Tests Catalogs

ADALIMUMAB LEVEL/NEUTRALIZING ANTIBODIES

LAB5451

Collect

6.0 mL Gold Top Tube

Specimen Preparation

Additiona Information needed for sample submission:

Reason for order and any Prior/Current dosing information.

 

Unacceptable Conditions

Frozen samples.

Storage/Transport Temperature

.Refrigerated

Stability (from collection to initiation)

Ambient: Serum stable 7 days

Refrigerated: Serum stable 9 days

Performed by

Prometheus Therapeutics & Diagnostics

Ordering Recommendations

Testing should only be ordered on patients who are receiveing Adalimumab (Humira).

Performed by

Prometheus Therapeutics & Diagnostics

CPT Codes

TBD

Collection

LAB5451

Collect

6.0 mL Gold Top Tube

Specimen Preparation

Additiona Information needed for sample submission:

Reason for order and any Prior/Current dosing information.

 

Unacceptable Conditions

Frozen samples.

Storage/Transport Temperature

.Refrigerated

Stability (from collection to initiation)

Ambient: Serum stable 7 days

Refrigerated: Serum stable 9 days

Performed by

Prometheus Therapeutics & Diagnostics

Ordering

Ordering Recommendations

Testing should only be ordered on patients who are receiveing Adalimumab (Humira).

Result Interpretation

Performed by

Prometheus Therapeutics & Diagnostics

Administrative

CPT Codes

TBD

ADAMTS13 ACTIVITY

LAB3134

Collect

Lt. blue (sodium citrate). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation

Transfer 1 mL platelet-poor plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Serum, EDTA plasma, clotted or hemolyzed specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: Unacceptable; Frozen: 2 weeks

Performed

Mon-Sun

Methodology

Chromogenic Assay

Reported

1-3 days

Synonyms

  • LAB3134
  • ADAMTS-13

Ordering Recommendations

Assist in diagnosing congenital or inherited thrombotic thrombocytopenic purpura (TTP).

Reference Interval

Effective November 17, 2014

Greater than 60 percent

Interpretive Data

ADAMTS13 levels of less than 5 percent may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP).

A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may raise the observed ADAMTS13 activity.

CPT Codes

85397
Collection

LAB3134

Collect

Lt. blue (sodium citrate). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation

Transfer 1 mL platelet-poor plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Serum, EDTA plasma, clotted or hemolyzed specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: Unacceptable; Frozen: 2 weeks
Ordering

Performed

Mon-Sun

Methodology

Chromogenic Assay

Reported

1-3 days

Synonyms

  • LAB3134
  • ADAMTS-13

Ordering Recommendations

Assist in diagnosing congenital or inherited thrombotic thrombocytopenic purpura (TTP).
Result Interpretation

Reference Interval

Effective November 17, 2014

Greater than 60 percent

Interpretive Data

ADAMTS13 levels of less than 5 percent may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP).

A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may raise the observed ADAMTS13 activity.

Administrative

CPT Codes

85397

ADENOSINE DEAMINASE - PLEURAL FLUID

LAB8424

Collect

Body fluids must be spun, aliquoted and frozen within 24 hours.

Specimen Preparation

Centrifuge and aliquot 0.3 mL fluid. Transfer supernatant to plastic vial freeze ASAP and ship frozen.

Unacceptable Conditions

Hemolyzed samples/samples contaminated with blood.

Storage/Transport Temperature

Refrigerated: <24 hours

Store frozen.

Performed by

ARUP Laboratory

Performed

3 – 5 times per week

Methodology

Ultraviolet Spectrophotometry

Reported

In 2 days.

Reference Interval

REFERENCE VALUES:         ¦
¦Pleural Exudate:       

¦Total protein >3.0 g/dL:  1.6-9.2 U/L                                         ¦
¦                                                                              ¦
¦Pleural Transudate:                                                           ¦
¦Total protein <3.0 g/dL:  0-6.7 U/L                                           ¦
¦                                                                              ¦
¦Peritoneal Exudate:                                                           ¦
¦Total protein >2.0 g/dL:  0-7.6 U/L                                           ¦
¦                                                                              ¦
¦Peritoneal Transudate:                                                        ¦
¦Total protein <2.0 g/dL:  0-7.6 U/L                                           ¦
¦                                                                              ¦
¦Ranges for other fluids not established.

