LUTEINIZING HORMONE

LAB87

LH

 

Collect

One 4.5 mL Gold top tube with gel separator (SST). clot activator serum.

Pediatric Collection

1.5 mL Blood.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; sample has been thawed and refrozen.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Allow to clot for 30 minutes prior to centrifugation. Centrifuge within 2 hours of collection. Send at 2-8 degrees C.

Stability (from collection to initiation)

After centrifugation:

Ambient, 8 hours; Refrigerated, 2 days; Frozen, Unacceptable

Serum aliquot:

Ambient, 8 hours; Refrigerated, 2 days; Frozen (-20 degrees C), Unknown.

Samples that have been thawed and refrozen are not suitable for LH analysis

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Chemiluminescent Immunoassay

Reported

Daily

Synonyms

  • LAB87
  • LH

Interpretive Data

Reporting units mIU/mL

Females

Follicular phase 1.9-12.5

Midcycle 8.7-76.3

Luteal phase 0.5-16.9

Pregnant <1.5

Postmenopausal 15.9-54.0

Males

20-70 years 1.5-9.3

>70 years 3.1-34.6

Children

0.0-6.0

Performed by

PCL Clinical Laboratory - Core

CPT Codes

83002

Collection

LAB87

LH

 

Collect

One 4.5 mL Gold top tube with gel separator (SST). clot activator serum.

Pediatric Collection

1.5 mL Blood.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; sample has been thawed and refrozen.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Allow to clot for 30 minutes prior to centrifugation. Centrifuge within 2 hours of collection. Send at 2-8 degrees C.

Stability (from collection to initiation)

After centrifugation:

Ambient, 8 hours; Refrigerated, 2 days; Frozen, Unacceptable

Serum aliquot:

Ambient, 8 hours; Refrigerated, 2 days; Frozen (-20 degrees C), Unknown.

Samples that have been thawed and refrozen are not suitable for LH analysis

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Chemiluminescent Immunoassay

Reported

Daily

Synonyms

  • LAB87
  • LH
Result Interpretation

Interpretive Data

Reporting units mIU/mL

Females

Follicular phase 1.9-12.5

Midcycle 8.7-76.3

Luteal phase 0.5-16.9

Pregnant <1.5

Postmenopausal 15.9-54.0

Males

20-70 years 1.5-9.3

>70 years 3.1-34.6

Children

0.0-6.0

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

83002

LYMPHOCYTE ENUMERATION OF TBNK

LAB5089

TBNK

Collect

One 4 or 6 mL Green top tube (sodium heparin) AND one 4 mL Purple top tube (EDTA). 

Dark Green and EDTA must be collected at same draw.

Pediatric Collection

3 mL Green top tube (sodium heparin) AND 3 mL Purple top tube (EDTA) or microtainer (EDTA).

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; specimens older than 24 hours; clotted, frozen, or hemolyzed specimens.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport at ambient temperature.

Stability (from collection to initiation)

Ambient: Green top/purple top tubes for lymphocyte subset analysis 24 hours; Purple top tube for CBC 8 hours.

 

Refrigerated: Purple top tube for CBC 36 hours.

Remarks

TBNK panel requires an Absolute Lymphocyte Count. A Complete Blood Count (CBC) and differential (DIFF) must be ordered. CPT code 85025 or 85027 and 85007 will be billed as needed.

Performed by

PCL Clinical Laboratory - Flow Cytometry

Performed

Daily

Methodology

Flow Cytometry

Reported

Same day

Synonyms

  • Natural Killer Cells Percent & Absolute
  • CD56 Percent & Absolute
  • LAB3006
  • TXIP
  • TBNK

Reference Interval

Component Sex From Age To Age Normal Low Normal High Units
TX CD3 Percent M/F 18 150 46 86 %
TX CD3 Absolute M/F 18 150 708 2790 /uL
TX CD4 Percent M/F 18 150 25 62 %
TX CD4 Absolute M/F 18 150 425 1860 /uL
TX CD 8 Percent M/F 18 150 14 40 %
TX CD 8 Absolute M/F 18 150 216 1292 /uL
TX CD4/CD8 Ratio M/F 0 150 1.0 3.3 %
TX CD19 Percent M/F 18 150 5 25 %
TX CD19 Absolute M/F 18 150 90 1170 /uL
TX CD16+56 Percent M/F 18 150 5 32 %
TX CD16+56 Absolute M/F 18 150 84 1064 /uL

Performed by

PCL Clinical Laboratory - Flow Cytometry

CPT Codes

86355, 86356 x2, 86357, 86360, 86359

Collection

LAB5089

TBNK

Collect

One 4 or 6 mL Green top tube (sodium heparin) AND one 4 mL Purple top tube (EDTA). 

Dark Green and EDTA must be collected at same draw.

Pediatric Collection

3 mL Green top tube (sodium heparin) AND 3 mL Purple top tube (EDTA) or microtainer (EDTA).

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; specimens older than 24 hours; clotted, frozen, or hemolyzed specimens.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Transport at ambient temperature.

Stability (from collection to initiation)

Ambient: Green top/purple top tubes for lymphocyte subset analysis 24 hours; Purple top tube for CBC 8 hours.

 

Refrigerated: Purple top tube for CBC 36 hours.

Remarks

TBNK panel requires an Absolute Lymphocyte Count. A Complete Blood Count (CBC) and differential (DIFF) must be ordered. CPT code 85025 or 85027 and 85007 will be billed as needed.

