FACTOR VIII ACTIVITY w/HEPARINASE (HEPZYME)

LAB3058

F8 ACT HEP

Collect

One 2.7 mL blue top (3.2% citrate plasma).

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.5 mL platelet poor plasma.

Unacceptable Conditions

Clotted sample; hemolysed sample; tube fill volume less than 70% or greater than 110%; more than 4 hours since collection;  sample contaminated with I.V. fluid; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection, place at least 0.5 mL platelet poor plasma in an aliquot container and transport to the lab frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 4 hours.

Platelet poor plasma aliquot: Refrigerated, 4 hours; Frozen, (-20 degrees C), 2 weeks;  Frozen (-70 degrees C), 6 months.

Remarks

Samples containing small amounts of residual heparin are pre-treated with hepzyme prior to testing. Hepzyme neutralizes up to 2 U/mL of heparin..

Performed by

PCL Clinical Laboratory - Coagulation

Performed

Stats: daily, 24 hours/day; Routines: Daily, day shift only

Methodology

One stage clotting assay.

Reported

Same day

Synonyms

  • F8 ACT HEP
  • FACTOR 8 WITH HEPZYME

Reference Interval

51.0-186.0 U/dL

Performed by

PCL Clinical Laboratory - Coagulation

CPT Codes

85240

Collection

LAB3058

F8 ACT HEP

Collect

One 2.7 mL blue top (3.2% citrate plasma).

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.5 mL platelet poor plasma.

Unacceptable Conditions

Clotted sample; hemolysed sample; tube fill volume less than 70% or greater than 110%; more than 4 hours since collection;  sample contaminated with I.V. fluid; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection, place at least 0.5 mL platelet poor plasma in an aliquot container and transport to the lab frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 4 hours.

Platelet poor plasma aliquot: Refrigerated, 4 hours; Frozen, (-20 degrees C), 2 weeks;  Frozen (-70 degrees C), 6 months.

Remarks

Samples containing small amounts of residual heparin are pre-treated with hepzyme prior to testing. Hepzyme neutralizes up to 2 U/mL of heparin..

Performed by

PCL Clinical Laboratory - Coagulation

Ordering

Performed

Stats: daily, 24 hours/day; Routines: Daily, day shift only

Methodology

One stage clotting assay.

Reported

Same day

Synonyms

  • F8 ACT HEP
  • FACTOR 8 WITH HEPZYME
Result Interpretation

Reference Interval

51.0-186.0 U/dL

Performed by

PCL Clinical Laboratory - Coagulation

Administrative

CPT Codes

85240

FACTOR VIII INHIBITOR

LAB3059

F8 INHIB

Collect

Two 2.7 mL Blue tops (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 1 mL of platelet poor plasma.

Unacceptable Conditions

Clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; more than 4 hours since collection;  sample diluted with I.V.; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma from cells. Place plasma in aliquot container and transport to lab frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 4 hours.

Plasma aliquot: Refrigerated, 4 hours; Frozen (-20C), 2 weeks; Frozen (-70C), 6 months.

Remarks

Sample must be received by 11:00 AM for same day testing.

Reference range established using 3.2% sodium citrate. Patient history is helpful for interpretation of result.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Performed

Monday through Friday, day shift only

Methodology

Nijmegen-modified Bethesda assay

Reported

Same day

Synonyms

  • VIII INHIB
  • F8 INHIB

Reference Interval

0.0-0.4 U/mL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

CPT Codes

85335; and if indicated by medical director interpretation additional 85335-59

Collection

LAB3059

F8 INHIB

Collect

Two 2.7 mL Blue tops (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 1 mL of platelet poor plasma.

Unacceptable Conditions

Clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; more than 4 hours since collection;  sample diluted with I.V.; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma from cells. Place plasma in aliquot container and transport to lab frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 4 hours.

Plasma aliquot: Refrigerated, 4 hours; Frozen (-20C), 2 weeks; Frozen (-70C), 6 months.

Remarks

Sample must be received by 11:00 AM for same day testing.