Performed by

ARUP Laboratory

Collection

LAB8424

Collect

Body fluids must be spun, aliquoted and frozen within 24 hours.

Specimen Preparation

Centrifuge and aliquot 0.3 mL fluid. Transfer supernatant to plastic vial freeze ASAP and ship frozen.

Unacceptable Conditions

Hemolyzed samples/samples contaminated with blood.

Storage/Transport Temperature

Refrigerated: <24 hours

Store frozen.

Performed by

ARUP Laboratory

Ordering

Performed

3 – 5 times per week

Methodology

Ultraviolet Spectrophotometry

Reported

In 2 days.

Result Interpretation

Reference Interval

REFERENCE VALUES:         ¦
¦Pleural Exudate:       

¦Total protein >3.0 g/dL:  1.6-9.2 U/L                                         ¦
¦                                                                              ¦
¦Pleural Transudate:                                                           ¦
¦Total protein <3.0 g/dL:  0-6.7 U/L                                           ¦
¦                                                                              ¦
¦Peritoneal Exudate:                                                           ¦
¦Total protein >2.0 g/dL:  0-7.6 U/L                                           ¦
¦                                                                              ¦
¦Peritoneal Transudate:                                                        ¦
¦Total protein <2.0 g/dL:  0-7.6 U/L                                           ¦
¦                                                                              ¦
¦Ranges for other fluids not established.

Performed by

ARUP Laboratory

Administrative

ADENOSINE DEAMINASE PERICARDIAL FLUID-ARUP

LAB8426

Collect

Pericardial Fluid

Specimen Preparation

Centrifuge specimen at room temperature. Transfer 0.3 mL fluid to an ARUP Standard Transport Tube and freeze. (Min: 0.1 mL)

Unacceptable Conditions

Whole blood or Bronchoalveolar Lavage (BAL) specimens. Turbid specimens.

Storage/Transport Temperature

Frozen. Specimen must remain frozen until received in lab.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 7 days; Frozen: 30 days

Remarks

Indicate source on requisition.

Performed by

ARUP

Performed

Sun, Tue, Thu

Methodology

Quantitative Spectrophotometry

Reported

1-4 days

Reference Interval

0-11.3 U/L

Performed by

ARUP

CPT Codes

84311
Collection

LAB8426

Collect

Pericardial Fluid

Specimen Preparation

Centrifuge specimen at room temperature. Transfer 0.3 mL fluid to an ARUP Standard Transport Tube and freeze. (Min: 0.1 mL)

Unacceptable Conditions

Whole blood or Bronchoalveolar Lavage (BAL) specimens. Turbid specimens.

Storage/Transport Temperature

Frozen. Specimen must remain frozen until received in lab.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 7 days; Frozen: 30 days

Remarks

Indicate source on requisition.

Performed by

ARUP

Ordering

Performed

Sun, Tue, Thu

Methodology

Quantitative Spectrophotometry

Reported

1-4 days
Result Interpretation

Reference Interval

0-11.3 U/L

Performed by

ARUP

Administrative

CPT Codes

84311

ADENOSINE DEAMINASE PERITONEAL FLUID-ARUP

LAB8425

Collect

Peritoneal Fluid.

Patient Preparation

Collect specimens in leak proof container.

Specimen Preparation

Centrifuge specimen at room temperature. Transfer 0.3 mL fluid to an ARUP Standard Transport Tube and freeze. (Min: 0.1 mL)

Unacceptable Conditions

Whole blood. Bronchoalveolar Lavage (BAL) specimens. Turbid specimens.

Storage/Transport Temperature

Frozen. Specimen must remain frozen until received in lab.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 1 week; Frozen: 1 month

Remarks

Indicate source on requisition.

Performed by

ARUP

Performed

Sun, Tue, Thu

Methodology

Quantitative Spectrophotometry

Reported

1-4 days

Reference Interval

0.0-7.3 U/L

Performed by

ARUP

CPT Codes

84311
Collection

LAB8425

Collect

Peritoneal Fluid.

Patient Preparation

Collect specimens in leak proof container.

