Respiratory Culture (includes Gram Stain)

3277

Collect

2-5 mL of expectorated sputum, tracheal aspirate, induced sputum, BAL, or bronch brush in saline, in a sterile container.

Source must be indicated. Indicate any unusual suspected organisms.

For cystic fibrosis or lung transplant patients, order C RESPCF.

Pediatric Collection

2 mL minimum volume.

Unacceptable Conditions

Poor quality sputum (presence of greater than 10 epithelial cells/lpf); subsequent samples during the same 24 hour period; sample not properly identified; non-sterile or leaking  container.

Storage/Transport Temperature

Internal: Remove suction tubing top from sterile container and replace with cap included in package. Place each specimen in a sealed individual bag. Deliver to lab immediately at ambient temperature.
Offsite: Package as above and send at 2-8 degrees C within 24 hours of collection. Do not freeze.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 24 hours; Frozen: Unacceptable.

Remarks

Care provider will be notified of unacceptable specimens.

Identification and susceptibility testing will be performed according to evidence-based practices.

Cultures will be held for 7 days.

Performed by

PCL Clinical Laboratory - Microbiology

Notes

Gram stain, identification, and susceptibility tests are billed separately from culture.

Routine bacterial culture only. For other requests, order specific culture (e.g. C AFB for Mycobacterial culture).

Performed

Daily

Methodology

Standard microbiology procedures for bacterial stain, aerobic culture and identification.

Reported

Preliminary: 1 day

 

Final: Negative at 2 days

Synonyms

  • LAB3277

Interpretive Data

Usual respiratory flora

Performed by

PCL Clinical Laboratory - Microbiology

CPT Codes

87070 - Routine Bacterial Culture

Collection

3277

Collect

2-5 mL of expectorated sputum, tracheal aspirate, induced sputum, BAL, or bronch brush in saline, in a sterile container.

Source must be indicated. Indicate any unusual suspected organisms.

For cystic fibrosis or lung transplant patients, order C RESPCF.

Pediatric Collection

2 mL minimum volume.

Unacceptable Conditions

Poor quality sputum (presence of greater than 10 epithelial cells/lpf); subsequent samples during the same 24 hour period; sample not properly identified; non-sterile or leaking  container.

Storage/Transport Temperature

Internal: Remove suction tubing top from sterile container and replace with cap included in package. Place each specimen in a sealed individual bag. Deliver to lab immediately at ambient temperature.
Offsite: Package as above and send at 2-8 degrees C within 24 hours of collection. Do not freeze.

Stability (from collection to initiation)

Ambient: 2 hours; Refrigerated: 24 hours; Frozen: Unacceptable.

Remarks

Care provider will be notified of unacceptable specimens.

Identification and susceptibility testing will be performed according to evidence-based practices.

Cultures will be held for 7 days.

Performed by

PCL Clinical Laboratory - Microbiology

Notes

Gram stain, identification, and susceptibility tests are billed separately from culture.

Routine bacterial culture only. For other requests, order specific culture (e.g. C AFB for Mycobacterial culture).

Ordering

Performed

Daily

Methodology

Standard microbiology procedures for bacterial stain, aerobic culture and identification.

Reported

Preliminary: 1 day

 

Final: Negative at 2 days

Synonyms

  • LAB3277
Result Interpretation

Interpretive Data

Usual respiratory flora

Performed by

PCL Clinical Laboratory - Microbiology

Administrative

CPT Codes

87070 - Routine Bacterial Culture

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