LAB3053
TBCP
Collect
One 4 or 6 mL Green top tube (sodium heparin) AND one 4 mL Purple top tube (EDTA).
Dark Green and EDTA must be collected at same draw.
Pediatric Collection
3 mL Green top tube (sodium heparin) AND 3 mL Purple top tube (EDTA) or microtainer (EDTA).
Unacceptable Conditions
Specimen not properly identified; incorrect container; insufficient sample volume; specimens older than 24 hours; clotted, frozen, or hemolyzed specimens.
Storage/Transport Temperature
Internal: Deliver to lab immediately
Offsite: Transport at ambient temperature.
Stability (from collection to initiation)
Ambient: Green top/purple top tubes for lymphocyte subset analysis 24 hours; Purple top tube for CBC 8 hours.
Refrigerated: Purple top tube for CBC 36 hours.
Remarks
T and B Cell panel requires an Absolute Lymphocyte Count. A Complete Blood Count (CBC) and differential (DIFF) must be ordered. CPT code 85025 or 85027 and 85007 will be billed as needed.
Performed by
PCL Clinical Laboratory - Flow Cytometry
Performed
Daily
Methodology
Flow Cytometry
Reported
Same day
Synonyms
- LAB3053
- TBCP
Reference Interval
Component | Sex | From Age | To Age | Normal Low | Normal High | Units |
B CELLS: %CD19+ | M/F | 3 | 21 | 11 | 45 | % |
M/F | 21 | 150 | 1 | 12 | % | |
B CELLS: ABS CD19+ | M/F | 3 | 21 | 432 | 3345 | /uL |
M/F | 21 | 150 | 42 | 400 | /uL | |
CD4:CD8 RATIO | M/F | 0 | 150 | 1.0 | 3.3 | % |
HELPER T CELLS: %CD3+CD4+ | M/F | 3 | 21 | 33 | 44 | % |
M/F | 21 | 150 | 32 | 66 | % | |
HELPER T CELLS: ABS CD3+CD4+ | M/F | 3 | 21 | 1216 | 2009 | /uL |
M/F | 21 | 150 | 389 | 1868 | /uL | |
SUPPRESSOR T CELL:ABS CD3+CD8+ | M/F | 3 | 21 | 351 | 2479 | /uL |
M/F | 21 | 150 | 185 | 1049 | /uL | |
SUPPRESSOR T CELLS: %CD3+CD8+ | M/F | 3 | 21 | 8 | 31 | % |
M/F | 21 | 150 | 14 | 42 | % | |
TOTAL T CELLS: %CD3+ | M/F | 3 | 21 | 55 | 82 | % |
M/F | 21 | 150 | 63 | 86 | % | |
TOTAL T CELLS: ABS CD3+ | M/F | 3 | 21 | 2649 | 3639 | /uL |
M/F | 21 | 150 | 760 | 2130 | /uL |
Performed by
PCL Clinical Laboratory - Flow Cytometry
CPT Codes
86355, 86359, 86360
Collection |
LAB3053
TBCP
Collect
One 4 or 6 mL Green top tube (sodium heparin) AND one 4 mL Purple top tube (EDTA).
Dark Green and EDTA must be collected at same draw.
Pediatric Collection
3 mL Green top tube (sodium heparin) AND 3 mL Purple top tube (EDTA) or microtainer (EDTA).
Unacceptable Conditions
Specimen not properly identified; incorrect container; insufficient sample volume; specimens older than 24 hours; clotted, frozen, or hemolyzed specimens.
Storage/Transport Temperature
Internal: Deliver to lab immediately
Offsite: Transport at ambient temperature.
Stability (from collection to initiation)
Ambient: Green top/purple top tubes for lymphocyte subset analysis 24 hours; Purple top tube for CBC 8 hours.
Refrigerated: Purple top tube for CBC 36 hours.
Remarks
T and B Cell panel requires an Absolute Lymphocyte Count. A Complete Blood Count (CBC) and differential (DIFF) must be ordered. CPT code 85025 or 85027 and 85007 will be billed as needed.
Performed by
PCL Clinical Laboratory - Flow Cytometry
Ordering |
Performed
Daily
Methodology
Flow Cytometry
Reported
Same day
Synonyms
- LAB3053
- TBCP
Result Interpretation |
Reference Interval
Component | Sex | From Age | To Age | Normal Low | Normal High | Units |
B CELLS: %CD19+ | M/F | 3 | 21 | 11 | 45 | % |
M/F | 21 | 150 | 1 | 12 | % | |
B CELLS: ABS CD19+ | M/F | 3 | 21 | 432 | 3345 | /uL |
M/F | 21 | 150 | 42 | 400 | /uL | |
CD4:CD8 RATIO | M/F | 0 | 150 | 1.0 | 3.3 | % |
HELPER T CELLS: %CD3+CD4+ | M/F | 3 | 21 | 33 | 44 | % |
M/F | 21 | 150 | 32 | 66 | % | |
HELPER T CELLS: ABS CD3+CD4+ | M/F | 3 | 21 | 1216 | 2009 | /uL |
M/F | 21 | 150 | 389 | 1868 | /uL | |
SUPPRESSOR T CELL:ABS CD3+CD8+ | M/F | 3 | 21 | 351 | 2479 | /uL |
M/F | 21 | 150 | 185 | 1049 | /uL | |
SUPPRESSOR T CELLS: %CD3+CD8+ | M/F | 3 | 21 | 8 | 31 | % |
M/F | 21 | 150 | 14 | 42 | % | |
TOTAL T CELLS: %CD3+ | M/F | 3 | 21 | 55 | 82 | % |
M/F | 21 | 150 | 63 | 86 | % | |
TOTAL T CELLS: ABS CD3+ | M/F | 3 | 21 | 2649 | 3639 | /uL |
M/F | 21 | 150 | 760 | 2130 | /uL |
Performed by
PCL Clinical Laboratory - Flow Cytometry
Administrative |
CPT Codes
86355, 86359, 86360