BETA-2 TRANSFERRIN

LAB205

Collect

Aural or nasal fluid in sterile container.

Specimen Preparation

Do not freeze.   Transport 2 mL aural or nasal fluid in a tube without preservative. (Min: 1 mL aural or nasal fluid)

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Notes

The beta-2 transferrin protein assay by IFE methodology is not a reliable method for detecting human perilymph due to the low sensitivity of the assay.

Performed

Sun-Sat

Methodology

Qualitative Immunofixation Electrophoresis

Reported

1-4 days

Synonyms

  • Transferrin, Beta-2
  • LAB205
  • B2TRNSF

Ordering Recommendations

Aids in diagnosis of CSF leak.

Reference Interval

None detected

Interpretive Data

Detection of a beta-2 transferrin band by IFE is diagnostic for the presence of cerebrospinal fluid (CSF). This test is useful in the differential diagnosis for CSF otorrhea or CSF rhinorrhea. Beta-2 transferrin is not detected by this methodology in normal serum, tears, saliva, sputum, nasal, or aural fluid.

The performance characteristics of this test were determined by PCL Clinical Laboratory, Inc.

CPT Codes

86335
Collection

LAB205

Collect

Aural or nasal fluid in sterile container.

Specimen Preparation

Do not freeze.   Transport 2 mL aural or nasal fluid in a tube without preservative. (Min: 1 mL aural or nasal fluid)

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable

Notes

The beta-2 transferrin protein assay by IFE methodology is not a reliable method for detecting human perilymph due to the low sensitivity of the assay.
Ordering

Performed

Sun-Sat

Methodology

Qualitative Immunofixation Electrophoresis

Reported

1-4 days

Synonyms

  • Transferrin, Beta-2
  • LAB205
  • B2TRNSF

Ordering Recommendations

Aids in diagnosis of CSF leak.
Result Interpretation

Reference Interval

None detected

Interpretive Data

Detection of a beta-2 transferrin band by IFE is diagnostic for the presence of cerebrospinal fluid (CSF). This test is useful in the differential diagnosis for CSF otorrhea or CSF rhinorrhea. Beta-2 transferrin is not detected by this methodology in normal serum, tears, saliva, sputum, nasal, or aural fluid.

The performance characteristics of this test were determined by PCL Clinical Laboratory, Inc.

Administrative

CPT Codes

86335

BETA-2-GLYCOPROTEIN 1 IGA AB

LAB3116

Collect

One 5 mL gold or red top with gel.

Pediatric Collection

0.15 mL serum at 2-8°C.

Unacceptable Conditions

Excessuve hemolysis, chylous serum, gross contamination, inadequate volume of blood, inadequate patient and/or specimen identification.

Storage/Transport Temperature

Internal:Deliver to lab immediately.

Offsite: Separate serum from cells ASAP. Transfer 1.0 mL into aliquot tube, deliver to lab refrigerated.

Stability (from collection to initiation)

After separation from cells: Refrigerated: 1-2 weeks; Frozen: Indefinitely

Remarks

Remove serum from clot within 2 hours after drawing. Refrigerate serum.

Performed by

PCL Clinical Laboratory

Performed

Once weekly

Methodology

Enzyme-Linked Immunoassay

Reported

In 14 days

Synonyms

  • LAB3116

Reference Interval

Negative

Performed by

PCL Clinical Laboratory

CPT Codes

86146

Collection

LAB3116

Collect

One 5 mL gold or red top with gel.

Pediatric Collection

0.15 mL serum at 2-8°C.

Unacceptable Conditions

Excessuve hemolysis, chylous serum, gross contamination, inadequate volume of blood, inadequate patient and/or specimen identification.

Storage/Transport Temperature

Internal:Deliver to lab immediately.

Offsite: Separate serum from cells ASAP. Transfer 1.0 mL into aliquot tube, deliver to lab refrigerated.

Stability (from collection to initiation)

After separation from cells: Refrigerated: 1-2 weeks; Frozen: Indefinitely

Remarks

Remove serum from clot within 2 hours after drawing. Refrigerate serum.

