Tests Catalogs

PHOSPHORUS, RANDOM URINE

LAB427

 

Collect

5 mL random urine in plastic container, no preservative.

Pediatric Collection

0.2 mL minimum volume.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab at ambient temperature.

Stability (from collection to initiation)

Ambient: 6 hours; Refrigerated: 2 days; Frozen (-20 degrees C): 6 months.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Colorimetry

Reported

Same day

Synonyms

  • LAB427
  • PO4

Interpretive Data

Reference ranges are available for 24-hour urine specimens.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84105

Collection

LAB427

 

Collect

5 mL random urine in plastic container, no preservative.

Pediatric Collection

0.2 mL minimum volume.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab at ambient temperature.

Stability (from collection to initiation)

Ambient: 6 hours; Refrigerated: 2 days; Frozen (-20 degrees C): 6 months.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Colorimetry

Reported

Same day

Synonyms

  • LAB427
  • PO4
Result Interpretation

Interpretive Data

Reference ranges are available for 24-hour urine specimens.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84105

PHOSPHORUS, SERUM/PLASMA

LAB113

 

Collect

One 4.5 mL Green top with gel (PST), (lithium heparin plasma).

Pediatric Collection

At least 0.5 mL whole blood collected in a microtainer, green top with gel, lithium heparin plasma.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection. Transport to laboratory at ambient temperature.

Stability (from collection to initiation)

Before centrifugation: Ambient, 1 hour.

After centrifugation: Ambient, 3 days; Refrigerated, 7 days; Frozen, Unacceptable.

Plasma aliquot: Ambient, 3 days; Refrigerated, 7 days; Frozen (-20 degrees C),  2 months.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Colorimetry.

Reported

Same day

Synonyms

  • LAB113
  • PO4

Reference Interval

Effective 05/20/2014:

2.5 - 5 mg/dL

 

Befor 05/20/2014:

Sex From Age To Age Normal Low Normal High Units
M/F 0 1M 4.2 9.0 mg/dL
M/F 1M 1 3.8 6.2 mg/dL
M/F 1 5 3.5 6.8 mg/dL
M/F 5 150 2.7 4.5 mg/dL

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84100

Collection

LAB113

 

Collect

One 4.5 mL Green top with gel (PST), (lithium heparin plasma).

Pediatric Collection

At least 0.5 mL whole blood collected in a microtainer, green top with gel, lithium heparin plasma.

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection. Transport to laboratory at ambient temperature.

Stability (from collection to initiation)

Before centrifugation: Ambient, 1 hour.

After centrifugation: Ambient, 3 days; Refrigerated, 7 days; Frozen, Unacceptable.

Plasma aliquot: Ambient, 3 days; Refrigerated, 7 days; Frozen (-20 degrees C),  2 months.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Colorimetry.

Reported

Same day

Synonyms

  • LAB113
  • PO4
Result Interpretation

Reference Interval

Effective 05/20/2014:

2.5 - 5 mg/dL

 

Befor 05/20/2014:

Sex From Age To Age Normal Low Normal High Units
M/F 0 1M 4.2 9.0 mg/dL
M/F 1M 1 3.8 6.2 mg/dL
M/F 1 5 3.5 6.8 mg/dL
M/F 5 150 2.7 4.5 mg/dL

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84100

PHOSPHORUS, TIMED URINE

LAB426

 

Collect

Timed urine collection in plastic container.
Mandatory collection information: Start date and time and End date and time of collection.
 

Pediatric Collection

Timed urine collection in plastic container. Minimum volume 0.2 mL urine.
Mandatory collection information: Start date and time and End date and time of collection.
 

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab at ambient temperature.

Stability (from collection to initiation)

Ambient: 6 hours; Refrigerated: 2 days; Frozen (-20 degrees C): 6 months.

Remarks

Requisition must state date and collection period. Indicate total collection volume if submitting an aliquot. Refrigerate during collection.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Colorimetry

Reported

Same day

Synonyms

  • LAB426
  • PO4

Reference Interval

Effective 05/20/2014:

0.3 - 1.3 g/D

 

Before 05/20/2014:

Phosphorus excretion: 0.4 - 1.3 g/D

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84105

Collection

LAB426

 

Collect

Timed urine collection in plastic container.
Mandatory collection information: Start date and time and End date and time of collection.
 

