VITAMIN A [RETINOL A]

LAB580

Collect

Green (sodium or lithium heparin), plasma separator tube, or serum separator tube. Also acceptable: Lavender (EDTA) or pink (K2EDTA).

Patient Preparation

Patient should fast for 12 hours and abstain from alcohol for 24 hours prior to collection.

Specimen Preparation

Separate serum or plasma within 1 hour of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube immediately. (Min: 0.2 mL) Avoid hemolysis.

Unacceptable Conditions

Whole blood or body fluids other than serum or plasma.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 month; Frozen: 1 year

Notes

Serum retinol is typically maintained until hepatic stores are almost depleted. Values greater than 0.30 mg/L represent adequate liver stores, whereas values less than 0.10 mg/L indicate deficiency. Samples that come in contact with plastic tubing or have been exposed to excessive light may show low results.

Vitamin A toxicity occurs when retinol concentration exceeds the capacity of retinol binding protein (RBP). Individuals with compromised renal function can retain RBP and may, therefore, have moderate retinol elevations. Drugs which interfere with vitamin A analysis include probucol (Lorelco).

This assay does not measure other vitamin A metabolites such as retinaldehyde and retinoic acid.

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

1-4 days

Ordering Recommendations

Use for nutritional assessment of vitamin A (retinol and retinyl palmitate) in serum or plasma.

Reference Interval

Components
Reference Interval
Vitamin A (Retinol)
Age
Reference Interval
0-1 month
2 months-12 years
13-17 years
18 years and older
0.18-0.50 mg/L
0.20-0.50 mg/L
0.26-0.70 mg/L
0.30-1.20 mg/L

Vitamin A (Retinyl Palmitate)0-150 years: 0-0.10 mg/L

CPT Codes

84590
Collection

LAB580

Collect

Green (sodium or lithium heparin), plasma separator tube, or serum separator tube. Also acceptable: Lavender (EDTA) or pink (K2EDTA).

Patient Preparation

Patient should fast for 12 hours and abstain from alcohol for 24 hours prior to collection.

Specimen Preparation

Separate serum or plasma within 1 hour of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube immediately. (Min: 0.2 mL) Avoid hemolysis.

Unacceptable Conditions

Whole blood or body fluids other than serum or plasma.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 month; Frozen: 1 year

Notes

Serum retinol is typically maintained until hepatic stores are almost depleted. Values greater than 0.30 mg/L represent adequate liver stores, whereas values less than 0.10 mg/L indicate deficiency. Samples that come in contact with plastic tubing or have been exposed to excessive light may show low results.

Vitamin A toxicity occurs when retinol concentration exceeds the capacity of retinol binding protein (RBP). Individuals with compromised renal function can retain RBP and may, therefore, have moderate retinol elevations. Drugs which interfere with vitamin A analysis include probucol (Lorelco).

This assay does not measure other vitamin A metabolites such as retinaldehyde and retinoic acid.
Ordering

Performed

Sun-Sat

Methodology

Quantitative High Performance Liquid Chromatography

Reported

1-4 days

Ordering Recommendations

Use for nutritional assessment of vitamin A (retinol and retinyl palmitate) in serum or plasma.
Result Interpretation

Reference Interval

Components
Reference Interval
Vitamin A (Retinol)
Age
Reference Interval
0-1 month
2 months-12 years
13-17 years
18 years and older
0.18-0.50 mg/L
0.20-0.50 mg/L
0.26-0.70 mg/L
0.30-1.20 mg/L

Vitamin A (Retinyl Palmitate)0-150 years: 0-0.10 mg/L

Administrative

CPT Codes

84590

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