Performed by

PCL Clinical Laboratory

Ordering

Performed

Once weekly

Methodology

Enzyme-Linked Immunoassay

Reported

In 14 days

Synonyms

  • LAB3116
Result Interpretation

Reference Interval

Negative

Performed by

PCL Clinical Laboratory

Administrative

CPT Codes

86146

BETA-2-GLYCOPROTEIN 1 IGG/IGM

LAB1179

Collect

One 7 mL red top with gel

Unacceptable Conditions

Excessive hemolysis, chylous serum, gross contamination, inadequate volume of blood, inadequate patient and/or specimen identification.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: Separate serum from cells ASAP. Transfer 2 mL into aliquot tube, deliver to lab refrigerated.

Stability (from collection to initiation)

Refrigerated: 1-2 weeks; Frozen: Indefinitely.

Remarks

Remove serum from clot wthin 2 hours after drawing. Refrigerate specimen.

Performed by

PCL Clinical Laboratory

Performed

Once Weekly

Methodology

Enzyme-linked immunoassay

Reported

In 14 days

Synonyms

  • -716 Beta-2-Glycoprotein Antibodies IgG/IgM
  • LAB1179
  • B2G IGG/IGM

Reference Interval

Beta-2-Glycoprotein 1 IgG: Negative

Beta-2-Glycoprotein 1 IgM: Negative

Performed by

PCL Clinical Laboratory

CPT Codes

86146 (2)

Collection

LAB1179

Collect

One 7 mL red top with gel

Unacceptable Conditions

Excessive hemolysis, chylous serum, gross contamination, inadequate volume of blood, inadequate patient and/or specimen identification.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: Separate serum from cells ASAP. Transfer 2 mL into aliquot tube, deliver to lab refrigerated.

Stability (from collection to initiation)

Refrigerated: 1-2 weeks; Frozen: Indefinitely.

Remarks

Remove serum from clot wthin 2 hours after drawing. Refrigerate specimen.

Performed by

PCL Clinical Laboratory

Ordering

Performed

Once Weekly

Methodology

Enzyme-linked immunoassay

Reported

In 14 days

Synonyms

  • -716 Beta-2-Glycoprotein Antibodies IgG/IgM
  • LAB1179
  • B2G IGG/IGM
Result Interpretation

Reference Interval

Beta-2-Glycoprotein 1 IgG: Negative

Beta-2-Glycoprotein 1 IgM: Negative

Performed by

PCL Clinical Laboratory

Administrative

CPT Codes

86146 (2)

BETA-2-MICROBGLOBULIN, CSF

LAB181

Collect

CSF. Also acceptable: CSF collected in plain red or green (sodium or lithium heparin).

Specimen Preparation

Centrifuge to remove cellular material. Transfer 0.5 mL CSF to an ARUP Standard Transport Tube. (Min: 0.4 mL)

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cellular material: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 2 weeks

Performed

Sun-Sat

Methodology

Quantitative Immunoturbidimetry

Reported

Within 24 hours

Synonyms

  • B2M CSF
  • LAB181

Reference Interval

0.0-2.4 mg/L

CPT Codes

82232
Collection

LAB181

Collect

CSF. Also acceptable: CSF collected in plain red or green (sodium or lithium heparin).

Specimen Preparation

Centrifuge to remove cellular material. Transfer 0.5 mL CSF to an ARUP Standard Transport Tube. (Min: 0.4 mL)

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cellular material: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 2 weeks
Ordering

Performed

Sun-Sat

Methodology

Quantitative Immunoturbidimetry

Reported

Within 24 hours

Synonyms

  • B2M CSF
  • LAB181
Result Interpretation

Reference Interval

0.0-2.4 mg/L
Administrative

CPT Codes

82232

BETA-2-MICROGLOBULIN SERUM

LAB49

B2M

Collect

One 5.0 mL gold or red top with gel (SST), (clot activator serum).

Unacceptable Conditions

Gross lipemia; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal:  Allow to clot for 30 minutes, then deliver to lab immediately at ambient temperature.
Offsite: Allow to clot 30 minutes, immediately centrifuge, place at least 1 mL of serum in an aliquot container and transport to lab at 2-8 degrees C.

Stability (from collection to initiation)

Before centrifugation: Ambient, 1 hour.

After centrifugation: Ambient, 1 hour.

Serum aliquot: Ambient, 1 hour; Refrigerated, 8 days; Frozen (-20 degrees C), 2 months.

Remarks

Separate serum from cells as soon as possible and refrigerate.

Performed by

PCL Clinical Laboratory - Special Chemistry

Notes

Due to the half-life of the protein in the system, any Beta-2-Microglobulin order that is placed within 20 hours of a previous Beta-2-Microglobulin level will be considered a duplicate order and will be cancelled.

