Tests Catalogs

One 2.7 mL blue top (sodium citrate 3.2%).

Also acceptable: One 1.8 mL blue top tube (3.2% sodium citrate).

All blue tops collected for coagulation assays which do not have a blood culture collected first must have a discard tube collected prior to filling.Discard tube should be another blue top in which greater than 1 mL of blood is collected.

0.5 mL of platelet poor plasma.

Clotted sample; tube fill volume less than 90% or greater than 110%; whole blood specimen more than 1 hour since collection; platelet-poor plasma submitted more than 2 hours since collection; sample not properly identified; incorrect container; insufficient sample volume.

Internal: Deliver to lab immediately.
Offsite: Separate plasma from cells as soon as possible. Place plasma in aliquot container and transport to lab frozen.

Whole blood: Ambient, 1 hour

Platelet poor plasma aliquot: Ambient, 2 hours; Refrigerated, 2 hours; Frozen (-20 degrees C), 2 weeks;  Frozen (-70 degrees C), 6 months.

Therapeutic range: 0.3 - 0.7 units/mL

PCL Clinical Laboratory - Coagulation

Fresh tissue (preferred): Less than 1 cubic cm.

Body fluids.

Formalin fixed paraffin embedded tissue also acceptable.

(Formalin dramatically reduces the sensitivity of the assays due to reduced template yield and quality.)

List source of specimen.

Indicate type of Universal PCR testing desired (all or some combination of the following):

AFB

Bacterial

Fungal

Ambient:  Paraffin-embedded blocks of tissue.

Frozen:  Fresh tissue, body fluids.

University of Washington - Molecular Microbiology Laboratory in Seattle, WA.

1 mL fluid in sterile container

0.2 mL fluid

Sample not properly identified; incorrect container; insufficient sample volume.

Internal: Deliver to lab immediately
Offsite: Transport to lab at ambient temperature.

Ambient: 24 hours; Refrigerated: 5 days; Frozen (-20 degrees C): 3 months.

Identify fluid source on request form.

PCL Clinical Laboratory - Core

5 mL random urine in plastic container, no preservative.

0.2 mL urine

Sample not properly identified; incorrect container; insufficient sample volume.

Internal: Deliver to lab immediately
Offsite: Transport to lab at ambient temperature.

Ambient: 2 days; Refrigerated: 7 days; Frozen (-20 degrees C): 1 month.

PCL Clinical Laboratory - Core

Timed urine collection in plastic container.
Mandatory collection information: Start date and time and End date and time of collection.
 

Timed urine collection in plastic container. Minimum volume 0.2 mL urine.
Mandatory collection information: Start date and time and End date and time of collection.
 

Sample not properly identified; incorrect container; insufficient sample volume.

Internal: Deliver to lab immediately
Offsite: Transport to lab at ambient temperature.

Ambient: 2 days; Refrigerated: 7 days; Frozen (-20 degrees C): 1 month.

Requisition must state date and collection period. Refrigerate during collection.

PCL Clinical Laboratory - Core

Timed urine collection in plastic container.
Mandatory collection information: Start date and time and End date and time of collection.
 

Timed urine collection in plastic container. Minimum volume 0.2 mL urine.
Mandatory collection information: Start date and time and End date and time of collection.
 

Specimen not properly identified; incorrect container; insufficient sample volume.

Internal: Deliver to lab immediately.

Offsite: Transport to laboratory at ambient temperature.

Ambient: 2 days; Refrigerated: 7 days; Frozen (-20 degrees C): 1 month.

Requisition must state date and collection period (start time and end time). indicate total volume when submitting an aliquot. Refrigerate during collection.

PCL Clinical Laboratory - Core

5 mL random urine in plastic container, no preservative.

0.2 mL urine

Sample not properly identified; incorrect container; insufficient sample volume.

Internal: Deliver to lab immediately
Offsite: Transport to lab at ambient temperature.

Ambient: 3 days; Refrigerated: 7 days; Frozen (-20 degrees C): 6 months.

PCL Clinical Laboratory - Core

One 4.5 mL Green top with gel (PST), plasma (lithium heparin).

At least 0.5 mL whole blood collected in a microtainer, green top with gel, lithium heparin plasma.

Potassium oxalate/sodium fluoride anticoagulant (gray top); sample not maintained at correct temperature; sample not properly identified; incorrect container; insufficient sample volume.

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection. Transport to laboratory at 2-8 degrees C.

Before centrifugation: Ambient, 1 hour.

After centrifugation: Ambient, 8 hours; Refrigerated, 48 hours; Frozen, Unacceptable.

Plasma aliquot: Ambient: 3 days; Refrigerated: 7 days; Frozen (-20 degrees C): 6 months.

Patients on Rasburicase require special collection requirements:

1. Order Uric Acid as single test.

2. Collect Uric Acid in a pre-chilled green top tube with gel.

3. Transport on ice immediately.

4. Stable 4 hours on ice.

5. This Rasburicase (Elitek) protocol must be followed for 4 days after last dose.

PCL Clinical Laboratory - Core