Tests Catalogs

MISCELLANEOUS CULTURE

LAB3274

Collect

In a sterile container: pus from aspiration, bone marrow aspirate, corneal scrapings, wound exudate, drainage fluids, small pieces of tissue or biopsy, venous or arterial catheter tip, bile, gall bladder fluid, prostate tissue or fluid, placenta tissue, or genital swab; or

Copan eSwab in transport media. Swabs are not acceptable for tissue culture. Fluid, pus, or drainage is ALWAYS preferable to a swab.

For corneal and conjunctiva cultures, media is available in the laboratory if bedside inoculation is desired.

Pediatric Collection

In a sterile container, 0.5-2 mL fluid or 0.1 g viable tissue for quantitative burn cultures; or Copan eSwab in transport media.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; delayed transport to the laboratory, tissue in fixative, urinary catheters, grossly contaminated specimen. Swabs are not acceptable for tissue culture.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport ASAP at ambient temperature.

Stability (from collection to initiation)

Ambient (not in transport media): 1 hour. Ambient (in transport media): greater than 1 hour.

Remarks

Routine aerobic bacterial culture only. Miscellaneous cultures include abscess, aspirate, catheter tips, deep wounds, pus, surgical wounds and tissues, ear, bone marrow, gastric, skin, sinus, eye, conjunctiva, cornea, drainage, open wounds, lesion, burn, decubitus, incision, ulcer, and genital cultures.

For other requests, order specific culture (e.g. C AFB for Mycobacterial culture). C MISC ANA must also be ordered for anaerobic culture. Requisition must state specific site of collection for culture.Write in suspected organism to ensure appropriate plating and incubation.

If orbital cellulitis is present or suspected, communicate this to the laboratory.

Please indicate if Actinomyces is suspected. Actinomyces has been reported in endometritis and pelvic infections associated with IUDs.

For catheter tips, please note specific catheter type on requisition. Catheter tips should be submitted simultaneously with blood cultures.

To screen for Neisseria gonorrhoea only in genital cultures, please submit specimen for DNA probe.To screen for Group B Strep, order C GBS.

Test includes a direct Gram stain on all abscesses, aspirates, amniotic fluid, tissue, and Copan eSwabs in transport media. Gram stain is performed by request for other miscellaneous sites.

Sensitivity testing is performed on aerobic pathogens, if recovered.

Performed by

PCL Clinical Laboratory - Microbiology

Notes

Gram stain, identification, and susceptibility tests are billed separately from the culture.

Performed

Daily

Methodology

Aerobic culture

Reported

Varies

Synonyms

  • LAB3274

Interpretive Data

Positive gram stains on tissues or body fluids will be reported to the physician immediately. Positive cultures on tissues or body fluids will be reported to the caregiver on the same day that the results are available.

Negative results are reported at 48 hours with broth held for 7 days prior to discarding on cultures warranting anaerobic culture.

Quantitative burn cultures include quantitative counts of aerobic organisms of skin and tissue specimens from burn patients.

Performed by

PCL Clinical Laboratory - Microbiology

CPT Codes

87070

Collection

LAB3274

Collect

In a sterile container: pus from aspiration, bone marrow aspirate, corneal scrapings, wound exudate, drainage fluids, small pieces of tissue or biopsy, venous or arterial catheter tip, bile, gall bladder fluid, prostate tissue or fluid, placenta tissue, or genital swab; or

Copan eSwab in transport media. Swabs are not acceptable for tissue culture. Fluid, pus, or drainage is ALWAYS preferable to a swab.

For corneal and conjunctiva cultures, media is available in the laboratory if bedside inoculation is desired.

Pediatric Collection

In a sterile container, 0.5-2 mL fluid or 0.1 g viable tissue for quantitative burn cultures; or Copan eSwab in transport media.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; delayed transport to the laboratory, tissue in fixative, urinary catheters, grossly contaminated specimen. Swabs are not acceptable for tissue culture.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport ASAP at ambient temperature.

Stability (from collection to initiation)

Ambient (not in transport media): 1 hour. Ambient (in transport media): greater than 1 hour.

Remarks

Routine aerobic bacterial culture only. Miscellaneous cultures include abscess, aspirate, catheter tips, deep wounds, pus, surgical wounds and tissues, ear, bone marrow, gastric, skin, sinus, eye, conjunctiva, cornea, drainage, open wounds, lesion, burn, decubitus, incision, ulcer, and genital cultures.

