LAB3165
Collect
Serum separator tube.
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.
Unacceptable Conditions
Contaminated, hemolyzed, or severely lipemic specimens.
Storage/Transport Temperature
Refrigerated.
Stability (from collection to initiation)
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Notes
For follow-up testing on equivocal or positive results, refer to Legionella pneumophila Antibody (Type 1), IgG by IFA (0050376).
Performed
Mon-Fri
Methodology
Semi-Quantitative Indirect Fluorescent Antibody
Reported
1-4 days
Synonyms
- LAB3165
- LEG1-6 IGG
- LEGION1ABG
- LEGIONIGG
Ordering Recommendations
Provide retrospective evidence of suspected Legionella pneumophila infection.
Reference Interval
<1:128 Negative - No significant level of Legionella pneumophila Type 1-6 IgG antibody detected.
1:128 Equivocal - Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1:256 or greater Positive - Presence of Legionella pneumophila Type 1-6 IgG antibody detected, suggestive of current or past infection.
1:128 Equivocal - Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1:256 or greater Positive - Presence of Legionella pneumophila Type 1-6 IgG antibody detected, suggestive of current or past infection.
Interpretive Data
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. This assay may detect infection by any of the serotypes 1-6. The CDC and many state health laboratories recommend testing only for antibody to Legionella pneumophila Type 1. For equivocal or positive IFA results, the CDC protocol suggests follow-up testing for Legionella pneumophila antibody Type 1.
CPT Codes
86713
Collection |
LAB3165
Collect
Serum separator tube.
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.
Unacceptable Conditions
Contaminated, hemolyzed, or severely lipemic specimens.
Storage/Transport Temperature
Refrigerated.
Stability (from collection to initiation)
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Notes
For follow-up testing on equivocal or positive results, refer to Legionella pneumophila Antibody (Type 1), IgG by IFA (0050376).
Ordering |
Performed
Mon-Fri
Methodology
Semi-Quantitative Indirect Fluorescent Antibody
Reported
1-4 days
Synonyms
- LAB3165
- LEG1-6 IGG
- LEGION1ABG
- LEGIONIGG
Ordering Recommendations
Provide retrospective evidence of suspected Legionella pneumophila infection.
Result Interpretation |
Reference Interval
<1:128 Negative - No significant level of Legionella pneumophila Type 1-6 IgG antibody detected.
1:128 Equivocal - Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1:256 or greater Positive - Presence of Legionella pneumophila Type 1-6 IgG antibody detected, suggestive of current or past infection.
1:128 Equivocal - Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1:256 or greater Positive - Presence of Legionella pneumophila Type 1-6 IgG antibody detected, suggestive of current or past infection.
Interpretive Data
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. This assay may detect infection by any of the serotypes 1-6. The CDC and many state health laboratories recommend testing only for antibody to Legionella pneumophila Type 1. For equivocal or positive IFA results, the CDC protocol suggests follow-up testing for Legionella pneumophila antibody Type 1.
Administrative |
CPT Codes
86713