Pediatric Collection

Timed urine collection in plastic container. Minimum volume 0.2 mL urine.
Mandatory collection information: Start date and time and End date and time of collection.
 

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport to lab at ambient temperature.

Stability (from collection to initiation)

Ambient: 6 hours; Refrigerated: 2 days; Frozen (-20 degrees C): 6 months.

Remarks

Requisition must state date and collection period. Indicate total collection volume if submitting an aliquot. Refrigerate during collection.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Colorimetry

Reported

Same day

Synonyms

  • LAB426
  • PO4
Result Interpretation

Reference Interval

Effective 05/20/2014:

0.3 - 1.3 g/D

 

Before 05/20/2014:

Phosphorus excretion: 0.4 - 1.3 g/D

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84105

PHYTANIC ACID

LAB3077

 

Collect

2 mL green top tube 2 mL red top tube with or without gel

Pediatric Collection

0.3 mL

Storage/Transport Temperature

Internal: Deliver to lab immediately at room temperature
Offsite: 0.5 mL plasma/serum. Ship heparinized plasma/serum frozen.

Performed by

UCD Biochemical Genetics Laboratory

Performed

Weekly (Dayshift), day varies

Reported

7 - 10 days

Synonyms

  • LAB3077
  • PHYTANIC

Performed by

UCD Biochemical Genetics Laboratory

CPT Codes

82543

Collection

LAB3077

 

Collect

2 mL green top tube 2 mL red top tube with or without gel

Pediatric Collection

0.3 mL

Storage/Transport Temperature

Internal: Deliver to lab immediately at room temperature
Offsite: 0.5 mL plasma/serum. Ship heparinized plasma/serum frozen.

Performed by

UCD Biochemical Genetics Laboratory

Ordering

Performed

Weekly (Dayshift), day varies

Reported

7 - 10 days

Synonyms

  • LAB3077
  • PHYTANIC
Result Interpretation

Performed by

UCD Biochemical Genetics Laboratory

Administrative

CPT Codes

82543

PINWORM PREP

LAB248

 

Collect

Scotch tape slide preparation of perianal region. Send slide in a covered container.

Unacceptable Conditions

Use of nontransparent Scotch tape, tape on both sides of the slide, specimen not inside a covered container, use of frosted slide, tape sent sticky side up.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: Transport immediately to the laboratory.

Remarks

Must use clear tape. Cloudy or magic Scotch Tape are unacceptable. CAUTION: Pinworm eggs are very infectious.

Performed by

PCL Clinical Laboratory - Microbiology

Performed

Daily

Methodology

Direct examination of Scotch tape slide preparation.

Reported

Varies

Synonyms

  • LAB248

Performed by

PCL Clinical Laboratory - Microbiology

CPT Codes

87172

Collection

LAB248

 

Collect

Scotch tape slide preparation of perianal region. Send slide in a covered container.

Unacceptable Conditions

Use of nontransparent Scotch tape, tape on both sides of the slide, specimen not inside a covered container, use of frosted slide, tape sent sticky side up.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: Transport immediately to the laboratory.

Remarks

Must use clear tape. Cloudy or magic Scotch Tape are unacceptable. CAUTION: Pinworm eggs are very infectious.

Performed by

PCL Clinical Laboratory - Microbiology

Ordering

Performed

Daily

Methodology

Direct examination of Scotch tape slide preparation.

Reported

Varies

Synonyms

  • LAB248
Result Interpretation

Performed by

PCL Clinical Laboratory - Microbiology

Administrative

CPT Codes

87172

PLASMA HEMOGLOBIN

LAB92

 

Collect

One 4.0 mL Purple top tube (EDTA).

Tube must be at least half full.

Pediatric Collection

Minimum volume: 0.5 mL EDTA plasma. Original collection tube, any size, must be at least half full.