Performed

Monday and Thursday

Methodology

Nephelometry

Reported

Same day

Synonyms

  • LAB49
  • B2M

Reference Interval

1.3-2.9 mg/L

Performed by

PCL Clinical Laboratory - Special Chemistry

CPT Codes

82232

Collection

LAB49

B2M

Collect

One 5.0 mL gold or red top with gel (SST), (clot activator serum).

Unacceptable Conditions

Gross lipemia; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal:  Allow to clot for 30 minutes, then deliver to lab immediately at ambient temperature.
Offsite: Allow to clot 30 minutes, immediately centrifuge, place at least 1 mL of serum in an aliquot container and transport to lab at 2-8 degrees C.

Stability (from collection to initiation)

Before centrifugation: Ambient, 1 hour.

After centrifugation: Ambient, 1 hour.

Serum aliquot: Ambient, 1 hour; Refrigerated, 8 days; Frozen (-20 degrees C), 2 months.

Remarks

Separate serum from cells as soon as possible and refrigerate.

Performed by

PCL Clinical Laboratory - Special Chemistry

Notes

Due to the half-life of the protein in the system, any Beta-2-Microglobulin order that is placed within 20 hours of a previous Beta-2-Microglobulin level will be considered a duplicate order and will be cancelled.

Ordering

Performed

Monday and Thursday

Methodology

Nephelometry

Reported

Same day

Synonyms

  • LAB49
  • B2M
Result Interpretation

Reference Interval

1.3-2.9 mg/L

Performed by

PCL Clinical Laboratory - Special Chemistry

Administrative

CPT Codes

82232

BETA-2-MICROGLOBULIN URINE

LAB3306

Collect

Random urine.

Patient Preparation

Void the urinary bladder, then drink a large glass of water and collect a urine specimen within 1 hour.

Specimen Preparation

Transfer one 3 mL aliquot from a well-mixed random collection to an ARUP Standard Transport Tube. (Min: 1 mL)
If pH is greater than 8, lower pH to 6-8 by adding 1M HCL. If pH less than 6, increase pH to 6-8 by adding 5% NaOH. Titrate with appropriate preservative until pH of 6-8 has been reached. Record the pH on the transport tube and test request form.

Storage/Transport Temperature

Frozen

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 48 hours; Frozen: 2 months

Performed

Sun-Sat

Methodology

Quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • LAB3306
  • B2MU

Reference Interval

Effective February 18, 2014
Components
Reference Interval
Beta-2-Microglobulin, UrineEffective February 18, 2014
0-300 µg/L
Beta-2-Microglobulin, ratio to CRT0-300 µg/g CRT

CPT Codes

82232
Collection

LAB3306

Collect

Random urine.

Patient Preparation

Void the urinary bladder, then drink a large glass of water and collect a urine specimen within 1 hour.

Specimen Preparation

Transfer one 3 mL aliquot from a well-mixed random collection to an ARUP Standard Transport Tube. (Min: 1 mL)
If pH is greater than 8, lower pH to 6-8 by adding 1M HCL. If pH less than 6, increase pH to 6-8 by adding 5% NaOH. Titrate with appropriate preservative until pH of 6-8 has been reached. Record the pH on the transport tube and test request form.

Storage/Transport Temperature

Frozen

Stability (from collection to initiation)

Ambient: 8 hours; Refrigerated: 48 hours; Frozen: 2 months
Ordering

Performed

Sun-Sat

Methodology

Quantitative Chemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • LAB3306
  • B2MU
Result Interpretation

Reference Interval

Effective February 18, 2014
Components
Reference Interval
Beta-2-Microglobulin, UrineEffective February 18, 2014
0-300 µg/L
Beta-2-Microglobulin, ratio to CRT0-300 µg/g CRT

Administrative

CPT Codes

82232

BICARBONATE [HCO3], URINE

LAB3303

Collect

Random urine in sealed container.

Specimen Preparation

Immediately upon collection, mix and transfer 4 mL urine to an ARUP Standard Transport Tube. (Min: 0.3 mL) Do not expose to air.

Unacceptable Conditions

Room temperature or refrigerated specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month (unstable if thawed and refrozen)

Performed

Sun-Sat

Methodology

Enzymatic

Reported

Within 24 hours

Synonyms

  • LAB3303
  • BICARB

Reference Interval

Not defined.

