PARVOVIRUS B19 ANTIBODY, IGM

LAB3149

 

Collect

Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, hyperlipemic, or icteric serum.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-3 days

Synonyms

  • Fifth Disease
  • LAB3149
  • PARVO M
  • PARVB19M

Ordering Recommendations

Not recommended as a stand-alone test. Parvovirus B19 Antibodies, IgG and IgM (0065120) is preferred.

Reference Interval

0.89 IV or less: Negative - No significant level of detectable Parvovirus B19 IgM antibody.
0.90-1.10 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.11 IV or greater: Positive - IgM antibody to Parvovirus B19 detected, which may indicate a current or recent infection.  However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Interpretive Data

The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

Appearance of an IgM antibody response normally occurs 7 to 14 days after the onset of disease. Testing immediately post-exposure is of no value without a later convalescent specimen. A residual IgM response may be distinguished from early IgM response to infection by testing sera from patients three to four weeks later for changing levels of specific IgM antibodies.

CPT Codes

86747
Collection

LAB3149

 

Collect

Serum separator tube. Also acceptable: Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, hyperlipemic, or icteric serum.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-3 days

Synonyms

  • Fifth Disease
  • LAB3149
  • PARVO M
  • PARVB19M

Ordering Recommendations

Not recommended as a stand-alone test. Parvovirus B19 Antibodies, IgG and IgM (0065120) is preferred.
Result Interpretation

Reference Interval

0.89 IV or less: Negative - No significant level of detectable Parvovirus B19 IgM antibody.
0.90-1.10 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.11 IV or greater: Positive - IgM antibody to Parvovirus B19 detected, which may indicate a current or recent infection.  However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

Interpretive Data

The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

Appearance of an IgM antibody response normally occurs 7 to 14 days after the onset of disease. Testing immediately post-exposure is of no value without a later convalescent specimen. A residual IgM response may be distinguished from early IgM response to infection by testing sera from patients three to four weeks later for changing levels of specific IgM antibodies.

Administrative

CPT Codes

86747

PARVOVIRUS B19 PCR PLASMA

LAB3256
PARVOPCRPLS

 

Collect

One 6.0 mL, pink top, plasma (EDTA), filled to at least 80% capacity.

Specimen source must be identified on order or requisition.

Pediatric Collection

At least 0.5 mL of whole blood collected in a 1.0 mL pink top microtainer (EDTA) or other blood tube with EDTA anticoagulant.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; contaminated specimen; clotted sample; tubes not filled to 80-100% capacity; improper storage conditions; gross hemolysis; no fixatives allowed; specimens other than plasma (EDTA).

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if tested within 72 hours. If delivery is not expected within 72 hours, centrifuge, transfer supernatant to an aliquot tube, and freeze at -70 degrees C. Ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours, Refrigerated: 72 hours, Frozen (-70 degrees C): 6 months.

Remarks

Qualitative results will be given.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Performed

2 times per week

Methodology

DNA amplification by polymerase chain reaction (PCR) with gel-electrophoresis of PCR products

Reported

Varies

Synonyms

  • LAB3256
  • PARVOPCRPLS

Interpretive Data

Not Detected (negative)

Detected (positive)

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

CPT Codes

87798

Collection

LAB3256
PARVOPCRPLS

 

Collect

One 6.0 mL, pink top, plasma (EDTA), filled to at least 80% capacity.

Specimen source must be identified on order or requisition.

Pediatric Collection

At least 0.5 mL of whole blood collected in a 1.0 mL pink top microtainer (EDTA) or other blood tube with EDTA anticoagulant.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; contaminated specimen; clotted sample; tubes not filled to 80-100% capacity; improper storage conditions; gross hemolysis; no fixatives allowed; specimens other than plasma (EDTA).

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if tested within 72 hours. If delivery is not expected within 72 hours, centrifuge, transfer supernatant to an aliquot tube, and freeze at -70 degrees C. Ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours, Refrigerated: 72 hours, Frozen (-70 degrees C): 6 months.

Remarks

Qualitative results will be given.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Ordering

Performed

2 times per week

Methodology

DNA amplification by polymerase chain reaction (PCR) with gel-electrophoresis of PCR products

Reported

Varies

Synonyms

  • LAB3256
  • PARVOPCRPLS
Result Interpretation

Interpretive Data

Not Detected (negative)

Detected (positive)

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Administrative

CPT Codes

87798

PARVOVIRUS B19 QUAL BY PCR

LAB3257
PARVOPCR
 

 

Collect

All sample types, except blood. Submit in a sterile container.

Specimen source must be identified on order or requisition.

If submitting a Pink Top tube (EDTA) whole blood, refer to test code LAB3256 PARVOVIRUS B19 PCR PLASMA.

