VERISTRAT - For Laboratory Use ONLY

Collect

5.0 mL gold or red top tube with gel (SST)

Storage/Transport Temperature

Internal: Deliver to lab ASAP.

Remarks

Cancer Center staff should order test in CERNER (Lab computer system) and complete VERISTRAT Test requisition according to Biodesix recommendations and forward sample and VERISTRAT requisiton to the PCL Clinical Lab for processing.

Please call the PCL Clinical Lab @ 720-848-4401 and ask for Sendouts if you have any questions.

Performed

As needed.
Collection

Collect

5.0 mL gold or red top tube with gel (SST)

Storage/Transport Temperature

Internal: Deliver to lab ASAP.

Remarks

Cancer Center staff should order test in CERNER (Lab computer system) and complete VERISTRAT Test requisition according to Biodesix recommendations and forward sample and VERISTRAT requisiton to the PCL Clinical Lab for processing.

Please call the PCL Clinical Lab @ 720-848-4401 and ask for Sendouts if you have any questions.

Ordering

Performed

As needed.
Result Interpretation
Administrative

VIRAL CULTURE

LAB254
UHCVIRUS
 

Collect

Any sample appropriate to clinical syndrome. Submit in a sterile container or M6 transport media. Samples on swabs must be submitted in M6 transport media.

Blood, serum and plasma are not acceptable sample types.

Pediatric Collection

2 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; sample more than 72 hours old; sample not refrigerated/frozen at specified temperatures; swabs not submitted in viral transport medis; blood, serum or plasma sample types.

Storage/Transport Temperature

Internal: Deliver to lab immediately. Specimen must be kept cold and moist.
Offsite: Refrigerate or keep on ice. Transport same day. If delivery is not expected within 24 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Temperature-labile organisms. Each virus varies for stability.

Refrigerated: 24 hours; Frozen (-70 degrees C): Greater than 24 hours

Remarks

Requisition must state precise SOURCE of specimen, date of collection, age of patient, and relevant vaccinations. LAB3270 VIRAL CULTURE - CSF will be ordered for CSF specimens.

Performed by

PCL Clinical Laboratory - Virology

Performed

Daily

Methodology

Cell culture

Reported

18-28 days, depending on upper or lower respiratory source.

Synonyms

  • LAB254
  • UHCVIRUS

Performed by

PCL Clinical Laboratory - Virology

CPT Codes

87252. Additional charges are possible based on findings.

Collection

LAB254
UHCVIRUS
 

Collect

Any sample appropriate to clinical syndrome. Submit in a sterile container or M6 transport media. Samples on swabs must be submitted in M6 transport media.

Blood, serum and plasma are not acceptable sample types.

Pediatric Collection

2 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; sample more than 72 hours old; sample not refrigerated/frozen at specified temperatures; swabs not submitted in viral transport medis; blood, serum or plasma sample types.

Storage/Transport Temperature

Internal: Deliver to lab immediately. Specimen must be kept cold and moist.
Offsite: Refrigerate or keep on ice. Transport same day. If delivery is not expected within 24 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Temperature-labile organisms. Each virus varies for stability.

Refrigerated: 24 hours; Frozen (-70 degrees C): Greater than 24 hours

Remarks

Requisition must state precise SOURCE of specimen, date of collection, age of patient, and relevant vaccinations. LAB3270 VIRAL CULTURE - CSF will be ordered for CSF specimens.

Performed by

PCL Clinical Laboratory - Virology

Ordering

Performed

Daily

Methodology

Cell culture

Reported

18-28 days, depending on upper or lower respiratory source.