Unacceptable Conditions

Specimen not properly identified; incorrect container; original container less than half full; insufficient sample volume; heelstick or fingerstick samples; frozen samples.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within one hour of collection. Place at least 0.5 mL plasma in aliquot container and deliver to lab refrigerated/on ice (2-8 degrees C).

Stability (from collection to initiation)

Before centrifugation: Refrigerated (2-8 degrees C), 1 hour.

Plasma aliquot: Ambient, 2 hours; Refrigerated (2-8 degrees C), 1 week; Frozen, Unacceptable.

Remarks

Whole blood must be spun within one hour of draw. Centrifuge and place plasma in aliquot container if delivery to lab is delayed.

Performed by

PCL Clinical Laboratory - Toxicology

Performed

Daily

Methodology

Spectrophotometric

Reported

Daily

Synonyms

  • Plasma Free Hemoglobin
  • LAB92

Reference Interval

Normal collection : 0 - 10 mg/dL

Interpretive Data

This test is not reliable when serum bilirubin is greater than 1.0 mg/dL.

Performed by

PCL Clinical Laboratory - Toxicology

CPT Codes

83051

Collection

LAB92

 

Collect

One 4.0 mL Purple top tube (EDTA).

Tube must be at least half full.

Pediatric Collection

Minimum volume: 0.5 mL EDTA plasma. Original collection tube, any size, must be at least half full.

Unacceptable Conditions

Specimen not properly identified; incorrect container; original container less than half full; insufficient sample volume; heelstick or fingerstick samples; frozen samples.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within one hour of collection. Place at least 0.5 mL plasma in aliquot container and deliver to lab refrigerated/on ice (2-8 degrees C).

Stability (from collection to initiation)

Before centrifugation: Refrigerated (2-8 degrees C), 1 hour.

Plasma aliquot: Ambient, 2 hours; Refrigerated (2-8 degrees C), 1 week; Frozen, Unacceptable.

Remarks

Whole blood must be spun within one hour of draw. Centrifuge and place plasma in aliquot container if delivery to lab is delayed.

Performed by

PCL Clinical Laboratory - Toxicology

Ordering

Performed

Daily

Methodology

Spectrophotometric

Reported

Daily

Synonyms

  • Plasma Free Hemoglobin
  • LAB92
Result Interpretation

Reference Interval

Normal collection : 0 - 10 mg/dL

Interpretive Data

This test is not reliable when serum bilirubin is greater than 1.0 mg/dL.

Performed by

PCL Clinical Laboratory - Toxicology

Administrative

CPT Codes

83051

PLASMINOGEN ACTIVITY

LAB847

 

Collect

One 2.7 mL Blue top tube (sodium citrate 3.2%). Fill tube to at least 90% capacity.

All blue tops collected for coagulation assays which do not have a blood culture collected first must have a discard tube collected prior to filling. Discard tube should be another blue top in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.3 mL platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample diluted/contaminated with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma and store in plastic container. Transport plasma frozen.

Stability (from collection to initiation)

Whole blood: Ambient temperature, 4 hours.

Platelet poor plasma: Refrigerated (2-4 degrees C), 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70 degrees C), 6 months.

Remarks

Reference range is established using 3.2% sodium citrate. Patient history is helpful for interpretation of result.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Performed

Monday through Friday, day shift only

Methodology

Chromogenic assay

Reported

Same day

Synonyms

  • LAB847

Reference Interval

Effective 01/11/2016:

72 - 121%

Before 01/11/2016:

75.0 - 128.0 U/dL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

CPT Codes

85420

Collection

LAB847

 

Collect

One 2.7 mL Blue top tube (sodium citrate 3.2%). Fill tube to at least 90% capacity.

All blue tops collected for coagulation assays which do not have a blood culture collected first must have a discard tube collected prior to filling. Discard tube should be another blue top in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.3 mL platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample diluted/contaminated with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma and store in plastic container. Transport plasma frozen.

Stability (from collection to initiation)

Whole blood: Ambient temperature, 4 hours.

Platelet poor plasma: Refrigerated (2-4 degrees C), 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70 degrees C), 6 months.

