T AND B CELL PANEL

LAB3053

TBCP

Collect

One 4 or 6 mL Green top tube (sodium heparin) AND one 4 mL Purple top tube (EDTA).

Dark Green and EDTA must be collected at same draw.

Pediatric Collection

3 mL Green top tube (sodium heparin) AND 3 mL Purple top tube (EDTA) or microtainer (EDTA).

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; specimens older than 24 hours; clotted, frozen, or hemolyzed specimens.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: Transport at ambient temperature.

Stability (from collection to initiation)

Ambient: Green top/purple top tubes for lymphocyte subset analysis 24 hours; Purple top tube for CBC 8 hours.

 

Refrigerated: Purple top tube for CBC 36 hours.

Remarks

T and B Cell panel requires an Absolute Lymphocyte Count. A Complete Blood Count (CBC) and differential (DIFF) must be ordered. CPT code 85025 or 85027 and 85007 will be billed as needed.

Performed by

PCL Clinical Laboratory - Flow Cytometry

Performed

Daily

Methodology

Flow Cytometry

Reported

Same day

Synonyms

  • LAB3053
  • TBCP

Reference Interval

Component Sex From Age To Age Normal Low Normal High Units
B CELLS: %CD19+ M/F 3 21 11 45 %
M/F 21 150 1 12 %
B CELLS: ABS CD19+ M/F 3 21 432 3345 /uL
M/F 21 150 42 400 /uL
CD4:CD8 RATIO M/F 0 150 1.0 3.3 %
HELPER T CELLS: %CD3+CD4+ M/F 3 21 33 44 %
M/F 21 150 32 66 %
HELPER T CELLS: ABS CD3+CD4+ M/F 3 21 1216 2009 /uL
M/F 21 150 389 1868 /uL
SUPPRESSOR T CELL:ABS CD3+CD8+ M/F 3 21 351 2479 /uL
M/F 21 150 185 1049 /uL
SUPPRESSOR T CELLS: %CD3+CD8+ M/F 3 21 8 31 %
M/F 21 150 14 42 %
TOTAL T CELLS: %CD3+ M/F 3 21 55 82 %
M/F 21 150 63 86 %
TOTAL T CELLS: ABS CD3+ M/F 3 21 2649 3639 /uL
M/F 21 150 760 2130 /uL

Performed by

PCL Clinical Laboratory - Flow Cytometry

CPT Codes

86355, 86359, 86360

Collection

LAB3053

TBCP

Collect

One 4 or 6 mL Green top tube (sodium heparin) AND one 4 mL Purple top tube (EDTA).

Dark Green and EDTA must be collected at same draw.

Pediatric Collection

3 mL Green top tube (sodium heparin) AND 3 mL Purple top tube (EDTA) or microtainer (EDTA).

Unacceptable Conditions

Specimen not properly identified; incorrect container; insufficient sample volume; specimens older than 24 hours; clotted, frozen, or hemolyzed specimens.

Storage/Transport Temperature

Internal: Deliver to lab immediately
Offsite: Transport at ambient temperature.

Stability (from collection to initiation)

Ambient: Green top/purple top tubes for lymphocyte subset analysis 24 hours; Purple top tube for CBC 8 hours.

 

Refrigerated: Purple top tube for CBC 36 hours.

Remarks

T and B Cell panel requires an Absolute Lymphocyte Count. A Complete Blood Count (CBC) and differential (DIFF) must be ordered. CPT code 85025 or 85027 and 85007 will be billed as needed.

Performed by

PCL Clinical Laboratory - Flow Cytometry

Ordering

Performed

Daily

Methodology

Flow Cytometry

Reported

Same day

Synonyms

  • LAB3053
  • TBCP
Result Interpretation

Reference Interval

Component Sex From Age To Age Normal Low Normal High Units
B CELLS: %CD19+ M/F 3 21 11 45 %
M/F 21 150 1 12 %
B CELLS: ABS CD19+ M/F 3 21 432 3345 /uL
M/F 21 150 42 400 /uL
CD4:CD8 RATIO M/F 0 150 1.0 3.3 %
HELPER T CELLS: %CD3+CD4+ M/F 3 21 33 44 %
M/F 21 150 32 66 %
HELPER T CELLS: ABS CD3+CD4+ M/F 3 21 1216 2009 /uL
M/F 21 150 389 1868 /uL
SUPPRESSOR T CELL:ABS CD3+CD8+ M/F 3 21 351 2479 /uL
M/F 21 150 185 1049 /uL
SUPPRESSOR T CELLS: %CD3+CD8+ M/F 3 21 8 31 %
M/F 21 150 14 42 %
TOTAL T CELLS: %CD3+ M/F 3 21 55 82 %
M/F 21 150 63 86 %
TOTAL T CELLS: ABS CD3+ M/F 3 21 2649 3639 /uL
M/F 21 150 760 2130 /uL