Synonyms

  • LAB254
  • UHCVIRUS
Result Interpretation

Performed by

PCL Clinical Laboratory - Virology

Administrative

CPT Codes

87252. Additional charges are possible based on findings.

VIRAL CULTURE - CSF

LAB3270
CVCSF

Collect

At least 0.5 mL CSF in a sterile container.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; sample not refrigerated/frozen at specified temperatures; specimens that are grossly contaminated, bloody, or non-CSF.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Refrigerate or keep on ice. Transport same day. If delivery is not expected within 24 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Temperature-labile organisms

Refrigerated: 24 hours; Frozen (-70 degrees C): Greater than 24 hours

Remarks

Preliminary report sent at 7 days; final at 14 days. LAB254 VIRAL CULTURE will be ordered for non-CSF specimens.

Performed by

PCL Clinical Laboratory - Virology

Performed

Daily

Methodology

Cell culture

Reported

14 days

Synonyms

  • LAB3270
  • CVCSF

Performed by

PCL Clinical Laboratory - Virology

CPT Codes

87252, 87253.
Additional charges are possible based on findings.

Collection

LAB3270
CVCSF

Collect

At least 0.5 mL CSF in a sterile container.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; sample not refrigerated/frozen at specified temperatures; specimens that are grossly contaminated, bloody, or non-CSF.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Refrigerate or keep on ice. Transport same day. If delivery is not expected within 24 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Temperature-labile organisms

Refrigerated: 24 hours; Frozen (-70 degrees C): Greater than 24 hours

Remarks

Preliminary report sent at 7 days; final at 14 days. LAB254 VIRAL CULTURE will be ordered for non-CSF specimens.

Performed by

PCL Clinical Laboratory - Virology

Ordering

Performed

Daily

Methodology

Cell culture

Reported

14 days

Synonyms

  • LAB3270
  • CVCSF
Result Interpretation

Performed by

PCL Clinical Laboratory - Virology

Administrative

CPT Codes

87252, 87253.
Additional charges are possible based on findings.

VIRAL EM FECES

LAB3335

Collect

Random stool. .

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: Min. volume = 1 g liquid stool Deliver to lab ASAP.

Stability (from collection to initiation)

Ambient or Refrigerated. [Frozen samples acceptable but not preferred.]

Remarks

Test is an electron microscopic scan of stool samples for viruses.

Performed by

The Children's Hospital - Aurora

Performed

Upon receipt

Methodology

Electron microscopy.

Reported

In 4 days

Synonyms

  • LAB3335

Performed by

The Children's Hospital - Aurora

CPT Codes

88348

Collection

LAB3335

Collect

Random stool. .

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: Min. volume = 1 g liquid stool Deliver to lab ASAP.

Stability (from collection to initiation)

Ambient or Refrigerated. [Frozen samples acceptable but not preferred.]

Remarks

Test is an electron microscopic scan of stool samples for viruses.

Performed by

The Children's Hospital - Aurora

Ordering

Performed

Upon receipt

Methodology

Electron microscopy.

Reported

In 4 days

Synonyms

  • LAB3335
Result Interpretation

Performed by

The Children's Hospital - Aurora

Administrative

CPT Codes

88348

VISCOSITY WHOLE BLOOD

LAB3192

Collect

Lavender (EDTA), pink (K2 EDTA), or green (sodium or lithium heparin).

Specimen Preparation

DO NOT FREEZE. Transport 3 mL whole blood. (Min: 0.6 mL)

Unacceptable Conditions

Frozen specimens. Clotted or hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 4 days; Frozen: Unacceptable

Performed

Mon-Fri

Methodology

Quantitative Viscometry

Reported

1-4 days

Synonyms

  • LAB3192
  • VISC WB

Ordering Recommendations

Evaluate hyperviscosity syndrome associated with plasma cell dyscrasia.

Reference Interval

0 up to 1 month: 3.6-6.5 cP
1 month and older: 3.6-6.0 cP

CPT Codes

85810
Collection

LAB3192

Collect

Lavender (EDTA), pink (K2 EDTA), or green (sodium or lithium heparin).

