JAK2 [V617F] MUTATION BY PCR

LAB3164

 

Collect

Lavender (EDTA) OR bone marrow (EDTA).

Specimen Preparation

Do not freeze. Transport 5 mL whole blood (Min: 1 mL) OR 3 mL bone marrow (Min: 1 mL).

Unacceptable Conditions

Serum. Specimens collected in anticoagulants other than EDTA. Frozen specimens. Clotted or grossly hemolyzed specimens.

Storage/Transport Temperature

Ambient

Stability (from collection to initiation)

Ambient: 72 hours; Refrigerated: 5 days; Frozen: Unacceptable

Performed by

Children's Hospital Colorado - Molecular Diagnostics Laboratory     

Performed

5 days a week

Methodology

Polymerase Chain Reaction

Reported

2-7 days

Synonyms

  • LAB3164
  • JAK2 Mutation V617F
  • JAK2 V617F
  • JAK2MUTPCR
  • JAK

Ordering Recommendations

Aids in the workup of suspected myeloproliferative neoplasms. Detects the JAK2 V617F mutation in peripheral blood or bone marrow.

Reference Interval

By report

Performed by

Children's Hospital Colorado - Molecular Diagnostics Laboratory     

CPT Codes

81270

Collection

LAB3164

 

Collect

Lavender (EDTA) OR bone marrow (EDTA).

Specimen Preparation

Do not freeze. Transport 5 mL whole blood (Min: 1 mL) OR 3 mL bone marrow (Min: 1 mL).

Unacceptable Conditions

Serum. Specimens collected in anticoagulants other than EDTA. Frozen specimens. Clotted or grossly hemolyzed specimens.

Storage/Transport Temperature

Ambient

Stability (from collection to initiation)

Ambient: 72 hours; Refrigerated: 5 days; Frozen: Unacceptable

Performed by

Children's Hospital Colorado - Molecular Diagnostics Laboratory     

Ordering

Performed

5 days a week

Methodology

Polymerase Chain Reaction

Reported

2-7 days

Synonyms

  • LAB3164
  • JAK2 Mutation V617F
  • JAK2 V617F
  • JAK2MUTPCR
  • JAK

Ordering Recommendations

Aids in the workup of suspected myeloproliferative neoplasms. Detects the JAK2 V617F mutation in peripheral blood or bone marrow.

Result Interpretation

Reference Interval

By report

Performed by

Children's Hospital Colorado - Molecular Diagnostics Laboratory     

Administrative

CPT Codes

81270

JC VIRUS BY PCR

LAB3255

 

Collect

All sample types, except blood and urine, in a sterile container.

Specimen source is required.

If submitting a Pink top tube (EDTA) refer to test code JCPCRPLS;  if submitting a urine refer to test code JCPCRUR.

Pediatric Collection

0.5 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; contaminated specimen; clotted sample; gross hemolysis; leaking containers; samples containing fixatives.

For plasma, refer to test code JCPCRPLS; for urine, refer to test code JCPCRUR.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if delivery is less than 72 hours. If delivery is not expected within 72 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours; Refrigerated: 72 hours; Frozen (-70 degrees C): 6 months.

Remarks

Qualitative results will be given for tissues, stools, swabs, or diluted samples. Please submit one requisition per sample.

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Performed

Tuesdays and Thursdays

Methodology

DNA amplification by polymerase chain reaction (PCR) using a JC-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Synonyms

  • LAB3255

Interpretive Data

Qualitative testing:

TND = Target Not Detected (negative)

Positive = Virus Detected

Quantitative testing:

TND = Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001 - 1,000,000 copies/mL = Quantitative range

>1,000,000 copies/mL = Greater than maximum quantitative range

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

CPT Codes

87799

Collection

LAB3255

 

Collect

All sample types, except blood and urine, in a sterile container.

Specimen source is required.

If submitting a Pink top tube (EDTA) refer to test code JCPCRPLS;  if submitting a urine refer to test code JCPCRUR.

Pediatric Collection

0.5 mL minimum volume.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; contaminated specimen; clotted sample; gross hemolysis; leaking containers; samples containing fixatives.

For plasma, refer to test code JCPCRPLS; for urine, refer to test code JCPCRUR.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if delivery is less than 72 hours. If delivery is not expected within 72 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours; Refrigerated: 72 hours; Frozen (-70 degrees C): 6 months.

Remarks

Qualitative results will be given for tissues, stools, swabs, or diluted samples. Please submit one requisition per sample.

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Ordering

Performed

Tuesdays and Thursdays

Methodology

DNA amplification by polymerase chain reaction (PCR) using a JC-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Synonyms

  • LAB3255
Result Interpretation

Interpretive Data

Qualitative testing:

TND = Target Not Detected (negative)

Positive = Virus Detected

Quantitative testing:

TND = Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001 - 1,000,000 copies/mL = Quantitative range

>1,000,000 copies/mL = Greater than maximum quantitative range

Performed by

PCL Clinical Laboratory - Molecular Diagnostics

Administrative

CPT Codes

87799

JC VIRUS BY PCR ON PLASMA

LAB3234

 

Collect

One 6.0 mL pink top, plasma (EDTA).  Tubes must be filled to at least 80% capacity.