For other requests, order specific culture (e.g. C AFB for Mycobacterial culture). C MISC ANA must also be ordered for anaerobic culture. Requisition must state specific site of collection for culture.Write in suspected organism to ensure appropriate plating and incubation.

If orbital cellulitis is present or suspected, communicate this to the laboratory.

Please indicate if Actinomyces is suspected. Actinomyces has been reported in endometritis and pelvic infections associated with IUDs.

For catheter tips, please note specific catheter type on requisition. Catheter tips should be submitted simultaneously with blood cultures.

To screen for Neisseria gonorrhoea only in genital cultures, please submit specimen for DNA probe.To screen for Group B Strep, order C GBS.

Test includes a direct Gram stain on all abscesses, aspirates, amniotic fluid, tissue, and Copan eSwabs in transport media. Gram stain is performed by request for other miscellaneous sites.

Sensitivity testing is performed on aerobic pathogens, if recovered.

Performed by

PCL Clinical Laboratory - Microbiology

Notes

Gram stain, identification, and susceptibility tests are billed separately from the culture.

Ordering

Performed

Daily

Methodology

Aerobic culture

Reported

Varies

Synonyms

  • LAB3274
Result Interpretation

Interpretive Data

Positive gram stains on tissues or body fluids will be reported to the physician immediately. Positive cultures on tissues or body fluids will be reported to the caregiver on the same day that the results are available.

Negative results are reported at 48 hours with broth held for 7 days prior to discarding on cultures warranting anaerobic culture.

Quantitative burn cultures include quantitative counts of aerobic organisms of skin and tissue specimens from burn patients.

Performed by

PCL Clinical Laboratory - Microbiology

Administrative

CPT Codes

87070

MISCELLANEOUS CULTURE, ANAEROBIC

LAB3278

 

Collect

In a sterile container: pus from aspiration, bone marrow aspirate, corneal scrapings, wound exudate, drainage fluids, small pieces of tissue or biopsy, bile, gall bladder fluid, prostate tissue or fluid;

or

Copan e-swab with transport media.

Fluid, pus, or drainage are ALWAYS preferred to swabs.

For corneal and conjunctiva cultures, media is available in the laboratory if bedside inoculation is desired.

Pediatric Collection

Sterile container with 0.5 - 2 mL fluid or 0.1 g viable tissue for quantitative burn cultures, or Copan e-swab in transport media.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; delayed transport to the laboratory; tissue in fixative; grossly contaminated specimen. Swabs are not acceptable for tissue culture. Anaerobic cultures will not be performed on catheters, vaginal, or cervical sites.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send ASAP at ambient temperature.

Stability (from collection to initiation)

Ambient (not in transport media): 1 hour; Ambient (in transport media): Greater than 1 hour.

Remarks

Routine anaerobic bacterial culture only. Miscellaneous anaerobic cultures include abscess, aspirate, deep wounds, pus, surgical wounds and tissues, ear, bone marrow, gastric, skin, sinus, eye, conjunctiva, cornea, drainage, open wounds, lesion, burn, decubitus, incision, and ulcer.

Requisition must state specific site of collection for culture. Write in suspected organism to ensure appropriate plating and incubation. If orbital cellulitis is present or suspected, communicate this to the laboratory.

Miscellaneous anaerobic cultures include abscess, aspirate, deep wounds, pus, surgical wounds and tissues, ear, bone marrow, gastric, skin, sinus, eye, conjunctiva, cornea, drainage, open wounds, lesion, burn, decubitus, incision, and ulcer.

Test includes a direct Gram stain on all abscesses, aspirates, amniotic fluid, tissue, and Copan e-swabs in transport media. Gram stain is performed by request for other miscellaneous sites.

Performed by

PCL Clinical Laboratory - Microbiology

Performed

Daily

Methodology

Anaerobic Culture

Reported

Varies

Synonyms

  • LAB3278

Interpretive Data

Quantitative burn culture includes quantitative counts of aerobic organisms of skin and tissue specimens from burn patients.

Positive gram stains on tissues or body fluids will be reported to the physician immediately. Positive cultures on tissues or body fluids will be reported to the caregiver on the same day that results are available.

Negative results are reported at 48 hours with broth held for 7 days prior to discarding on cultures warranting anaerobic culture.

