BONE MARROW TRANSPLANT FACT PANEL

LAB3474

Collect

1 - 6.0 mL Pink top

1 - 6.0 mL Purple Top

1 - 6.0 mL Red top with no gel

1 - 6.0 mL Gold with gel

Specimen Preparation

This test should ONLY be ordered on PCL patients (recipients and donors) that are preparing for a bone marrow transplant.

Performed by

Creative Test Solutions via

Bonfils Blood Center

Synonyms

  • LAB3474

Performed by

Creative Test Solutions via

Bonfils Blood Center

Collection

LAB3474

Collect

1 - 6.0 mL Pink top

1 - 6.0 mL Purple Top

1 - 6.0 mL Red top with no gel

1 - 6.0 mL Gold with gel

Specimen Preparation

This test should ONLY be ordered on PCL patients (recipients and donors) that are preparing for a bone marrow transplant.

Performed by

Creative Test Solutions via

Bonfils Blood Center

Ordering

Synonyms

  • LAB3474
Result Interpretation

Performed by

Creative Test Solutions via

Bonfils Blood Center

Administrative

BONE SPECIFIC ALKALINE PHOS

LAB1070

Collect

Serum separator tube. Also acceptable: Green (sodium or lithium heparin).

Specimen Preparation

Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Urine. Grossly hemolyzed specimens.

Storage/Transport Temperature

Frozen

Stability (from collection to initiation)

After separation from cells: Ambient: 2 hours; Refrigerated: 48 hours; Frozen: 2 months

Performed

Sun-Sat

Methodology

Quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • Alkaline Phosphatase, Bone Specific
  • LAB1070
  • BSAP

Additional Technical Information

Reference Interval

Effective November 14, 2011
Age
Male
Female
6 months-2 years31.6- 122.6  µg/L33.4- 145.3 µg/L
3-6 years31.3-103.4 µg/L32.9-108.6 µg/L
7-9 years48.6-140.4 µg/L36.3-159.4 µg/L
10-12 years48.8-155.5 µg/L44.2-163.3 µg/L
13-15 years27.8-210.9 µg/L14.8-136.2 µg/L
16-17 years15.3-126.8 µg/L10.5-44.8 µg/L
18-24 years10.0- 28.8 µg/L
25 years and older6.5-20.1 µg/L
Premenopausal Female 4.5-16.9 µg/L
Postmenopausal Female 7.0-22.4 µg/L

Interpretive Data

Liver alkaline phosphatase can affect the measurement of bone specific alkaline phosphatase in this assay.  Each 100 U/L of liver alkaline phosphatase contributes an additional 2.5 to 5.8 µg/L to the bone specific alkaline phosphatase result.

CPT Codes

84080
Collection

LAB1070

Collect

Serum separator tube. Also acceptable: Green (sodium or lithium heparin).

Specimen Preparation

Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Urine. Grossly hemolyzed specimens.

Storage/Transport Temperature

Frozen

Stability (from collection to initiation)

After separation from cells: Ambient: 2 hours; Refrigerated: 48 hours; Frozen: 2 months
Ordering

Performed

Sun-Sat

Methodology

Quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • Alkaline Phosphatase, Bone Specific
  • LAB1070
  • BSAP

Additional Technical Information

Result Interpretation

Reference Interval

Effective November 14, 2011
Age
Male
Female
6 months-2 years31.6- 122.6  µg/L33.4- 145.3 µg/L
3-6 years31.3-103.4 µg/L32.9-108.6 µg/L
7-9 years48.6-140.4 µg/L36.3-159.4 µg/L
10-12 years48.8-155.5 µg/L44.2-163.3 µg/L
13-15 years27.8-210.9 µg/L14.8-136.2 µg/L
16-17 years15.3-126.8 µg/L10.5-44.8 µg/L
18-24 years10.0- 28.8 µg/L
25 years and older6.5-20.1 µg/L
Premenopausal Female 4.5-16.9 µg/L
Postmenopausal Female 7.0-22.4 µg/L

Interpretive Data

Liver alkaline phosphatase can affect the measurement of bone specific alkaline phosphatase in this assay.  Each 100 U/L of liver alkaline phosphatase contributes an additional 2.5 to 5.8 µg/L to the bone specific alkaline phosphatase result.