Reference range established using 3.2% sodium citrate. Patient history is helpful for interpretation of result.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Ordering

Performed

Monday through Friday, day shift only

Methodology

Nijmegen-modified Bethesda assay

Reported

Same day

Synonyms

  • VIII INHIB
  • F8 INHIB
Result Interpretation

Reference Interval

0.0-0.4 U/mL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Administrative

CPT Codes

85335; and if indicated by medical director interpretation additional 85335-59

FACTOR VIII INHIBITOR W/HEPARINASE (HEPZYME)

LAB3568

F8 INHIB/HEP

 

Collect

Two 2.7 mL Blue Tops (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 1 mL of platelet poor plasma.

Unacceptable Conditions

Clotted sample; hemolyzed sample; tube fill volume less than 90% or greater than 110%; more than 4 hours since collection; sample contaminated with I.V. fluid; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma from cells. Place plasma in aliquot container and transport to lab frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 4 hours.

Platelet poor plasma: Refrigerated, 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70 degrees C), 6 months.

Remarks

Sample must be received by 11:00 AM for same day testing.

Samples containing small amount of residual heparin are pre-treated with hepzyme prior to testing. Patient history is helpful for interpretation of results.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Performed

Weekdays, day shift only

Methodology

Nijmegen-modified Bethesda assay

Reported

Same day

Synonyms

  • VIII IN HZ
  • Factor VIII Inhib with hepzyme
  • F8 INHIB/HEP

Reference Interval

0.0-0.4 U/mL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

CPT Codes

85335

Collection

LAB3568

F8 INHIB/HEP

 

Collect

Two 2.7 mL Blue Tops (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 1 mL of platelet poor plasma.

Unacceptable Conditions

Clotted sample; hemolyzed sample; tube fill volume less than 90% or greater than 110%; more than 4 hours since collection; sample contaminated with I.V. fluid; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma from cells. Place plasma in aliquot container and transport to lab frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 4 hours.

Platelet poor plasma: Refrigerated, 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70 degrees C), 6 months.

Remarks

Sample must be received by 11:00 AM for same day testing.

Samples containing small amount of residual heparin are pre-treated with hepzyme prior to testing. Patient history is helpful for interpretation of results.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Ordering

Performed

Weekdays, day shift only

Methodology

Nijmegen-modified Bethesda assay

Reported

Same day

Synonyms

  • VIII IN HZ
  • Factor VIII Inhib with hepzyme
  • F8 INHIB/HEP
Result Interpretation

Reference Interval

0.0-0.4 U/mL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Administrative

CPT Codes

85335

FACTOR X ACTIVITY (NOT ANTI-XA LEVEL)

LAB3060

F10

Collect

One 2.7 mL Blue top tube (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.5mL platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample contaminated with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma and store in plastic container. Transport plasma frozen.

Stability (from collection to initiation)

Whole blood room temp, 4 hours.

Platelet poor plasma: refrigerated (2-4C), 4 hours; frozen (-20C), 2 weeks; frozen (-70C), 6 months.

Remarks

Reference range established using 3.2% sodium citrate. Patient history is helpful for interpretation of result.

Performed by

PCL Clinical Laboratory - Coagulation

Performed

Stats: daily, 24 hours/day; Routines: Daily, day shift only

Methodology

Clotting assay

Reported

Same day

Synonyms

  • X ACT
  • FACTOR 10
  • F10

Reference Interval

Effective 01/11/2016:

69 - 145%

Before 01/11/2016:

65.0-131.0 U/dL

Performed by

PCL Clinical Laboratory - Coagulation

CPT Codes

85260

Collection

LAB3060

F10

Collect

One 2.7 mL Blue top tube (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.5mL platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample contaminated with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma and store in plastic container. Transport plasma frozen.

Stability (from collection to initiation)

Whole blood room temp, 4 hours.

Platelet poor plasma: refrigerated (2-4C), 4 hours; frozen (-20C), 2 weeks; frozen (-70C), 6 months.

Remarks

Reference range established using 3.2% sodium citrate. Patient history is helpful for interpretation of result.