Interpretive Data

Reference Interval has not been defined for Bicarbonate, Urine.

CPT Codes

82374
Collection

LAB3303

Collect

Random urine in sealed container.

Specimen Preparation

Immediately upon collection, mix and transfer 4 mL urine to an ARUP Standard Transport Tube. (Min: 0.3 mL) Do not expose to air.

Unacceptable Conditions

Room temperature or refrigerated specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month (unstable if thawed and refrozen)
Ordering

Performed

Sun-Sat

Methodology

Enzymatic

Reported

Within 24 hours

Synonyms

  • LAB3303
  • BICARB
Result Interpretation

Reference Interval

Not defined.

Interpretive Data

Reference Interval has not been defined for Bicarbonate, Urine.

Administrative

CPT Codes

82374

BILE ACIDS

LAB693

Collect

Serum separator tube.

Patient Preparation

Patient should fast for 8 hours prior to collection.

Specimen Preparation

Allow specimen to clot completely at room temperature before centrifugation. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Body fluids. Heparinized specimens. Hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 3 months

Performed

Sun-Sat

Methodology

Quantitative Enzymatic

Reported

Within 24 hours

Synonyms

  • LAB693

Ordering Recommendations

Use to detect hepatobiliary dysfunction. Do not order to detect inborn errors of bile acid metabolism. May aid in diagnosis of intrahepatic cholestasis of pregnancy.

Reference Interval

0-10 µmol/L

Interpretive Data

Reference interval applies to fasting specimens.

CPT Codes

82239
Collection

LAB693

Collect

Serum separator tube.

Patient Preparation

Patient should fast for 8 hours prior to collection.

Specimen Preparation

Allow specimen to clot completely at room temperature before centrifugation. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Body fluids. Heparinized specimens. Hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 3 months
Ordering

Performed

Sun-Sat

Methodology

Quantitative Enzymatic

Reported

Within 24 hours

Synonyms

  • LAB693

Ordering Recommendations

Use to detect hepatobiliary dysfunction. Do not order to detect inborn errors of bile acid metabolism. May aid in diagnosis of intrahepatic cholestasis of pregnancy.
Result Interpretation

Reference Interval

0-10 µmol/L

Interpretive Data

Reference interval applies to fasting specimens.

Administrative

CPT Codes

82239

BILIRUBIN DIRECT PANEL

LAB4413

BILI D PANEL

Collect

One 4.5 mL Green top with gel (PST), (lithium heparin plasma). Protect from light.

Pediatric Collection

At least 0.5 mL whole blood collected in a microtainer, green top, lithium heparin plasma.

Unacceptable Conditions

Lipemic sample; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection. Transport to laboratory at 2-8 degrees C.

Stability (from collection to initiation)

Before centrifugation: Ambient, 1 hour.

After centrifugation in gel tube: Ambient, 8 hours; Refrigerated, 2 days; Frozen, Unacceptable.

Plasma aliquot: Ambient, 8 hours; Refrigerated: 2 days; Frozen (-20 degrees C), Unknown. 

Frozen samples may be thawed one time only.

Remarks

Protect from light.

Performed by

PCL Clinical Laboratory - Core

Performed

Upon receipt

Methodology

Diazo

Reported

Same day

Synonyms

  • -105
  • LAB52

Reference Interval

Effective 05/20/2014:

Component Range
BILIRUBIN, TOTAL 0.1 - 1.3 mg/dL
BILIRUBIN, DIRECT 0.0 - 0.2 mg/dL
BILIRUBIN, INDIRECT 0 - 1 mg/dL

 

Before 05/20/2014:

Component Sex From Age To Age Normal Low Normal High Units
BILIRUBIN, TOTAL M/F <> 1M 0.0 12.0 mg/dL
  M/F 1M 150 0.0 1.3 mg/dL
BILIRUBIN, DIRECT M/F 0 150 0.0 0.4 mg/dL
BILIRUBIN, INDIRECT M/F 0 1M 0.0 11.7 mg/dL
  M/F 1M 150 0.0 0.9 mg/dL

Interpretive Data

Adult reference range.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

82247 Bili Total; 82248 Bili Direct

Collection

LAB4413

BILI D PANEL

Collect

One 4.5 mL Green top with gel (PST), (lithium heparin plasma). Protect from light.