Performed by

PCL Clinical Laboratory - Flow Cytometry

Administrative

CPT Codes

86355, 86359, 86360

T CELL GAMMA RECEPTOR CLONALITY BY PCR

LAB3092

Collect

3 mL purple top [EDTA] - whole blood or bone marrow in EDTA; CSF

Unacceptable Conditions

Samples in heparinized anticoagulant. Frozen samples.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 1 mL blood, bone marrow, CSF Deliver to lab at ambient temperature

Stability (from collection to initiation)

3 days at ambient temperature.

Remarks

Store blood/bone marrow at ambient temperature until shipped to reference lab. Store CSF refrigerated.

Performed by

PCL Clinical Laboratory

Performed

Weekly (Dayshift), day varies

Methodology

Polymerase Chain Reaction (PCR)

Reported

7 - 10 days

Synonyms

  • LAB3092

Performed by

PCL Clinical Laboratory

CPT Codes

81342

Collection

LAB3092

Collect

3 mL purple top [EDTA] - whole blood or bone marrow in EDTA; CSF

Unacceptable Conditions

Samples in heparinized anticoagulant. Frozen samples.

Storage/Transport Temperature

Internal: Deliver to lab immediatley
Offsite: 1 mL blood, bone marrow, CSF Deliver to lab at ambient temperature

Stability (from collection to initiation)

3 days at ambient temperature.

Remarks

Store blood/bone marrow at ambient temperature until shipped to reference lab. Store CSF refrigerated.

Performed by

PCL Clinical Laboratory

Ordering

Performed

Weekly (Dayshift), day varies

Methodology

Polymerase Chain Reaction (PCR)

Reported

7 - 10 days

Synonyms

  • LAB3092
Result Interpretation

Performed by

PCL Clinical Laboratory

Administrative

CPT Codes

81342

T3 (TRIIODOTHYRONINE) UPTAKE

LAB4080

Collect

Serum separator tube. Also acceptable: Plasma separator tube, lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Allow serum to clot completely at room temperature. Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation of cells: 8 days; Refrigerated: 2 weeks; Frozen: 2 years

Notes

T3 uptake is of little clinical value alone; it is used to determine the Free Thyroxine Index.

Performed

Sun-Sat

Methodology

Quantitative Electrochemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • THBR
  • Thyroid Binding Ratio
  • T-Uptake
  • Triiodothyronine Uptake, Serum
  • T3U, Serum
  • T3 Uptake, S
  • LAB4080
  • T3 UP

Ordering Recommendations

Not recommended for routine thyroid screening.

Reference Interval

28-41% uptake

Interpretive Data

Thyroxine, Free (Free T4) (0070138) is the preferred test alternative for T3 uptake and Free Thyroxine Index tests.

CPT Codes

84479
Collection

LAB4080

Collect

Serum separator tube. Also acceptable: Plasma separator tube, lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Allow serum to clot completely at room temperature. Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Unacceptable Conditions

Grossly hemolyzed specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation of cells: 8 days; Refrigerated: 2 weeks; Frozen: 2 years

Notes

T3 uptake is of little clinical value alone; it is used to determine the Free Thyroxine Index.
Ordering

Performed

Sun-Sat

Methodology

Quantitative Electrochemiluminescent Immunoassay

Reported

Within 24 hours

Synonyms

  • THBR
  • Thyroid Binding Ratio
  • T-Uptake
  • Triiodothyronine Uptake, Serum
  • T3U, Serum
  • T3 Uptake, S
  • LAB4080
  • T3 UP

Ordering Recommendations

Not recommended for routine thyroid screening.
Result Interpretation

Reference Interval

28-41% uptake

Interpretive Data

Thyroxine, Free (Free T4) (0070138) is the preferred test alternative for T3 uptake and Free Thyroxine Index tests.