Performed by

PCL Clinical Laboratory - Coagulation

Ordering

Performed

Stats: daily, 24 hours/day; Routines: Daily, day shift only

Methodology

Clotting assay

Reported

Same day

Synonyms

  • X ACT
  • FACTOR 10
  • F10
Result Interpretation

Reference Interval

Effective 01/11/2016:

69 - 145%

Before 01/11/2016:

65.0-131.0 U/dL

Performed by

PCL Clinical Laboratory - Coagulation

Administrative

CPT Codes

85260

FACTOR XI ACTIVITY

LAB309

F11

Collect

One 2.7 mL Blue top (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.5 mL of platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample diluted with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge the sample to obtain platelet poor plasma. Freeze plasma (use plastic only) and transport frozen.

Stability (from collection to initiation)

Whole blood room temp, 4 hours.

Platelet poor plasma: refrigerated (2-4C), 4 hours; frozen (-20C), 2 weeks; frozen (-70C), 6 months.

Remarks

Reference range established using 3.2% sodium citrate. Patient history is helpful for interpretation of result.

If sample is drawn from an indwelling catheter or if heparin contamination is suspected, order Factor XI Activity w/ Hepzyme (XI HEPZ) instead.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Performed

Stats: daily, 24 hours/day; Routines: Daily, day shift only

Methodology

Clotting assay

Reported

Same day

Synonyms

  • F11
  • XI ACT
  • FACTOR 11

Reference Interval

Effective 01/11/2016:

66 - 145%

Before 01/11/2016:

51.0-130.0 U/dL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

CPT Codes

85270; and if indicated by medical director interpretation additional 85270-59

Collection

LAB309

F11

Collect

One 2.7 mL Blue top (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.5 mL of platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample diluted with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge the sample to obtain platelet poor plasma. Freeze plasma (use plastic only) and transport frozen.

Stability (from collection to initiation)

Whole blood room temp, 4 hours.

Platelet poor plasma: refrigerated (2-4C), 4 hours; frozen (-20C), 2 weeks; frozen (-70C), 6 months.

Remarks

Reference range established using 3.2% sodium citrate. Patient history is helpful for interpretation of result.

If sample is drawn from an indwelling catheter or if heparin contamination is suspected, order Factor XI Activity w/ Hepzyme (XI HEPZ) instead.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Ordering

Performed

Stats: daily, 24 hours/day; Routines: Daily, day shift only

Methodology

Clotting assay

Reported

Same day

Synonyms

  • F11
  • XI ACT
  • FACTOR 11
Result Interpretation

Reference Interval

Effective 01/11/2016:

66 - 145%

Before 01/11/2016:

51.0-130.0 U/dL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Administrative

CPT Codes

85270; and if indicated by medical director interpretation additional 85270-59

FACTOR XI ACTIVITY w/HEPARINASE (HEPZYME)

LAB3007

XI HEPZYME

Collect

One 2.7 mL Blue Top (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.5 mL of platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample contaiminated with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge sample to obtain platelet poor plasma. Freeze plasma (use plastic tubes only) and transport frozen.

Stability (from collection to initiation)

Whole blood: Ambient temperature, 4 hours.

Platelet poor plasma:  Refrigerated (2-8 degrees C), 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70 degrees C), 6 months.

Remarks

Samples containing small amounts of residual heparin are pre-treated with hepzyme prior to testing. Hepzyme neutralizes up to 2 u/mL of heparin.  Patient history is helpful for interpretation of results.

 

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Performed

Stats: daily, 24 hours/day; Routines: Daily, day shift only

Methodology

One stage clotting assay

Reported

Same day

Synonyms

  • FACTOR 11 WITH HEPZYME
  • XI HEPZYME

Reference Interval

51.0-130.0 U/dL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

CPT Codes

85270-59

Collection

LAB3007

XI HEPZYME

Collect

One 2.7 mL Blue Top (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.5 mL of platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample contaiminated with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge sample to obtain platelet poor plasma. Freeze plasma (use plastic tubes only) and transport frozen.

Stability (from collection to initiation)

Whole blood: Ambient temperature, 4 hours.

Platelet poor plasma:  Refrigerated (2-8 degrees C), 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70 degrees C), 6 months.