Remarks

Reference range is established using 3.2% sodium citrate. Patient history is helpful for interpretation of result.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Ordering

Performed

Monday through Friday, day shift only

Methodology

Chromogenic assay

Reported

Same day

Synonyms

  • LAB847
Result Interpretation

Reference Interval

Effective 01/11/2016:

72 - 121%

Before 01/11/2016:

75.0 - 128.0 U/dL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Administrative

CPT Codes

85420

PLATELET AGGREGATION

LAB3065

PLT AGG

Collect

Four full 3.0 mL Blue top tubes (3.2% Na Citrate) and One 5 mL Purple top tube (EDTA).  Do not centrifuge.

Patient Preparation

  1. Patients must call the laboratory two weeks in advance to schedule an appointment at 720-848-6938.
  2. The patient should be fasting before sample collection.
  3. Patients must not take any anti-platelet medication or NSAIDS in the 14 day period prior to sample collection.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; Tubes filled improperly (less than 90% full or greater than 110% full); sample centrifuged; sample refrigerated; plasma; platelet count under 100,000; patients taking NSAIDs or anti-platelet medication; frozen samples; centrifuged samples; samples sent in the pneumatic tube system.

Storage/Transport Temperature

Internal:   Deliver to laboratory immediately at ambient temperature. Specimens must be hand-carried. Do not use pneumatic tube system.

Offsite:  Must be received within 1.0 hours of collection time. Maintain specimens at ambient temperature at all times.

Stability (from collection to initiation)

Ambient: 1.0 hours.

Do not refrigerate or freeze.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Performed

By appointment only.  Performed Monday and Wednesday.

Call 720-848-6938 for an appointment.

Methodology

Chrono-log Whole Blood Platelet Aggregation by impedance method with ATP secretion luminescence.

Reported

The test is reported the same day and the interpretation will follow.

Synonyms

  • PLTAGG
  • Platelet Function Studies

Reference Interval

Platelet Aggregation/Secretion Agonist Reference Range
Thrombin 1 Unit secretion >0.5 nmoles
ADP 10 uM aggregation 10 - 29 Ohms
ADP 10 uM secretion 0 - 1.51 nmoles
COLLAGEN 1 ug/mL aggregation 10 - 24 Ohms
COLLAGEN 1 ug/mL secretion 0.5 - 1.8 nmoles
COLLAGEN 5 ug/mL aggregation 9 - 35 Ohms
COLLAGEN 5 ug/mL secretion 0.8 - 2.3 nmoles
ARACHIDONIC ACID 0.5 uM aggregation 8 - 26 Ohms
ARACHIDONIC ACID 0.5 uM secretion 0.6 - 2.4 nmoles
RISTOCETIN LOW 0.25 mg/mL aggregation < or = to 5 Ohms
RISTOCETIN HIGH 1.0 mg/mL aggregation >5 Ohms
 

Interpretive Data

Interpretation supplied by medical director.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

CPT Codes

85576 x 11; 85049 (platelet count)

Collection

LAB3065

PLT AGG

Collect

Four full 3.0 mL Blue top tubes (3.2% Na Citrate) and One 5 mL Purple top tube (EDTA).  Do not centrifuge.

Patient Preparation

  1. Patients must call the laboratory two weeks in advance to schedule an appointment at 720-848-6938.
  2. The patient should be fasting before sample collection.
  3. Patients must not take any anti-platelet medication or NSAIDS in the 14 day period prior to sample collection.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; Tubes filled improperly (less than 90% full or greater than 110% full); sample centrifuged; sample refrigerated; plasma; platelet count under 100,000; patients taking NSAIDs or anti-platelet medication; frozen samples; centrifuged samples; samples sent in the pneumatic tube system.

Storage/Transport Temperature

Internal:   Deliver to laboratory immediately at ambient temperature. Specimens must be hand-carried. Do not use pneumatic tube system.

Offsite:  Must be received within 1.0 hours of collection time. Maintain specimens at ambient temperature at all times.

Stability (from collection to initiation)

Ambient: 1.0 hours.

Do not refrigerate or freeze.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Ordering

Performed

By appointment only.  Performed Monday and Wednesday.

Call 720-848-6938 for an appointment.

