PORPHYRINS, SERUM, TOTAL

LAB123

 

Collect

Green (heparin), lavender (EDTA), or plain red

Specimen Preparation

CRITICAL: Protect from light during collection, storage, and shipment.  Separate plasma or serum from cells within 1 hour of collection. Transfer 2 mL plasma or serum to an ARUP Amber Transport Tube. (Min: 1 mL)

Unacceptable Conditions

Body fluids other than plasma or serum. Frozen whole blood. Hemolyzed specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 4 days; Frozen: 1 month

Notes

Useful for evaluation of cutaneous photosensitivity to rule out porphyrin disorders, particularly erythropoietic protoporphyria. Urine is the best specimen for evaluation of suspected porphyria cutanea tarda (PCT), but monitoring of PCT with plasma or serum is an acceptable practice. Evaluation of neurologic and/or psychiatric symptoms associated with suspected acute porphyria (such as acute intermittent porphyria) requires Porphobilinogen (PBG), Urine (ARUP test code 0080260).

Specimens from patients with suspected erythropoietic protoporphyria should be carefully protected from exposure to light.
Protoporphyrin is extremely light sensitive, whereas uroporphyrin and coproporphyrin are much less sensitive.

Performed

Sun, Tue, Thu

Methodology

Quantitative Fluorometry

Reported

1-4 days

Synonyms

  • LAB123
  • POR S
  • PORPHSTOT

Ordering Recommendations

Monitor porphyria cutanea tarda (PCT). Confirm diagnosis of suspected variegate porphyria (VP) and erythropoietic protoporphyria (EPP).

Reference Interval

0-15 nmol/L

CPT Codes

84311
Collection

LAB123

 

Collect

Green (heparin), lavender (EDTA), or plain red

Specimen Preparation

CRITICAL: Protect from light during collection, storage, and shipment.  Separate plasma or serum from cells within 1 hour of collection. Transfer 2 mL plasma or serum to an ARUP Amber Transport Tube. (Min: 1 mL)

Unacceptable Conditions

Body fluids other than plasma or serum. Frozen whole blood. Hemolyzed specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 4 days; Frozen: 1 month

Notes

Useful for evaluation of cutaneous photosensitivity to rule out porphyrin disorders, particularly erythropoietic protoporphyria. Urine is the best specimen for evaluation of suspected porphyria cutanea tarda (PCT), but monitoring of PCT with plasma or serum is an acceptable practice. Evaluation of neurologic and/or psychiatric symptoms associated with suspected acute porphyria (such as acute intermittent porphyria) requires Porphobilinogen (PBG), Urine (ARUP test code 0080260).

Specimens from patients with suspected erythropoietic protoporphyria should be carefully protected from exposure to light.
Protoporphyrin is extremely light sensitive, whereas uroporphyrin and coproporphyrin are much less sensitive.
Ordering

Performed

Sun, Tue, Thu

Methodology

Quantitative Fluorometry

Reported

1-4 days

Synonyms

  • LAB123
  • POR S
  • PORPHSTOT

Ordering Recommendations

Monitor porphyria cutanea tarda (PCT). Confirm diagnosis of suspected variegate porphyria (VP) and erythropoietic protoporphyria (EPP).
Result Interpretation

Reference Interval

0-15 nmol/L
Administrative

CPT Codes

84311

PORPHYRINS, URINE

LAB433

 

Collect

24-hour or random urine. Refrigerate 24-hour specimens during collection.

Storage/Transport Temperature

4.5 mL aliquot, frozen. (Min: 2 mL) Submit specimen in an ARUP Amber Transport Tube.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 4 days; Frozen: 1 month

Remarks

The most important aspect of specimen preservation is adequate refrigeration during collection, storage, and transport.  Protect from light during collection, storage, and shipment.  Record total volume and collection time interval on transport tube and test request form.

Performed by

PCL Clinical Laboratory

Notes

Urine porphyrins are useful for the evaluation of cutaneous photosensitivity to exclude porphyria cutanea tarda (PCT). Evaluation of neurologic and/or psychiatric symptoms associated with acute porphyrias such as acute intermittent porphyria (AIP) requires urine porphobilinogen (PBG) testing. Refer to Porphobilinogen (PBG), Urine (0080260).

Performed

Sun, Tue-Sat

Methodology

High Performance Liquid Chromatography

Reported

1-4 days

Synonyms

  • Coproporphyrin, Urine
  • Uroporphyrin, Urine
  • LAB433
  • PORPHUFRAC

Performed by

PCL Clinical Laboratory

CPT Codes

84120

Collection

LAB433

 

Collect

24-hour or random urine. Refrigerate 24-hour specimens during collection.