Specimen Preparation

Centrifuge specimen at room temperature. Transfer 0.3 mL fluid to an ARUP Standard Transport Tube and freeze. (Min: 0.1 mL)

Unacceptable Conditions

Whole blood. Bronchoalveolar Lavage (BAL) specimens. Turbid specimens.

Storage/Transport Temperature

Frozen. Specimen must remain frozen until received in lab.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 1 week; Frozen: 1 month

Remarks

Indicate source on requisition.

Performed by

ARUP

Ordering

Performed

Sun, Tue, Thu

Methodology

Quantitative Spectrophotometry

Reported

1-4 days
Result Interpretation

Reference Interval

0.0-7.3 U/L

Performed by

ARUP

Administrative

CPT Codes

84311

ADENOVIRUS BY PCR

LAB3239

Collect

All sample types, except blood.  Submit in a sterile container.

Specimen source is required.

If submitting a Pink top tube (EDTA), refer to test code ADENOPLS.

Pediatric Collection

0.5mL

Unacceptable Conditions

Sample not properly identified; insufficient sample volume; contaminated specimen; clotted sample; gross hemolysis; non sterile or leaking containers; and samples containing fixatives.

For plasma, refer to test code ADENOPLS.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if delivery is less than 72 hours. If delivery is not expected within 72 hours, freeze at -70 degrees C and ship frozen.

Stability (from collection to initiation)

Ambient:  12 hours; Refrigerated: 72 hours;  Frozen (-70 degrees C): 6 months

Remarks

Quantitative results will be given for all urine samples. Qualitative results will be given for tissues, stools, swabs, or diluted samples.

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Performed

Mon - Fri

Methodology

DNA amplification by polymerase chain reaction (PCR) using an Adenovirus-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Interpretive Data

Qualitative testing:

TND = Target Not Detected (negative)

Positive = Virus Detected

Quantitative testing:

TND = Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001 - 1,000,000 copies/mL = Quantitative range

>1,000,000 copies/mL = Greater than maximum quantitative range

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

CPT Codes

87799 Quantitative; 87798 Qualitative

Collection

LAB3239

Collect

All sample types, except blood.  Submit in a sterile container.

Specimen source is required.

If submitting a Pink top tube (EDTA), refer to test code ADENOPLS.

Pediatric Collection

0.5mL

Unacceptable Conditions

Sample not properly identified; insufficient sample volume; contaminated specimen; clotted sample; gross hemolysis; non sterile or leaking containers; and samples containing fixatives.

For plasma, refer to test code ADENOPLS.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if delivery is less than 72 hours. If delivery is not expected within 72 hours, freeze at -70 degrees C and ship frozen.

Stability (from collection to initiation)

Ambient:  12 hours; Refrigerated: 72 hours;  Frozen (-70 degrees C): 6 months

Remarks

Quantitative results will be given for all urine samples. Qualitative results will be given for tissues, stools, swabs, or diluted samples.

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Ordering

Performed

Mon - Fri

Methodology

DNA amplification by polymerase chain reaction (PCR) using an Adenovirus-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Result Interpretation

Interpretive Data

Qualitative testing:

TND = Target Not Detected (negative)

Positive = Virus Detected

Quantitative testing:

TND = Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001 - 1,000,000 copies/mL = Quantitative range

>1,000,000 copies/mL = Greater than maximum quantitative range

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Administrative

CPT Codes

87799 Quantitative; 87798 Qualitative

Adenovirus by PCR, plasma

LAB3238

Collect

One 6 mL Pink top tube (EDTA). Also Acceptable: One 4 mL Purple top tube (EDTA).

Specimen source is required to be included on requisition.

Pediatric Collection

At least 0.5 mL of whole blood collected in a 1.0mL pink top microtainer (EDTA) or other blood tube with EDTA anticoagulant.

Unacceptable Conditions

Sample not properly identified; insufficient sample volume; contaminated specimen; clotted sample; tubes not filled to 80-100% capacity; improper storage conditions; gross hemolysis; samples stored in fixatives.

Storage/Transport Temperature

Internal: Deliver to lab immediately.

Offsite: Send at 2-8 degrees C if tested within 72 hours. If delivery is not expected within 72 hours, centrifuge, transfer supernatant to an aliquot tube, and freeze at -70 degrees C. Ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours; Refrigerated: 3 days; Frozen (-70 degres C): 6 months.

