Borrelia burgdoferi Antibodies, Total w/reflex to IgG and IgM by Western Blot

LAB3222

 

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Unacceptable Conditions

CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

This panel is appropriate for Lyme disease testing less than 4 weeks from erythema migrans or onset of disease symptoms.

A negative result indicates the immunoblot evaluation for B. burgdorferi antibody demonstrates no antibodies unique to B. burgdorferi and is, therefore, not supportive of Lyme disease.

A positive result indicates that the immunoblot evaluation for B. burgdorferi antibody is consistent with the presence of antibody produced by patients in response to infection by B. burgdorferi and suggests the presence of Lyme disease. Although the test has been shown to have a high degree of reliability for diagnostic purposes, laboratory data should always be correlated with clinical findings.

Current CDC recommendations for the serological diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocals and positives with immunoblot. Both IgM and IgG immunoblots should be performed on specimens obtained less than 4 weeks after appearance of erythema migrans. Only IgG immunoblot is to be performed on specimens greater than 4 weeks after disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease.

If ELISA result is 1.00 LIV or greater, then IgG and IgM immunoblot will be added. Additional charges apply.

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Reported

1-3 days

Synonyms

  • LAB3222
  • LYME ACUTE

Ordering Recommendations

Preferred reflex test to detect Lyme disease in individuals with ≤4 weeks of clinical symptoms or exposure to tick. Positive/equivocal screen confirmed by immunoblot.

Reference Interval

Components
Reference Interval
Borrelia burgdorferi Antibodies, Total by ELISA
0.99 LIV or lessNegative - Antibody to B. burgdorferi not detected.
1.00-1.20 LIVEquivocal - Repeat testing in 10-14 days may be helpful.
1.21 LIV or greaterPositive - Probable presence of antibody to B. burgdorferi detected.

Borrelia burgdorferi Antibody, IgG by ImmunoblotEffective August 15, 2011

Negative
Borrelia burgdorferi Antibody, IgM by ImmunoblotEffective August 15, 2011

Negative

Interpretive Data

Refer to report.

CPT Codes

86618; if reflexed, add 86617 x2
Collection

LAB3222

 

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Unacceptable Conditions

CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Notes

This panel is appropriate for Lyme disease testing less than 4 weeks from erythema migrans or onset of disease symptoms.

A negative result indicates the immunoblot evaluation for B. burgdorferi antibody demonstrates no antibodies unique to B. burgdorferi and is, therefore, not supportive of Lyme disease.

A positive result indicates that the immunoblot evaluation for B. burgdorferi antibody is consistent with the presence of antibody produced by patients in response to infection by B. burgdorferi and suggests the presence of Lyme disease. Although the test has been shown to have a high degree of reliability for diagnostic purposes, laboratory data should always be correlated with clinical findings.

Current CDC recommendations for the serological diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocals and positives with immunoblot. Both IgM and IgG immunoblots should be performed on specimens obtained less than 4 weeks after appearance of erythema migrans. Only IgG immunoblot is to be performed on specimens greater than 4 weeks after disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease.

If ELISA result is 1.00 LIV or greater, then IgG and IgM immunoblot will be added. Additional charges apply.
Ordering

Performed

Sun-Sat

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot

Reported

1-3 days

Synonyms

  • LAB3222
  • LYME ACUTE

Ordering Recommendations

Preferred reflex test to detect Lyme disease in individuals with ≤4 weeks of clinical symptoms or exposure to tick. Positive/equivocal screen confirmed by immunoblot.
Result Interpretation

Reference Interval

Components
Reference Interval
Borrelia burgdorferi Antibodies, Total by ELISA
0.99 LIV or lessNegative - Antibody to B. burgdorferi not detected.
1.00-1.20 LIVEquivocal - Repeat testing in 10-14 days may be helpful.
1.21 LIV or greaterPositive - Probable presence of antibody to B. burgdorferi detected.

Borrelia burgdorferi Antibody, IgG by ImmunoblotEffective August 15, 2011

Negative
Borrelia burgdorferi Antibody, IgM by ImmunoblotEffective August 15, 2011

Negative

Interpretive Data

Refer to report.

Administrative

CPT Codes

86618; if reflexed, add 86617 x2

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