65 antibody radioimmunoassay is performed at an additional charge.

If IFA pattern suggest NMDA-R, NMDA-R Ab CBA and/or NMDA-R Ab IF Titer Assay is performed at an additional charge.

If IFA pattern suggest AMPA-R, AMPA-R Ab CBA and/or AMPA-R Ab IF Titer Assay is performed at an additional charge.

If IFA pattern suggest GABA-B-R, GABA-B-R Ab CBA and/or GABA-B-R Ab IF Titer Assay is performed at an additional charge.

If ACh receptor binding antibody is >0.02, ACh receptor modulating antibodies and CRMP-5-IgG Western blot are performed at an additional charge.

CRMP-5-IgG Western blot is also performed by specific request for more sensitive detection of CRMP-5-IgG. Testing should be requested in cases of subacute basal ganglionic disorders (chorea, Parkinsonism), cranial neuropathies (especially loss of vision, taste, or smell) and myelopathies.

Performed by

Mayo Medical Laboratories

Performed

Mon - Fri

Methodology

Indirect Immunofluorescence Assay (IFA)

Reported

10 - 17 days

Synonyms

  • AMPHIPHYSIN ANTIBODY
  • LAB3099
  • PARANEOSER

Performed by

Mayo Medical Laboratories

CPT Codes

83520, 83519 x5, 86256 x9

Collection

LAB3099

Collect

10 mL gold top tube with gel (serum separator tube)or plain red top tube.

Unacceptable Conditions

Plasma.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 3 mL serum Spin, separate and deliver to lab on wet ice or at ambient temperature if to be received by lab within 24 hours.

Stability (from collection to initiation)

Stability:  Ambient < 3 days; Refrigerated < 14 days: Frozen OK

Remarks

Paraneoplastic antibodies tested:
Anti-Neuronal Nuclear Ab, Types 1, 2, 3
Anti-Glial Nuclear Ab, Type 1 (AGNA-1)
Purkinje Cell Cytoplasmic Ab, Types 1, 2, Tr
Amphyphysin Ab

Voltage-Gated Calcium Channel Ab, Types N, P/Q

Voltage-Gated Potassium Channel Ab

CRMP-5-IgG Ab

ACHR Ganglionic

ACHR Binding Ab

Striated Muscle Ab

Reflex Testing Algorithm:
If IFA (ANN1S, ANN2S, ANN3S, PCABP, PCAB2, PCATR, AMPHS, CRMS, AGN1S) patterns are indeterminate, paraneoplastic autoantibody Western blot is performed at an additional charge.

If IFA patterns suggest CRMP-5-IgG, CRMP-5-IgG Western blot is performed at an additional charge.

If IFA pattern suggest NMO/AQP4-IgG, NMO/AQP4-IgG FACS is performed at an additional charge.

If NMO/AQP4-IgG FACS screen assay is positive, then NMO/AQP4-IgG FACS titration assay is performed at an additional charge.

If IFA patterns suggest amphiphysin antibody, amphiphysin Western blot is performed at an additional charge.

If IFA patterns suggest GAD65 antibody, GAD65 antibody radioimmunoassay is performed at an additional charge.

If IFA pattern suggest NMDA-R, NMDA-R Ab CBA and/or NMDA-R Ab IF Titer Assay is performed at an additional charge.

If IFA pattern suggest AMPA-R, AMPA-R Ab CBA and/or AMPA-R Ab IF Titer Assay is performed at an additional charge.

If IFA pattern suggest GABA-B-R, GABA-B-R Ab CBA and/or GABA-B-R Ab IF Titer Assay is performed at an additional charge.

If ACh receptor binding antibody is >0.02, ACh receptor modulating antibodies and CRMP-5-IgG Western blot are performed at an additional charge.

CRMP-5-IgG Western blot is also performed by specific request for more sensitive detection of CRMP-5-IgG. Testing should be requested in cases of subacute basal ganglionic disorders (chorea, Parkinsonism), cranial neuropathies (especially loss of vision, taste, or smell) and myelopathies.

Performed by

Mayo Medical Laboratories

Ordering

Performed

Mon - Fri

Methodology

Indirect Immunofluorescence Assay (IFA)

Reported

10 - 17 days

Synonyms

  • AMPHIPHYSIN ANTIBODY
  • LAB3099
  • PARANEOSER
Result Interpretation

Performed by

Mayo Medical Laboratories

Administrative

CPT Codes

83520, 83519 x5, 86256 x9

PARASITE EXAM - MACROSCOPIC

LAB3273

 

Collect

Worm or proglottid.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: ASAP

Remarks

Any stool received with worm should be preserved in formalin.