Administrative

CPT Codes

84479

T3 TOTAL

LAB136

T3

Collect

One 5.0 mL Gold top with gel separator (SST), (clot activator serum). Tube must filled to at least 90 % capacity.

Pediatric Collection

1.0 mL blood in a 6.0 mL red top no gel, serum (clot activator).

Unacceptable Conditions

Anticoagulated samples; specimen not properly identified; insufficient sample volume; sample frozen and thawed more than once.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Allow to clot in an upright position for 30 minutes prior to centrifugation.Centrifuge within 60 minutes of collection. Send at 2-8 degrees C if tested within 48 hours. Place serum in an aliquot container and send frozen if not tested within 48 hours.

Stability (from collection to initiation)

After centrifugation: Ambient, 8 hours; Refrigerated, 48 hours; Frozen (-20 degrees C), Unacceptable.

Serum aliquot: Ambient, 8 hours; Refrigerated, 48 hours; Frozen (-20 degrees C), Greater than 48 hours.

Performed by

PCL Clinical Laboratory - Core

Performed

Upon receipt

Methodology

Chemiluminescent Immunoassay

Reported

Same day

Synonyms

  • triiodothyronine
  • LAB136
  • T3

Reference Interval

60 - 181 ng/dL

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84480

Collection

LAB136

T3

Collect

One 5.0 mL Gold top with gel separator (SST), (clot activator serum). Tube must filled to at least 90 % capacity.

Pediatric Collection

1.0 mL blood in a 6.0 mL red top no gel, serum (clot activator).

Unacceptable Conditions

Anticoagulated samples; specimen not properly identified; insufficient sample volume; sample frozen and thawed more than once.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Allow to clot in an upright position for 30 minutes prior to centrifugation.Centrifuge within 60 minutes of collection. Send at 2-8 degrees C if tested within 48 hours. Place serum in an aliquot container and send frozen if not tested within 48 hours.

Stability (from collection to initiation)

After centrifugation: Ambient, 8 hours; Refrigerated, 48 hours; Frozen (-20 degrees C), Unacceptable.

Serum aliquot: Ambient, 8 hours; Refrigerated, 48 hours; Frozen (-20 degrees C), Greater than 48 hours.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Upon receipt

Methodology

Chemiluminescent Immunoassay

Reported

Same day

Synonyms

  • triiodothyronine
  • LAB136
  • T3
Result Interpretation

Reference Interval

60 - 181 ng/dL

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84480

T4 (THYROXINE) FREE

LAB127

FT4

Do NOT order this test for babies <9 months old. Order free T4 by Equilibrium Dialysis (LAB3569).

Collect

One 4.5 mL Green top tube with gel separator (PST), plasma (lithium heparin). Tube must filled to at least 90 % capacity.

 

 

Pediatric Collection

1.0 mL blood in a red top tube (microtainer without gel).

0.5mL serum

Unacceptable Conditions

Anticoagulant tube; tube not filled to at least 90% capacity; specimen not properly identified; insufficient sample volume; samples frozen and thawed more than once.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 60 minutes of collection. Send at 2-8 degrees C if tested within 48 hours. Place 0.5 mL plasma (or serum) in aliquot container and send frozen if not tested within 48 hours.

Stability (from collection to initiation)

 

After centrifugation: Ambient, 8 hours; Refrigerated, 48 hours; Frozen, unacceptable.

Plasma/serum aliquot: Ambient, 8 hours; Refrigerated, 48 hours; Frozen (-20 degrees C), Greater than 48 hours.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Chemiluminescent Immunoassay

Reported

Same day

Synonyms

  • LAB127
  • FT4

Reference Interval

0.89 - 1.76 ng/dL

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84439

Collection

LAB127

FT4

Do NOT order this test for babies <9 months old. Order free T4 by Equilibrium Dialysis (LAB3569).

Collect

One 4.5 mL Green top tube with gel separator (PST), plasma (lithium heparin). Tube must filled to at least 90 % capacity.

 

 

Pediatric Collection

1.0 mL blood in a red top tube (microtainer without gel).

0.5mL serum

Unacceptable Conditions

Anticoagulant tube; tube not filled to at least 90% capacity; specimen not properly identified; insufficient sample volume; samples frozen and thawed more than once.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Centrifuge within 60 minutes of collection. Send at 2-8 degrees C if tested within 48 hours. Place 0.5 mL plasma (or serum) in aliquot container and send frozen if not tested within 48 hours.