Specimen source must be included on requisition.

Pediatric Collection

Transport at least 0.5 mL of whole blood collected in a 1.0 mL pink top microtainer (EDTA) or other blood tube with EDTA anticoagulant.

Unacceptable Conditions

Sample not properly identified; insufficient sample volume; contaminated specimen; clotted sample; tubes not filled to 80-100% capacity; improper storage conditions; gross hemolysis; samples stored in fixatives.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if tested within 72 hours. If delivery is not expected within 72 hours, centrifuge, transfer supernatant to an aliquot tube, and freeze at -70 degrees C. Ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours; Refrigerated: 72 hours; Frozen (-70 degrees C): 6 months.

Remarks

Quantitative results will be reported.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Performed

Mon - Fri

Methodology

DNA amplification by polymerase chain reaction (PCR) using a JC-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Synonyms

  • LAB3234

Interpretive Data

TND - Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001-1,000,000 copies/mL = Quantitative ranges

>1,000,000 copies/mL = Greater than maximum quantitative range.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

CPT Codes

87799

Collection

LAB3234

 

Collect

One 6.0 mL pink top, plasma (EDTA).  Tubes must be filled to at least 80% capacity.

Specimen source must be included on requisition.

Pediatric Collection

Transport at least 0.5 mL of whole blood collected in a 1.0 mL pink top microtainer (EDTA) or other blood tube with EDTA anticoagulant.

Unacceptable Conditions

Sample not properly identified; insufficient sample volume; contaminated specimen; clotted sample; tubes not filled to 80-100% capacity; improper storage conditions; gross hemolysis; samples stored in fixatives.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Send at 2-8 degrees C if tested within 72 hours. If delivery is not expected within 72 hours, centrifuge, transfer supernatant to an aliquot tube, and freeze at -70 degrees C. Ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours; Refrigerated: 72 hours; Frozen (-70 degrees C): 6 months.

Remarks

Quantitative results will be reported.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Ordering

Performed

Mon - Fri

Methodology

DNA amplification by polymerase chain reaction (PCR) using a JC-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Synonyms

  • LAB3234
Result Interpretation

Interpretive Data

TND - Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001-1,000,000 copies/mL = Quantitative ranges

>1,000,000 copies/mL = Greater than maximum quantitative range.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Administrative

CPT Codes

87799

JC VIRUS BY PCR ON URINE

LAB3312

 

Collect

Urine submitted in a sterile container.

Specimen source must be included on requisition.

Pediatric Collection

At least 0.5 mL of urine in a sterile container.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; contaminated specimen; improper storage conditions; no fixatives allowed.

Storage/Transport Temperature

Internal:  Deliver to lab immediately.
Offsite:  Send at 2-8 degrees C if tested within 72 hours. If delivery is not expected within 72 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours; Refrigerated: 72 hours; Frozen (-70 degrees C): 6 months.

Remarks

Quantitative results will be given.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Performed

Mon - Fri

Methodology

DNA amplification by polymerase chain reaction (PCR) using a JC-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Synonyms

  • LAB3312

Interpretive Data

TND = Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001 - 1,000,000 copies/mL = Quantitative ranges

>1,000,000 copies/mL = Greater than maximum quantitative range.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

CPT Codes

87799

Collection

LAB3312

 

Collect

Urine submitted in a sterile container.

Specimen source must be included on requisition.

Pediatric Collection

At least 0.5 mL of urine in a sterile container.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; contaminated specimen; improper storage conditions; no fixatives allowed.

Storage/Transport Temperature

Internal:  Deliver to lab immediately.
Offsite:  Send at 2-8 degrees C if tested within 72 hours. If delivery is not expected within 72 hours, freeze at -70 degrees C and ship on dry ice.

Stability (from collection to initiation)

Ambient: 12 hours; Refrigerated: 72 hours; Frozen (-70 degrees C): 6 months.

Remarks

Quantitative results will be given.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Ordering

Performed

Mon - Fri

Methodology

DNA amplification by polymerase chain reaction (PCR) using a JC-specific DNA probe detected by real-time PCR.

Reported

24-48 hours

Synonyms

  • LAB3312
Result Interpretation

Interpretive Data

TND = Target Not Detected (negative)

POS<1000 = Positive. Virus detected below 1000 copies/mL

1001 - 1,000,000 copies/mL = Quantitative ranges

>1,000,000 copies/mL = Greater than maximum quantitative range.

Performed by

PCL Clinical Laboratory - Molecular Diagnosis

Administrative

CPT Codes

87799