Performed by

PCL Clinical Laboratory

Performed

Daily, dayshift

Methodology

Direct examination.

Reported

Within 24 hours

Synonyms

  • LAB3273

Performed by

PCL Clinical Laboratory

CPT Codes

87169

Collection

LAB3273

 

Collect

Worm or proglottid.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: ASAP

Remarks

Any stool received with worm should be preserved in formalin.

Performed by

PCL Clinical Laboratory

Ordering

Performed

Daily, dayshift

Methodology

Direct examination.

Reported

Within 24 hours

Synonyms

  • LAB3273
Result Interpretation

Performed by

PCL Clinical Laboratory

Administrative

CPT Codes

87169

PARATHYROID HORMONE-INTACT WITHOUT CALCIUM

LAB108

PTH INT

 

Collect

One 4.5 mL Green top tube with gel separator (PST).

Pediatric Collection

0.2 mL plasma.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection. Transport refrigerated.

Stability (from collection to initiation)

Whole blood - Ambient: 2 hours.

Plasma - Ambient: 8 hours; Refrigerated: 48 hours.

Performed by

PCL Clinical Laboratory - Core

Notes

Request this test for Parathyroid Hormone value only. Total Calcium is not performed or reported.

Performed

Upon receipt

Methodology

Chemiluminescent Immunoassay

Reported

Same day

Synonyms

  • LAB108

Reference Interval

Gender Age Range Normal Range Units
M/F 19 - 67 years 12 -88 pg/mL

Reference ranges have not been established for subjects under 19 years and over 67 years of age.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

83970

Collection

LAB108

PTH INT

 

Collect

One 4.5 mL Green top tube with gel separator (PST).

Pediatric Collection

0.2 mL plasma.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection. Transport refrigerated.

Stability (from collection to initiation)

Whole blood - Ambient: 2 hours.

Plasma - Ambient: 8 hours; Refrigerated: 48 hours.

Performed by

PCL Clinical Laboratory - Core

Notes

Request this test for Parathyroid Hormone value only. Total Calcium is not performed or reported.

Ordering

Performed

Upon receipt

Methodology

Chemiluminescent Immunoassay

Reported

Same day

Synonyms

  • LAB108
Result Interpretation

Reference Interval

Gender Age Range Normal Range Units
M/F 19 - 67 years 12 -88 pg/mL

Reference ranges have not been established for subjects under 19 years and over 67 years of age.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

83970

PAROXYSMAL NOCTURNAL HEMOGLOBINURIA-RBC AND WBC

LAB3659

 

Collect

Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Transport 4 mL whole blood. (Min: 4 mL)

Unacceptable Conditions

Bone marrow. Clotted or hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: 48 hours. Frozen: Unacceptable

Remarks

Specimens must be analyzed within stability times provided.

Performed

Sun-Sat

Methodology

Quantitative Flow Cytometry

Reported

1-4 days

Synonyms

  • PNH PANEL - RBC and WBC
  • LAB3659
  • CD15, CD33, CD14, CD24, FLAER, CD59, Glycophorin A
  • PI-Linked Antigens, RBCs, Monocytes and Granulocytes
  • PNH Clones
  • PNH PANEL

Additional Technical Information

Ordering Recommendations

Preferred test for initial diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) and quantification of PNH clones.

Reference Interval

By report

Interpretive Data

This test is preferred for the initial diagnosis of PNH, and was developed according to published guidelines (Cytometry B Clin. Cytom. 2010; 78:211) and as updated in 2014 (Cytometry B Clin. Cytom. 2014; 86:44). The test includes high-sensitivity WBC and RBC analysis with lower limits of detection of 0.005 percent for RBCs, 0.005 percent for PMNs, and 0.020 percent for monocytes.

WBC analysis is the most accurate measurement of the PNH clone size. FLAER and CD157 are used as GPI-linked markers; CD15 (PMNs) and CD64 (monocytes) are used as lineage-specific markers. RBC analysis quantifies Type II and Type III RBC clones when the percentage of PNH RBCs is greater than 1.000. Glycophorin A (CD235a) is used to gate the RBC population, and CD59 is the GPI-linked antigen. Recent RBC transfusions may decrease the percentage of PNH cells measured in RBCs (Cytometry 2000; 42:223). The presence of a subclinical PNH population in myelodysplastic bone marrow disorders, such as aplastic anemia or refractory anemia, may correlate with a positive immunotherapeutic response (Blood 2006; 107, 1308-1314).

