EHRLICHIA CHAFFEENSIS AB G/M

LAB794

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.05 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

Human ehrlichiosis is a tick-borne disease caused by rickettsial-like agents. Two forms, human monocytic ehrlichiosis (HME) and human granulocytic ehrlichiosis (HGE), have been described. HME is often referred to as "spotless" or rashless Rocky Mountain spotted fever, and has been reported in various regions of the United States. The causative agent of HME has been identified as Ehrlichia chaffeensis. Infected individuals produce specific antibodies to Ehrlichia chaffeensis which can be detected by an immunofluorescent antibody (IFA) test.

Performed

Tue, Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-5 days

Synonyms

  • Human Monocytic Ehrlichiosis (HME), HME Antibody IgG & IgM
  • LAB794

Ordering Recommendations

Diagnose infection from Ehrlichia chaffeensis.

Reference Interval

Components
Reference Interval
Ehrlichia chaffeensis Antibody, IgG by IFA< 1:64  Negative-No significant level of Ehrlichia chaffeensis IgG antibody detected.
1:64-1:128  Equivocal-Questionable presence of Ehrlichia chaffeensis IgG antibody detected.  Repeat testing in 10-14 days may be helpful.
≥1:256  Positive-Presence of IgG antibody to Ehrlichia chaffeensis detected, suggestive of current or past infection.
Ehrlichia chaffeensis Antibody, IgM by IFA< 1:16  Negative-No significant level of Ehrlichia chaffeensis IgM antibody detected.
≥ 1:16  Positive-Presence of IgM antibody to Ehrlichia chaffeensis detected, suggestive of current or recent infection.

Interpretive Data

Refer to individual components.

CPT Codes

86666 x2
Collection

LAB794

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.05 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.

Unacceptable Conditions

Contaminated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

Human ehrlichiosis is a tick-borne disease caused by rickettsial-like agents. Two forms, human monocytic ehrlichiosis (HME) and human granulocytic ehrlichiosis (HGE), have been described. HME is often referred to as "spotless" or rashless Rocky Mountain spotted fever, and has been reported in various regions of the United States. The causative agent of HME has been identified as Ehrlichia chaffeensis. Infected individuals produce specific antibodies to Ehrlichia chaffeensis which can be detected by an immunofluorescent antibody (IFA) test.
Ordering

Performed

Tue, Fri

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Reported

1-5 days

Synonyms

  • Human Monocytic Ehrlichiosis (HME), HME Antibody IgG & IgM
  • LAB794

Ordering Recommendations

Diagnose infection from Ehrlichia chaffeensis.
Result Interpretation

Reference Interval

Components
Reference Interval
Ehrlichia chaffeensis Antibody, IgG by IFA< 1:64  Negative-No significant level of Ehrlichia chaffeensis IgG antibody detected.
1:64-1:128  Equivocal-Questionable presence of Ehrlichia chaffeensis IgG antibody detected.  Repeat testing in 10-14 days may be helpful.
≥1:256  Positive-Presence of IgG antibody to Ehrlichia chaffeensis detected, suggestive of current or past infection.
Ehrlichia chaffeensis Antibody, IgM by IFA< 1:16  Negative-No significant level of Ehrlichia chaffeensis IgM antibody detected.
≥ 1:16  Positive-Presence of IgM antibody to Ehrlichia chaffeensis detected, suggestive of current or recent infection.

Interpretive Data

Refer to individual components.

Administrative

CPT Codes

86666 x2

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