Performed by

PCL Clinical Laboratory - Microbiology

CPT Codes

87075

Collection

LAB3278

 

Collect

In a sterile container: pus from aspiration, bone marrow aspirate, corneal scrapings, wound exudate, drainage fluids, small pieces of tissue or biopsy, bile, gall bladder fluid, prostate tissue or fluid;

or

Copan e-swab with transport media.

Fluid, pus, or drainage are ALWAYS preferred to swabs.

For corneal and conjunctiva cultures, media is available in the laboratory if bedside inoculation is desired.

Pediatric Collection

Sterile container with 0.5 - 2 mL fluid or 0.1 g viable tissue for quantitative burn cultures, or Copan e-swab in transport media.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; delayed transport to the laboratory; tissue in fixative; grossly contaminated specimen. Swabs are not acceptable for tissue culture. Anaerobic cultures will not be performed on catheters, vaginal, or cervical sites.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send ASAP at ambient temperature.

Stability (from collection to initiation)

Ambient (not in transport media): 1 hour; Ambient (in transport media): Greater than 1 hour.

Remarks

Routine anaerobic bacterial culture only. Miscellaneous anaerobic cultures include abscess, aspirate, deep wounds, pus, surgical wounds and tissues, ear, bone marrow, gastric, skin, sinus, eye, conjunctiva, cornea, drainage, open wounds, lesion, burn, decubitus, incision, and ulcer.

Requisition must state specific site of collection for culture. Write in suspected organism to ensure appropriate plating and incubation. If orbital cellulitis is present or suspected, communicate this to the laboratory.

Miscellaneous anaerobic cultures include abscess, aspirate, deep wounds, pus, surgical wounds and tissues, ear, bone marrow, gastric, skin, sinus, eye, conjunctiva, cornea, drainage, open wounds, lesion, burn, decubitus, incision, and ulcer.

Test includes a direct Gram stain on all abscesses, aspirates, amniotic fluid, tissue, and Copan e-swabs in transport media. Gram stain is performed by request for other miscellaneous sites.

Performed by

PCL Clinical Laboratory - Microbiology

Ordering

Performed

Daily

Methodology

Anaerobic Culture

Reported

Varies

Synonyms

  • LAB3278
Result Interpretation

Interpretive Data

Quantitative burn culture includes quantitative counts of aerobic organisms of skin and tissue specimens from burn patients.

Positive gram stains on tissues or body fluids will be reported to the physician immediately. Positive cultures on tissues or body fluids will be reported to the caregiver on the same day that results are available.

Negative results are reported at 48 hours with broth held for 7 days prior to discarding on cultures warranting anaerobic culture.

Performed by

PCL Clinical Laboratory - Microbiology

Administrative

CPT Codes

87075

MITOCHONDRIAL DISORDERS (mtDNA) SEQUENCING

LAB3758

Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B). Peripheral blood required.

Specimen Preparation

Transport 3 mL whole blood. (Min: 1 mL)

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable

Remarks

Submit the Patient History Form for Mitochondrial Disorders Studies with the Electronic Packing List.

Performed by

Medical Genetics Laboratories - Baylor College of Medicine

Performed

Varies

Methodology

Massively Parallel Sequencing

Reported

Within 12 weeks

Synonyms

  • LHON
  • MELAS
  • MERRF
  • NARP
  • MITOCHONDRIAL DNA SCREEN
  • MT-ATP6, MT-ATP8, MT-CO1, MT-CO2, MT-CO3, MT-CYB, MT-ND1, MT-ND2, MT-ND3, MT-ND4, MT-ND4L, MT-ND5
  • MT-ND6, MT-RNR1, MT-RNR2, MT-TA, MT-TC, MT-TD, MT-TE, MT-TF, MT-TG, MT-TH, MT-TI, MT-TK, MT-TL1
  • mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS)
  • Leigh syndrome
  • myoclonic epilepsy with ragged red fibers (MERRF)
  • mtDNA sequencing
  • progressive external ophthalmoplegia (PEO)
  • neurogenic weakness with ataxia and retinitis pigmentosa (NARP)
  • MT-TL2, MT-TM, MT-TN,MT-TP, MT-TQ, MT-TR, MT-TS1, MT-TS2, MT-TT, MT-TV, MT-TW, MT-TY
  • Leber hereditary optic neuropathy (LHON)
  • LAB3758
  • MITOCHONDRIA
  • MITODNASCR

Ordering Recommendations

Assess for sequence variants in the mitochondrial genome (mtDNA) causing mitochondrial disorders.