CPT Codes

86356 x7
Collection

LAB3659

 

Collect

Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Transport 4 mL whole blood. (Min: 4 mL)

Unacceptable Conditions

Bone marrow. Clotted or hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: 48 hours. Frozen: Unacceptable

Remarks

Specimens must be analyzed within stability times provided.
Ordering

Performed

Sun-Sat

Methodology

Quantitative Flow Cytometry

Reported

1-4 days

Synonyms

  • PNH PANEL - RBC and WBC
  • LAB3659
  • CD15, CD33, CD14, CD24, FLAER, CD59, Glycophorin A
  • PI-Linked Antigens, RBCs, Monocytes and Granulocytes
  • PNH Clones
  • PNH PANEL

Additional Technical Information

Ordering Recommendations

Preferred test for initial diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) and quantification of PNH clones.
Result Interpretation

Reference Interval

By report

Interpretive Data

This test is preferred for the initial diagnosis of PNH, and was developed according to published guidelines (Cytometry B Clin. Cytom. 2010; 78:211) and as updated in 2014 (Cytometry B Clin. Cytom. 2014; 86:44). The test includes high-sensitivity WBC and RBC analysis with lower limits of detection of 0.005 percent for RBCs, 0.005 percent for PMNs, and 0.020 percent for monocytes.

WBC analysis is the most accurate measurement of the PNH clone size. FLAER and CD157 are used as GPI-linked markers; CD15 (PMNs) and CD64 (monocytes) are used as lineage-specific markers. RBC analysis quantifies Type II and Type III RBC clones when the percentage of PNH RBCs is greater than 1.000. Glycophorin A (CD235a) is used to gate the RBC population, and CD59 is the GPI-linked antigen. Recent RBC transfusions may decrease the percentage of PNH cells measured in RBCs (Cytometry 2000; 42:223). The presence of a subclinical PNH population in myelodysplastic bone marrow disorders, such as aplastic anemia or refractory anemia, may correlate with a positive immunotherapeutic response (Blood 2006; 107, 1308-1314).

Administrative

CPT Codes

86356 x7

PARTIAL AFB STAIN

Collect

Appropriate specimen for smear is the same as for culture for Nocardia

Unacceptable Conditions

Dry swab

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: 0.5 mL Refrigerate and transport immediately.

Stability (from collection to initiation)

Room Temp: 1 hour; Refrigerated: 24-72 hours.

Remarks

Modified acid-fast stain for Nocardia. Order AFB STAIN if looking for Mycobacteria.

Performed by

PCL Clinical Laboratory

Performed

Daily

Methodology

Modified acid-fast stain

Reported

Within 24 hours

Performed by

PCL Clinical Laboratory

CPT Codes

87206
Collection

Collect

Appropriate specimen for smear is the same as for culture for Nocardia

Unacceptable Conditions

Dry swab

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: 0.5 mL Refrigerate and transport immediately.

Stability (from collection to initiation)

Room Temp: 1 hour; Refrigerated: 24-72 hours.

Remarks

Modified acid-fast stain for Nocardia. Order AFB STAIN if looking for Mycobacteria.

Performed by

PCL Clinical Laboratory
Ordering

Performed

Daily

Methodology

Modified acid-fast stain

Reported

Within 24 hours
Result Interpretation

Performed by

PCL Clinical Laboratory
Administrative

CPT Codes

87206

PARVOVIRUS B19 ANTIBODY, IGG

LAB3148

 

Collect

Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, hyperlipemic, or icteric serum.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-2 days

Synonyms

  • LAB3148
  • PARVO G
  • PARVB19G

Ordering Recommendations

Not recommended as a stand-alone test. Parvovirus B19 Antibodies, IgG and IgM is preferred (0065120).

Reference Interval

0.89 IV or less: Negative - No significant level of detectable Parvovirus B19 IgG antibody.
0.90-1.10 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.11 IV or greater: Positive - IgG antibody to Parvovirus B19 detected, which may indicate a current or past infection.

Interpretive Data

The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

CPT Codes

86747
Collection

LAB3148

 

Collect

Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, hyperlipemic, or icteric serum.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-2 days

Synonyms

  • LAB3148
  • PARVO G
  • PARVB19G

Ordering Recommendations

Not recommended as a stand-alone test. Parvovirus B19 Antibodies, IgG and IgM is preferred (0065120).
Result Interpretation

Reference Interval

0.89 IV or less: Negative - No significant level of detectable Parvovirus B19 IgG antibody.
0.90-1.10 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.11 IV or greater: Positive - IgG antibody to Parvovirus B19 detected, which may indicate a current or past infection.

Interpretive Data

The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

Administrative

CPT Codes

86747

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