E. COLI SHIGA-LIKE TOXIN [EIA]

LAB1318

Collect

Stool. OR actively growing isolate of E. coli, in pure culture.

Specimen Preparation

Stool: Place 5 mL stool in enteric transport media (Cary-Blair) (ARUP Supply #29799) immediately after collection. Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 1 mL)
E. coli Isolate:
Transport sealed container with pure culture on agar slant or on a swab in bacterial transport media. Place each specimen in an individually sealed bag.

Unacceptable Conditions

Specimens or isolates from sources other than stool.

Storage/Transport Temperature

Stool: Refrigerated.
E. coli
Isolate:
Room temperature.

Stability (from collection to initiation)

Stool in Transport Media: Ambient: 1 hour; Refrigerated: 72 hours; Frozen: 1 week
E. coli
Isolate:
Ambient: 1 week; Refrigerated: 1 week; Frozen: Unacceptable

Notes

This test will identify the presence of E. coli Shiga-like toxin, however it cannot determine specific of strains of E. coli. (e.g., E. coli O157:H7)

Performed

Sun-Sat

Methodology

Qualitative Enzyme Immunoassay

Reported

Within 2 days

Synonyms

  • LAB1318
  • ECSLT AG

Additional Technical Information

Ordering Recommendations

Test detects the Shiga toxins, sensitive markers of toxigenic Escherichia coli. CDC recommends stool specimens also be cultured for E. coli 0157:H7 (0157 STEC).

Reference Interval

Negative

Interpretive Data

By report.

CPT Codes

87427
Collection

LAB1318

Collect

Stool. OR actively growing isolate of E. coli, in pure culture.

Specimen Preparation

Stool: Place 5 mL stool in enteric transport media (Cary-Blair) (ARUP Supply #29799) immediately after collection. Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 1 mL)
E. coli Isolate:
Transport sealed container with pure culture on agar slant or on a swab in bacterial transport media. Place each specimen in an individually sealed bag.

Unacceptable Conditions

Specimens or isolates from sources other than stool.

Storage/Transport Temperature

Stool: Refrigerated.
E. coli
Isolate:
Room temperature.

Stability (from collection to initiation)

Stool in Transport Media: Ambient: 1 hour; Refrigerated: 72 hours; Frozen: 1 week
E. coli
Isolate:
Ambient: 1 week; Refrigerated: 1 week; Frozen: Unacceptable

Notes

This test will identify the presence of E. coli Shiga-like toxin, however it cannot determine specific of strains of E. coli. (e.g., E. coli O157:H7)
Ordering

Performed

Sun-Sat

Methodology

Qualitative Enzyme Immunoassay

Reported

Within 2 days

Synonyms

  • LAB1318
  • ECSLT AG

Additional Technical Information

Ordering Recommendations

Test detects the Shiga toxins, sensitive markers of toxigenic Escherichia coli. CDC recommends stool specimens also be cultured for E. coli 0157:H7 (0157 STEC).
Result Interpretation

Reference Interval

Negative

Interpretive Data

By report.

Administrative

CPT Codes

87427

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