Administrative

CPT Codes

84080

Borrelia burgdoferi Antibodies, Total w/reflex to IgG and IgM by Western Blot

LAB3222

 

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Unacceptable Conditions

CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

This panel is appropriate for Lyme disease testing less than 4 weeks from erythema migrans or onset of disease symptoms.

A negative result indicates the immunoblot evaluation for B. burgdorferi antibody demonstrates no antibodies unique to B. burgdorferi and is, therefore, not supportive of Lyme disease.

A positive result indicates that the immunoblot evaluation for B. burgdorferi antibody is consistent with the presence of antibody produced by patients in response to infection by B. burgdorferi and suggests the presence of Lyme disease. Although the test has been shown to have a high degree of reliability for diagnostic purposes, laboratory data should always be correlated with clinical findings.

Current CDC recommendations for the serological diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocals and positives with immunoblot. Both IgM and IgG immunoblots should be performed on specimens obtained less than 4 weeks after appearance of erythema migrans. Only IgG immunoblot is to be performed on specimens greater than 4 weeks after disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease.

If ELISA result is 1.00 LIV or greater, then IgG and IgM immunoblot will be added. Additional charges apply.

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Reported

1-3 days

Synonyms

  • LAB3222
  • LYME ACUTE

Ordering Recommendations

Preferred reflex test to detect Lyme disease in individuals with ≤4 weeks of clinical symptoms or exposure to tick. Positive/equivocal screen confirmed by immunoblot.

Reference Interval

Components
Reference Interval
Borrelia burgdorferi Antibodies, Total by ELISA
0.99 LIV or lessNegative - Antibody to B. burgdorferi not detected.
1.00-1.20 LIVEquivocal - Repeat testing in 10-14 days may be helpful.
1.21 LIV or greaterPositive - Probable presence of antibody to B. burgdorferi detected.

Borrelia burgdorferi Antibody, IgG by ImmunoblotEffective August 15, 2011

Negative
Borrelia burgdorferi Antibody, IgM by ImmunoblotEffective August 15, 2011

Negative

Interpretive Data

Refer to report.

CPT Codes

86618; if reflexed, add 86617 x2
Collection

LAB3222

 

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Unacceptable Conditions

CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

This panel is appropriate for Lyme disease testing less than 4 weeks from erythema migrans or onset of disease symptoms.

A negative result indicates the immunoblot evaluation for B. burgdorferi antibody demonstrates no antibodies unique to B. burgdorferi and is, therefore, not supportive of Lyme disease.

A positive result indicates that the immunoblot evaluation for B. burgdorferi antibody is consistent with the presence of antibody produced by patients in response to infection by B. burgdorferi and suggests the presence of Lyme disease. Although the test has been shown to have a high degree of reliability for diagnostic purposes, laboratory data should always be correlated with clinical findings.

Current CDC recommendations for the serological diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocals and positives with immunoblot. Both IgM and IgG immunoblots should be performed on specimens obtained less than 4 weeks after appearance of erythema migrans. Only IgG immunoblot is to be performed on specimens greater than 4 weeks after disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease.

If ELISA result is 1.00 LIV or greater, then IgG and IgM immunoblot will be added. Additional charges apply.
Ordering

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Reported

1-3 days

Synonyms

  • LAB3222
  • LYME ACUTE

Ordering Recommendations

Preferred reflex test to detect Lyme disease in individuals with ≤4 weeks of clinical symptoms or exposure to tick. Positive/equivocal screen confirmed by immunoblot.
Result Interpretation

Reference Interval

Components
Reference Interval
Borrelia burgdorferi Antibodies, Total by ELISA
0.99 LIV or lessNegative - Antibody to B. burgdorferi not detected.
1.00-1.20 LIVEquivocal - Repeat testing in 10-14 days may be helpful.
1.21 LIV or greaterPositive - Probable presence of antibody to B. burgdorferi detected.