Pediatric Collection

0.5 mL

Unacceptable Conditions

Sample not properly identified; insufficient sample volume; contaminated specimen; clotted sample; gross hemolysis; non sterile or leaking containers; and samples containing fixatives.

For plasma, refer to test code LAB3256 PARVOVIRUS B19 PCR PLASMA.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if delivery is less than 72 hours. If delivery is not expected within 72 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Ambient:  12 hours; Refrigerated: 72 hours; Frozen (-70 degrees C): 6 months

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Performed

2 times per week

Methodology

DNA amplification by polymerase chain reaction (PCR) with gel-electrophoresis of PCR products.

Reported

Varies

Synonyms

  • LAB3257
  • PARVOPCR

Interpretive Data

Not Detected (negative)

Detected (positive)

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

CPT Codes

87798

Collection

LAB3257
PARVOPCR
 

 

Collect

All sample types, except blood. Submit in a sterile container.

Specimen source must be identified on order or requisition.

If submitting a Pink Top tube (EDTA) whole blood, refer to test code LAB3256 PARVOVIRUS B19 PCR PLASMA.

Pediatric Collection

0.5 mL

Unacceptable Conditions

Sample not properly identified; insufficient sample volume; contaminated specimen; clotted sample; gross hemolysis; non sterile or leaking containers; and samples containing fixatives.

For plasma, refer to test code LAB3256 PARVOVIRUS B19 PCR PLASMA.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if delivery is less than 72 hours. If delivery is not expected within 72 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Ambient:  12 hours; Refrigerated: 72 hours; Frozen (-70 degrees C): 6 months

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Ordering

Performed

2 times per week

Methodology

DNA amplification by polymerase chain reaction (PCR) with gel-electrophoresis of PCR products.

Reported

Varies

Synonyms

  • LAB3257
  • PARVOPCR
Result Interpretation

Interpretive Data

Not Detected (negative)

Detected (positive)

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Administrative

CPT Codes

87798

PENTOBARBITAL, BLOOD

LAB3600

 

Collect

One 6 mL red top tube, no gel, clot activator serum.

Pediatric Collection

Minimum volume: 1 mL serum.

Unacceptable Conditions

SST or PST tubes; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Allow to clot for 30 minutes prior to centrifuging. Centrifuge within 24 hours of collection. Place at least 1 mL serum/plasma in an aliquot container and transport to lab at 2-8 degrees C (refrigerated or on ice).

Stability (from collection to initiation)

Prior to centrifugation: Ambient, 24 hours.

Serum aliquot: Ambient, 24 hours; Refrigerated (2-8 degrees C), 1 week; Frozen (-20 degrees C), 1 month.

Remarks

The following drugs were found to cause interference:

  • Amobarbital
  • Aprobarbital
  • Butalbital
  • Secobarbital
  • Thiopental

Note: Not all barbiturates have been evaluated for interference.

Performed by

PCL Clinical Laboratory - Toxicology

Performed

Daily. Samples must be received in lab by 11:00 am for same day testing.

Methodology

Gas Chromatography-Mass Spectrometry

Reported

Within 24 hours

Synonyms

  • -313
  • LAB3600
  • Nembutal
  • Embutal

Reference Interval

Induced Coma/Intracranial Pressure Control.........30-50 ug/mL

Performed by

PCL Clinical Laboratory - Toxicology

CPT Codes

82205

Collection

LAB3600

 

Collect

One 6 mL red top tube, no gel, clot activator serum.

Pediatric Collection

Minimum volume: 1 mL serum.

Unacceptable Conditions

SST or PST tubes; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Allow to clot for 30 minutes prior to centrifuging. Centrifuge within 24 hours of collection. Place at least 1 mL serum/plasma in an aliquot container and transport to lab at 2-8 degrees C (refrigerated or on ice).

Stability (from collection to initiation)

Prior to centrifugation: Ambient, 24 hours.

Serum aliquot: Ambient, 24 hours; Refrigerated (2-8 degrees C), 1 week; Frozen (-20 degrees C), 1 month.

Remarks

The following drugs were found to cause interference:

  • Amobarbital
  • Aprobarbital
  • Butalbital
  • Secobarbital
  • Thiopental

Note: Not all barbiturates have been evaluated for interference.

Performed by

PCL Clinical Laboratory - Toxicology

Ordering

Performed

Daily. Samples must be received in lab by 11:00 am for same day testing.

Methodology

Gas Chromatography-Mass Spectrometry

Reported

Within 24 hours

Synonyms

  • -313
  • LAB3600
  • Nembutal
  • Embutal
Result Interpretation

Reference Interval

Induced Coma/Intracranial Pressure Control.........30-50 ug/mL

Performed by

PCL Clinical Laboratory - Toxicology

Administrative

CPT Codes

82205

PERCENT REACTIVE ANTIBODY

LAB3643

 

Collect

10 mL plain red

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 2 mL serum Deliver to lab within 24 hours at room temperature.