Reference Interval

By report

Interpretive Data

Refer to report.

Performed by

Medical Genetics Laboratories - Baylor College of Medicine

CPT Codes

81460
Collection

LAB3758

Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B). Peripheral blood required.

Specimen Preparation

Transport 3 mL whole blood. (Min: 1 mL)

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable

Remarks

Submit the Patient History Form for Mitochondrial Disorders Studies with the Electronic Packing List.

Performed by

Medical Genetics Laboratories - Baylor College of Medicine

Ordering

Performed

Varies

Methodology

Massively Parallel Sequencing

Reported

Within 12 weeks

Synonyms

  • LHON
  • MELAS
  • MERRF
  • NARP
  • MITOCHONDRIAL DNA SCREEN
  • MT-ATP6, MT-ATP8, MT-CO1, MT-CO2, MT-CO3, MT-CYB, MT-ND1, MT-ND2, MT-ND3, MT-ND4, MT-ND4L, MT-ND5
  • MT-ND6, MT-RNR1, MT-RNR2, MT-TA, MT-TC, MT-TD, MT-TE, MT-TF, MT-TG, MT-TH, MT-TI, MT-TK, MT-TL1
  • mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS)
  • Leigh syndrome
  • myoclonic epilepsy with ragged red fibers (MERRF)
  • mtDNA sequencing
  • progressive external ophthalmoplegia (PEO)
  • neurogenic weakness with ataxia and retinitis pigmentosa (NARP)
  • MT-TL2, MT-TM, MT-TN,MT-TP, MT-TQ, MT-TR, MT-TS1, MT-TS2, MT-TT, MT-TV, MT-TW, MT-TY
  • Leber hereditary optic neuropathy (LHON)
  • LAB3758
  • MITOCHONDRIA
  • MITODNASCR

Ordering Recommendations

Assess for sequence variants in the mitochondrial genome (mtDNA) causing mitochondrial disorders.
Result Interpretation

Reference Interval

By report

Interpretive Data

Refer to report.

Performed by

Medical Genetics Laboratories - Baylor College of Medicine

Administrative

CPT Codes

81460

MITOTANE LEVEL

LAB5197

Collect

6.0 mL Red top tube (no gel)

6.0 mL Dark Green (no gel) is acceptable.

Specimen Preparation

Spin, separate and refrigerate.

Pediatric Collection

Minimum volume = 0.3 mL serum/plasma

Unacceptable Conditions

Tubes with gel.

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

Ambient:  72 hours

Refrigerated:  14 days

Frozen:  180 days

Remarks

.

Performed by

Medtox Laboratories, Inc. in St. Paul, MN.

Performed

Monday-Sunday

Methodology

Gas Chromatography/Flame Ionization Detection (GC-FID)

Reported

5-9 days.

Synonyms

  • LAB5197
  • MITOTANE

Interpretive Data

Therapeutic and toxic ranges not established.

Usual therapeutic doses produce Mitotane serum concentrations of less thatn 100 ug/mL

Performed by

Medtox Laboratories, Inc. in St. Paul, MN.

CPT Codes

82491

Collection

LAB5197

Collect

6.0 mL Red top tube (no gel)

6.0 mL Dark Green (no gel) is acceptable.

Specimen Preparation

Spin, separate and refrigerate.

Pediatric Collection

Minimum volume = 0.3 mL serum/plasma

Unacceptable Conditions

Tubes with gel.

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

Ambient:  72 hours

Refrigerated:  14 days

Frozen:  180 days

Remarks

.

Performed by

Medtox Laboratories, Inc. in St. Paul, MN.

Ordering

Performed

Monday-Sunday

Methodology

Gas Chromatography/Flame Ionization Detection (GC-FID)

Reported

5-9 days.

Synonyms

  • LAB5197
  • MITOTANE
Result Interpretation

Interpretive Data

Therapeutic and toxic ranges not established.

Usual therapeutic doses produce Mitotane serum concentrations of less thatn 100 ug/mL

Performed by

Medtox Laboratories, Inc. in St. Paul, MN.

Administrative

CPT Codes

82491

MIXED ALCOHOLS

LAB3044

ALCOHOLS MIX

Collect

One 6.0 mL red top, no gel, serum (clot activator).

Patient Preparation

Do NOT use alcohol-based wipes to cleanse the skin prior to venipuncture.

Pediatric Collection

Minimum volume: 1 mL serum.