Remarks

Quantitative results will be reported.

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Performed

Mon-Fri.

Methodology

DNA amplification by polymerase chain reaction (PCR) using a Adenovirus-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Interpretive Data

TND - Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001-1,000,000 copies/mL = Quantitative ranges

>1,000,000 copies/mL = Greater than maximum quantitative range.

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

CPT Codes

87799

Collection

LAB3238

Collect

One 6 mL Pink top tube (EDTA). Also Acceptable: One 4 mL Purple top tube (EDTA).

Specimen source is required to be included on requisition.

Pediatric Collection

At least 0.5 mL of whole blood collected in a 1.0mL pink top microtainer (EDTA) or other blood tube with EDTA anticoagulant.

Unacceptable Conditions

Sample not properly identified; insufficient sample volume; contaminated specimen; clotted sample; tubes not filled to 80-100% capacity; improper storage conditions; gross hemolysis; samples stored in fixatives.

Storage/Transport Temperature

Internal: Deliver to lab immediately.

Offsite: Send at 2-8 degrees C if tested within 72 hours. If delivery is not expected within 72 hours, centrifuge, transfer supernatant to an aliquot tube, and freeze at -70 degrees C. Ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours; Refrigerated: 3 days; Frozen (-70 degres C): 6 months.

Remarks

Quantitative results will be reported.

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Ordering

Performed

Mon-Fri.

Methodology

DNA amplification by polymerase chain reaction (PCR) using a Adenovirus-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Result Interpretation

Interpretive Data

TND - Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001-1,000,000 copies/mL = Quantitative ranges

>1,000,000 copies/mL = Greater than maximum quantitative range.

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Administrative

CPT Codes

87799

ADRENOCORTICOTROPIC HORMONE

LAB511

Collect

Lavender (EDTA) or pink (K2EDTA). Collection tube must be siliconized glass or plastic.

Specimen Preparation

Separate plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.5 mL)

Unacceptable Conditions

Serum, tissue or urine. Heparinized plasma. Grossly hemolyzed or lipemic specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: 24 hours; Refrigerated: 72 hours; Frozen 3 months.

Performed

Sun-Sat

Methodology

Quantitative Chemiluminescent Immunoassay

Reported

1-2 days

Synonyms

  • ACTH
  • LAB511

Ordering Recommendations

Aids in the diagnosis of adrenal insufficiency and determining the presence of anterior pituitary tumors.

Reference Interval

Male and female - 1 week-9 years: 5-46 pg/mL
Male and female - 10-18 years: 6-55 pg/mL
Female - 19 years and older: 6-58 pg/mL
Male - 19 years and older: 7-69 pg/mL

Interpretive Data

Some types of synthetic ACTH are not detected by this assay.

CPT Codes

82024
Collection

LAB511

Collect

Lavender (EDTA) or pink (K2EDTA). Collection tube must be siliconized glass or plastic.

Specimen Preparation

Separate plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.5 mL)

Unacceptable Conditions

Serum, tissue or urine. Heparinized plasma. Grossly hemolyzed or lipemic specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: 24 hours; Refrigerated: 72 hours; Frozen 3 months.
Ordering

Performed

Sun-Sat

Methodology

Quantitative Chemiluminescent Immunoassay

Reported

1-2 days

Synonyms

  • ACTH
  • LAB511

Ordering Recommendations

Aids in the diagnosis of adrenal insufficiency and determining the presence of anterior pituitary tumors.
Result Interpretation

Reference Interval

Male and female - 1 week-9 years: 5-46 pg/mL
Male and female - 10-18 years: 6-55 pg/mL
Female - 19 years and older: 6-58 pg/mL
Male - 19 years and older: 7-69 pg/mL

Interpretive Data

Some types of synthetic ACTH are not detected by this assay.

