Tests Catalogs

ZIKA VIRUS - CDPHE/CDC

LAB8814

Collect

Answer ALL questions completely including:

Date of Symptoms Onset

Dates of Travel out of the Country

Updated by CDC Effective 7/14/2016:

For symptomatic patients:

Day 1-7 after symptom onset: Send urine (>=1.0mL) AND serum (0.3mL) for RT-PCR testing.

[CSF (>=1.0mL) OR amniotic fluid (0.5-1.0mL) can also be tested by RT-PCR in this time frame]

Day 8-14 after symptom onset: Send urine (>=1.0mL) for RT-PCR testing AND serum (>=1.0 mL) for MAC-ELISA (IgM antibody) testing

Day >=14 (but <12 weeks) after symptom onset: Send serum (>=1.0mL) for MAC-ELISA.

For asymptomatic pregnant women (Testing is by MAC-ELISA only):

Collect serum (>=1.0mL) OR amniotic fluid (>=1.0mL) from 2-12 weeks following travel to a Zika-affected area.

 

 

Specimen Preparation

Collect Red/Gold/SST OR Red No Gel for serum testing.

Collect urine, amniotic fluid, and CSF in sterile containers.

 

Storage/Transport Temperature

Refrigerate

Performed by

Effective 7/14/16:

RT-PCR performed at CDPHE (State Health Lab)

MAC-ELISA (IgM antibody) performed at CDPHE (State Health Lab)

If MAC-ELISA positive, PRNT confirmatory testing performed at CDC.

Notes

Please see the link on the right-hand side of the University Hospital Test Directory for the Zika MAC-ELISA Fact Sheets for Health Care Providers, Patients and Pregnant Women privded by the CDC for test result interpretation.

Synonyms

  • ZIKA
  • LAB8814
  • CDC

Performed by

Effective 7/14/16:

RT-PCR performed at CDPHE (State Health Lab)

MAC-ELISA (IgM antibody) performed at CDPHE (State Health Lab)

If MAC-ELISA positive, PRNT confirmatory testing performed at CDC.

CPT Codes

RT-PCR = 87798

MAC-ELISA = 86790

PRNT = 99001 (sample handling fee only)

Collection

LAB8814

Collect

Answer ALL questions completely including:

Date of Symptoms Onset

Dates of Travel out of the Country

Updated by CDC Effective 7/14/2016:

For symptomatic patients:

Day 1-7 after symptom onset: Send urine (>=1.0mL) AND serum (0.3mL) for RT-PCR testing.

[CSF (>=1.0mL) OR amniotic fluid (0.5-1.0mL) can also be tested by RT-PCR in this time frame]

Day 8-14 after symptom onset: Send urine (>=1.0mL) for RT-PCR testing AND serum (>=1.0 mL) for MAC-ELISA (IgM antibody) testing

Day >=14 (but <12 weeks) after symptom onset: Send serum (>=1.0mL) for MAC-ELISA.

For asymptomatic pregnant women (Testing is by MAC-ELISA only):

Collect serum (>=1.0mL) OR amniotic fluid (>=1.0mL) from 2-12 weeks following travel to a Zika-affected area.

 

 

Specimen Preparation

Collect Red/Gold/SST OR Red No Gel for serum testing.

Collect urine, amniotic fluid, and CSF in sterile containers.

 

Storage/Transport Temperature

Refrigerate

Performed by

Effective 7/14/16:

RT-PCR performed at CDPHE (State Health Lab)

MAC-ELISA (IgM antibody) performed at CDPHE (State Health Lab)

If MAC-ELISA positive, PRNT confirmatory testing performed at CDC.

Notes

Please see the link on the right-hand side of the University Hospital Test Directory for the Zika MAC-ELISA Fact Sheets for Health Care Providers, Patients and Pregnant Women privded by the CDC for test result interpretation.
Ordering

Synonyms

  • ZIKA
  • LAB8814
  • CDC
Result Interpretation

Performed by

Effective 7/14/16:

RT-PCR performed at CDPHE (State Health Lab)

MAC-ELISA (IgM antibody) performed at CDPHE (State Health Lab)

If MAC-ELISA positive, PRNT confirmatory testing performed at CDC.

Administrative

CPT Codes

RT-PCR = 87798

MAC-ELISA = 86790

PRNT = 99001 (sample handling fee only)

ZINC PROTOPORPHYRINS

LAB1085

Collect

Lavender (EDTA); Royal blue, (EDTA), tan (EDTA), or pink (K2 EDTA). Use royal blue (EDTA) tube when also testing for lead.