Stability (from collection to initiation)

 

After centrifugation: Ambient, 8 hours; Refrigerated, 48 hours; Frozen, unacceptable.

Plasma/serum aliquot: Ambient, 8 hours; Refrigerated, 48 hours; Frozen (-20 degrees C), Greater than 48 hours.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Chemiluminescent Immunoassay

Reported

Same day

Synonyms

  • LAB127
  • FT4
Result Interpretation

Reference Interval

0.89 - 1.76 ng/dL

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84439

T4 (THYROXINE) TOTAL

LAB126

T4

Collect

One 5.0 mL gold top with gel (SST), (clot activator serum). Tube must be filled to at least 90% capacity.

Pediatric Collection

At least 1.0 mL blood in a 6.0 mL red top no gel, serum (clot activator).

Unacceptable Conditions

Anticoagulated samples; samples frozen and thawed more than once; gold top tube not filled to at least 90% capacity; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal:Deliver to lab immediately.

 Offsite: Allow sample to clot in an upright position for 30 minutes prior to centrifugation. Centrifuge within 60 minutes of collection. Send at 2-8 degrees C if tested within 48 hours. Place 2 mL serum in an aliquot container and send frozen if not tested within 48 hours.

Stability (from collection to initiation)

After centrifugation: Ambient, 8 hours; Refrigerated, 48 hours; Frozen, unacceptable.

Serum aliquot: Ambient: 8 hours; Refrigerated: 48 hours; Frozen (-20 degrees C), Greater than 48 hours.

Performed by

PCL Clinical Laboratory - Core

Performed

Daily

Methodology

Chemiluminescent Immunoassay

Reported

Daily

Synonyms

  • LAB126

Reference Interval

4 - 11 ug/dL

Performed by

PCL Clinical Laboratory - Core

CPT Codes

84436

Collection

LAB126

T4

Collect

One 5.0 mL gold top with gel (SST), (clot activator serum). Tube must be filled to at least 90% capacity.

Pediatric Collection

At least 1.0 mL blood in a 6.0 mL red top no gel, serum (clot activator).

Unacceptable Conditions

Anticoagulated samples; samples frozen and thawed more than once; gold top tube not filled to at least 90% capacity; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal:Deliver to lab immediately.

 Offsite: Allow sample to clot in an upright position for 30 minutes prior to centrifugation. Centrifuge within 60 minutes of collection. Send at 2-8 degrees C if tested within 48 hours. Place 2 mL serum in an aliquot container and send frozen if not tested within 48 hours.

Stability (from collection to initiation)

After centrifugation: Ambient, 8 hours; Refrigerated, 48 hours; Frozen, unacceptable.

Serum aliquot: Ambient: 8 hours; Refrigerated: 48 hours; Frozen (-20 degrees C), Greater than 48 hours.

Performed by

PCL Clinical Laboratory - Core

Ordering

Performed

Daily

Methodology

Chemiluminescent Immunoassay

Reported

Daily

Synonyms

  • LAB126
Result Interpretation

Reference Interval

4 - 11 ug/dL

Performed by

PCL Clinical Laboratory - Core

Administrative

CPT Codes

84436

T4 FREE BY EQUIL DIALYSIS-TMS

LAB3569

 

Order this test for Free T4 testing for babies <9 months old.

Pediatric Collection: 1ml blood (0.5ml serum)

Collect

Plain red or serum separator tube (SST).

Specimen Preparation

Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Plasma.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 4 days; Refrigerated: 2 weeks; Frozen: 1 month

Performed

Sun, Tue, Thu

Methodology

Quantitative Equilibrium Dialysis/High Performance Liquid Chromatography-Tandem Mass Spectrometry

Reported

2-5 days

Synonyms

  • T4, Free, Equilibrium Dialysis
  • LAB3569
  • Free T4 by Equilibrium Dialysis

Ordering Recommendations

Not recommended for routine evaluation of thyroid disorders. Order Thyroxine, Free (Free FT4) (0070138), instead.