Specimen Preparation

DO NOT FREEZE. Transport 3 mL whole blood. (Min: 0.6 mL)

Unacceptable Conditions

Frozen specimens. Clotted or hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 4 days; Frozen: Unacceptable
Ordering

Performed

Mon-Fri

Methodology

Quantitative Viscometry

Reported

1-4 days

Synonyms

  • LAB3192
  • VISC WB

Ordering Recommendations

Evaluate hyperviscosity syndrome associated with plasma cell dyscrasia.
Result Interpretation

Reference Interval

0 up to 1 month: 3.6-6.5 cP
1 month and older: 3.6-6.0 cP
Administrative

CPT Codes

85810

VISCOSITY, SERUM

LAB765

Collect

Serum separator tube.

Specimen Preparation

Transfer 3 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Unacceptable Conditions

Clotted or hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 8 hours; Refrigerated: 4 days; Frozen: 1 month

Performed

Mon-Fri

Methodology

Quantitative Viscometry

Reported

1-4 days

Synonyms

  • Serum Viscosity
  • LAB765

Ordering Recommendations

Evaluate plasma hyperviscosity syndrome associated with disorders such as polycythemia, macroglobulinemia, multiple myeloma, and leukemia.

Reference Interval

1.10-1.80 cP

CPT Codes

85810
Collection

LAB765

Collect

Serum separator tube.

Specimen Preparation

Transfer 3 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Unacceptable Conditions

Clotted or hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 8 hours; Refrigerated: 4 days; Frozen: 1 month
Ordering

Performed

Mon-Fri

Methodology

Quantitative Viscometry

Reported

1-4 days

Synonyms

  • Serum Viscosity
  • LAB765

Ordering Recommendations

Evaluate plasma hyperviscosity syndrome associated with disorders such as polycythemia, macroglobulinemia, multiple myeloma, and leukemia.
Result Interpretation

Reference Interval

1.10-1.80 cP
Administrative

CPT Codes

85810

VITAMIN A [RETINOL A]

LAB580

Collect

Green (sodium or lithium heparin), plasma separator tube, or serum separator tube. Also acceptable: Lavender (EDTA) or pink (K2EDTA).

Patient Preparation

Patient should fast for 12 hours and abstain from alcohol for 24 hours prior to collection.

Specimen Preparation

Separate serum or plasma within 1 hour of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube immediately. (Min: 0.2 mL) Avoid hemolysis.

Unacceptable Conditions

Whole blood or body fluids other than serum or plasma.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 month; Frozen: 1 year

Notes

Serum retinol is typically maintained until hepatic stores are almost depleted. Values greater than 0.30 mg/L represent adequate liver stores, whereas values less than 0.10 mg/L indicate deficiency. Samples that come in contact with plastic tubing or have been exposed to excessive light may show low results.

Vitamin A toxicity occurs when retinol concentration exceeds the capacity of retinol binding protein (RBP). Individuals with compromised renal function can retain RBP and may, therefore, have moderate retinol elevations. Drugs which interfere with vitamin A analysis include probucol (Lorelco).

This assay does not measure other vitamin A metabolites such as retinaldehyde and retinoic acid.

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

1-4 days

Ordering Recommendations

Use for nutritional assessment of vitamin A (retinol and retinyl palmitate) in serum or plasma.

Reference Interval

Components
Reference Interval
Vitamin A (Retinol)
Age
Reference Interval
0-1 month
2 months-12 years
13-17 years
18 years and older
0.18-0.50 mg/L
0.20-0.50 mg/L
0.26-0.70 mg/L
0.30-1.20 mg/L

Vitamin A (Retinyl Palmitate)0-150 years: 0-0.10 mg/L

CPT Codes

84590
Collection

LAB580

Collect

Green (sodium or lithium heparin), plasma separator tube, or serum separator tube. Also acceptable: Lavender (EDTA) or pink (K2EDTA).

Patient Preparation

Patient should fast for 12 hours and abstain from alcohol for 24 hours prior to collection.

Specimen Preparation

Separate serum or plasma within 1 hour of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube immediately. (Min: 0.2 mL) Avoid hemolysis.