Unacceptable Conditions

SST or PST gel tubes; specimens drawn in capillary tubes; insufficient volume; sample not properly identified; forensic samples.

Storage/Transport Temperature

Internal: Deliver to lab within one hour of collection.
Offsite: Allow to clot for 30 minutes after collection. Centrifuge within one hour of collection. Place at least 1 mL serum in a tightly sealed aliquot container with minimal dead space and transport to lab at 2-8 degrees C (refrigerated or on ice).

Stability (from collection to initiation)

Prior to centrifugation: Ambient, 1 hour

Tightly capped serum aliquot: Ambient, 1 week; Refrigerated, 2 weeks; Frozen (-20 degrees C), 1 month.

Remarks

Do not use alcohol prior to venipuncture. Specimen should be kept closed as much as possible and should be analyzed as soon as possible after collection. If prolonged storage is anticipated, the addition of fluoride (collect in a grey top tube) is recommended.

Performed by

PCL Clinical Laboratory - Toxicology

Performed

Daily

Methodology

Gas chromatography (GC).

Reported

Same day

Synonyms

  • LAB3044

Reference Interval

Ethanol <5 mg/dL

Methanol <5 mg/dL

Isopropanol <5 mg/dL

Performed by

PCL Clinical Laboratory - Toxicology

CPT Codes

84600

Collection

LAB3044

ALCOHOLS MIX

Collect

One 6.0 mL red top, no gel, serum (clot activator).

Patient Preparation

Do NOT use alcohol-based wipes to cleanse the skin prior to venipuncture.

Pediatric Collection

Minimum volume: 1 mL serum.

Unacceptable Conditions

SST or PST gel tubes; specimens drawn in capillary tubes; insufficient volume; sample not properly identified; forensic samples.

Storage/Transport Temperature

Internal: Deliver to lab within one hour of collection.
Offsite: Allow to clot for 30 minutes after collection. Centrifuge within one hour of collection. Place at least 1 mL serum in a tightly sealed aliquot container with minimal dead space and transport to lab at 2-8 degrees C (refrigerated or on ice).

Stability (from collection to initiation)

Prior to centrifugation: Ambient, 1 hour

Tightly capped serum aliquot: Ambient, 1 week; Refrigerated, 2 weeks; Frozen (-20 degrees C), 1 month.

Remarks

Do not use alcohol prior to venipuncture. Specimen should be kept closed as much as possible and should be analyzed as soon as possible after collection. If prolonged storage is anticipated, the addition of fluoride (collect in a grey top tube) is recommended.

Performed by

PCL Clinical Laboratory - Toxicology

Ordering

Performed

Daily

Methodology

Gas chromatography (GC).

Reported

Same day

Synonyms

  • LAB3044
Result Interpretation

Reference Interval

Ethanol <5 mg/dL

Methanol <5 mg/dL

Isopropanol <5 mg/dL

Performed by

PCL Clinical Laboratory - Toxicology

Administrative

CPT Codes

84600

MPL CODON 515 MUTATION QNT PCR

LAB4417

Collect

Lavender (EDTA).

Specimen Preparation

Transport 5 mL whole blood or bone marrow. (Min: 1 mL)

Unacceptable Conditions

Plasma or serum. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable

Notes

The test will detect and quantify MPL codon 515 mutation, including W515K and W515L.

Performed

DNA isolation: Sun-Sat
Assay: Varies

Methodology

Polymerase Chain Reaction/Quantitative Pyrosequencing

Reported

7-12 days

Synonyms

  • LAB4417
  • MPLMUTQPCR
  • MPL

Additional Technical Information

Ordering Recommendations

May be useful when essential thrombocythemia or idiopathic myelofibrosis is suspected in JAK2 V617F-negative individuals.

Interpretive Data

Refer to report

CPT Codes

81402
Collection

LAB4417

Collect

Lavender (EDTA).

Specimen Preparation

Transport 5 mL whole blood or bone marrow. (Min: 1 mL)

Unacceptable Conditions

Plasma or serum. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable

Notes

The test will detect and quantify MPL codon 515 mutation, including W515K and W515L.
Ordering

Performed

DNA isolation: Sun-Sat
Assay: Varies

Methodology

Polymerase Chain Reaction/Quantitative Pyrosequencing

Reported

7-12 days

Synonyms

  • LAB4417
  • MPLMUTQPCR
  • MPL

Additional Technical Information

Ordering Recommendations

May be useful when essential thrombocythemia or idiopathic myelofibrosis is suspected in JAK2 V617F-negative individuals.
Result Interpretation

Interpretive Data

Refer to report

Administrative

CPT Codes

81402

MRSA SCREEN

LAB3448

 

Collect

Preferred: collect single swab of nares, axilla, groin and place in eSwab transport media.Caution, order of collection must be nares to groin!