Reference Interval

Effective May 16, 2011
Free Thyroxine ng/dL
AgeFemaleMale
25-30 weeks gestation0.5-3.3 ng/dL0.5-3.3 ng/dL
31-36 weeks gestation1.3-4.7 ng/dL1.3-4.7 ng/dL
Birth to 1 week2.2-5.3 ng/dL2.2-5.3 ng/dL
2-3 weeks0.9-4.0 ng/dL0.9-4.0 ng/dL
1-5 months1.1-2.2 ng/dL1.1-2.2 ng/dL
6 months-6 years1.4-2.7 ng/dL1.4-2.7 ng/dL
7 years-17 years1.1-2.0 ng/dL1.1-2.0 ng/dL
18 years and older1.1-2.4 ng/dL1.1-2.4 ng/dL
Pregnancy, 1st Trimester0.7-2.0 ng/dL
Pregnancy, 2nd Trimester0.7-2.1 ng/dL
Pregnancy, 3rd Trimester0.5-1.6 ng/dL

CPT Codes

84439
Collection

LAB3569

 

Order this test for Free T4 testing for babies <9 months old.

Pediatric Collection: 1ml blood (0.5ml serum)

Collect

Plain red or serum separator tube (SST).

Specimen Preparation

Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Unacceptable Conditions

Plasma.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 4 days; Refrigerated: 2 weeks; Frozen: 1 month
Ordering

Performed

Sun, Tue, Thu

Methodology

Quantitative Equilibrium Dialysis/High Performance Liquid Chromatography-Tandem Mass Spectrometry

Reported

2-5 days

Synonyms

  • T4, Free, Equilibrium Dialysis
  • LAB3569
  • Free T4 by Equilibrium Dialysis

Ordering Recommendations

Not recommended for routine evaluation of thyroid disorders. Order Thyroxine, Free (Free FT4) (0070138), instead.
Result Interpretation

Reference Interval

Effective May 16, 2011
Free Thyroxine ng/dL
AgeFemaleMale
25-30 weeks gestation0.5-3.3 ng/dL0.5-3.3 ng/dL
31-36 weeks gestation1.3-4.7 ng/dL1.3-4.7 ng/dL
Birth to 1 week2.2-5.3 ng/dL2.2-5.3 ng/dL
2-3 weeks0.9-4.0 ng/dL0.9-4.0 ng/dL
1-5 months1.1-2.2 ng/dL1.1-2.2 ng/dL
6 months-6 years1.4-2.7 ng/dL1.4-2.7 ng/dL
7 years-17 years1.1-2.0 ng/dL1.1-2.0 ng/dL
18 years and older1.1-2.4 ng/dL1.1-2.4 ng/dL
Pregnancy, 1st Trimester0.7-2.0 ng/dL
Pregnancy, 2nd Trimester0.7-2.1 ng/dL
Pregnancy, 3rd Trimester0.5-1.6 ng/dL

Administrative

CPT Codes

84439

TACROLIMUS

LAB876

Collect

One 4.0 mL purple top (EDTA whole blood).

Pediatric Collection

Minimum volume: 1 mL EDTA whole blood.

Unacceptable Conditions

Specimen improperly stored; centrifuged sample; obvious microbial contamination; heat-inactivated sample; cadaver sample; other body fluids; specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Do not centrifuge. Deliver to laboratory refrigerated/on ice (2-8 degrees C).

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated (2-8 degrees C): 7 days; Frozen (-10 degrees C or lower): 6 months.

Remarks

Do not centrifuge.

Specimen should be a trough level; draw immediately prior to next dose.

Performed by

PCL Clinical Laboratory - Toxicology

Performed

Daily: Run Times 

In Lab By     Reported        
 07:00  10:30
 11:00  13:30
 15:00  16:30
 After 15:00  10:30 next day

Methodology

Chemiluminescent microparticle immunoassay (CMIA)

Synonyms

  • FK506
  • Prograf
  • -337
  • LAB876

Reference Interval

8-18 ng/mL

Performed by

PCL Clinical Laboratory - Toxicology

CPT Codes

80197

Collection

LAB876

Collect

One 4.0 mL purple top (EDTA whole blood).

Pediatric Collection

Minimum volume: 1 mL EDTA whole blood.

Unacceptable Conditions

Specimen improperly stored; centrifuged sample; obvious microbial contamination; heat-inactivated sample; cadaver sample; other body fluids; specimen not properly identified; incorrect container; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Do not centrifuge. Deliver to laboratory refrigerated/on ice (2-8 degrees C).

Stability (from collection to initiation)

Ambient: 24 hours; Refrigerated (2-8 degrees C): 7 days; Frozen (-10 degrees C or lower): 6 months.

Remarks

Do not centrifuge.

Specimen should be a trough level; draw immediately prior to next dose.

