VITAMIN B1, WHOLE BLOOD

LAB125

Collect

Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation

Protect from light within one hour of collection and during storage and shipment. Transfer 3 mL whole blood to an ARUP Amber Transport Tube. (Min: 0.6 mL)

Unacceptable Conditions

Any specimen other than whole blood. Glass tubes. Clotted or non-frozen specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 6 months

Notes

Whole blood is the preferred specimen for thiamine assessment.  Approximately 80 percent of thiamine present in whole blood is found in red blood cells. 

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

2-4 days

Synonyms

  • Thiamine
  • TPP
  • VITB1
  • Thiamin Pyrophosphate
  • B1 Vitamin
  • Thiamine Diphosphate
  • TDP
  • LAB125

Ordering Recommendations

Use for nutritional assessment of vitamin B1 (thiamine).

Reference Interval

70-180 nmol/L

Interpretive Data

This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.

CPT Codes

84425
Collection

LAB125

Collect

Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation

Protect from light within one hour of collection and during storage and shipment. Transfer 3 mL whole blood to an ARUP Amber Transport Tube. (Min: 0.6 mL)

Unacceptable Conditions

Any specimen other than whole blood. Glass tubes. Clotted or non-frozen specimens.

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Stability (from collection to initiation)

Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 6 months

Notes

Whole blood is the preferred specimen for thiamine assessment.  Approximately 80 percent of thiamine present in whole blood is found in red blood cells. 
Ordering

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

2-4 days

Synonyms

  • Thiamine
  • TPP
  • VITB1
  • Thiamin Pyrophosphate
  • B1 Vitamin
  • Thiamine Diphosphate
  • TDP
  • LAB125

Ordering Recommendations

Use for nutritional assessment of vitamin B1 (thiamine).
Result Interpretation

Reference Interval

70-180 nmol/L

Interpretive Data

This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.

Administrative

CPT Codes

84425

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