Storage/Transport Temperature

4.5 mL aliquot, frozen. (Min: 2 mL) Submit specimen in an ARUP Amber Transport Tube.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 4 days; Frozen: 1 month

Remarks

The most important aspect of specimen preservation is adequate refrigeration during collection, storage, and transport.  Protect from light during collection, storage, and shipment.  Record total volume and collection time interval on transport tube and test request form.

Performed by

PCL Clinical Laboratory

Notes

Urine porphyrins are useful for the evaluation of cutaneous photosensitivity to exclude porphyria cutanea tarda (PCT). Evaluation of neurologic and/or psychiatric symptoms associated with acute porphyrias such as acute intermittent porphyria (AIP) requires urine porphobilinogen (PBG) testing. Refer to Porphobilinogen (PBG), Urine (0080260).

Ordering

Performed

Sun, Tue-Sat

Methodology

High Performance Liquid Chromatography

Reported

1-4 days

Synonyms

  • Coproporphyrin, Urine
  • Uroporphyrin, Urine
  • LAB433
  • PORPHUFRAC
Result Interpretation

Performed by

PCL Clinical Laboratory

Administrative

CPT Codes

84120

Posaconazole, Quantitative by LC-MS/MS

LAB8775

Collect

Plain red, lavender (EDTA), or green (sodium or lithium heparin).

Patient Preparation

Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration. Blood levels may be affected by other concurrent medications, patient conditions, fat intake at dosing, and other factors.

Specimen Preparation

Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube and freeze. (Min: 0.6 mL)

Unacceptable Conditions

Serum separator tubes. Hemolyzed or lipemic specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: 6 months

Performed

Tue, Thu, Sat

Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Reported

1-6 days

Synonyms

  • POSACONAZOLE
  • LAB8775

Additional Technical Information

Ordering Recommendations

Optimize drug therapy and monitor patient adherence.

Reference Interval

Effective August 15, 2011

Therapeutic Range (trough): Greater than 0.7 µg/mL

Interpretive Data

Adverse effects may include fever, nausea, vomiting, diarrhea, cardiac arrhythmias and liver toxicity.

CPT Codes

80299
Collection

LAB8775

Collect

Plain red, lavender (EDTA), or green (sodium or lithium heparin).

Patient Preparation

Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration. Blood levels may be affected by other concurrent medications, patient conditions, fat intake at dosing, and other factors.

Specimen Preparation

Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube and freeze. (Min: 0.6 mL)

Unacceptable Conditions

Serum separator tubes. Hemolyzed or lipemic specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: 6 months
Ordering

Performed

Tue, Thu, Sat

Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Reported

1-6 days

Synonyms

  • POSACONAZOLE
  • LAB8775

Additional Technical Information

Ordering Recommendations

Optimize drug therapy and monitor patient adherence.
Result Interpretation

Reference Interval

Effective August 15, 2011

Therapeutic Range (trough): Greater than 0.7 µg/mL

Interpretive Data

Adverse effects may include fever, nausea, vomiting, diarrhea, cardiac arrhythmias and liver toxicity.

Administrative

CPT Codes

80299

POTASSIUM WHOLE BLOOD

LAB3025

 

Collect

Plastic Arterial blood gas syringe with dry balanced heparin anticoagulant. Also acceptable: One Plain green top tube (sodium or lithium heparin) filled to at least 75% capacity.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; sample submitted with needle attached; air bubbles in sample; clotted sample; green top tube underfilled.

Storage/Transport Temperature

Internal: Deliver to laboratory immediately.

Offsite: Transport to lab at ambient temperature if within 30 minutes of collection. Transport refrigerated or on ice within one hour of collection.

Stability (from collection to initiation)

Ambient: 30 minutes; Refrigerated: 1 hour.

Performed by

PCL Clinical Laboratory

Performed

Upon receipt

Methodology

Ion Selective Electrode

Reported

Within 1 hour of receipt

Synonyms

  • LAB3025

Reference Interval

3.5-4.8 nmol/L

Performed by

PCL Clinical Laboratory

CPT Codes

84132

Collection

LAB3025

 

Collect

Plastic Arterial blood gas syringe with dry balanced heparin anticoagulant. Also acceptable: One Plain green top tube (sodium or lithium heparin) filled to at least 75% capacity.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; sample submitted with needle attached; air bubbles in sample; clotted sample; green top tube underfilled.

Storage/Transport Temperature

Internal: Deliver to laboratory immediately.