Remarks

Samples containing small amounts of residual heparin are pre-treated with hepzyme prior to testing. Hepzyme neutralizes up to 2 u/mL of heparin.  Patient history is helpful for interpretation of results.

 

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Ordering

Performed

Stats: daily, 24 hours/day; Routines: Daily, day shift only

Methodology

One stage clotting assay

Reported

Same day

Synonyms

  • FACTOR 11 WITH HEPZYME
  • XI HEPZYME
Result Interpretation

Reference Interval

51.0-130.0 U/dL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Administrative

CPT Codes

85270-59

FACTOR XII ACTIVITY

LAB310

FACTOR XII

Effective 7/14/15 - Testing will be performed by PCL Clinical Laboratory.

Collect

Lt. blue (sodium citrate). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation

Transfer 2 mL platelet-poor plasma to an ARUP Standard Transport Tube. (Min: 1 mL)

Unacceptable Conditions

Serum. EDTA plasma, clotted or hemolyzed specimens.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge the sample to obtain platelet poor plasma. Place plasma in aliquot container and transport to lab frozen.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: Unacceptable; Frozen: 2 weeks

Performed

Mon-Sat

Methodology

Electromagnetic Mechanical Clot Detection

Reported

Same day

Synonyms

  • LAB310
  • F12

Ordering Recommendations

Evaluate the cause of an isolated prolonged PTT in a patient who is not currently bleeding.

Reference Interval

58-166%

CPT Codes

85280
Collection

LAB310

FACTOR XII

Effective 7/14/15 - Testing will be performed by PCL Clinical Laboratory.

Collect

Lt. blue (sodium citrate). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation

Transfer 2 mL platelet-poor plasma to an ARUP Standard Transport Tube. (Min: 1 mL)

Unacceptable Conditions

Serum. EDTA plasma, clotted or hemolyzed specimens.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge the sample to obtain platelet poor plasma. Place plasma in aliquot container and transport to lab frozen.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: Unacceptable; Frozen: 2 weeks
Ordering

Performed

Mon-Sat

Methodology

Electromagnetic Mechanical Clot Detection

Reported

Same day

Synonyms

  • LAB310
  • F12

Ordering Recommendations

Evaluate the cause of an isolated prolonged PTT in a patient who is not currently bleeding.
Result Interpretation

Reference Interval

58-166%
Administrative

CPT Codes

85280

FACTOR XIII QUALITATIVE

LAB3540

F13 QUAL

 

Collect

One 2.7 mL Blue top (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.3 mL of platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection;  clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample diluted with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge the sample to obtain platelet poor plasma. Freeze plasma (use plastic only) and transport frozen.

Stability (from collection to initiation)

Whole blood: Ambient temperature, 4 hours.

Platelet poor plasma: Refrigerated (2-4 degrees C), 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70 degrees C), 6 months.

Remarks

Reference range established using 3.2% sodium citrate. Factor XIII (fibrin stabilizing factor) forms stabilizing covalent bonds within fibrin stands and is necessary for stable clot formation.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Performed

Monday through Friday, dayshift

Methodology

Qualitative measurement of clot lysis. Abnormal result indicates <2% Factor XIII present.

Reported

Next day

Synonyms

  • F13 QUAL

Reference Interval

NORMAL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

CPT Codes

85291

Collection

LAB3540

F13 QUAL

 

Collect

One 2.7 mL Blue top (sodium citrate 3.2%). 

All blue top tubes collected for coagulation assays that do not have a blood culture collected first must have a discard tube collected prior to filling. The discard tube should be another blue top tube in which greater than 1 mL of blood is collected.

Pediatric Collection

Minimum volume: 0.3 mL of platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection;  clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample diluted with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge the sample to obtain platelet poor plasma. Freeze plasma (use plastic only) and transport frozen.

Stability (from collection to initiation)

Whole blood: Ambient temperature, 4 hours.

Platelet poor plasma: Refrigerated (2-4 degrees C), 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70 degrees C), 6 months.