Methodology

Chrono-log Whole Blood Platelet Aggregation by impedance method with ATP secretion luminescence.

Reported

The test is reported the same day and the interpretation will follow.

Synonyms

  • PLTAGG
  • Platelet Function Studies
Result Interpretation

Reference Interval

Platelet Aggregation/Secretion Agonist Reference Range
Thrombin 1 Unit secretion >0.5 nmoles
ADP 10 uM aggregation 10 - 29 Ohms
ADP 10 uM secretion 0 - 1.51 nmoles
COLLAGEN 1 ug/mL aggregation 10 - 24 Ohms
COLLAGEN 1 ug/mL secretion 0.5 - 1.8 nmoles
COLLAGEN 5 ug/mL aggregation 9 - 35 Ohms
COLLAGEN 5 ug/mL secretion 0.8 - 2.3 nmoles
ARACHIDONIC ACID 0.5 uM aggregation 8 - 26 Ohms
ARACHIDONIC ACID 0.5 uM secretion 0.6 - 2.4 nmoles
RISTOCETIN LOW 0.25 mg/mL aggregation < or = to 5 Ohms
RISTOCETIN HIGH 1.0 mg/mL aggregation >5 Ohms
 

Interpretive Data

Interpretation supplied by medical director.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Administrative

CPT Codes

85576 x 11; 85049 (platelet count)

PLATELET ANTIBODIES - INDIRECT (Equivalent to PAK PLUS/PAK-12)

LAB767

 

Collect

Serum separator tube (SST).

Patient Preparation

For newborns less than 30 days old, collect specimen from the mother.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube and freeze. (Min: 0.5 mL)

Unacceptable Conditions

Microbially contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Storage/Transport Temperature

Frozen. Also acceptable: Refrigerated if maintained at temperature for 48 hours or less.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 1 month

Notes

Further characterization of antibodies directed to platelet glycoproteins IIb/IIIa may be performed at client request by ordering Platelet Antibody Identification (0051051).

Performed

Mon-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-3 days

Synonyms

  • PAK Plus Antibodies
  • LAB767
  • PLATABIND

Ordering Recommendations

Not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). Use to detect platelet-specific antibodies in suspected fetal or neonatal alloimmune thrombocytopenia, posttransfusion purpura, or multiplatelet transfusion refractoriness. Preferred test is Platelet Antibodies, Indirect with Reflex to Identification (0051718).

Reference Interval

None detected.

Interpretive Data

This is the primary test for detection of platelet-specific antibodies. It is not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). This test will detect both allo and autoantibodies, but will not distinguish between them. Results of this test should be used in conjunction with clinical findings and other serological tests. Antibodies directed to antigens found on platelets are associated with many different clinical situations. Immune thrombocytopenic purpura (ITP) is a destructive thrombocytopenia caused by autoantibodies. Neonatal alloimmune thrombocytopenia (NATP) and post-transfusion purpura (PTP) are diseases where thrombocytopenia is caused by platelet-specific alloantibodies. HLA alloantibodies do not cause thrombocytopenia, but are commonly associated with refractoriness to platelet transfusions.

This test is designed to detect antibodies to platelet glycoproteins IIb/IIIa (HPA-1a/1b [PlA1 and PlA2], HPA-3a/3b, and HPA-4a), Ia/IIA (HPA-5a/5b), Ib/IX, and IV. In addition, this test will also detect antibodies to HLA Class I antigens (HLA-A-B).

Testing for neonatal alloimmune thrombocytopenia should be performed using a maternal serum, since platelet antibody may not be detected in a neonatal serum. False negative results are common in infant samples.

CPT Codes

86022
Collection

LAB767

 

Collect

Serum separator tube (SST).