Offsite: Transport to lab at ambient temperature if within 30 minutes of collection. Transport refrigerated or on ice within one hour of collection.

Stability (from collection to initiation)

Ambient: 30 minutes; Refrigerated: 1 hour.

Performed by

PCL Clinical Laboratory

Ordering

Performed

Upon receipt

Methodology

Ion Selective Electrode

Reported

Within 1 hour of receipt

Synonyms

  • LAB3025
Result Interpretation

Reference Interval

3.5-4.8 nmol/L

Performed by

PCL Clinical Laboratory

Administrative

CPT Codes

84132

POTASSIUM, 24 HOUR URINE

LAB4843

Collect

Timed urine collection in plastic container.
Mandatory collection information: Start date and time and End date and time of collection.
 

Pediatric Collection

Timed urine collection in plastic container. Minimum volume 0.2 mL urine.
Mandatory collection information: Start date and time and End date and time of collection.
 

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.

Offsite: Transport to laboratory at ambient temperature.

Stability (from collection to initiation)

Ambient: 1 day; Refrigerated: 7 days; Frozen (-20 degrees C): 6 months.

Remarks

Requisition must state date and collection period (start time and end time). Indicate total volume when submitting an aliquot. Refrigerate during collection.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Indirect ISE

Reported

Same day

Reference Interval

Potassium excretion: 25.0 - 125.0 mmol/D

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84133

Collection

LAB4843

Collect

Timed urine collection in plastic container.
Mandatory collection information: Start date and time and End date and time of collection.
 

Pediatric Collection

Timed urine collection in plastic container. Minimum volume 0.2 mL urine.
Mandatory collection information: Start date and time and End date and time of collection.
 

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.

Offsite: Transport to laboratory at ambient temperature.

Stability (from collection to initiation)

Ambient: 1 day; Refrigerated: 7 days; Frozen (-20 degrees C): 6 months.

Remarks

Requisition must state date and collection period (start time and end time). Indicate total volume when submitting an aliquot. Refrigerate during collection.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Indirect ISE

Reported

Same day

Result Interpretation

Reference Interval

Potassium excretion: 25.0 - 125.0 mmol/D

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84133

POTASSIUM, PLASMA

LAB114

 

Collect

One 4.5 mL green top with gel (PST), (lithium heparin plasma).

Pediatric Collection

At least 1.0 mL blood collected in microtainer, green top with gel (lithium heparin plasma) or other tube with heparin.

Unacceptable Conditions

Gross hemolysis; potassium EDTA anticoagulant; sample not properly identified; insufficient sample volume.

Stability (from collection to initiation)

Before centrifugation: 1 hour.

After centrifugation: Ambient, 8 hours; Refrigerated, 2 days

Plasma aliquot: Ambient, 8 hours; Refrigerated, 2 days; Frozen (-20 degrees C), 6 months.

Remarks

Hemolyzed samples will cause a false elvation in potassium values. In samples with gross hemolysis the potassium results may not be reported.

Potassium results from serum samples will be slightly higher than for plasma samples.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Indirect ISE

Synonyms

  • LAB114
  • K

Reference Interval

Effective 05/20/2014:

3.5 - 5.1 mmol/L

 

Before 05/20/2014:

Sex From Age To Age Normal Low Normal High Units
M/F 0 1D 3.5 6.0 mmol/L
M/F 1D 16 3.5 5.0 mmol/L
M/F 16 150 3.3 5.0 mmol/L

Interpretive Data

Adult reference range.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84132

Collection

LAB114

 

Collect

One 4.5 mL green top with gel (PST), (lithium heparin plasma).

Pediatric Collection

At least 1.0 mL blood collected in microtainer, green top with gel (lithium heparin plasma) or other tube with heparin.

Unacceptable Conditions

Gross hemolysis; potassium EDTA anticoagulant; sample not properly identified; insufficient sample volume.

Stability (from collection to initiation)

Before centrifugation: 1 hour.

After centrifugation: Ambient, 8 hours; Refrigerated, 2 days

Plasma aliquot: Ambient, 8 hours; Refrigerated, 2 days; Frozen (-20 degrees C), 6 months.

Remarks

Hemolyzed samples will cause a false elvation in potassium values. In samples with gross hemolysis the potassium results may not be reported.