Performed by

PCL Clinical Laboratory - Toxicology

Ordering

Performed

Daily: Run Times 

In Lab By     Reported        
 07:00  10:30
 11:00  13:30
 15:00  16:30
 After 15:00  10:30 next day

Methodology

Chemiluminescent microparticle immunoassay (CMIA)

Synonyms

  • FK506
  • Prograf
  • -337
  • LAB876
Result Interpretation

Reference Interval

8-18 ng/mL

Performed by

PCL Clinical Laboratory - Toxicology

Administrative

CPT Codes

80197

TARTRATE RESISTANT ACID PHOSPHATASE

LAB3185

Collect

7.0 mL Red with no gel.

Patient Preparation

Effective 6/22/12 TRAP testing changed to quantitative test.

See new specimen requirements.

Pediatric Collection

Minimum volume = 1.0 mL serum

Storage/Transport Temperature

Spin and centrifuge. Store frozen.

Stability (from collection to initiation)

Frozen.

Performed by

Emory Genetics Laboratories

Performed

Varies

Methodology

Enzymatic reaction detected by Spectrophotometric Assay to measure TRAP activity.

Reported

In 6 days

Synonyms

  • Non-prostatic Acid Phosphatase
  • Acid Phosphatase, Tartrate Resistant
  • TRAP
  • TRAP - Gaucher Disease Biomarker
  • LAB3185

Reference Interval

Normal Range:  0.28-9.84 IU/L

(5.1 +/-4.8, n=23)  Median 4.6 IU/L

Performed by

Emory Genetics Laboratories

CPT Codes

82657

Collection

LAB3185

Collect

7.0 mL Red with no gel.

Patient Preparation

Effective 6/22/12 TRAP testing changed to quantitative test.

See new specimen requirements.

Pediatric Collection

Minimum volume = 1.0 mL serum

Storage/Transport Temperature

Spin and centrifuge. Store frozen.

Stability (from collection to initiation)

Frozen.

Performed by

Emory Genetics Laboratories

Ordering

Performed

Varies

Methodology

Enzymatic reaction detected by Spectrophotometric Assay to measure TRAP activity.

Reported

In 6 days

Synonyms

  • Non-prostatic Acid Phosphatase
  • Acid Phosphatase, Tartrate Resistant
  • TRAP
  • TRAP - Gaucher Disease Biomarker
  • LAB3185
Result Interpretation

Reference Interval

Normal Range:  0.28-9.84 IU/L

(5.1 +/-4.8, n=23)  Median 4.6 IU/L

Performed by

Emory Genetics Laboratories

Administrative

CPT Codes

82657

TAYSACHS CARRIER TEST-NONPREG

LAB3486

Collect

One 10 mL plain red from nonpregnant patient.

Storage/Transport Temperature

1 mL serum, frozen. (Min: 0.5 mL). Separate serum from cells ASAP.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 week

Performed by

Mayo Medical Laboratories

Notes

Diagnosing Tay-Sachs disease, carriers of Tay-Sachs, Sandhoff's disease, and carriers of Sandhoff's disease

Performed

Tues

Methodology

Heat Inactivation, Fluorometric, Automated

The hexosaminidases are among the more active of the lysosomal enzymes, which hydrolyze derivatives of beta-D-N-acetylglucosamine and beta-D-N-acetylgalactosamine. Natural substrates are certain sphingolipids (ie, GM2) in which acetylgalactosamine is the terminal monosaccharide. The two hexosaminidase isoenzymes, A and B, differ in their electrophoretic mobility and heat stability. Hexosaminidase A moves toward the anode and is heat labile, while hexosaminidase B moves toward the cathode and is heat stable.

 

The procedure is performed on a two channel Autoanalyzer equipped with two fluorometers. The substrate used is 4-methylumbelliferyl-N-acetyl-beta-D-glucopyranoside (4-MUF-acetamido-2-deoxy-beta-D-glucopyranoside) from which the fluorescent compound, 4-methylumbelliferone, is liberated by both hexosaminidases.