Performed by

PCL Clinical Laboratory - Flow Cytometry

Ordering

Performed

Daily

Methodology

Flow Cytometry

Reported

Same day

Synonyms

  • Natural Killer Cells Percent & Absolute
  • CD56 Percent & Absolute
  • LAB3006
  • TXIP
  • TBNK
Result Interpretation

Reference Interval

Component Sex From Age To Age Normal Low Normal High Units
TX CD3 Percent M/F 18 150 46 86 %
TX CD3 Absolute M/F 18 150 708 2790 /uL
TX CD4 Percent M/F 18 150 25 62 %
TX CD4 Absolute M/F 18 150 425 1860 /uL
TX CD 8 Percent M/F 18 150 14 40 %
TX CD 8 Absolute M/F 18 150 216 1292 /uL
TX CD4/CD8 Ratio M/F 0 150 1.0 3.3 %
TX CD19 Percent M/F 18 150 5 25 %
TX CD19 Absolute M/F 18 150 90 1170 /uL
TX CD16+56 Percent M/F 18 150 5 32 %
TX CD16+56 Absolute M/F 18 150 84 1064 /uL

Performed by

PCL Clinical Laboratory - Flow Cytometry

Administrative

CPT Codes

86355, 86356 x2, 86357, 86360, 86359

LYMPHOCYTE FUNCTION TESTING

LAB3647

Collect

10 ml Green (sodium heparin) - specimens must be collected within 24 hours of test performance. SAMPLES CAN BE SENT MON-THURS AND FRIDAY UNTIL NOON.

Specimen Preparation

Transport 30 mL whole blood  in original collection tubes. (Min: 10 mL)

Do not centrifuge, refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
 

Pediatric Collection

Infant Minimum: 10mL

Unacceptable Conditions

Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 24 hours.

Storage/Transport Temperature

Critical Room Temperature

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
 

Performed by

National Jewish

Performed

Tue-Sat

Methodology

Cell Culture

Reported

14 days

Synonyms

  • LYMPHOCYTE PROLIFERATION ASSAY
  • LAB3647
  • Antigen & Mitogen Proliferation Panel
  • Antigen & Mitogen Proliferation Panel
  • Blastogenesis Mitogens
  • Mitogen Studies
  • Lymphocyte Blastogenesis
  • Lymphocyte Blastogenesis Antigens
  • Blastogenesis Antigens

Reference Interval

By report

Performed by

National Jewish

CPT Codes

86353

Collection

LAB3647

Collect

10 ml Green (sodium heparin) - specimens must be collected within 24 hours of test performance. SAMPLES CAN BE SENT MON-THURS AND FRIDAY UNTIL NOON.

Specimen Preparation

Transport 30 mL whole blood  in original collection tubes. (Min: 10 mL)

Do not centrifuge, refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
 

Pediatric Collection

Infant Minimum: 10mL

Unacceptable Conditions

Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 24 hours.

Storage/Transport Temperature

Critical Room Temperature

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
 

Performed by

National Jewish

Ordering

Performed

Tue-Sat

Methodology

Cell Culture

Reported

14 days

Synonyms

  • LYMPHOCYTE PROLIFERATION ASSAY
  • LAB3647
  • Antigen & Mitogen Proliferation Panel
  • Antigen & Mitogen Proliferation Panel
  • Blastogenesis Mitogens
  • Mitogen Studies
  • Lymphocyte Blastogenesis
  • Lymphocyte Blastogenesis Antigens
  • Blastogenesis Antigens
Result Interpretation

Reference Interval

By report

Performed by

National Jewish

Administrative

CPT Codes

86353

LYMPHOCYTIC CHORIOMENING AB

LAB1251

 

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min 0.2 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Performed

Tue, Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-5 days

Synonyms

  • LCM Antibody
  • LAB1251
  • LCM S
  • LCM AB

Ordering Recommendations

Aid in the diagnosis of lymphocytic choriomeningitis (LCM) viral infection.

Reference Interval

Components
Reference Interval
Lymphocytic Choriomeningitis (LCM) Virus Antibody, IgG< 1:10 Negative - No significant level of LCM virus IgG antibody detected.
≥ 1:10 Positive - Presence of IgG antibody to LCM virus detected, suggestive of current or past infection.
Lymphocytic Choriomeningitis (LCM) Virus Antibody, IgM< 1:10 Negative - No significant level of LCM virus IgM antibody detected.
≥ 1:10 Positive - Presence of IgM antibody to LCM virus detected, suggestive of current or past infection.

Interpretive Data

The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

CPT Codes

86727 x2
Collection

LAB1251

 

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min 0.2 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Ordering

Performed

Tue, Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-5 days

Synonyms

  • LCM Antibody
  • LAB1251
  • LCM S
  • LCM AB

Ordering Recommendations

Aid in the diagnosis of lymphocytic choriomeningitis (LCM) viral infection.
Result Interpretation

Reference Interval

Components
Reference Interval
Lymphocytic Choriomeningitis (LCM) Virus Antibody, IgG< 1:10 Negative - No significant level of LCM virus IgG antibody detected.
≥ 1:10 Positive - Presence of IgG antibody to LCM virus detected, suggestive of current or past infection.
Lymphocytic Choriomeningitis (LCM) Virus Antibody, IgM< 1:10 Negative - No significant level of LCM virus IgM antibody detected.
≥ 1:10 Positive - Presence of IgM antibody to LCM virus detected, suggestive of current or past infection.

Interpretive Data

The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.

Administrative

CPT Codes

86727 x2