Pediatric Collection

At least 0.5 mL whole blood collected in a microtainer, green top, lithium heparin plasma.

Unacceptable Conditions

Lipemic sample; sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection. Transport to laboratory at 2-8 degrees C.

Stability (from collection to initiation)

Before centrifugation: Ambient, 1 hour.

After centrifugation in gel tube: Ambient, 8 hours; Refrigerated, 2 days; Frozen, Unacceptable.

Plasma aliquot: Ambient, 8 hours; Refrigerated: 2 days; Frozen (-20 degrees C), Unknown. 

Frozen samples may be thawed one time only.

Remarks

Protect from light.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Upon receipt

Methodology

Diazo

Reported

Same day

Synonyms

  • -105
  • LAB52
Result Interpretation

Reference Interval

Effective 05/20/2014:

Component Range
BILIRUBIN, TOTAL 0.1 - 1.3 mg/dL
BILIRUBIN, DIRECT 0.0 - 0.2 mg/dL
BILIRUBIN, INDIRECT 0 - 1 mg/dL

 

Before 05/20/2014:

Component Sex From Age To Age Normal Low Normal High Units
BILIRUBIN, TOTAL M/F <> 1M 0.0 12.0 mg/dL
  M/F 1M 150 0.0 1.3 mg/dL
BILIRUBIN, DIRECT M/F 0 150 0.0 0.4 mg/dL
BILIRUBIN, INDIRECT M/F 0 1M 0.0 11.7 mg/dL
  M/F 1M 150 0.0 0.9 mg/dL

Interpretive Data

Adult reference range.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

82247 Bili Total; 82248 Bili Direct

BILIRUBIN TOTAL

LAB50

BILI T

Collect

One 4.5 mL Green top with gel (PST), (lithium heparin plasma).

Protect from light.

Pediatric Collection

At least 0.5 mL whole blood collected in a microtainer, green top with gel, lithium heparin plasma. Protect from light.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection.  Transport to laboratory at 2-8 degrees C.

Stability (from collection to initiation)

Before centrifugation: Ambient, 1 hour.

After centrifugation in gel tube: Ambient, 8 hours; Refrigerated, 2 days; Frozen, Unacceptable.

Plasma aliquot: Ambient, 8 hours; Refrigerated, 2 days; Frozen, Unknown.

Frozen samples may be thawed one time only.

Remarks

Protect from light. Naproxen can result in spurious elevations in Total Bilirubin Levels.

Performed by

PCL Clinical Laboratory - Core

Performed

Upon receipt

Methodology

Jendrassik-Grof

Reported

Same day

Synonyms

  • LAB50
  • BILI T

Reference Interval

Effective 05/20/2014:

0.1 - 1.3 mg/dL

Before 05/20/2014:

Sex From Age To Age Normal Low Normal High Units
M/F 0 1M 0.0 12.0 mg/dL
M/F 1M 150 0.0 1.3 mg/dL

Interpretive Data

Adult reference range.

Performed by

PCL Clinical Laboratory - Core

CPT Codes

82247

Collection

LAB50

BILI T

Collect

One 4.5 mL Green top with gel (PST), (lithium heparin plasma).

Protect from light.

Pediatric Collection

At least 0.5 mL whole blood collected in a microtainer, green top with gel, lithium heparin plasma. Protect from light.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 30 minutes of collection.  Transport to laboratory at 2-8 degrees C.

Stability (from collection to initiation)

Before centrifugation: Ambient, 1 hour.

After centrifugation in gel tube: Ambient, 8 hours; Refrigerated, 2 days; Frozen, Unacceptable.

Plasma aliquot: Ambient, 8 hours; Refrigerated, 2 days; Frozen, Unknown.

Frozen samples may be thawed one time only.

Remarks

Protect from light. Naproxen can result in spurious elevations in Total Bilirubin Levels.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Upon receipt

Methodology

Jendrassik-Grof

Reported

Same day

Synonyms

  • LAB50
  • BILI T
Result Interpretation

Reference Interval

Effective 05/20/2014:

0.1 - 1.3 mg/dL

Before 05/20/2014:

Sex From Age To Age Normal Low Normal High Units
M/F 0 1M 0.0 12.0 mg/dL
M/F 1M 150 0.0 1.3 mg/dL

Interpretive Data

Adult reference range.

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

82247

More Articles...