 

After sample pickup, a sample splitter divides the patient serum. One-half of the sample travels through a 37 degree C mixing coil with substrate. The other half is first directed through a 53.25 degree C heating coil for 5.5 minutes and is then mixed with substrate and sent through the 37 degree C mixing coil. The hexosaminidase A fraction is destroyed in the heated sample, leaving only hexosaminidase B to react with the substrate. The unheated sample provides the total hexosaminidase (A and B). The reactants are pumped through a fluorometer, and the intensity of the fluorescence is converted to peaks of varying heights on a recorder. Sample peaks are compared to that of a 100 microM beta-methylumbelliferone standard to quantitate both the total and the "B" fraction. The percentage of the "A" fraction that was inactivated by heating is calculated based on these results. The difference in heat inactivation is used to fractionate hexosaminidase activities.(O'Brien JF: Lysosomal storage diseases. In Tietz Textbook of Clinical Chemistry. Edited by CA Burtis, ER Ashwood. Second edition. Philadelphia, PA, WB Saunders Company, 1994. pp 2149-2160)

Reported

Within 7 days

Synonyms

  • Hexosaminidase, Total
  • Hex A Deficiency
  • Sandhoff Carrier Screening
  • Sandhoff Disease
  • Tay-Sachs Carrier Screening
  • LAB3486

Reference Interval

TOTAL:

<= 15 years:   >= 20 nmol/min/mL

>= 16 years:  10.4 - 23.8 nmol/min/mL

HEXOSAMINIDASE %A

<= 15 years:  20 - 90%

>= 16 years:  56 - 80%

Interpretive Data

By Report

Performed by

Mayo Medical Laboratories

CPT Codes

83080

Collection

LAB3486

Collect

One 10 mL plain red from nonpregnant patient.

Storage/Transport Temperature

1 mL serum, frozen. (Min: 0.5 mL). Separate serum from cells ASAP.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 week

Performed by

Mayo Medical Laboratories

Notes

Diagnosing Tay-Sachs disease, carriers of Tay-Sachs, Sandhoff's disease, and carriers of Sandhoff's disease

Ordering

Performed

Tues

Methodology

Heat Inactivation, Fluorometric, Automated

The hexosaminidases are among the more active of the lysosomal enzymes, which hydrolyze derivatives of beta-D-N-acetylglucosamine and beta-D-N-acetylgalactosamine. Natural substrates are certain sphingolipids (ie, GM2) in which acetylgalactosamine is the terminal monosaccharide. The two hexosaminidase isoenzymes, A and B, differ in their electrophoretic mobility and heat stability. Hexosaminidase A moves toward the anode and is heat labile, while hexosaminidase B moves toward the cathode and is heat stable.

 

The procedure is performed on a two channel Autoanalyzer equipped with two fluorometers. The substrate used is 4-methylumbelliferyl-N-acetyl-beta-D-glucopyranoside (4-MUF-acetamido-2-deoxy-beta-D-glucopyranoside) from which the fluorescent compound, 4-methylumbelliferone, is liberated by both hexosaminidases.

 

After sample pickup, a sample splitter divides the patient serum. One-half of the sample travels through a 37 degree C mixing coil with substrate. The other half is first directed through a 53.25 degree C heating coil for 5.5 minutes and is then mixed with substrate and sent through the 37 degree C mixing coil. The hexosaminidase A fraction is destroyed in the heated sample, leaving only hexosaminidase B to react with the substrate. The unheated sample provides the total hexosaminidase (A and B). The reactants are pumped through a fluorometer, and the intensity of the fluorescence is converted to peaks of varying heights on a recorder. Sample peaks are compared to that of a 100 microM beta-methylumbelliferone standard to quantitate both the total and the "B" fraction. The percentage of the "A" fraction that was inactivated by heating is calculated based on these results. The difference in heat inactivation is used to fractionate hexosaminidase activities.(O'Brien JF: Lysosomal storage diseases. In Tietz Textbook of Clinical Chemistry. Edited by CA Burtis, ER Ashwood. Second edition. Philadelphia, PA, WB Saunders Company, 1994. pp 2149-2160)

Reported

Within 7 days

Synonyms

  • Hexosaminidase, Total
  • Hex A Deficiency
  • Sandhoff Carrier Screening
  • Sandhoff Disease
  • Tay-Sachs Carrier Screening
  • LAB3486
Result Interpretation

Reference Interval

TOTAL:

<= 15 years:   >= 20 nmol/min/mL

>= 16 years:  10.4 - 23.8 nmol/min/mL

HEXOSAMINIDASE %A

<= 15 years:  20 - 90%

>= 16 years:  56 - 80%

Interpretive Data

By Report

Performed by

Mayo Medical Laboratories

Administrative

CPT Codes

83080

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