Remarks

Reference range established using 3.2% sodium citrate. Factor XIII (fibrin stabilizing factor) forms stabilizing covalent bonds within fibrin stands and is necessary for stable clot formation.

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Ordering

Performed

Monday through Friday, dayshift

Methodology

Qualitative measurement of clot lysis. Abnormal result indicates <2% Factor XIII present.

Reported

Next day

Synonyms

  • F13 QUAL
Result Interpretation

Reference Interval

NORMAL

Performed by

PCL Clinical Laboratory - Specials (Coagulation)

Administrative

CPT Codes

85291

FATTY ACID PROFILE [C22-C26]

LAB3481

Collect

Green (sodium or lithium heparin) or lavender (EDTA).

Patient Preparation

Adults: Fasting specimen preferred.
Infants and children: Draw specimen prior to feeding or 2-3 hours after a meal.

Specimen Preparation

Separate plasma from cells. Transfer 0.5 mL plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.2 mL)

Unacceptable Conditions

Room temperature specimens greater than 24 hours. Refrigerated specimens greater than 48 hours. Specimens exposed to more than one freeze/thaw cycle.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: 1 mL serum or EDTA plasma

 

Spin and separate and deliver FROZEN to lab.

Stability (from collection to initiation)

After separation from cells: Ambient: 24 hours; Refrigerated: 72 hours; Frozen: 1 month

Remarks

Clinical information is needed for appropriate interpretation. Additional required information includes age, gender, diet (e.g.TPN therapy), drug therapy, and family history. Biochemical Genetics Patient History Form is available on the ARUP Web site at http://www.aruplab.com/patienthistory or by contacting ARUP Client Services.

Performed

Tue

Methodology

Liquid Chromatography-Tandem Mass Spectrometry

Reported

Varies

Synonyms

  • Very Long Chain Fatty Acid Profile
  • LAB3481
  • VLCFA

Ordering Recommendations

Initial test to screen for disorders of peroxisomal biogenesis and/or function, including X-linked adrenoleukodystrophy and Zellweger syndrome.

Reference Interval

Component
0-11 months
1 year to 2 years
3 years to 6 years
7 years and older
Pristanic AcidLess than 0.31 µmol/LLess than 0.55 µmol/LLess than 0.46 µmol/LLess than 0.26 µmol/L
Phytanic Acid0.03-2.13 µmol/L0.23-5.03 µmol/L0.33-2.53 µmol/L0.25- 2.07 µmol/L
Ratio Pristanic Acid to Phytanic AcidLess than 0.91Less than 0.28Less than 0.28Less than 0.28
C22:0 Behenic Acid28.94-93.50 µmol/L28.94-93.50 µmol/L28.94-93.50 µmol/L28.94-93.50 µmol/L
C24:0 Tetracosanoic Acid24.25-77.75 µmol/L24.25-77.75 µmol/L24.25-77.75 µmol/L24.25-77.75 µmol/L
C26:0 Hexacosanoic Acid0.17-0.73 µmol/L0.17-0.73 µmol/L0.17-0.73 µmol/L0.17-0.73 µmol/L
Ratio C24:0 to C22:00.64-1.020.64-1.020.64-1.020.64-1.02
Ratio C26:0 to C22:00.003-0.0150.003-0.0150.003-0.0150.003-0.015

Interpretive Data

Refer to report.

CPT Codes

82726
Collection

LAB3481

Collect

Green (sodium or lithium heparin) or lavender (EDTA).

Patient Preparation

Adults: Fasting specimen preferred.
Infants and children: Draw specimen prior to feeding or 2-3 hours after a meal.

Specimen Preparation

Separate plasma from cells. Transfer 0.5 mL plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.2 mL)

Unacceptable Conditions

Room temperature specimens greater than 24 hours. Refrigerated specimens greater than 48 hours. Specimens exposed to more than one freeze/thaw cycle.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: 1 mL serum or EDTA plasma

 

Spin and separate and deliver FROZEN to lab.