 Accepted:           

  • Individual swabs of nares, axilla and groin in eSwab transport media
  • Swab of original site of infection using eSwab transport media (if still available)
  • Individual swabs of nares, axilla and groin in Culturettes.

Unacceptable Conditions

Specimen source and/or suspected organism not indicated; Specimen not properly identified; dry swab; calcium alginate swab.

Storage/Transport Temperature

Internal: Deliver to lab imediately at ambient temperature.

Offsite: Submit in transport media up to 24 hours at ambient temperature, or up to 72 hours refrigerated.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable.

Remarks

For guidelines for when to collect specimens, how and when to place patients in isolation and how to clear a patient from precautions, please refer to the “Isolation/Transmission Based Precautions Policy” under Policies and Procedures – Infection Control on the HUB.

Performed by

PCL Clinical Laboratory - Microbiology

Notes

Specimens are held and batched between 1800 and 0700. Specimens received after 1800 daily will be plated the following morning at 0700.

Performed

Daily

Methodology

Chrome agar

Reported

24 - 48 hours

Synonyms

  • Methicillin Resistant Staphylococcus aureus Screen
  • LAB3448

Ordering Recommendations

See “Isolation/Transmission Based Precautions Policy”/

Performed by

PCL Clinical Laboratory - Microbiology

CPT Codes

87081

Collection

LAB3448

 

Collect

Preferred: collect single swab of nares, axilla, groin and place in eSwab transport media.Caution, order of collection must be nares to groin!

 Accepted:           

  • Individual swabs of nares, axilla and groin in eSwab transport media
  • Swab of original site of infection using eSwab transport media (if still available)
  • Individual swabs of nares, axilla and groin in Culturettes.

Unacceptable Conditions

Specimen source and/or suspected organism not indicated; Specimen not properly identified; dry swab; calcium alginate swab.

Storage/Transport Temperature

Internal: Deliver to lab imediately at ambient temperature.

Offsite: Submit in transport media up to 24 hours at ambient temperature, or up to 72 hours refrigerated.

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable.

Remarks

For guidelines for when to collect specimens, how and when to place patients in isolation and how to clear a patient from precautions, please refer to the “Isolation/Transmission Based Precautions Policy” under Policies and Procedures – Infection Control on the HUB.

Performed by

PCL Clinical Laboratory - Microbiology

Notes

Specimens are held and batched between 1800 and 0700. Specimens received after 1800 daily will be plated the following morning at 0700.

Ordering

Performed

Daily

Methodology

Chrome agar

Reported

24 - 48 hours

Synonyms

  • Methicillin Resistant Staphylococcus aureus Screen
  • LAB3448

Ordering Recommendations

See “Isolation/Transmission Based Precautions Policy”/

Result Interpretation

Performed by

PCL Clinical Laboratory - Microbiology

Administrative

CPT Codes

87081

MTHFR COMBO MUTATIONS

LAB3684

 

Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).

Specimen Preparation

Transport 3 mL whole blood. (Min: 1 mL)

Unacceptable Conditions

Plasma or serum. Heparinized specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 72 hours; Refrigerated: 2 weeks; Frozen: 1 month

Performed

Sun-Sat

Methodology

Polymerase Chain Reaction/High Resolution Melt Analysis

Reported

2-6 days

Synonyms

  • LAB3684
  • MTHFR PCR
  • MTHFR

Additional Technical Information

Ordering Recommendations

Determine genetic cause for hyperhomocysteinemia and potential sensitivity to antifolate drugs. Test is not recommended for women who have recurrent pregnancy loss, thrombophilia screening, or neural tube defect risk assessment.

Reference Interval

Negative: Neither of the common MTHFR gene variants tested, c.665C>T (previously designated C677T) and c.1286A>C (previously designated A1298C), were detected. Other causes of elevated homocysteine levels, coronary heart disease, or thrombosis were not assessed. This genotype is associated with a normal folate metabolism.