Unacceptable Conditions

Whole blood or body fluids other than serum or plasma.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 month; Frozen: 1 year

Notes

Serum retinol is typically maintained until hepatic stores are almost depleted. Values greater than 0.30 mg/L represent adequate liver stores, whereas values less than 0.10 mg/L indicate deficiency. Samples that come in contact with plastic tubing or have been exposed to excessive light may show low results.

Vitamin A toxicity occurs when retinol concentration exceeds the capacity of retinol binding protein (RBP). Individuals with compromised renal function can retain RBP and may, therefore, have moderate retinol elevations. Drugs which interfere with vitamin A analysis include probucol (Lorelco).

This assay does not measure other vitamin A metabolites such as retinaldehyde and retinoic acid.
Ordering

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

1-4 days

Ordering Recommendations

Use for nutritional assessment of vitamin A (retinol and retinyl palmitate) in serum or plasma.
Result Interpretation

Reference Interval

Components
Reference Interval
Vitamin A (Retinol)
Age
Reference Interval
0-1 month
2 months-12 years
13-17 years
18 years and older
0.18-0.50 mg/L
0.20-0.50 mg/L
0.26-0.70 mg/L
0.30-1.20 mg/L

Vitamin A (Retinyl Palmitate)0-150 years: 0-0.10 mg/L

Administrative

CPT Codes

84590

VITAMIN B1, WHOLE BLOOD

LAB125

Collect

Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation

Protect from light within one hour of collection and during storage and shipment. Transfer 3 mL whole blood to an ARUP Amber Transport Tube. (Min: 0.6 mL)

Unacceptable Conditions

Any specimen other than whole blood. Glass tubes. Clotted or non-frozen specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 6 months

Notes

Whole blood is the preferred specimen for thiamine assessment.  Approximately 80 percent of thiamine present in whole blood is found in red blood cells. 

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

2-4 days

Synonyms

  • Thiamine
  • TPP
  • VITB1
  • Thiamin Pyrophosphate
  • B1 Vitamin
  • Thiamine Diphosphate
  • TDP
  • LAB125

Ordering Recommendations

Use for nutritional assessment of vitamin B1 (thiamine).

Reference Interval

70-180 nmol/L

Interpretive Data

This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.

CPT Codes

84425
Collection

LAB125

Collect

Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation

Protect from light within one hour of collection and during storage and shipment. Transfer 3 mL whole blood to an ARUP Amber Transport Tube. (Min: 0.6 mL)

Unacceptable Conditions

Any specimen other than whole blood. Glass tubes. Clotted or non-frozen specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 6 months

Notes

Whole blood is the preferred specimen for thiamine assessment.  Approximately 80 percent of thiamine present in whole blood is found in red blood cells. 
Ordering

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

2-4 days

Synonyms

  • Thiamine
  • TPP
  • VITB1
  • Thiamin Pyrophosphate
  • B1 Vitamin
  • Thiamine Diphosphate
  • TDP
  • LAB125

Ordering Recommendations

Use for nutritional assessment of vitamin B1 (thiamine).
Result Interpretation

Reference Interval

70-180 nmol/L

Interpretive Data

This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.

Administrative

CPT Codes

84425

VITAMIN B12

LAB67

VITB12

Collect

One 4.5 mL green top with gel (PST), (lithium heparin plasma).

Pediatric Collection

At least 0.5 mL whole blood in green top microtainer with gel, (lithium heparin plasma) or other heparin tube.

Unacceptable Conditions

Grossly hemolyzed samples; EDTA anticoagulant; sample not properly identified; insufficient sample volume; improperly stored.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection.  If delivered within 4 hours of collection, transport to lab at 2-8 degrees C.  If delivered more than 4 hours after collection, place at least 1.0 mL plasma in an aliquot container and transport to the lab frozen.

Stability (from collection to initiation)

Before centrifugation: 1 hour.