Specimen Preparation

Transport 1 mL whole blood. (Min: 0.2 mL)

Unacceptable Conditions

Specimens not collected in EDTA. Clotted, frozen, or hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 30 hours; Refrigerated: 5 weeks; Frozen: Unacceptable

Notes

Elevated ZPP results are seen in early and late iron deficiency, the anemia of chronic disease, chronic lead poisoning, and erythropoietic protoporphyria.  Elevated bilirubin or riboflavin and hemolyzed, clotted, or improperly aliquoted specimens may falsely increase the ZPP concentration.


A more specific test for free protoporphyrin is Porphyrins, Serum Total (0080429).  Erythrocyte Porphyrin (EP), Whole Blood (0020610), measures free protoporphyrin and zinc protoporphyrin.

Performed

Mon-Fri

Methodology

Quantitative Hematofluorometry

Reported

1-4 days

Synonyms

  • Protoporphyrin, Zinc
  • Porphyrins, Whole Blood (ZPP)
  • ZPP, Whole Blood
  • LAB1085

Additional Technical Information

Ordering Recommendations

• Useful as an indicator of chronic exposure to lead, primarily in the industrial setting. • For iron deficiency assessment - Iron and Iron Binding Capacity (0020420) and Ferritin (0070065) are recommended. • For lead exposure assessment - Lead Blood (Venous) (0020098) is preferred. • For occupational exposure to lead assessment - Zinc Protoporphyrin (ZPP) Whole Blood Industrial (0020614) or Lead Industrial Exposure Panel, Adults (0025016) are recommended.

Reference Interval

0-69 µmol ZPP/ mol Hem

CPT Codes

84202
Collection

LAB1085

Collect

Lavender (EDTA); Royal blue, (EDTA), tan (EDTA), or pink (K2 EDTA). Use royal blue (EDTA) tube when also testing for lead.

Specimen Preparation

Transport 1 mL whole blood. (Min: 0.2 mL)

Unacceptable Conditions

Specimens not collected in EDTA. Clotted, frozen, or hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Ambient: 30 hours; Refrigerated: 5 weeks; Frozen: Unacceptable

Notes

Elevated ZPP results are seen in early and late iron deficiency, the anemia of chronic disease, chronic lead poisoning, and erythropoietic protoporphyria.  Elevated bilirubin or riboflavin and hemolyzed, clotted, or improperly aliquoted specimens may falsely increase the ZPP concentration.


A more specific test for free protoporphyrin is Porphyrins, Serum Total (0080429).  Erythrocyte Porphyrin (EP), Whole Blood (0020610), measures free protoporphyrin and zinc protoporphyrin.
Ordering

Performed

Mon-Fri

Methodology

Quantitative Hematofluorometry

Reported

1-4 days

Synonyms

  • Protoporphyrin, Zinc
  • Porphyrins, Whole Blood (ZPP)
  • ZPP, Whole Blood
  • LAB1085

Additional Technical Information

Ordering Recommendations

• Useful as an indicator of chronic exposure to lead, primarily in the industrial setting. • For iron deficiency assessment - Iron and Iron Binding Capacity (0020420) and Ferritin (0070065) are recommended. • For lead exposure assessment - Lead Blood (Venous) (0020098) is preferred. • For occupational exposure to lead assessment - Zinc Protoporphyrin (ZPP) Whole Blood Industrial (0020614) or Lead Industrial Exposure Panel, Adults (0025016) are recommended.
Result Interpretation

Reference Interval

0-69 µmol ZPP/ mol Hem
Administrative

CPT Codes

84202

ZINC, SERUM [PLASMA]

LAB581

Collect

Royal blue (no additives), or royal blue (EDTA).

Patient Preparation

Diet, medication, and nutritional supplements may introduce interfering substances. Upon the advice of their physician, patients should be encouraged to discontinue nutritional supplements, vitamins, minerals, and nonessential over-the-counter medications for one week prior to sample draw.

Specimen Preparation

Do not allow serum or plasma to remain on cells. Centrifuge and pour off serum or plasma ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Trace Element-Free Transport Tube (ARUP supply #43116). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 0.5 mL)

Unacceptable Conditions

Separator tubes. Specimens that are not separated from the red cells or clot. Hemolyzed specimens.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated or frozen.

Stability (from collection to initiation)

If the specimen is drawn and stored in the appropriate container, the trace element values do not change with time.

Notes

Elevated results from noncertified trace element-free tubes may be due to contamination.  Elevated concentrations of trace elements in serum should be confirmed with a second specimen collected in a trace element-free tube, such as royal blue sterile tube (no additive).