Administrative

CPT Codes

82024

AFB CULTURE

LAB3452

Collect

In a sterile container:

  • 5-10 mL (minimum volume 2 mL) respiratory specimens. Submit three early moring specimens on three consecutive days.  Early morning specimens are preferred.
  • To expedite the patient's discharge, submit 3 sputa, one in the early morning and the remaining two at least 4 hours apart in one 24 hour period.  All three specimens will be processed the following day.
  • May be induced if necessary.
  • 5-10 mL (minimum volume 2 mL) body fluids, tissues, exudates, cutaneous, gastric, etc.
  • 50 mL urine
  • 10 mL CSF (minimum volume 6 mL)
  • Blood cultures for AFB: Two 7 mL green top tubes.
Stools for AFB must be approved by the microbiology director.

Swabs are unacceptable for culture.

Unacceptable Conditions

Specimens submitted on swabs; Respiratory specimens with volumes under 5 mL or obvious majority of saliva; Gastric specimens that have not been neutralized and have a delayed transport to the lab; specimens not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Date and refrigerate specimens. Specimens must be delivered to the laboratory as soon as possible (no more than 2 days) for optimal recovery of AFB.

Stability (from collection to initiation)

Refrigerated: 48-72 hours.

Remarks

Test includes Mycobacterial (AFB) culture and stain. Physicians will be notified of all positive cultures and/or stains. Negative cultures will be reported after 8 weeks.

Sensitivity testing will be performed on M. tuberculosis by an outside laboratory. Sensitivity testing on MOTB must be approved by Infectious Disease.

Respiratory specimens: Since 5-10 mL sputum (not saliva) is required, specimens may be collected over 1-2 hours. Refrigerate if immediate delivery to lab is not possible.

Urine specimens: No AFB stain is performed due to the presence of saprophytic mycobacteria in genital secretions of normal patients.

CSF specimens: No AFB stain is performed due to extremely low sensitivity of the test.

Performed by

PCL Clinical Laboratory - Microbiology

Performed

Daily

Methodology

Mycobacterial culture and stain.

Reported

Up to 8 Weeks

Synonyms

  • LAB3452

Performed by

PCL Clinical Laboratory - Microbiology

CPT Codes

87116

Collection

LAB3452

Collect

In a sterile container:

  • 5-10 mL (minimum volume 2 mL) respiratory specimens. Submit three early moring specimens on three consecutive days.  Early morning specimens are preferred.
  • To expedite the patient's discharge, submit 3 sputa, one in the early morning and the remaining two at least 4 hours apart in one 24 hour period.  All three specimens will be processed the following day.
  • May be induced if necessary.
  • 5-10 mL (minimum volume 2 mL) body fluids, tissues, exudates, cutaneous, gastric, etc.
  • 50 mL urine
  • 10 mL CSF (minimum volume 6 mL)
  • Blood cultures for AFB: Two 7 mL green top tubes.
Stools for AFB must be approved by the microbiology director.

Swabs are unacceptable for culture.

Unacceptable Conditions

Specimens submitted on swabs; Respiratory specimens with volumes under 5 mL or obvious majority of saliva; Gastric specimens that have not been neutralized and have a delayed transport to the lab; specimens not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Date and refrigerate specimens. Specimens must be delivered to the laboratory as soon as possible (no more than 2 days) for optimal recovery of AFB.

Stability (from collection to initiation)

Refrigerated: 48-72 hours.

Remarks

Test includes Mycobacterial (AFB) culture and stain. Physicians will be notified of all positive cultures and/or stains. Negative cultures will be reported after 8 weeks.

Sensitivity testing will be performed on M. tuberculosis by an outside laboratory. Sensitivity testing on MOTB must be approved by Infectious Disease.

Respiratory specimens: Since 5-10 mL sputum (not saliva) is required, specimens may be collected over 1-2 hours. Refrigerate if immediate delivery to lab is not possible.

Urine specimens: No AFB stain is performed due to the presence of saprophytic mycobacteria in genital secretions of normal patients.

CSF specimens: No AFB stain is performed due to extremely low sensitivity of the test.

Performed by

PCL Clinical Laboratory - Microbiology

Ordering

Performed

Daily

Methodology

Mycobacterial culture and stain.

