VON WILLEBRAND FACTOR ACTIVITY

LAB3539

Collect

One 2.7 mL Blue top (sodium citrate 3.2%). Fill tube to at least 90% capacity.

All blue tops for coagulation assays which do not have a blood culture collected first must have a discard tube collected prior to filling.  Discard tube should be another blue top in which greater than 1 mL of blood is collected.

Pediatric Collection

0.5 mL of platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample diluted with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma from cells. Place plasma in aliquot container and transport to lab frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 4 hours.

Plasma aliquot: Refrigerated, 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70C), 6 months.

Remarks

Reference range established using 3.2% sodium citrate. vWd Factor activity (Ristocetin Cofactor) refers to a specific site on the non-covalently bound complex of "Factor VIII molecules" circulating in plasma. This site must be present in order for platelets to agglutinate in the presence of ristocetin. vWd Factor Activity is frequently decreased in von Willebrand disease.

Performed by

PCL Clinical Laboratory - Coagulation

Performed

Daily, day shift

Methodology

Immunoturbidimetric assay

Reported

Same day

Synonyms

  • VWF Activity
  • LAB3539

Reference Interval

Effective 01/11/2016:

38 - 152%

Before 01/11/2016:

49.0-194.0 U/dL

Interpretive Data

Interpretation of results under 50.0 U/dL will be done in conjunction with patient's ABO blood typing.

Performed by

PCL Clinical Laboratory - Coagulation

CPT Codes

85245

Collection

LAB3539

Collect

One 2.7 mL Blue top (sodium citrate 3.2%). Fill tube to at least 90% capacity.

All blue tops for coagulation assays which do not have a blood culture collected first must have a discard tube collected prior to filling.  Discard tube should be another blue top in which greater than 1 mL of blood is collected.

Pediatric Collection

0.5 mL of platelet poor plasma.

Unacceptable Conditions

Sample not properly identified; incorrect container; insufficient sample volume; more than 4 hours since collection; clotted sample; hemolysed sample; tube fill volume less than 90% or greater than 110%; sample diluted with I.V. fluid.

Storage/Transport Temperature

Internal: Deliver to lab immediately.
Offsite: Centrifuge and separate platelet poor plasma from cells. Place plasma in aliquot container and transport to lab frozen.

Stability (from collection to initiation)

Whole blood: Ambient, 4 hours.

Plasma aliquot: Refrigerated, 4 hours; Frozen (-20 degrees C), 2 weeks; Frozen (-70C), 6 months.

Remarks

Reference range established using 3.2% sodium citrate. vWd Factor activity (Ristocetin Cofactor) refers to a specific site on the non-covalently bound complex of "Factor VIII molecules" circulating in plasma. This site must be present in order for platelets to agglutinate in the presence of ristocetin. vWd Factor Activity is frequently decreased in von Willebrand disease.

Performed by

PCL Clinical Laboratory - Coagulation

Ordering

Performed

Daily, day shift

Methodology

Immunoturbidimetric assay

Reported

Same day

Synonyms

  • VWF Activity
  • LAB3539
Result Interpretation

Reference Interval

Effective 01/11/2016:

38 - 152%

Before 01/11/2016:

49.0-194.0 U/dL

Interpretive Data

Interpretation of results under 50.0 U/dL will be done in conjunction with patient's ABO blood typing.

Performed by

PCL Clinical Laboratory - Coagulation

Administrative

CPT Codes

85245

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