Stability (from collection to initiation)

.

Remarks

Indicate purpose for testing, e.g. organ transplant type, HLA-matched platelets. For post-transplant patients, please indicate anti-rejection drug therapies on request form as some may interfere with testing. Maintain specimen at room temperature.

 

HD-PRA used for Donor Specific Antibody

Performed by

Clinimmune Labs

Methodology

Luminex xMAP technology detects HLA class I and class II IgG antibodies using microspheres coated with purified HLA class I and class II antigens.

 

Note:  HD-PRA uses purified single HLA antigen beads.

Synonyms

  • DONOR SPECIFIC ANTIBODY
  • LAB3643
  • PRA
  • PERCENT

Reference Interval

0-100%

or

Negative, Weak Positive or Positive

Performed by

Clinimmune Labs

CPT Codes

86807

Collection

LAB3643

 

Collect

10 mL plain red

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 2 mL serum Deliver to lab within 24 hours at room temperature.

Stability (from collection to initiation)

.

Remarks

Indicate purpose for testing, e.g. organ transplant type, HLA-matched platelets. For post-transplant patients, please indicate anti-rejection drug therapies on request form as some may interfere with testing. Maintain specimen at room temperature.

 

HD-PRA used for Donor Specific Antibody

Performed by

Clinimmune Labs

Ordering

Methodology

Luminex xMAP technology detects HLA class I and class II IgG antibodies using microspheres coated with purified HLA class I and class II antigens.

 

Note:  HD-PRA uses purified single HLA antigen beads.

Synonyms

  • DONOR SPECIFIC ANTIBODY
  • LAB3643
  • PRA
  • PERCENT
Result Interpretation

Reference Interval

0-100%

or

Negative, Weak Positive or Positive

Performed by

Clinimmune Labs

Administrative

CPT Codes

86807

PERTUSSIS PCR - QUALITATIVE

LAB923

Collect

Respiratory specimen: Aspirate, bronchoalveolar lavage (BAL), swab or sputum.

Specimen Preparation

Fluid: Transfer 2 ml respiratory specimen to sterile container. (Min: 0.5 ml) Also acceptable: Transfer to viral transport m media (ARUP supply #12884) available online through eSupply using ARUP Connectâ„¢ or contact ARUP Client Services at (800) 522-2787.
Swabs:
Place in viral transport media.

Unacceptable Conditions

Calcium-alginate swabs.

Storage/Transport Temperature

Frozen

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 5 days; Frozen: 2 weeks

Performed

Sun-Sat

Methodology

Qualitative Polymerase Chain Reaction

Reported

1-4 days

Synonyms

  • LAB923
  • B pertussis PCR
  • Bordetella pertussis PCR
  • PERTUSSPCR

Ordering Recommendations

CDC-recommended test for the diagnosis of pertussis.

CPT Codes

87798
Collection

LAB923

Collect

Respiratory specimen: Aspirate, bronchoalveolar lavage (BAL), swab or sputum.

Specimen Preparation

Fluid: Transfer 2 ml respiratory specimen to sterile container. (Min: 0.5 ml) Also acceptable: Transfer to viral transport m media (ARUP supply #12884) available online through eSupply using ARUP Connectâ„¢ or contact ARUP Client Services at (800) 522-2787.
Swabs:
Place in viral transport media.

Unacceptable Conditions

Calcium-alginate swabs.

Storage/Transport Temperature

Frozen

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 5 days; Frozen: 2 weeks
Ordering

Performed

Sun-Sat

Methodology

Qualitative Polymerase Chain Reaction

Reported

1-4 days

Synonyms

  • LAB923
  • B pertussis PCR
  • Bordetella pertussis PCR
  • PERTUSSPCR

Ordering Recommendations

CDC-recommended test for the diagnosis of pertussis.
Result Interpretation
Administrative

CPT Codes

87798

PH ARTERIAL

LAB4525

PH ARTERIAL

 

Collect

At least 1.0 mL arterial whole blood collected in plastic blood gas syringe with dry balanced heparin anticoagulant.

Pediatric Collection

0.3 mL arterial whole blood in plastic tuberculin blood gas syringe with dry balanced heparin.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; sample submitted with needle attached (remove needle immediately post-collection); uncapped sample syringe; sample with air bubbles; clotted sample; EDTA or citrate anticoagulant.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature. If sample cannot be submitted to the lab within 30 minutes of collection, transport on cold pack.

Offsite: Utilize emergency courier to provide for transport criteria listed above.