Potassium results from serum samples will be slightly higher than for plasma samples.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Indirect ISE

Synonyms

  • LAB114
  • K
Result Interpretation

Reference Interval

Effective 05/20/2014:

3.5 - 5.1 mmol/L

 

Before 05/20/2014:

Sex From Age To Age Normal Low Normal High Units
M/F 0 1D 3.5 6.0 mmol/L
M/F 1D 16 3.5 5.0 mmol/L
M/F 16 150 3.3 5.0 mmol/L

Interpretive Data

Adult reference range.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84132

POTASSIUM, RANDOM URINE

LAB434

 

Collect

5 mL random urine in plastic container.

Pediatric Collection

0.2 mL urine.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab at ambient temperature.

Stability (from collection to initiation)

Ambient: 1 day; Refrigerated: 7 days; Frozen (-20 degrees C): 6 months.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Indirect ISE

Reported

Same day

Synonyms

  • LAB434
  • K

Interpretive Data

Reference ranges are available for 24-hour urine specimens.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84133

Collection

LAB434

 

Collect

5 mL random urine in plastic container.

Pediatric Collection

0.2 mL urine.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab at ambient temperature.

Stability (from collection to initiation)

Ambient: 1 day; Refrigerated: 7 days; Frozen (-20 degrees C): 6 months.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Indirect ISE

Reported

Same day

Synonyms

  • LAB434
  • K
Result Interpretation

Interpretive Data

Reference ranges are available for 24-hour urine specimens.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84133

POTASSIUM, TIMED URINE

LAB435

Collect

Timed urine collection in plastic container.
Mandatory collection information: Start date and time and End date and time of collection.
 

Pediatric Collection

Timed urine collection in plastic container. Minimum volume 0.2 mL urine.
Mandatory collection information: Start date and time and End date and time of collection.
 

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab at ambient temperature.

Stability (from collection to initiation)

Ambient: 1 day; Refrigerated: 7 days; Frozen: 6 months.

Remarks

Requisition must state date and collection period. Indicate total collection volume if submitting an aliquot. Refrigerate during collection.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Indirect ISE

Reported

Same day

Synonyms

  • LAB435
  • K

Reference Interval

Potassium excretion: 25.0 - 125.0 mmol/D

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84133

Collection

LAB435

Collect

Timed urine collection in plastic container.
Mandatory collection information: Start date and time and End date and time of collection.
 

Pediatric Collection

Timed urine collection in plastic container. Minimum volume 0.2 mL urine.
Mandatory collection information: Start date and time and End date and time of collection.
 

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab at ambient temperature.

Stability (from collection to initiation)

Ambient: 1 day; Refrigerated: 7 days; Frozen: 6 months.

Remarks

Requisition must state date and collection period. Indicate total collection volume if submitting an aliquot. Refrigerate during collection.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Indirect ISE

Reported

Same day

Synonyms

  • LAB435
  • K
Result Interpretation

Reference Interval

Potassium excretion: 25.0 - 125.0 mmol/D

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84133

PRA - HIGH DEFINITION

LAB3672

 

Collect

One 6.0 mL plain red top tube

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 2 mL serum Deliver to lab within 24 hours at room temperature.

Remarks

Indicate purpose for testing, e.g. organ transplant type, HLA-matched platelets. For post-transplant patients, please indicate anti-rejection drug therapies on request form as some may interfere with testing. Maintain specimen at room temperature.

For Donor Specific Antibody

Performed by

Clinimmune Labs

Methodology

Luminex PRA, including HLA Specificity Analysis using purified single HLA antigen beads.

Synonyms

  • High Definition Percent Reactive Antibody
  • High Definition PRA
  • Donor Specific Antibody
  • LAB3672
  • HDPRA

Reference Interval

0 - 100 %

Performed by

Clinimmune Labs

CPT Codes

86832, (add 86833 if ordered as STAT-24 hours or CRITICAL)

Collection

LAB3672

 

Collect

One 6.0 mL plain red top tube

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 2 mL serum Deliver to lab within 24 hours at room temperature.

Remarks

Indicate purpose for testing, e.g. organ transplant type, HLA-matched platelets. For post-transplant patients, please indicate anti-rejection drug therapies on request form as some may interfere with testing. Maintain specimen at room temperature.

For Donor Specific Antibody

Performed by

Clinimmune Labs

Ordering

Methodology

Luminex PRA, including HLA Specificity Analysis using purified single HLA antigen beads.

Synonyms

  • High Definition Percent Reactive Antibody
  • High Definition PRA
  • Donor Specific Antibody
  • LAB3672
  • HDPRA
Result Interpretation

Reference Interval

0 - 100 %

Performed by

Clinimmune Labs

Administrative

CPT Codes

86832, (add 86833 if ordered as STAT-24 hours or CRITICAL)

More Articles...