Borrelia burgdorferi Antibody, IgG by ImmunoblotEffective August 15, 2011

Negative
Borrelia burgdorferi Antibody, IgM by ImmunoblotEffective August 15, 2011

Negative

Interpretive Data

Refer to report.

Administrative

CPT Codes

86618; if reflexed, add 86617 x2

BORRELIA BURGDORFERI BY PCR

LAB3711

Collect

Lavender (EDTA), pink (K2EDTA) or serum separator tube. OR CSF, synovial fluid or tissue.

Specimen Preparation

Separate serum or plasma from cells. Transfer 1 mL serum, plasma, CSF or synovial fluid to a sterile container. (Min: 0.5 mL). Tissue: Transfer to a sterile container and freeze immediately.

Unacceptable Conditions

Heparinized specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 year
All Others: Ambient: 8 hours; Refrigerated: 72 hours; Frozen: 1 year

Remarks

Specimen source required.

Performed

Mon, Wed, Fri

Methodology

Qualitative Polymerase Chain Reaction

Reported

1-4 days

Synonyms

  • Lyme Disease by PCR
  • LAB3711
  • LYMEPCR

Ordering Recommendations

Not a first-line test for Lyme disease. May be useful if strong suspicion of Lyme disease persists in spite of persistent negative serologic testing.

CPT Codes

87476
Collection

LAB3711

Collect

Lavender (EDTA), pink (K2EDTA) or serum separator tube. OR CSF, synovial fluid or tissue.

Specimen Preparation

Separate serum or plasma from cells. Transfer 1 mL serum, plasma, CSF or synovial fluid to a sterile container. (Min: 0.5 mL). Tissue: Transfer to a sterile container and freeze immediately.

Unacceptable Conditions

Heparinized specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 year
All Others: Ambient: 8 hours; Refrigerated: 72 hours; Frozen: 1 year

Remarks

Specimen source required.
Ordering

Performed

Mon, Wed, Fri

Methodology

Qualitative Polymerase Chain Reaction

Reported

1-4 days

Synonyms

  • Lyme Disease by PCR
  • LAB3711
  • LYMEPCR

Ordering Recommendations

Not a first-line test for Lyme disease. May be useful if strong suspicion of Lyme disease persists in spite of persistent negative serologic testing.
Result Interpretation
Administrative

CPT Codes

87476

BORRELIA HERMSII AB - IGG/IGM

LAB1205

Collect

Plain red or serum separator tube (SST).

Specimen Preparation

Transfer 1 mL serum to an ARUP Standard Transport Tube (Min: 0.25 mL)

Storage/Transport Temperature

Refrigerated. Also acceptable: Frozen.

Stability (from collection to initiation)

Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1 month

Performed

Varies

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

3-11 days

Synonyms

  • LAB1205

Ordering Recommendations

Use for diagnostic workup of suspected tick-borne relapsing fever.

Reference Interval

By report

CPT Codes

86619 x2
Collection

LAB1205

Collect

Plain red or serum separator tube (SST).

Specimen Preparation

Transfer 1 mL serum to an ARUP Standard Transport Tube (Min: 0.25 mL)

Storage/Transport Temperature

Refrigerated. Also acceptable: Frozen.

Stability (from collection to initiation)

Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1 month
Ordering

Performed

Varies

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

3-11 days

Synonyms

  • LAB1205

Ordering Recommendations

Use for diagnostic workup of suspected tick-borne relapsing fever.
Result Interpretation

Reference Interval

By report
Administrative

CPT Codes

86619 x2

BRUCELLA ANTIBODY (TOTAL)

LAB221

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 6 months (avoid repeated freeze/thaw cycles)

Performed

Mon-Fri

Methodology

Semi-Quantitative Agglutination

Reported

2-4 days

Synonyms

  • LAB221
  • BRUC

Ordering Recommendations

Recommended serology test to detect recent infection from Brucella in the context of a clinically compatible illness and exposure history.