After centrifugation: Ambient: 2 hours; Refrigerated: 4 hours.

Plasma aliquot: Ambient, 8 hours; Refrigerated, 24 hours; Frozen (-20 degrees C), 1 month.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Chemiluminescent immunoassay.

Reported

Daily

Synonyms

  • LAB67
  • VITB12

Reference Interval

180 - 914 pg/mL

Interpretive Data

Indeterminate Range: 145-180 pg/mL

Deficient Range:  <145 pg/mL

Performed by

PCL Clinical Laboratory - Core

CPT Codes

82607

Collection

LAB67

VITB12

Collect

One 4.5 mL green top with gel (PST), (lithium heparin plasma).

Pediatric Collection

At least 0.5 mL whole blood in green top microtainer with gel, (lithium heparin plasma) or other heparin tube.

Unacceptable Conditions

Grossly hemolyzed samples; EDTA anticoagulant; sample not properly identified; insufficient sample volume; improperly stored.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge within 30 minutes of collection.  If delivered within 4 hours of collection, transport to lab at 2-8 degrees C.  If delivered more than 4 hours after collection, place at least 1.0 mL plasma in an aliquot container and transport to the lab frozen.

Stability (from collection to initiation)

Before centrifugation: 1 hour.

After centrifugation: Ambient: 2 hours; Refrigerated: 4 hours.

Plasma aliquot: Ambient, 8 hours; Refrigerated, 24 hours; Frozen (-20 degrees C), 1 month.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Chemiluminescent immunoassay.

Reported

Daily

Synonyms

  • LAB67
  • VITB12
Result Interpretation

Reference Interval

180 - 914 pg/mL

Interpretive Data

Indeterminate Range: 145-180 pg/mL

Deficient Range:  <145 pg/mL

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

82607

VITAMIN B6, TOTAL

LAB120

Collect

Green (sodium or lithium heparin) or plasma separator tube. Also acceptable: Plain red.

Patient Preparation

Collect specimen after an overnight fast.

Specimen Preparation

Protect from light during collection, storage, and shipment. Separate plasma or serum from cells within 1 hour of collection. Transfer 1 mL plasma or serum to an ARUP Amber Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Any specimens other than heparinized plasma or serum. Serum separator tubes or EDTA preserved tubes. Hemolyzed specimens. Specimens not protected from light.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 3 days; Frozen: 2 months

Notes

This test measures pyridoxal 5-phosphate, the biologically active form of vitamin B6.

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

1-4 days

Synonyms

  • B6 Vitamin, Plasma
  • Pyridoxine
  • Pyridoxal 5-Phos
  • Pyridoxal
  • LAB120

Ordering Recommendations

Use for nutritional assessment of vitamin B6.

Reference Interval

20-125 nmol/L

Interpretive Data

Pyridoxal 5'-phosphate measured in a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.

CPT Codes

84207
Collection

LAB120

Collect

Green (sodium or lithium heparin) or plasma separator tube. Also acceptable: Plain red.

Patient Preparation

Collect specimen after an overnight fast.

Specimen Preparation

Protect from light during collection, storage, and shipment. Separate plasma or serum from cells within 1 hour of collection. Transfer 1 mL plasma or serum to an ARUP Amber Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Any specimens other than heparinized plasma or serum. Serum separator tubes or EDTA preserved tubes. Hemolyzed specimens. Specimens not protected from light.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 3 days; Frozen: 2 months

Notes

This test measures pyridoxal 5-phosphate, the biologically active form of vitamin B6.
Ordering

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

1-4 days

Synonyms

  • B6 Vitamin, Plasma
  • Pyridoxine
  • Pyridoxal 5-Phos
  • Pyridoxal
  • LAB120

Ordering Recommendations

Use for nutritional assessment of vitamin B6.
Result Interpretation

Reference Interval

20-125 nmol/L

Interpretive Data

Pyridoxal 5'-phosphate measured in a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.

Administrative

CPT Codes

84207

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