Interpretive Data

Background Information for Methylenetetrahydrofolate Reductase (MTHFR) 2 Variants:
Characteristics: Variants in the MTHFR gene (c.665C>T and c.1286A>C) correlate with reduced enzyme activity; however, only homozygotes for the c.665C>T variant have been significantly associated with elevated plasma homocysteine levels and with an increased risk for premature cardiovascular disease. These individuals may also show toxicity from medications (ie, methotrexate) that affect folate metabolism. 
Incidence: The allele frequency of c.665C>T is 0.35 in European Caucasians and 0.12 in African Americans. The allele frequency of c.1286A>C is 0.31 in European Caucasians and 0.15 in African Americans.
Inheritance: Autosomal recessive.
Cause: Homozygosity for MTHFR gene mutation c.665C>T.
Mutations Tested: c.665C>T (previously designated C677T); p.Ala222Val and c.1286A>C (previously designated A1298C); p.Glu429Ala.
Clinical Sensitivity: Undefined. Sensitivity is dependent upon multiple contributing factors.
Methodology: Polymerase chain reaction followed by high resolution melt analysis.
Analytical Sensitivity & Specificity: 99 percent.
Limitations: Only the two MTHFR gene mutations (c.665C>T and c.1286A>C) will be targeted. Diagnostic errors can occur due to rare sequence variations.

CPT Codes

81291
Collection

LAB3684

 

Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).

Specimen Preparation

Transport 3 mL whole blood. (Min: 1 mL)

Unacceptable Conditions

Plasma or serum. Heparinized specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 72 hours; Refrigerated: 2 weeks; Frozen: 1 month
Ordering

Performed

Sun-Sat

Methodology

Polymerase Chain Reaction/High Resolution Melt Analysis

Reported

2-6 days

Synonyms

  • LAB3684
  • MTHFR PCR
  • MTHFR

Additional Technical Information

Ordering Recommendations

Determine genetic cause for hyperhomocysteinemia and potential sensitivity to antifolate drugs. Test is not recommended for women who have recurrent pregnancy loss, thrombophilia screening, or neural tube defect risk assessment.
Result Interpretation

Reference Interval

Negative: Neither of the common MTHFR gene variants tested, c.665C>T (previously designated C677T) and c.1286A>C (previously designated A1298C), were detected. Other causes of elevated homocysteine levels, coronary heart disease, or thrombosis were not assessed. This genotype is associated with a normal folate metabolism.

Interpretive Data

Background Information for Methylenetetrahydrofolate Reductase (MTHFR) 2 Variants:
Characteristics: Variants in the MTHFR gene (c.665C>T and c.1286A>C) correlate with reduced enzyme activity; however, only homozygotes for the c.665C>T variant have been significantly associated with elevated plasma homocysteine levels and with an increased risk for premature cardiovascular disease. These individuals may also show toxicity from medications (ie, methotrexate) that affect folate metabolism. 
Incidence: The allele frequency of c.665C>T is 0.35 in European Caucasians and 0.12 in African Americans. The allele frequency of c.1286A>C is 0.31 in European Caucasians and 0.15 in African Americans.
Inheritance: Autosomal recessive.
Cause: Homozygosity for MTHFR gene mutation c.665C>T.
Mutations Tested: c.665C>T (previously designated C677T); p.Ala222Val and c.1286A>C (previously designated A1298C); p.Glu429Ala.
Clinical Sensitivity: Undefined. Sensitivity is dependent upon multiple contributing factors.
Methodology: Polymerase chain reaction followed by high resolution melt analysis.
Analytical Sensitivity & Specificity: 99 percent.
Limitations: Only the two MTHFR gene mutations (c.665C>T and c.1286A>C) will be targeted. Diagnostic errors can occur due to rare sequence variations.

Administrative

CPT Codes

81291

MUCOPOLYSACCHARIDES - URINE

LAB1058

 

Collect

Urine.

Patient Preparation

Morning void preferred.

Specimen Preparation

Freeze specimen immediately. Transport 20 mL urine. (Min: 10 mL)

Unacceptable Conditions

Specimens containing preservatives.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month (avoid repeated freeze/thaw cycles)

Performed

Tue

Methodology

Electrophoresis/Spectrophotometry

Reported

7 - 10 days

Synonyms

  • LAB1058
  • MPS SCREEN
  • MPS

Ordering Recommendations

Use to evaluate symptomatic patients for mucopolysaccharidoses (MPS). To monitor glycosaminoglycans (GAGs) in patients previously diagnosed with MPS, refer to Mucopolysaccharides, Quantitative, Urine (0081357).