Reference Interval

 < 1:20 Negative

Interpretive Data

Cross-reactions may occur between Brucella and F. tularensis antigens and antisera; therefore, parallel tests should be run with these antigens. A fourfold rise in titer is considered diagnostic. A single serum titer of 1:80 or 1:160 is suggestive of brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures.

CPT Codes

86622
Collection

LAB221

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 6 months (avoid repeated freeze/thaw cycles)
Ordering

Performed

Mon-Fri

Methodology

Semi-Quantitative Agglutination

Reported

2-4 days

Synonyms

  • LAB221
  • BRUC

Ordering Recommendations

Recommended serology test to detect recent infection from Brucella in the context of a clinically compatible illness and exposure history.
Result Interpretation

Reference Interval

 < 1:20 Negative

Interpretive Data

Cross-reactions may occur between Brucella and F. tularensis antigens and antisera; therefore, parallel tests should be run with these antigens. A fourfold rise in titer is considered diagnostic. A single serum titer of 1:80 or 1:160 is suggestive of brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures.

Administrative

CPT Codes

86622

BUFFY COAT SMEAR-MICROBIOLOGY

Collect

5 mL purple top [EDTA] top tube.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: 1-3 mL purple top [EDTA] top tube. Refrigerated and transport immediately.

Stability (from collection to initiation)

Room Temp: 1 hour; Refrigerated: ASAP.

Remarks

Consult with the Infectious Disease Service. Specify suspected organism. Submit promptly to the laboratory for processing.

Performed by

PCL Clinical Laboratory

Performed

Daily

Methodology

Gram Stained smears of buffy coat to look for fungal elements or intracellular bacteria.

Reported

Within 24 hours

Performed by

PCL Clinical Laboratory

CPT Codes

87205
Collection

Collect

5 mL purple top [EDTA] top tube.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: 1-3 mL purple top [EDTA] top tube. Refrigerated and transport immediately.

Stability (from collection to initiation)

Room Temp: 1 hour; Refrigerated: ASAP.

Remarks

Consult with the Infectious Disease Service. Specify suspected organism. Submit promptly to the laboratory for processing.

Performed by

PCL Clinical Laboratory
Ordering

Performed

Daily

Methodology

Gram Stained smears of buffy coat to look for fungal elements or intracellular bacteria.

Reported

Within 24 hours
Result Interpretation

Performed by

PCL Clinical Laboratory
Administrative

CPT Codes

87205

BUPIVACAINE

LAB3094

Collect

3 mL serum from Plain Red Top tube EDTA or Heparinized plasma [not from PST tube]

Unacceptable Conditions

Samples submitted in SST or PST tubes.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 1.5 mL serum/plasma Ship to lab at ambient temp.

Stability (from collection to initiation)

.

Remarks

Do not use serum or plasma seprator tubes.

Performed by

National Medical Services

Performed

Varies

Methodology

Gas Chromatography

Reported

.

Synonyms

  • LAB3094

Performed by

National Medical Services

CPT Codes

82491

Collection

LAB3094

Collect

3 mL serum from Plain Red Top tube EDTA or Heparinized plasma [not from PST tube]

Unacceptable Conditions

Samples submitted in SST or PST tubes.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 1.5 mL serum/plasma Ship to lab at ambient temp.

Stability (from collection to initiation)

.

Remarks

Do not use serum or plasma seprator tubes.

Performed by

National Medical Services

Ordering

Performed

Varies

Methodology

Gas Chromatography

Reported

.

Synonyms

  • LAB3094
Result Interpretation

Performed by

National Medical Services

Administrative

CPT Codes

82491