Stability (from collection to initiation)

Ambient: 30 minutes; Refrigerated: 1 hour.

Performed by

PCL Clinical Laboratory - Core Rapid Room

Performed

Upon receipt

Methodology

Ion-selective electrode

Reported

Same day

Reference Interval

7.350-7.450

Performed by

PCL Clinical Laboratory - Core Rapid Room

CPT Codes

82800
Collection

LAB4525

PH ARTERIAL

 

Collect

At least 1.0 mL arterial whole blood collected in plastic blood gas syringe with dry balanced heparin anticoagulant.

Pediatric Collection

0.3 mL arterial whole blood in plastic tuberculin blood gas syringe with dry balanced heparin.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; sample submitted with needle attached (remove needle immediately post-collection); uncapped sample syringe; sample with air bubbles; clotted sample; EDTA or citrate anticoagulant.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature. If sample cannot be submitted to the lab within 30 minutes of collection, transport on cold pack.

Offsite: Utilize emergency courier to provide for transport criteria listed above.

Stability (from collection to initiation)

Ambient: 30 minutes; Refrigerated: 1 hour.

Performed by

PCL Clinical Laboratory - Core Rapid Room
Ordering

Performed

Upon receipt

Methodology

Ion-selective electrode

Reported

Same day
Result Interpretation

Reference Interval

7.350-7.450

Performed by

PCL Clinical Laboratory - Core Rapid Room
Administrative

CPT Codes

82800

PH BODY FLUID

LAB110

PH BF

 

Collect

1 mL fluid in sterile container

Pediatric Collection

1 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport refrigerated.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated 24 hours; Frozen: 3 months.

Remarks

Identify fluid source on requisition.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Ion-selective electrode (ISE)

Reported

Same day

Synonyms

  • LAB110
  • PH BF

Performed by

PCL Clinical Laboratory - Core

CPT Codes

83986

Collection

LAB110

PH BF

 

Collect

1 mL fluid in sterile container

Pediatric Collection

1 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport refrigerated.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated 24 hours; Frozen: 3 months.

Remarks

Identify fluid source on requisition.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Ion-selective electrode (ISE)

Reported

Same day

Synonyms

  • LAB110
  • PH BF
Result Interpretation

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

83986

PH URINE METER

LAB3287

URINE PH

Collect

5 mL urine in plastic container, no preservatives.

Pediatric Collection

1 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport refrigerated.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 24 hours

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Ion-selective electrode (ISE)

Reported

Same day

Synonyms

  • LAB3287

Reference Interval

4.50-8.00 Units

Performed by

PCL Clinical Laboratory - Core

CPT Codes

83986

Collection

LAB3287

URINE PH

Collect

5 mL urine in plastic container, no preservatives.

Pediatric Collection

1 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport refrigerated.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 24 hours

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Ion-selective electrode (ISE)

Reported

Same day

Synonyms

  • LAB3287
Result Interpretation

Reference Interval

4.50-8.00 Units

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

83986

PH VENOUS

LAB75

PH VEN C

Collect

At least 1.0 mL venous whole blood collected in a plastic blood gas syringe with dry balanced heparin anticoagulant.

Pediatric Collection

0.3 mL venous whole blood in plastic tuberculin blood gas syringe with dry balanced heparin.

 

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; sample submitted with needle attached (remove needle immediately post-collection); uncapped sample syringe; sample with air bubbles; clotted sample; EDTA or citrate anticoagulant.

 

Stability (from collection to initiation)

Ambient: 30 minutes; Refrigerated: 1 hour.

Performed by

PCL Clinical Laboratory - Core Rapid Room

Performed

Upon receipt

Methodology

Ion-selective electrode

Reported

Same day

Reference Interval

7.330 - 7.430

Performed by

PCL Clinical Laboratory - Core Rapid Room

CPT Codes

82800
Collection

LAB75

PH VEN C

Collect

At least 1.0 mL venous whole blood collected in a plastic blood gas syringe with dry balanced heparin anticoagulant.

Pediatric Collection

0.3 mL venous whole blood in plastic tuberculin blood gas syringe with dry balanced heparin.

 

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; sample submitted with needle attached (remove needle immediately post-collection); uncapped sample syringe; sample with air bubbles; clotted sample; EDTA or citrate anticoagulant.

 

Stability (from collection to initiation)

Ambient: 30 minutes; Refrigerated: 1 hour.

Performed by

PCL Clinical Laboratory - Core Rapid Room

Ordering

Performed

Upon receipt

Methodology

Ion-selective electrode

Reported

Same day
Result Interpretation

Reference Interval

7.330 - 7.430

Performed by

PCL Clinical Laboratory - Core Rapid Room

Administrative

CPT Codes

82800

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