Reference Interval

Components
Reference Interval
MPS ElectrophoresisBy report
Mucopolysaccharides, Quantitative, Urine0-5 months: 14.6-47.8 mg/mmol creatinine
6-11 months: 3.7-35.5 mg/mmol creatinine
1-2 years: 5.4-30.8 mg/mmol creatinine
3-6 years: 5.2-16.7 mg/mmol creatinine
7-13 years: 2.4-10.2 mg/mmol creatinine
14 years or older: 0.0-7.1 mg/mmol creatinine

Interpretive Data

Mucopolysaccharides (Glycosaminoglycans) include:  Keratan Sulfate, Heparan Sulfate, Dermatan Sulfate, and Chondroitin Sulfates 4 and 6.  The excretion of Heparan Sulfate is variable.  A normal mucopolysaccharides screen does not exclude Sanfilippo Syndrome (Mucopolysaccharidosis Type III).

CPT Codes

82664; 83864
Collection

LAB1058

 

Collect

Urine.

Patient Preparation

Morning void preferred.

Specimen Preparation

Freeze specimen immediately. Transport 20 mL urine. (Min: 10 mL)

Unacceptable Conditions

Specimens containing preservatives.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month (avoid repeated freeze/thaw cycles)
Ordering

Performed

Tue

Methodology

Electrophoresis/Spectrophotometry

Reported

7 - 10 days

Synonyms

  • LAB1058
  • MPS SCREEN
  • MPS

Ordering Recommendations

Use to evaluate symptomatic patients for mucopolysaccharidoses (MPS). To monitor glycosaminoglycans (GAGs) in patients previously diagnosed with MPS, refer to Mucopolysaccharides, Quantitative, Urine (0081357).
Result Interpretation

Reference Interval

Components
Reference Interval
MPS ElectrophoresisBy report
Mucopolysaccharides, Quantitative, Urine0-5 months: 14.6-47.8 mg/mmol creatinine
6-11 months: 3.7-35.5 mg/mmol creatinine
1-2 years: 5.4-30.8 mg/mmol creatinine
3-6 years: 5.2-16.7 mg/mmol creatinine
7-13 years: 2.4-10.2 mg/mmol creatinine
14 years or older: 0.0-7.1 mg/mmol creatinine

Interpretive Data

Mucopolysaccharides (Glycosaminoglycans) include:  Keratan Sulfate, Heparan Sulfate, Dermatan Sulfate, and Chondroitin Sulfates 4 and 6.  The excretion of Heparan Sulfate is variable.  A normal mucopolysaccharides screen does not exclude Sanfilippo Syndrome (Mucopolysaccharidosis Type III).

Administrative

CPT Codes

82664; 83864

MUMPS ANTIBODY IGM

LAB161

 

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, hemolyzed, heat-inactivated, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Mon-Fri

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-5 days

Synonyms

  • LAB161
  • MUMPSM
  • MUM

Ordering Recommendations

Aid in the diagnosis of suspected mumps infection.

Reference Interval

0.79 IV or less: Negative - No significant level of detectable IgM antibody to Mumps virus.
0.80-1.20 IV: Equivocal - Borderline levels of IgM antibody to Mumps virus. Repeat testing in 10-14 days may be helpful.
1.21 IV or greater: Positive - Presence of IgM antibody to Mumps virus detected, which may indicate a current or recent infection. However, low levels of IgM antibody may occasionally persist for more than 12 months post-infection or immunization.

CPT Codes

86735
Collection

LAB161

 

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, hemolyzed, heat-inactivated, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Mon-Fri

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Reported

1-5 days

Synonyms

  • LAB161
  • MUMPSM
  • MUM

Ordering Recommendations

Aid in the diagnosis of suspected mumps infection.
Result Interpretation

Reference Interval

0.79 IV or less: Negative - No significant level of detectable IgM antibody to Mumps virus.
0.80-1.20 IV: Equivocal - Borderline levels of IgM antibody to Mumps virus. Repeat testing in 10-14 days may be helpful.
1.21 IV or greater: Positive - Presence of IgM antibody to Mumps virus detected, which may indicate a current or recent infection. However, low levels of IgM antibody may occasionally persist for more than 12 months post-infection or immunization.
Administrative

CPT Codes

86735

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