Patient Preparation

For newborns less than 30 days old, collect specimen from the mother.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube and freeze. (Min: 0.5 mL)

Unacceptable Conditions

Microbially contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Storage/Transport Temperature

Frozen. Also acceptable: Refrigerated if maintained at temperature for 48 hours or less.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 1 month

Notes

Further characterization of antibodies directed to platelet glycoproteins IIb/IIIa may be performed at client request by ordering Platelet Antibody Identification (0051051).
Ordering

Performed

Mon-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-3 days

Synonyms

  • PAK Plus Antibodies
  • LAB767
  • PLATABIND

Ordering Recommendations

Not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). Use to detect platelet-specific antibodies in suspected fetal or neonatal alloimmune thrombocytopenia, posttransfusion purpura, or multiplatelet transfusion refractoriness. Preferred test is Platelet Antibodies, Indirect with Reflex to Identification (0051718).
Result Interpretation

Reference Interval

None detected.

Interpretive Data

This is the primary test for detection of platelet-specific antibodies. It is not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). This test will detect both allo and autoantibodies, but will not distinguish between them. Results of this test should be used in conjunction with clinical findings and other serological tests. Antibodies directed to antigens found on platelets are associated with many different clinical situations. Immune thrombocytopenic purpura (ITP) is a destructive thrombocytopenia caused by autoantibodies. Neonatal alloimmune thrombocytopenia (NATP) and post-transfusion purpura (PTP) are diseases where thrombocytopenia is caused by platelet-specific alloantibodies. HLA alloantibodies do not cause thrombocytopenia, but are commonly associated with refractoriness to platelet transfusions.

This test is designed to detect antibodies to platelet glycoproteins IIb/IIIa (HPA-1a/1b [PlA1 and PlA2], HPA-3a/3b, and HPA-4a), Ia/IIA (HPA-5a/5b), Ib/IX, and IV. In addition, this test will also detect antibodies to HLA Class I antigens (HLA-A-B).

Testing for neonatal alloimmune thrombocytopenia should be performed using a maternal serum, since platelet antibody may not be detected in a neonatal serum. False negative results are common in infant samples.

Administrative

CPT Codes

86022

PLATELET COUNT

LAB301

PLT

 

Collect

One 4 mL Purple top (EDTA whole blood).  Minimum volume 1.0 mL.

Pediatric Collection

At least 0.5 mL whole blood in a 1.0 mL microtainer, purple top (EDTA whole blood).

Unacceptable Conditions

Specimen not properly identified; incorrect container; clotted sample; frozen sample.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Do not centrifuge. Transport at 2-8 degrees C if transport time will exceed 24 hours.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 36 hours; Frozen: Unacceptable.

Performed by

PCL Clinical Laboratory - Core

Notes

Blood volume of 0.5 mL or less in a 4.0 mL purple top (EDTA whole blood) is unacceptable. Blood volume between 0.5 mL and 1.0 mL in a 4.0 mL purple top (EDTA whole blood) is a suboptimal specimen and results will be reported with this disclaimer: "Low volume sample, results may be unreliable."

Performed

Daily

Methodology

Automated Cell Counter.

Reported

Routine requests are reported within 8 hours.

Synonyms

  • LAB301
  • PLT

Reference Interval

150 - 400 10^9/L

Performed by

PCL Clinical Laboratory - Core

CPT Codes

85049

Collection

LAB301

PLT

 

Collect

One 4 mL Purple top (EDTA whole blood).  Minimum volume 1.0 mL.

Pediatric Collection

At least 0.5 mL whole blood in a 1.0 mL microtainer, purple top (EDTA whole blood).

Unacceptable Conditions

Specimen not properly identified; incorrect container; clotted sample; frozen sample.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Do not centrifuge. Transport at 2-8 degrees C if transport time will exceed 24 hours.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 36 hours; Frozen: Unacceptable.

Performed by

PCL Clinical Laboratory - Core

Notes

Blood volume of 0.5 mL or less in a 4.0 mL purple top (EDTA whole blood) is unacceptable. Blood volume between 0.5 mL and 1.0 mL in a 4.0 mL purple top (EDTA whole blood) is a suboptimal specimen and results will be reported with this disclaimer: "Low volume sample, results may be unreliable."
Ordering

Performed

Daily

Methodology

Automated Cell Counter.

Reported

Routine requests are reported within 8 hours.

Synonyms

  • LAB301
  • PLT
Result Interpretation

Reference Interval

150 - 400 10^9/L

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

85049

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