Stability (from collection to initiation)

After separation from cells: Ambient: 24 hours; Refrigerated: 72 hours; Frozen: 1 month

Remarks

Clinical information is needed for appropriate interpretation. Additional required information includes age, gender, diet (e.g.TPN therapy), drug therapy, and family history. Biochemical Genetics Patient History Form is available on the ARUP Web site at http://www.aruplab.com/patienthistory or by contacting ARUP Client Services.
Ordering

Performed

Tue

Methodology

Liquid Chromatography-Tandem Mass Spectrometry

Reported

Varies

Synonyms

  • Very Long Chain Fatty Acid Profile
  • LAB3481
  • VLCFA

Ordering Recommendations

Initial test to screen for disorders of peroxisomal biogenesis and/or function, including X-linked adrenoleukodystrophy and Zellweger syndrome.
Result Interpretation

Reference Interval

Component
0-11 months
1 year to 2 years
3 years to 6 years
7 years and older
Pristanic AcidLess than 0.31 µmol/LLess than 0.55 µmol/LLess than 0.46 µmol/LLess than 0.26 µmol/L
Phytanic Acid0.03-2.13 µmol/L0.23-5.03 µmol/L0.33-2.53 µmol/L0.25- 2.07 µmol/L
Ratio Pristanic Acid to Phytanic AcidLess than 0.91Less than 0.28Less than 0.28Less than 0.28
C22:0 Behenic Acid28.94-93.50 µmol/L28.94-93.50 µmol/L28.94-93.50 µmol/L28.94-93.50 µmol/L
C24:0 Tetracosanoic Acid24.25-77.75 µmol/L24.25-77.75 µmol/L24.25-77.75 µmol/L24.25-77.75 µmol/L
C26:0 Hexacosanoic Acid0.17-0.73 µmol/L0.17-0.73 µmol/L0.17-0.73 µmol/L0.17-0.73 µmol/L
Ratio C24:0 to C22:00.64-1.020.64-1.020.64-1.020.64-1.02
Ratio C26:0 to C22:00.003-0.0150.003-0.0150.003-0.0150.003-0.015

Interpretive Data

Refer to report.

Administrative

CPT Codes

82726

FATTY ACIDS, FREE

LAB986

Collect

Serum Separator Tube (SST). Collect on ice.

Patient Preparation

Overnight fasting specimen is preferred.

Specimen Preparation

Allow serum specimen to clot completely on ice. Serum must be separated from cells and frozen immediately, otherwise lipase continues to break down triglycerides, giving rise to elevated levels of nonesterified (free) fatty acids. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Unacceptable Conditions

Specimens collected in EDTA, heparin, sodium fluoride/potassium oxalate, sodium citrate, or ammonium oxalate. Non-frozen specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 1 month

Performed

Mon, Wed, Fri

Methodology

Quantitative Spectrophotometry

Reported

1-4 days

Synonyms

  • Free Fatty Acids
  • LAB986

Reference Interval

Effective November 17, 2014
0-5 months: less than or equal to 0.73 mmol/L
6 months-1 year: less than or equal to 0.99 mmol/L
2-17 years:  less than or equal to 1.78 mmol/L
18 years or older:  less than or equal to 0.78 mmol/L

CPT Codes

82725
Collection

LAB986

Collect

Serum Separator Tube (SST). Collect on ice.

Patient Preparation

Overnight fasting specimen is preferred.

Specimen Preparation

Allow serum specimen to clot completely on ice. Serum must be separated from cells and frozen immediately, otherwise lipase continues to break down triglycerides, giving rise to elevated levels of nonesterified (free) fatty acids. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Unacceptable Conditions

Specimens collected in EDTA, heparin, sodium fluoride/potassium oxalate, sodium citrate, or ammonium oxalate. Non-frozen specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 1 month
Ordering

Performed

Mon, Wed, Fri

Methodology

Quantitative Spectrophotometry

Reported

1-4 days

Synonyms

  • Free Fatty Acids
  • LAB986
Result Interpretation

Reference Interval

Effective November 17, 2014
0-5 months: less than or equal to 0.73 mmol/L
6 months-1 year: less than or equal to 0.99 mmol/L
2-17 years:  less than or equal to 1.78 mmol/L
18 years or older:  less than or equal to 0.78 mmol/L
Administrative

CPT Codes

82725

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