QUANTIFERON - TB Gold

LAB3516

Collect

Special Set of Collection Tubes (obtained from PCL Clinical Lab)

Collect 1 mL into each of 3 Cellestis QuantiFERON Gold collection tubes for High Altitude (HA). Carefully follow the instructions below:

  1. Tubes must be drawn in the following order:  
    1. GRAY (Nil HA)
    2. RED (Antigen HA)
    3. PURPLE (Mitogen HA)
  2. If other blood work is requested, collect the QuantiFERON tubes LAST. If a butterfly is used, use a purge (throw away) tube so the tubing does not take up any of the required 1 mL volume in the first tube.
  3. Draw 1 ml into each tube and fill exactly up to the black line. **DO NOT OVERFILL OR UNDERFILL TUBES**.
    • Expect relatively slow blood draw time; keep tube and needle in place for 30 seconds after tube appears to have completed filling to ensure correct volume is collected.
  4. Shake tubes firmly 10 times just enough to ensure the entire inner surface of the tube is coated with blood and to dissolve the antigens on the tube walls.
    • Avoid overly vigorous shaking- it may cause gel disruption and aberrant results.
  5. Label each of the three tubes and include the time of collection.
  6. Maintain tubes at ambient temperature.

 

 

 

Unacceptable Conditions

  1. Samples received after 5:00pm Monday-Friday
  2. Samples collected or received on Saturday or Sunday
  3. Incorrect collection tubes
  4. Refrigerated or frozen samples.
  5. Tubes that are over- or underfilled
  6. Specimens not properly identified

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature before 5 PM Monday-Friday, same day of collection.

Offsite: Obtain collection containers from PCL Clinical lab, collect samples as indicated and return to PCL Clinical lab for processing the same day of collection before 5:00pm Monday-Friday.

Stability (from collection to initiation)

Ambient: 16 hours

Remarks

Samples may only be submitted to PCL Clinical Lab Mon-Fri BEFORE 5:00pm. Sample must be processed within 16 hours of collection.

Performed by

PCL Clinical Laboratory - Special Chemistry

Performed

Friday

Methodology

Lymphocytes Stimulation and ELISA

(Assay FDA approved for in vitro diagnostic use.)

Reported

Friday and Monday

Synonyms

  • Interferon Gamma Release Assay
  • LAB3516

Reference Interval

Negative

Performed by

PCL Clinical Laboratory - Special Chemistry

CPT Codes

86480

Collection

LAB3516

Collect

Special Set of Collection Tubes (obtained from PCL Clinical Lab)

Collect 1 mL into each of 3 Cellestis QuantiFERON Gold collection tubes for High Altitude (HA). Carefully follow the instructions below:

  1. Tubes must be drawn in the following order:  
    1. GRAY (Nil HA)
    2. RED (Antigen HA)
    3. PURPLE (Mitogen HA)
  2. If other blood work is requested, collect the QuantiFERON tubes LAST. If a butterfly is used, use a purge (throw away) tube so the tubing does not take up any of the required 1 mL volume in the first tube.
  3. Draw 1 ml into each tube and fill exactly up to the black line. **DO NOT OVERFILL OR UNDERFILL TUBES**.
    • Expect relatively slow blood draw time; keep tube and needle in place for 30 seconds after tube appears to have completed filling to ensure correct volume is collected.
  4. Shake tubes firmly 10 times just enough to ensure the entire inner surface of the tube is coated with blood and to dissolve the antigens on the tube walls.
    • Avoid overly vigorous shaking- it may cause gel disruption and aberrant results.
  5. Label each of the three tubes and include the time of collection.
  6. Maintain tubes at ambient temperature.

 

 

 

Unacceptable Conditions

  1. Samples received after 5:00pm Monday-Friday
  2. Samples collected or received on Saturday or Sunday
  3. Incorrect collection tubes
  4. Refrigerated or frozen samples.
  5. Tubes that are over- or underfilled
  6. Specimens not properly identified

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature before 5 PM Monday-Friday, same day of collection.

Offsite: Obtain collection containers from PCL Clinical lab, collect samples as indicated and return to PCL Clinical lab for processing the same day of collection before 5:00pm Monday-Friday.

Stability (from collection to initiation)

Ambient: 16 hours

Remarks

Samples may only be submitted to PCL Clinical Lab Mon-Fri BEFORE 5:00pm. Sample must be processed within 16 hours of collection.

Performed by

PCL Clinical Laboratory - Special Chemistry

Ordering

Performed

Friday

Methodology

Lymphocytes Stimulation and ELISA

(Assay FDA approved for in vitro diagnostic use.)

Reported

Friday and Monday

Synonyms

  • Interferon Gamma Release Assay
  • LAB3516
Result Interpretation

Reference Interval

Negative

Performed by

PCL Clinical Laboratory - Special Chemistry

Administrative

CPT Codes

86480

QUINIDINE

LAB192

 

Collect

Plain red. Also acceptable: Green (sodium heparin).

Specimen Preparation

Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL) For serum, ensure complete clot formation has taken place prior to centrifugation.

Unacceptable Conditions

Gray (sodium fluoride/potassium oxalate). Hemolyzed specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: 8 hours; Refrigerated: 7 days; Frozen: 14 days

Performed

Sun-Sat

Methodology

Immunoassay

Reported

Within 24 hours

Synonyms

  • Cardioquin
  • Duraquin
  • LAB192
  • QUINIDINE-AR
  • QUIN

Reference Interval

1.5-4.5 µg/mL
Toxic: 10.1 µg/mL or greater

CPT Codes

80194
Collection

LAB192

 

Collect

Plain red. Also acceptable: Green (sodium heparin).

Specimen Preparation

Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL) For serum, ensure complete clot formation has taken place prior to centrifugation.

Unacceptable Conditions

Gray (sodium fluoride/potassium oxalate). Hemolyzed specimens.

Storage/Transport Temperature

Frozen.

Stability (from collection to initiation)

After separation from cells: Ambient: 8 hours; Refrigerated: 7 days; Frozen: 14 days
Ordering

Performed

Sun-Sat

Methodology

Immunoassay

Reported

Within 24 hours

Synonyms

  • Cardioquin
  • Duraquin
  • LAB192
  • QUINIDINE-AR
  • QUIN
Result Interpretation

Reference Interval

1.5-4.5 µg/mL
Toxic: 10.1 µg/mL or greater
Administrative

CPT Codes

80194