Reported

Up to 8 Weeks

Synonyms

  • LAB3452
Result Interpretation

Performed by

PCL Clinical Laboratory - Microbiology

Administrative

CPT Codes

87116

AFB STAIN

LAB3452

Collect

In a sterile container:

  • 5-10 mL (minimum volume 2 mL) respiratory specimens. Submit three early moring specimens on three consecutive days.  Early morning specimens are preferred.
  • To expedite the patient's discharge, submit 3 sputa, one in the early morning and the remaining two at least 4 hours apart in one 24 hour period.  All three specimens will be processed the following day.
  • May be induced if necessary.
  • 5-10 mL (minimum volume 2 mL) body fluids, tissues, exudates, cutaneous, gastric, etc.
  • 50 mL urine
  • 10 mL CSF (minimum volume 6 mL)
  • Blood cultures for AFB: Two 7 mL green top tubes.
Stools for AFB must be approved by the microbiology director.

Swabs are unacceptable for culture.

Unacceptable Conditions

Specimens submitted on swabs; Respiratory specimens with volumes under 5 mL or obvious majority of saliva; Gastric specimens that have not been neutralized and have a delayed transport to the lab; specimens not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Date and refrigerate specimens. Specimens must be delivered to the laboratory as soon as possible (no more than 2 days) for optimal recovery of AFB.

Stability (from collection to initiation)

Refrigerated: 48-72 hours.

Remarks

Test includes Mycobacterial (AFB) culture and stain. Physicians will be notified of all positive cultures and/or stains. Negative cultures will be reported after 8 weeks.

Sensitivity testing will be performed on M. tuberculosis by an outside laboratory. Sensitivity testing on MOTB must be approved by Infectious Disease.

Respiratory specimens: Since 5-10 mL sputum (not saliva) is required, specimens may be collected over 1-2 hours. Refrigerate if immediate delivery to lab is not possible.

Urine specimens: No AFB stain is performed due to the presence of saprophytic mycobacteria in genital secretions of normal patients.

CSF specimens: No AFB stain is performed due to extremely low sensitivity of the test.

Performed by

PCL Clinical Laboratory - Microbiology

Performed

Daily

Methodology

Mycobacterial culture and stain.

Reported

Up to 8 Weeks

Synonyms

  • LAB3452

Performed by

PCL Clinical Laboratory - Microbiology

CPT Codes

87116

Collection

LAB3452

Collect

In a sterile container:

  • 5-10 mL (minimum volume 2 mL) respiratory specimens. Submit three early moring specimens on three consecutive days.  Early morning specimens are preferred.
  • To expedite the patient's discharge, submit 3 sputa, one in the early morning and the remaining two at least 4 hours apart in one 24 hour period.  All three specimens will be processed the following day.
  • May be induced if necessary.
  • 5-10 mL (minimum volume 2 mL) body fluids, tissues, exudates, cutaneous, gastric, etc.
  • 50 mL urine
  • 10 mL CSF (minimum volume 6 mL)
  • Blood cultures for AFB: Two 7 mL green top tubes.
Stools for AFB must be approved by the microbiology director.

Swabs are unacceptable for culture.

Unacceptable Conditions

Specimens submitted on swabs; Respiratory specimens with volumes under 5 mL or obvious majority of saliva; Gastric specimens that have not been neutralized and have a delayed transport to the lab; specimens not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Date and refrigerate specimens. Specimens must be delivered to the laboratory as soon as possible (no more than 2 days) for optimal recovery of AFB.

Stability (from collection to initiation)

Refrigerated: 48-72 hours.

Remarks

Test includes Mycobacterial (AFB) culture and stain. Physicians will be notified of all positive cultures and/or stains. Negative cultures will be reported after 8 weeks.

Sensitivity testing will be performed on M. tuberculosis by an outside laboratory. Sensitivity testing on MOTB must be approved by Infectious Disease.

Respiratory specimens: Since 5-10 mL sputum (not saliva) is required, specimens may be collected over 1-2 hours. Refrigerate if immediate delivery to lab is not possible.

Urine specimens: No AFB stain is performed due to the presence of saprophytic mycobacteria in genital secretions of normal patients.

CSF specimens: No AFB stain is performed due to extremely low sensitivity of the test.

Performed by

PCL Clinical Laboratory - Microbiology

Ordering

Performed

Daily

Methodology

Mycobacterial culture and stain.

Reported

Up to 8 Weeks

Synonyms

  • LAB3452
Result Interpretation

Performed by

PCL Clinical Laboratory - Microbiology

Administrative

CPT Codes

87116

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