Performed

Sun-Sat

Methodology

Quantitative Inductively Coupled Plasma-Mass Spectrometry

Reported

1-2 days

Additional Technical Information

Ordering Recommendations

May be useful as an indicator of acute deficiency. For acute toxicity, Zinc, Urine (0020462) may be a more reliable indicator of exposure.

Reference Interval

60-120 µg/dL

Interpretive Data

Circulating zinc concentrations are dependent on albumin status and are depressed with malnutrition.  Zinc may also be lowered with infection, inflammation, stress, oral contraceptives, and pregnancy.  Zinc may be elevated with zinc supplementation or fasting.  Elevated zinc concentrations may interfere with copper absorption.

CPT Codes

84630
Collection

LAB581

Collect

Royal blue (no additives), or royal blue (EDTA).

Patient Preparation

Diet, medication, and nutritional supplements may introduce interfering substances. Upon the advice of their physician, patients should be encouraged to discontinue nutritional supplements, vitamins, minerals, and nonessential over-the-counter medications for one week prior to sample draw.

Specimen Preparation

Do not allow serum or plasma to remain on cells. Centrifuge and pour off serum or plasma ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Trace Element-Free Transport Tube (ARUP supply #43116). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 0.5 mL)

Unacceptable Conditions

Separator tubes. Specimens that are not separated from the red cells or clot. Hemolyzed specimens.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated or frozen.

Stability (from collection to initiation)

If the specimen is drawn and stored in the appropriate container, the trace element values do not change with time.

Notes

Elevated results from noncertified trace element-free tubes may be due to contamination.  Elevated concentrations of trace elements in serum should be confirmed with a second specimen collected in a trace element-free tube, such as royal blue sterile tube (no additive).
Ordering

Performed

Sun-Sat

Methodology

Quantitative Inductively Coupled Plasma-Mass Spectrometry

Reported

1-2 days

Additional Technical Information

Ordering Recommendations

May be useful as an indicator of acute deficiency. For acute toxicity, Zinc, Urine (0020462) may be a more reliable indicator of exposure.
Result Interpretation

Reference Interval

60-120 µg/dL

Interpretive Data

Circulating zinc concentrations are dependent on albumin status and are depressed with malnutrition.  Zinc may also be lowered with infection, inflammation, stress, oral contraceptives, and pregnancy.  Zinc may be elevated with zinc supplementation or fasting.  Elevated zinc concentrations may interfere with copper absorption.

Administrative

CPT Codes

84630

ZONISAMIDE LEVEL

LAB504

 

Collect

Plain red. Also acceptable: Lavender (K2 or K3EDTA) or pink (K2 EDTA).

Patient Preparation

Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration.

Specimen Preparation

Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution).

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

After separation from cells: Ambient: 1 week; Refrigerated: 1 week; Frozen: 6 weeks

Performed

Mon-Fri

Methodology

Quantitative Enzyme Multiplied Immunoassay Technique

Reported

1-4 days

Synonyms

  • Zonegran

Ordering Recommendations

Optimize drug therapy and monitor patient adherence.

Reference Interval

Effective February 19, 2013
Therapeutic RangeNot well established
Toxic LevelGreater than 80 µg/mL

Interpretive Data

The proposed therapeutic range for seizure control is 10-40 µg/mL. Toxic concentrations may cause coma, seizures and cardiac abnormalities. Pharmacokinetics varies widely, particularly with co-medications and/or compromised renal function.

CPT Codes

80203 (Alt code: G0480)
Collection

LAB504

 

Collect

Plain red. Also acceptable: Lavender (K2 or K3EDTA) or pink (K2 EDTA).

Patient Preparation

Timing of specimen collection: Pre-dose (trough) draw - At steady state concentration.

Specimen Preparation

Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution).

Storage/Transport Temperature

Refrigerated

Stability (from collection to initiation)

After separation from cells: Ambient: 1 week; Refrigerated: 1 week; Frozen: 6 weeks
Ordering

Performed

Mon-Fri

Methodology

Quantitative Enzyme Multiplied Immunoassay Technique

Reported

1-4 days

Synonyms

  • Zonegran

Ordering Recommendations

Optimize drug therapy and monitor patient adherence.
Result Interpretation

Reference Interval

Effective February 19, 2013
Therapeutic RangeNot well established
Toxic LevelGreater than 80 µg/mL

Interpretive Data

The proposed therapeutic range for seizure control is 10-40 µg/mL. Toxic concentrations may cause coma, seizures and cardiac abnormalities. Pharmacokinetics varies widely, particularly with co-medications and/or compromised renal function.

Administrative

CPT Codes

80203 (Alt code: G0480)